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Start Over Author "Dittrich, K" Publication Type Academic Journals
318 results on '"Dittrich, K"'

17. Suppression of neuropeptide Y1 receptor function in SK-N-MC cells by nitric oxide

Author

Dotsch, J., Hanze, J., Beste, O., Behrendt, J., Weber, W.M., Dittrich, K., and Rascher, W.

Subjects
Neuropeptide Y -- Research, Cell receptors -- Research, Nitric oxide -- Research, Vasodilators -- Research, Vasoconstrictors -- Research, Biological sciences
Abstract

Experiments were conducted to examine the direct influence of the vasodilator nitric oxide on NPY1- receptor function. SK-N-MC and CHP-234 cells expressing Y1 an Y2 receptor, respectively, were incubated with the NO donors sodium nitroprusside, 3-morpholinosydnonimine and S-nitroso-N-acetyl-penicillamine. Receptor binding, Y1-receptor mRNA expression by Northern blot and adenosine 3'5'-cyclic monophosphate and intracellular Ca2+ concentration responses were analyzed.

Published
1997

46. Retinol binding protein 4 (RBP4) is primarily associated with adipose tissue mass in children.

Author

FRIEBE, D., NEEF, M., ERBS, S., DITTRICH, K., KRATZSCH, J., KOVACS, P., BLÜHER, M., KIESS, W., and KÖRNER, A.

Subjects
RETINOL-binding proteins, ADIPOSE tissues, CHILDHOOD obesity, ADIPOKINES, INSULIN resistance, CARDIOVASCULAR diseases risk factors, FAT cells
Abstract

Objective. Retinol binding protein 4 (RBP4) is a novel adipocytokine that may link obesity and insulin resistance. We aimed to discriminate between primary and secondary associations of RBP4 with obesity and related disease. Design. We applied clinical and experimental approaches to investigate the association of RBP4 levels with normal development, obesity, metabolic and cardiovascular parameters in 68 lean and 61 obese children. Results. RBP4 significantly increased with age and pubertal development in healthy lean children. Obese children had significantly higher RBP4 levels compared with lean controls (30.5±1.4 vs. 26.3±1.1 mg/L, P<0.05) and there was a clear association with BMI independent of age (r=0.33, P<0.0001). RBP4 levels correlated significantly with parameters of lipid and glucose metabolism, as well as cardiovascular parameters in univariate analyses. Multiple regression analyses confirmed the strong association of RBP4 with BMI z-score and age, while the association with most metabolic and cardiovascular parameters was abolished. To assess whether the association of RBP4 with obesity may be attributable to adipogenesis, we evaluated RBP4 expression and secretion during adipocyte differentiation using the human SGBS cell line. In preadipocytes, RBP4 mRNA expression was nearly undetectable but increased during differentiation up to approximately 1600-fold (P<0.05). Likewise, RBP4 secretion was restricted to mature adipocytes, further indicating that RBP4 is strongly related to differentiation of adipocytes. Conclusion. RBP4 is a marker of adipose tissue mass and obesity already evident in children. The association of RBP4 with metabolic and cardiovascular sequelae of obesity appears to be secondary to the underlying relationship wtih body fat. [ABSTRACT FROM AUTHOR]

Published
2011
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50. Nitric Oxide Stimulates Adrenomedullin Secretion and Gene Expression in Endothelial Cells.

Author

Schoof, E., Dittrich, K., Hänze, J., Opherk, P., Dumke, K., Rascher, W., and Dötsch, J.

Subjects
*ADRENOMEDULLIN, *NITRIC oxide, *GENE expression, *CELLS, *RADIOIMMUNOASSAY
Abstract

Adrenomedullin, a peptide with vasorelaxant activity, stimulates nitric oxide (NO) synthesis. We tested whether or not NO regulates the function of the adrenomedullin system. Human umbilical vein endothelial cells (HUVEC) were incubated with the NO donors sodium nitroprusside (SNP), morpholinosydnonimine (SIN-1) and the phospodiesterase V inhibitor zaprinast. In HUVEC, adrenomedullin concentration in the supernatant was measured by radioimmunoassay and mRNA expression was studied by Northern blot and competitive quantitative PCR. SNP, SIN-1, and zaprinast (100 μmol/l each) significantly increased adrenomedullin concentration in the supernatant of HUVEC twofold. The same concentrations increased adrenomedullin mRNA expression four- to tenfold. Similar results were obtained by both quantitative PCR and Northern blot. Thus, NO donor exposure in vitro increases both adrenomedullin secretion and mRNA expression in a dose-dependent manner.Copyright © 2002 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Published
2002
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