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Copyright of MESO is the property of Zadruzna Stampa D.D. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract We have shown that the microtopography (mT) underlying colon cancer changes as a tumor de-differentiates. We distinguish the well-differentiated mT based on the increasing number of "pits" and poorly differentiated mT on the basis of increasing number of "posts." We investigated Rho A as a mechanosensing protein using mT features derived from those observed in the ECM of colon cancer. We evaluated Rho A activity in less-tumorogenic (Caco-2 E) and more tumorigenic (SW620) colon cancer cell-lines on microfabricated pits and posts at 2.5 μm diameter and 200 nm depth/height. In Caco-2 E cells, we observed a decrease in Rho A activity as well as in the ratio of G/F actin on surfaces with either pits or posts but despite this low activity, knockdown of Rho A led to a significant decrease in confined motility suggesting that while Rho A activity is reduced on these surfaces it still plays an important role in controlling cellular response to barriers. In SW620 cells, we observed that Rho A activity was greatest in cells plated on a post microtopography which led to increased cell motility, and an increase in actin cytoskeletal turnover.

Over the last few decades, we have gained insight into how researchers attempted to modify some natural molecules to be utilized as prospective agents for cancer treatment. Many scientists synthesized new natural compounds by incorporating specific functional groups and metals that improved their antitumor activity while reducing undesirable side effects. In this investigation, we synthesized four novel structurally modified enones that differ in the functional groups attached to the carbonyl group of the enone system (methyl - E1; isopropyl - E2; isobutyl - E3; and cyclopropyl - E4) and explored their anticancer potential against human carcinoma of the colon HCT-116, the cervical HeLa, and normal lung cells MRC-5. From the findings, all the newly synthesized enones exhibited potent cytotoxic activity against the cancer cells while normal cells remained unharmed, with varying potencies among the various enones. We employed the MTT assay to assess enones's (E1-E4) cytotoxic effects, IC50 values and selectivity index in tumor cells. Furthermore, the newly synthesized enones induced cell death in cancer cells through apoptosis by promoting changes in cellular morphology, activating apoptotic regulators Bax and caspase 3, and inhibiting Bcl-2. The enones induced changes in the mitochondrial membrane potential, a release of cytochrome c, and a cell cycle arrest at the G2/M phase, thus inhibiting the growth of cancer cells. In conclusion, we demonstrated the anticancer potential of newly synthesized enones as promising candidates for future cancer treatments, especially for colon cancer, due to their selective cytotoxicity against these cancer cells. Further, in vivo studies are warranted to explore their full therapeutic potential., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

In polarized cells, the precise regulation of protein transport to and from the plasma membrane is crucial to maintain cellular function. Dysregulation of intracellular protein transport in neurons can lead to neurodegenerative diseases such as Retinitis Pigmentosa, Alzheimer's and Parkinson's disease. Here we used the light-dependent transport of the TRPL (transient receptor potential-like) ion channel in Drosophila photoreceptor cells to study the role of Rab proteins in TRPL recycling. TRPL is located in the rhabdomeric membrane of dark-adapted flies, but it is transported out of the rhabdomere upon light exposure and localizes at the Endoplasmatic Reticulum within 12 h. Upon subsequent dark adaptation, TRPL is recycled back to the rhabdomeric membrane within 90 min. To screen for Rab proteins involved in TRPL recycling, we established a tissue specific (ts) CRISPR/Cas9-mediated knock-out of individual Rab genes in Drosophila photoreceptors and assessed TRPL localization using an eGFP tagged TRPL protein in the intact eyes of these mutants. We observed severe TRPL recycling defects in the knockouts of Rab3 , Rab4 , Rab7 , Rab32 , and RabX2 . Using immunohistochemistry, we further showed that Rab3 and RabX2 each play a significant role in TRPL recycling and also influence TRPL transport. We localized Rab3 to the late endosome in Drosophila photoreceptors and observed disruption of TRPL transport to the ER in Rab3 knock-out mutants. TRPL transport from the ER to the rhabdomere ensues from the trans-Golgi where RabX2 is located. We observed accumulated TRPL at the trans-Golgi in RabX2 knock-out mutants. In summary, our study reveals the requirement of specific Rab proteins for different steps of TRPL transport in photoreceptor cells and provides evidence for a unique retrograde recycling pathway of TRPL from the ER via the trans-Golgi., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Zeger, Stanisławczyk, Bulić, Binder and Huber.)

While basal cell carcinoma is the most common type of skin cancer in humans, its subepidermal presentation is extremely rare. The risk factors for basal cell carcinoma development are well-known, but it remains unclear in which setting the tumor restricts itself to the dermal compartment. We present the fifth known case of subepidermal basal cell carcinoma. However, this particular presentation is unique due to arising beneath a capillary malformation. The patient had previously undergone multiple laser treatments which yielded no success. Initially, the vascular malformation was removed and sent for histopathological diagnosis. After the discovery of basal cell carcinoma, wide surgical resection was performed. The patient had no recurrence up to the last follow-up at 18 months postoperatively. This case demonstrates a new presentation of a very rare condition, but also highlights the importance of histopathological examination and the need for future research on any possible association between laser therapy and carcinogenesis.

While fasciotomy is the only urgent treatment option for compartment syndrome, the resulting open wound leaves room for complications. Closure of the wound can be done by different techniques, including split-thickness skin grafts, negative pressure therapy, an absorbable barbed suture system and a cable ties system. The aim of this paper is to demonstrate how a combined application of these methods can reduce their respective individual disadvantages. Our combined method was tried in two patients, one with an open tibial fracture and the other who underwent ulnar nerve reparation. Both patients started exhibiting signs of compartment syndrome within 3 hrs after surgery. Firstly, absorbable barbed suture systems were positioned with the running intradermal technique. Following this the cable ties were inserted and the limb in question was placed in an elevated position. Complete closure of the patient's wounds was achieved within 2 weeks without complications. This result is a testament to the added benefit of a combination of these methods in comparison with the results they produce individually.

Bathing Water Directive (2006/7/EC) specifies two reference methods for Escherichia coli detection: ISO 9308-1 and 9308-3. The revised ISO 9308-1 is recommended only for waters with a low bacterial background flora. Considering the extended time needed for analysis and, generally, the lack of experience in using ISO 9308-3 in the Mediterranean, the suitability of ISO 9308-1 for the examination of E. coli in bathing water was evaluated. The present study was aimed at a comparison of data obtained by the reference method in seawater samples (110 beaches, N=477) with data received from six alternative methods. Results show that recently used TSA/TBA method may overestimate E. coli numbers in marine waters. The temperature modified ISO 9308-1 (44°C) did not significantly alter the results, but outperformed the antibiotic supplemented agar at reducing non-E. coli bacteria on the plates, allowing the use of the respective method for monitoring coastal water., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

We studied the outcome of fetuses in whom cystic hygroma was diagnosed in the first and early second-trimester of pregnancy using transvaginal ultrasonography. The purpose of this study was to evaluate the association between fetal cystic hygroma and fetal cytogenetic abnormalities, and the long-term prognosis. Thirty-five consecutive fetuses between 9.1 and 13.4 weeks of gestation diagnosed as having a nuchal hygroma were evaluated ultrasonographically and karyotyped. Those with a normal chromosome complement were ultrasonographically monitored throughout the remainder of the pregnancy to document the resolution of the hygroma. Eighteen of thirty-five fetuses were found to have a normal karyotype and five of these were aborted electively. The hygromas resolved in ten of these karyotypically normal fetuses within four weeks of initial diagnosis and they were phenotypically normal at birth. Seventeen fetuses were karyotypically abnormal with trisomy twenty-one being the most common abnormality. Prenatal cytogenetic analysis should be offered to women with fetal cystic hygroma diagnosed in the first trimester. A normal outcome is likely in those without chromosome abnormalities.

Transabdominal placental biopsy under ultrasound guidance was carried out in 260 cases in the second trimester and 50 cases in the third trimester of pregnancy. Placental tissue was aspirated using an 18 or 20 gauge needle. In a total of 310 placental biopsies in the second and third trimester, 100 were performed because of suspicious ultrasonographic findings. Placental biopsy is simple in the presence of severe oligohydramnios where fetal blood sampling is usually more difficult. Oligohydramnios and polyhydramnios were the ultrasonographic findings in 50% of cases and were found to be associated with 30% of abnormal chromosomal findings. There was one (0.3%) abortion within two weeks following placental biopsy. Placental biopsy did not affect the outcome of the pregnancy.

In 126 pregnant women of the gestational age from the 28th to the 44th gestational week, the biparietal diameter, the abdomen diameter, and the placental maturity after Granum et al. were determined. Amniocentesis was performed for obstetric indication. Also the L/S ratio and the cytological estimation of the gestational age were recorded. The first degree of the placental maturity was found in 37, the second degree in 3, and the third degree in 36 pregnant women. In the first group, BPP was 8.38 +/- 0.75, the abdomen diameter 8.30 +/- 0.63, and pulmonary maturity in 73% of women according to the L/S ratio and in 91.9% according to cytological estimation. In the second group, BPP was 9.15 +/- 0.65, the abdomen diameter 9.03 +/- 0.83, and in 77.9% of women the L/S ratio was higher than 2 but in 56.6% of women, according to cytological estimation, there was a good pulmonary maturity. At the third degree of placental maturity, BPP was 9.49 +/- 0.60, the abdomen diameter 9.63 +/- 0.92, and a good pulmonary maturity in 94.4% of women. The RDS was not observed in 5.6% of newborns with the L/S ratio below 2. According to cytological estimation, BPP below 9.15 was found in pre-term labour and higher than 9.15 in at-term births when in 94% of women the L/S ratio was higher than 2.

A comparison was made between ultrasound and clinical findings in 45 patients with patients with adenomyosis. In patients with ovarian endometriosis ultrasound showed a cystic ovarian endometriosis ultrasound showed a cystic ovarian tumour in 27 patients, a mixed ovarian tumour in 5 patients, and a solid ovarian tumour in 3 patients, then polycystic ovaries in 5 patients, while in 4 patients the ultrasound finding was negative. In patients with adenomyosis, ultrasound suggested an uterus metriticus 9 times, a myoma 5 times, while cystic islets in the myometrium were found in two cases. The findings described are not specific to endometriosis.

Authors present a case of congenital sarcoma discovered by ultrasound at 30 weeks of pregnancy. The tumour was shown projecting from the anterior neck, well limited and measured 12 X 12 X 8 cm associated with maternal hydramnios. Pregnancy was terminated. Patho-histological finding was a spindle cell sarcoma of the neck and mandibular region. Discussion is about clinical, ultrasound-diagnostic and morphological aspect of this rare entity.

In addition to being the best method for the diagnosis of the hydatidiform mole, ultrasound is also used in the surgical treatment of the mole. The procedure of the evacuation of the complete hydatidiform mole by vacuum suction and continuous ultrasonic monitoring is described. The method is illustrated by three cases.

In the past five years cerclage was performed at the Department in 265 pregnant women (1.87% of all births). The outcome of pregnancy is presented in fifteen pregnant women in whom cerclage was performed with the reposition of the prolapsed fetal membranes. In five patients pregnancy was terminated on time, while four of them gave birth to premature infants, and the infants are alive. The total survival rate is 60%. In three of the women pregnancy was effected between the 29th and the 31st weeks, and their newborns died, whereas the remaining three women aborted before the 28th gestation week. The results justify the operation even in such unfavorable cases, as an attempt to maintain pregnancy.

The advances of simultaneous ultrasonic and vaginal or rectal examinations are described with regard to a better evaluation of the size, localization, and consistency of the process in the pelvis.

The frequency of various presentations and positions of the fetus in individual gestational weeks in the third trimester was examined in 1137 pregnant women based on 1785 ultrasonic findings. Cephalic presentation at 28 weeks was observed in 62% cases and at the end of pregnancy in 95%. Breech presentation decreased from 29% to 4% before delivery, and transverse presentation was 10 times less frequent at delivery than at the beginning of the third trimester. The first and second fetal positions occurred almost equally in both of the main longitudinal presentations. Dynamics of changes regarding position and presentation was examined in 32 patients regularly each week. In 10 of them there was no presentation change and in 22, changes occurred once or several times, tending to attain a physiologic cephalic presentation before delivery. This presentation, once attained, did not change after the 33rd week. With regard to breech presentation, changes are possible even up to the 36th week. The optimal time for ultrasonic examination with the purpose of determining fetal presentation and position is 33-37 full gestational weeks.