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Introducción: Cuidados adecuados de nutrición clínica constituyen parte integral del completo tratamiento de los pacientes hospitalizados. Para la práctica correcta de la terapia nutricional, los profesionales del equipo de salud necesitan conocimientos específicos. Es de interés conocer el nivel de estos conocimientos en el Paraguay. Materiales y métodos: A 174 personas de hospitales universitarios del Paraguay (29% médicos, 29% estudiantes de sexto año de Medicina de la Universidad Nacional de Asunción, 11% farmacéuticos, 24% licenciados en enfermería, 7% nutricionistas) se suministró un cuestionario de selección múltiple de 20 puntos para determinar el nivel de conocimiento en nutrición según la calificación obtenida. Resultados: La calificación mediana de un total de 20 puntos fue de 6 (0-15). Los médicos obtuvieron una mediana de 6 (2-15), los estudiantes 7 (2-14), los farmacéuticos 7 (0-15), los licenciados en enfermería 3 (0-11) y las nutricionistas 9 (4-13). El puntaje de las nutricionistas fue significativamente mayor que el de los otros grupos (p < 0,005). Conclusión: La formación en nutrición clínica en el personal de blanco de hospitales universitarios en Paraguay no es completa. El nivel de conocimiento es superior en las nutricionistas.Background: Adequate clinical nutrition care is an integral part of the complete treatment of hospitalised patients, requiring specific knowledge from the health care team. The aim of this study is to assess, in Paraguay, the health care team ability in clinical care nutrition. Materials and methods: A survey was made including 174 people of Paraguay university hospitals (29% physicians, 29% medicine graduating students, 11% pharmaceutics, 24% nurses, 7% dieticians), by answering voluntarilya multiple choice questionnaire of 20 items. Results: The median score of the 20 questions was 6 (0-15). Physicians obtained a median of 6 (2-15), graduating students 7 (2-14), pharmaceutics 7 (0-15), nurses 3 (0-11), and dieticians 9 (4-13). The dieticians obtained a significantly higher score than the other groups (p

We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope's existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. OPT acquisitions are performed on in vivo zebrafish embryos to demonstrate the implementations' viability.

Background: Adequate clinical nutrition care is an integral part of the complete treatment of hospitalised patients, requiring specific knowledge from the health care team. The aim of this study is to assess, in Paraguay, the health care team ability in clinical care nutrition., Materials and Methods: A survey was made including 174 people of Paraguay university hospitals (29% physicians, 29% medicine graduating students, 11% pharmaceutics, 24% nurses, 7% dieticians), by answering voluntarily a multiple choice questionnaire of 20 items., Results: The median score of the 20 questions was 6 (0-15). Physicians obtained a median of 6 (2-15), graduating students 7 (2-14), pharmaceutics 7 (0-15), nurses 3 (0-11), and dieticians 9 (4-13). The dieticians obtained a significantly higher score than the other groups (p < 0.005)., Conclusions: The knowledge about clinical nutrition in the health care members from university hospitals is not adequate. The level of education in clinical nutrition is better in the dietician.