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As the world becomes increasingly interconnected, digital technologies impact the way humans experience places and spaces in (smart) cities. To some extent, these smart technologies are intertwined with the urban living enabling the augmentation of the city's space and creating new opportunities for playful urban engagement. Moreover, touristic cities are becoming the arena of novel hospitality services, emerging from the widespread use of digital technologies and internet-based platforms. At the same time, visitors look for increasingly "authentic" travel experiences that provide meaningful interactions with locals. New business models, such as those emerging from the sharing economy, flourished in response to these fresh needs and opportunities. However, establishing points of dialogue that bring benefits to both residents and tourists is a complex task that still needs inquiry. Here we extend on previous efforts by proposing a playful immersive experience that focuses on creating an esthetically engaging environment for direct information exchange between locals and visitors. We investigate how technology can mediate and augment such information sharing, engaging tourists in a playful and immersive experience. Results of an experiment with 15 users are analyzed and presented to provide new insights to the HCI community. [ABSTRACT FROM AUTHOR]

The increase of smart buildings with Building Information Modeling (BIM) and Building Management Systems (BMS) has created a large amount of data, including those coming from sensors. These data are intended for monitoring the building conditions by authorized personnel, not being available to all building occupants. In this paper, we evaluate, from a qualitative point of view, if a user interface designed for a specific community can increase occupants' context-awareness about environmental issues within a building, supporting them to make more informed decisions that best suit their needs. We designed a user interface addressed to the student community of a smart campus, adopting an Iterative Design Cycle methodology, and engaged 48 students by means of structured interviews with the aim of collecting their feedback and conducting a qualitative analysis. The results obtained show the interest of this community in having access to information about the environmental data within smart campus buildings. For example, students were more interested in data about temperature and brightness, rather than humidity. As a further result of this study, we have extrapolated a series of design recommendations to support the creation of map-based user interfaces that we found to be effective in such contexts. [ABSTRACT FROM AUTHOR]

Internal sustainability efforts (ISE) refer to a wide range of internal corporate policies focused on employees. They promote, for example, work–life balance, gender equality, and a harassment-free working environment. At times, however, companies fail to keep their promises by not publicizing truthful reports on these practices, or by overlooking employees' voices on how these practices are implemented. To partly fix that, we developed a deep-learning framework that scored four fifths of the S&P 500 companies in terms of six ISEs, and a web-based system that engages users in a learning and reflection process about these ISEs. We evaluated the system in two crowdsourced studies with 421 participants, and compared our treemap visualization with a baseline textual representation. We found that our interactive treemap increased by up to 7% our participants' opinion change about ISEs, demonstrating its potential in machine-learning driven visualizations. [ABSTRACT FROM AUTHOR]

Biostimulants improve yield, quality, and stress acclimation in crops. In this work, we tested the possibility of using phenolics-rich extracts from spelt (Triticum dicoccum L.) husks to attenuate the effects of salt stress (100–200 mM NaCl) in maize. Two methanolic extracts were prepared from the soluble-conjugated (SC), and the insoluble-bound (IB) phenolic acid fractions of the spelt husk, and their effects were investigated on several stress-associated biochemical parameters, such as proline, lipid peroxidation, H 2 O 2 , GSH levels, and ion content. Results show that SC and IB fractions of husk extracts behaved very differently, no doubt due to their greatly divergent chemical composition, as revealed by both GC-MS and HPLC analyses. The efficacy of treatments in mitigating salt stress was also dose- and timing-dependent. IB, even at the lower concentration tested, was able to recover the performance of stressed plants in terms of growth, photosynthetic pigments content, and levels of salt stress markers. Recovery of shoot growth to control levels and reduction of stress-induced proline accumulation occurred regardless of whether plants were pre-treated or post-treated with IB, whereas only pre-treatment with the higher dose of IB was effective in mitigating oxidative stress. Although in some cases SC and even methanol alone exerted some positive effects, they could also be deleterious whereas IB never was. Overall, results indicate that a polyphenol-containing extract obtained from spelt by-products can behave as biostimulant in maize plants and can mitigate their response to salt stress, by acting on different biochemical targets. Image 1 • Two polyphenol-enriched extracts were prepared from spelt by-products. • Chemical composition of the two extracts was assessed by HPLC-DAD and GC-MS analysis. • Maize plants subjected to salt stress were treated with polyphenol-enriched extracts. • The efficacy of spelt husk extracts as biostimulant on maize plants was evaluated. [ABSTRACT FROM AUTHOR]

I-152 combines two pro-glutathione (GSH) molecules, namely N-acetyl-cysteine (NAC) and cysteamine (MEA), to improve their potency. The co-drug efficiently increases/replenishes GSH levels in vitro and in vivo; little is known about its mechanism of action. Here we demonstrate that I-152 not only supplies GSH precursors, but also activates the antioxidant kelch-like ECH-associated protein 1/nuclear factor E2-related factor 2 (KEAP1/NRF2) pathway. The mechanism involves disulfide bond formation between KEAP1 cysteine residues, NRF2 stabilization and enhanced expression of the γ-glutamil cysteine ligase regulatory subunit. Accordingly, a significant increase in GSH levels, not reproduced by treatment with NAC or MEA alone, was found. Compared to its parent compounds, I-152 delivered NAC more efficiently within cells and displayed increased reactivity to KEAP1 compared to MEA. While at all the concentrations tested, I-152 activated the NRF2 pathway; high doses caused co-activation of activating transcription factor 4 (ATF4) and ATF4-dependent gene expression through a mechanism involving Atf4 transcriptional activation rather than preferential mRNA translation. In this case, GSH levels tended to decrease over time, and a reduction in cell proliferation/survival was observed, highlighting that there is a concentration threshold which determines the transition from advantageous to adverse effects. This body of evidence provides a molecular framework for the pro-GSH activity and dose-dependent effects of I-152 and shows how synergism and cross reactivity between different thiol species could be exploited to develop more potent drugs. [ABSTRACT FROM AUTHOR]

Gastric cancer (GC) is one of the most common and lethal cancers. Alterations in the ubiquitin (Ub) system play key roles in the carcinogenetic process and in metastasis development. Overexpression of transcription factors YY1, HSF1 and SP1, known to regulate Ub gene expression, is a predictor of poor prognosis and shorter survival in several cancers. In this study, we compared a primary (23132/87) and a metastatic (MKN45) GC cell line. We found a statistically significant higher expression of three out of four Ub coding genes, UBC, UBB and RPS27A, in MKN45 compared to 23132/87. However, while the total Ub protein content and the distribution of Ub between the conjugated and free pools were similar in these two GC cell lines, the proteasome activity was higher in MKN45. Ub gene expression was not affected upon YY1, HSF1 or SP1 small interfering RNA (siRNA) transfection, in both 23132/87 and MKN45 cell lines. Interestingly, the simultaneous knockdown of UBB and UBC mRNAs reduced the Ub content in both cell lines, but was more critical in the primary GC cell line 23132/87, causing a reduction in cell viability due to apoptosis induction and a decrease in the oncoprotein and metastatization marker β-catenin levels. Our results identify UBB and UBC as pro-survival genes in primary gastric adenocarcinoma 23132/87 cells. [ABSTRACT FROM AUTHOR]