The present study aimed to validate the Leap Motion Controller (LMC)-based Fizyosoft® HandROM System developed by our team to evaluate range of motion (ROM) for fingers, wrist, and forearm in a new clinical setting. Thirty-five healthy individuals participated in the study (all right-handed, 20-30 years old). The LMC-based Fizyosoft® HandROM System is a licensed software ROM-measurement developed by our team. Pronation/supination, wrist flexion/extension, ulnar/radial deviation and metacarpophalangeal (MCP) flexion/extension of all fingers were measured with both the Fizyosoft® HandROM System and a universal goniometer. No significant differences were found between the two measurement methods for almost all mean ROMs except for ulnar and radial deviation (p > 0.05). Highly significant correlations were found between all ROMs of the forearm, wrist, and thumb (p < 0.01). MCP flexion showed significant correlation only in the index finger (r = 0.516, p = 0.003) and little finger (r = 0.517, p = 0.004). Besides, for both measures, the intraclass correlations were good to excellent on all ROMs of the forearm, wrist, and fingers except for MCP of the middle and ring fingers (0.68-0.88). The present study results indicated that the LMC-based Fizyosoft® HandROM System could sensitively track changes in the active motion of the thumb, wrist, and forearm. It is a viable alternative for assessing ROMs of the forearm, wrist, and thumb in patient follow-up., (Copyright © 2021 SFCM. Published by Elsevier Masson SAS. All rights reserved.)