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Start Over Author "Duenes, Judith A." Publication Type Academic Journals
153 results on '"Duenes, Judith A."'

33. Contractile activity of mouse small intestinal longitudinal smooth muscle1,2 <FN ID="FN1"><NO>1</NO>Part of this work was presented as a poster at Digestive Disease Week on May 19, 2003 in Orlando, FL, and published as an abstract in Gastroenterology 2003;124:M1343.</FN> <FN ID="FN2"><NO>2</NO>This study was supported by NIH R01 DK 39337 (MGS), Mayo Foundation, and Dept. of Surgery, Tohoku University, Sendai, Japan.</FN>

Author

Ueno, Tatsuya, Duenes, Judith A., Kost, Louis J., and Sarr, Michael G.

Subjects
*TRANSPLANTATION of organs, tissues, etc., *SMOOTH muscle, *PHYSICAL therapy, *GENETICS
Abstract

: BackgroundInterest in genomic modulation experimentally often necessitates use of mouse models.: Materials and methodsAIM: To characterize and quantitate smooth muscle contractile activity of the mouse small intestine using in vitro techniques. Full-thickness jejunal and ileal muscle strips from mice were cut in the direction of longitudinal muscle, suspended in tissue baths (37°C), and connected to force transducers. Spontaneous contractility and two dose-response curves to the cholinergic agonist bethanechol and adrenergic agonist norepinephrine were quantitated for 6 h.: ResultsTotal contractile activity increased over 4 to 5 h in jejunum (P < 0.01) but not in ileum. Frequency of contractions (counts/min) in jejunum increased from 16 to 33 (P < 0.01) in the first 4 h, then remained stable; ileal frequency did not change. One hour of cold preservation had no major effect on contractile activity and frequency. Bethanechol increased and norepinephrine decreased contractile activity in dose-dependent fashion. The dose of bethanechol producing 50% increase in maximal response did not differ between the first and second dose-response; in contrast, the concentration of norepinephrine producing 50% decrease in activity for the second dose-response in jejunum was decreased compared to the first dose-response (P < 0.01). Cold preservation had no substantive effect on agonist responses.: ConclusionExperiments in murine jejunal but not ileal longitudinal muscle in vitro must consider early changes in contractile activity after tissue harvest. These experiments serve as a baseline for comparison of stimuli or genetic modifications on murine contractile activity of longitudinal muscle in vivo. [Copyright &y& Elsevier]

Published
2004
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