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This study examines the impact of engaging valuation specialists on the value relevance of Level 3 fair value measurements (FVMs) within the auditing process. Through an analysis of a sample of international commercial banks over the period from 2016 to 2019, this study reveals that both Level 1 and Level 2 FVMs are generally value relevant, regardless of the involvement of valuation specialists in the auditing process. However, the value relevance of Level 3 FVMs appears to be contingent upon the auditor's utilization of valuation specialists during the audit of these measurements. The findings of this study indicate that, notwithstanding the concerns highlighted in prior research, investors tend to hold the conviction that valuation specialists can make a substantive and positive impact on the auditing process, thereby enhancing the value relevance of Level 3 FVMs. Data Availability: Data are available from the public sources cited in the text. JEL Classifications: G21; M41; M42. [ABSTRACT FROM AUTHOR]

Abstract Background Sepsis is a troublesome syndrome that can cause intestinal injury and even high mortality rates. Omega-3 fatty acids (FAs) are known to protect against intestinal damage. Accordingly, the current study set out to explore if omega-3 FAs could affect sepsis-induced intestinal injury with the involvement of the microRNA (miR)-1-3p/Notch3-Smad axis. Methods First, cecal ligation and perforation (CLP) was performed to establish septic mouse models in C57BL/6J mice, and mouse intestinal epithelial MODE-K cells were induced by lipopolysaccharide (LPS) to establish sepsis cell models. The CLP-induced septic mice or LPS-exposed cells were subjected to treatment with Omega-3 FAs and activin (Smad signaling activator), miR-1-3p inhibitor and over-expressed/short hairpin RNA (oe-/sh)-Notch3 to explore their roles in inflammation, intestinal oxidative stress and cell apoptosis. A dual-luciferase reporter gene assay was further performed to verify the regulatory relationship between miR-1-3p and Notch3. Results Omega-3 FAs inhibited CLP-induced intestinal injury and ameliorated LPS-induced intestinal epithelial cell injury by down-regulating miR-1-3p, as evidenced by decreased levels of tumor necrosis factor-α, interleukin-1β (IL-1β) and IL-6, in addition to diminished levels of reactive oxygen species, malondialdehyde levels and superoxide dismutase activity. Furthermore, miR-1-3p could down-regulate Notch3, which inactivated the Smad pathway. Conclusion Collectively, our findings indicated that omega-3 FAs elevate the expression of Notch3 by down-regulating miR-1-3p, and then blocking the Smad pathway to alleviate intestinal epithelial inflammation and oxidative stress injury caused by sepsis.

In the three-degree-of-freedom virtual flight test, the scaled model has no translational motion, which will result in the model's dynamics being different from that of free flight, and this difference will directly affect its effectiveness in simulating free flight and the design of the flight control law. In this paper, the longitudinal and lateral-directional dynamic characteristics of virtual flight are studied from the three aspects of modal characteristics, disturbance response characteristics, and control response characteristics. The dynamic characteristic difference between the virtual flight test and free flight is analyzed. In addition, the scaling ratio of the model will also lead to differences in the flight control law design methods of virtual flight and full-size aircraft. The control law design method of the virtual flight test is established by studying the similarity relation of control law parameters between full-size aircraft and the scaled model, and the correctness of similar design of closed-loop control law parameters of the scaled model is verified by a virtual flight test. Finally, the attitude control law parameters obtained from the virtual flight test are applied to the six-degree-of-freedom flight simulation of a full-size aircraft, and the simulation results show that the three-axis attitude control law designed based on the virtual flight test also has good performance in the six-degree-of-freedom flight of the full-size aircraft, and the response of attitude angle and angular rate are approximately the same for both. Generally, the steady aerodynamic data of the aircraft are obtained through the traditional static wind tunnel test to construct the aerodynamic model of the aircraft. The aerodynamic model established by this method may not be able to accurately simulate the actual dynamic flight. However, the actual test flight of full-size aircraft has many disadvantages, such as high cost and high risk. To simulate real flight on the ground, the method of placing the scaled model in a wind tunnel for dynamic flight is proposed, which is called wind tunnel–based virtual flight testing (WTBVFT). The scaled model is connected with the strut through a three-degree-of-freedom rotation mechanism and installed in the wind tunnel test section, so that the model has three degrees of freedom of angular motion and no translational motion. This paper analyzes the differences in dynamics between virtual flight and free flight under open-loop and closed-loop control. The results show that the virtual flight test can simulate the angular motion of free flight well and can be used to design, verify, and simulate the attitude control law of full-size aircraft well. [ABSTRACT FROM AUTHOR]

In the early stages of aircraft design, a scaled model of the aircraft is installed in a wind tunnel for dynamic semi-free flight to approximate real flight, and the test data are then used to identify the aerodynamic parameters. However, the absence of the translational motion of the test model makes its flight dynamics different from those in free flight, and the effect of this difference on parameter identification needs to be investigated. To solve this problem, a 3-DOF wind tunnel virtual flight test device is built to fix the test model on a rotating mechanism, and the model is free to rotate in three axes through the deflection of the control surfaces. The flight dynamics equations for the wind tunnel virtual flight test are established and expressed as a decoupled form of the free flight force and the influence of the test support frame force on the model's motions through linearization. The differences between wind tunnel virtual flight and free flight are analysed to develop a model for the identification of aerodynamic parameters. The selection of the lateral-directional excitation signal and the design method of its parameters are established based on the requirements for the identifiability of the aerodynamic derivatives, and a step-by-step method for the identification of aerodynamic force and moment derivatives is established. The aerodynamic parameter identification results of a blended wing body aircraft show that the identification method proposed in this paper can obtain results with high accuracy, and the response of the modified motion model is consistent with that of the free flight motion model. [ABSTRACT FROM AUTHOR]

Slow cycling is a common feature shared among several stem cells (SCs) identified in adult tissues including hair follicle and cornea. Recently, existence of unipotent SCs in basal and lumenal layers of sweat gland (SG) has been described and label retaining cells (LRCs) have also been localized in SGs; however, whether these LRCs possess SCs characteristic has not been investigated further. Here, we used a H2BGFP LRCs system for in vivo detection of infrequently dividing cells. This system allowed us to specifically localize and isolate SCs with label-retention and myoepithelial characteristics restricted to the SG proximal acinar region. Using an alternative genetic approach, we demonstrated that SG LRCs expressed keratin 15 (K15) in the acinar region and lineage tracing determined that K15 labeled cells contributed long term to the SG structure but not to epidermal homeostasis. Surprisingly, wound healing experiments did not activate proximal acinar SG cells to participate in epidermal healing. Instead, predominantly non-LRCs in the SG duct actively divided, whereas the majority of SG LRCs remained quiescent. However, when we further challenged the system under more favorable isolated wound healing conditions, we were able to trigger normally quiescent acinar LRCs to trans-differentiate into the epidermis and adopt its long term fate. In addition, dissociated SG cells were able to regenerate SGs and, surprisingly, hair follicles demonstrating their in vivo plasticity. By determining the gene expression profile of isolated SG LRCs and non-LRCs in vivo, we identified several Bone Morphogenetic Protein (BMP) pathway genes to be up-regulated and confirmed a functional requirement for BMP receptor 1A (BMPR1A)-mediated signaling in SG formation. Our data highlight the existence of SG stem cells (SGSCs) and their primary importance in SG homeostasis. It also emphasizes SGSCs as an alternative source of cells in wound healing and their plasticity for regenerating different skin appendages.

Summary: During seed germination, cells embark on extensive post‐transcriptional and post‐translational modifications (PTM), providing a perfect platform to study these events in embryo rebooting from relative quiescenct to highly active state. PR‐619, a deubiquitylase inhibitor, delayed the rice seed germination and resulted in the accumulation of ubiquitylated proteins, which indicated the protein ubiquitylation is involved in this process. Using the K‐Ɛ‐GG antibody enrichment method integrated with high‐resolution mass spectrometry, a list of 2576 lysine ubiquitylated (Kub) sites in 1171 proteins was compiled for rice embryos at 0, 12 and 24 h after imbibition (HAI). Of these, the abundance of 1419 Kub sites in 777 proteins changed significantly. Most of them substantially increased within the first 12 HAI, which is similar to the dynamic state previously observed for protein phosphorylation, implying that the first 12 HAI are essential for subsequent switch during rice seed germination. We also quantitatively analyzed the embryo proteome in these samples. Generally, a specific protein's abundance in the ubiquitylome was uncorrelated to that in the proteome. The differentially ubiquitinated proteins were greatly enriched in the categories of protein processing, DNA and RNA processing/regulation related, signaling, and transport. The DiGly footprint of the Kub sites was significantly reduced on K48, a linkage typically associated with proteasome‐mediated degradation. These observations suggest ubiquitylation may modulate the protein function more than providing 26S degradation signals in the early stage of rice seed germination. Revealing this comprehensive ubiquitylome greatly increases our understanding of this critical PTM during seed germination. Significance Statement: During germination, the matured seeds experience a complex transition from relative static to active status. Protein ubiquitylation is involved in various cellular signaling processes. However, no systemic studies of ubiquitylation on seed germination have been conducted until now. This study comprehensively quantified the ubiquitylome and characterizes extensive modulation of ubiquitylation in the embryo during rice seed germination, which will facilitate the in‐depth functional exploration of ubiquitylation in plants. [ABSTRACT FROM AUTHOR]

In this paper, the bio-inspired blade motion is introduced to improve the propulsive performance of nano rotor at an ultra-low Reynolds number. However, the complex flow interacts with the flexible composite blade structure resulting in the change of nano rotor propulsion performance and the vibration of blade structure. A composite nano rotor with blade-pitch motion is investigated computationally with a computational solvers based on fluid–structure interaction. The finite element model for the composite rotor is created and verified with a non-contact modal test. It is found that the simulation results matched well with the experimental results. Successively, the propulsive performance of a rigid nano rotor is studied. The propulsive performance of the nano rotor is analysed at different bio-inspired pitch frequencies. The results show that the figure of merit of the bio-inspired pitch rotor increases because of the bio-inspired blade pitch motion. And it is also found that the improvement of the propulsive performance of the nano rotor varies with the pitch frequency. The propulsive performance of the flexible bio-inspired nano rotor is also studied with by using fluid–structure interaction method. It is found that the computational results for flexible nano rotor are lower than that for rigid nano rotor. It is evident that it is necessary to consider the flexibility of the composite nano rotor when investigating the propulsion performance of bio-inspired nano rotor. And the response of blade structure is also studied. Structural dynamic analysis shows that the blade structure vibrates with small amplitude. And two peak values are found at the rotation frequency and the fundamental frequency of the nano rotor structure. [ABSTRACT FROM AUTHOR]

The interaction between the upper and lower rotors greatly influences the aeroacoustic characteristics of a counter-rotating nano-coaxial rotor. To study this influence, a numerical investigation was carried out. The unsteady flow field of a single upper rotor was first studied with a large-eddy simulation computational fluid dynamics method coupled with a sliding-mesh technique. The Ffowcs Williams–Hawking equation method was used to investigate the aeroacoustic characteristics of the upper rotor based on the flow field. An experimental setup was established to validate the computational approach. The experimental results matched well with the computational results. Additionally, results show that the peak value of the total sound pressure level appeared near the blade tip, which verified that the tip vortex was one of the most important sources of rotor noise. Then the aeroacoustic noise of the nano-coaxial rotor was studied numerically. It was found that the total sound pressure level of the nano-coaxial rotor was greater than that of the upper rotor. Flow field analysis showed that the shedding vortices of the upper rotor interacted with the lower rotor, resulting in a blade–vortex interaction. It was evident that the aeroacoustic noise was enhanced by the interference between the upper and lower rotors. [ABSTRACT FROM AUTHOR]

Annona squamosa L. fruit played great anti-neoplastic activities. Its pericarps were discarded as waste. In this study, essential oil extracted from A. squamosa L. pericarps (APEO) was obtained by hydrodistillation and analysed by GC–MS. Furthermore, the anti-hepatoma activities and the underlying mechanism of the oil were firstly described. A total of 59 compounds were identified by Gas chromatography–mass spectrometry (GC–MS). The major compound in the oil was (−)-spathulenol (32.51%). The APEO demonstrated anti-hepatoma activity against SMMC-7721 hepatoma cell line with IC50 lower than 55 μg/mL. At the same time, nucleus shrinkage or broken were found in cells incubated with APEO through fluorescent microscope. In addition, pro-apoptosis and cell cycle arrest effects were confirmed by flow cytometry analysis. [ABSTRACT FROM AUTHOR]

Regulation of adult stem cells (SCs) is fundamental for organ maintenance and tissue regeneration. On the body surface, different ectodermal organs exhibit distinctive modes of regeneration and the dynamics of their SC homeostasis remain to be unraveled. A slow cycling characteristic has been used to identify SCs in hair follicles and sweat glands; however, whether a quiescent population exists in continuously growing nails remains unknown. Using an in vivo label retaining cells (LRCs) system, we detected an unreported population of quiescent cells within the basal layer of the nail proximal fold, organized in a ring-like configuration around the nail root. These nail LRCs express the hair stem cell marker, keratin 15 (K15), and lineage tracing show that these K15-derived cells can contribute to both the nail structure and peri-nail epidermis, and more toward the latter. Thus, this stem cell population is bifunctional. Upon nail plucking injury, the homeostasis is tilted with these SCs dominantly delivering progeny to the nail matrix and differentiated nail plate, demonstrating their plasticity to adapt to wounding stimuli. Moreover, in vivo engraftment experiments established that transplanted nail LRCs can actively participate in functional nail regeneration. Transcriptional profiling of isolated nail LRCs revealed bone morphogenetic protein signaling favors nail differentiation over epidermal fate. Taken together, we have found a previously unidentified ring-configured population of bifunctional SCs, located at the interface between the nail appendage organ and adjacent epidermis, which physiologically display coordinated homeostatic dynamics but are capable of rediverting stem cell flow in response to injury. [ABSTRACT FROM AUTHOR]

Question: How can a library-based bioinformatics support program be implemented and expanded to continuously support the growing and changing needs of the research community? Setting: A program at a health sciences library serving a large academic medical center with a strong research focus is described. Methods: The bioinformatics service program was established at the Norris Medical Library in 2005. As part of program development, the library assessed users' bioinformatics needs, acquired additional funds, established and expanded service offerings, and explored additional roles in promoting on- campus collaboration. Results: Personnel and software have increased along with the number of registered software users and use of the provided services. Conclusion: With strategic efforts and persistent advocacy within the broader university environment, library-based bioinformatics service programs can become a key part of an institution's comprehensive solution to researchers' ever-increasing bioinformatics needs. [ABSTRACT FROM AUTHOR]

Soil washing using an acid solution is a common practice for removing heavy metals from contaminated soil in Taiwan. However, serious loss of nutrients from soil is a major drawback of the washing. Distillery sludge can be used to prepare a dissolved organic matter (DOM) solution by extracting its organic constituents with alkaline solutions. This study employed DOM solutions to remediate Cd-contaminated soil (with concentrations up to 21.5 mg kg−1) and determine the factors affecting removal of Cd, such as pH, initial concentration of DOM solution, temperature, and washing frequency. When washing with pH 3.0 and 1250 mg L−1 DOM solution, about 80% and 81% of Cd were removed from the topsoil at 27 °C and subsoil at 40 °C, respectively. To summarize the changes in fertility during DOM washing with various pH solutions: the increase in organic matter content ranged from 7.7% to 23.7%; cation exchange capacity (CEC) ranged from 4.6% to 13.9%; available ammonium (N—NH4) content ranged from 39.4% to 2175%; and available phosphorus content ranged from 34.5% to 182%. Exchangeable K, Ca, and Mg remained in the topsoil after DOM washing, with concentrations of 1.1, 2.4, and 1.5 times higher than those treated with HCl solution at the same pH, respectively. [ABSTRACT FROM AUTHOR]

Background: Microarray data analysis presents a significant challenge to researchers who are unable to use the powerful Bioconductor and its numerous tools due to their lack of knowledge of R language. Among the few existing software programs that offer a graphic user interface to Bioconductor packages, none have implemented a comprehensive strategy to address the accuracy and reliability issue of microarray data analysis due to the well known probe design problems associated with many widely used microarray chips. There is also a lack of tools that would expedite the functional analysis of microarray results. Findings: We present Microarray ? US, an R-based graphical user interface that implements over a dozen popular Bioconductor packages to offer researchers a streamlined workflow for routine differential microarray expression data analysis without the need to learn R language. In order to enable a more accurate analysis and interpretation of microarray data, we incorporated the latest custom probe re-definition and re-annotation for Affymetrix and Illumina chips. A versatile microarray results output utility tool was also implemented for easy and fast generation of input files for over 20 of the most widely used functional analysis software programs. Conclusion: Coupled with a well-designed user interface, Microarray ? US leverages cutting edge Bioconductor packages for researchers with no knowledge in R language. It also enables a more reliable and accurate microarray data analysis and expedites downstream functional analysis of microarray results [ABSTRACT FROM AUTHOR]

We identify four novel DNA-binding complexes in the nuclear-encoded Lhcb1 promoter of the chlorophyte alga Dunaliella tertiolecta that are regulated by photosynthetic pathways in the plastid. The binding activities of three of the complexes were positively correlated with time-dependent changes in Lhcb1 transcript abundance, implicating their roles as transcriptional enhancers in a retrograde signal transduction pathway. Using a combination of inhibitors, uncouplers, and antimycin A, and by following the kinetic pattern of gene regulation, we infer two different sensors in the signal transduction pathway. On short time scales of 0.5 to about 4 h, the transthylakoid membrane potential appears to be a critical determinant of gene expression, whereas on time scales of 8 h or longer, the red ox state of the plastoquinone pool becomes increasingly more important. The differentiation of these two types of signals was observed in parallel effects on gene transcription and on the patterns of DNA-binding activities in the Lhcb1 promoter. These signals appear to be transduced at the nuclear level via a coordinated ensemble of DNA-binding complexes located between -367 and -188 bp from the start codon of the gene. The regulation of these elements allows the cell to up- or down-regulate the expression on Lhcb1 in response to changes in irradiance. [ABSTRACT FROM AUTHOR]

Tricodesmium sp. IMS 101, originally isolated from coastal western Atlantic waters by Prufert-Bebout and colleagues and maintained in sea-water based media, was successfully cultivated in two artificial media. Its characteristics of growth, nitrogen fixation, and regulation of nitrogen fixation were compared to those of natural populations and Trichodesmium sp. NIBB 1067. Results indicate that the culture grown in artificial media had nitrogen fixation characteristics similar to those when the culture is grown in sea-water based medium and to those of Trichodesmium sp. in the natural habitat. The study provides practical artificial media to facilitate the physiological studies of these important diazotropic cyanobacteria, as well as the cultivation of other Trichodesmium species in future studies. Manipulations of the light/dark cycle were performed to determine whether or not the daily cycle of nitrogen fixation is a circadian rhythm. Cultures grown under continuous light maintained the cycle for up to 6 days. We demonstrated that the daily cycle of nitrogen fixation in Trichodesmium sp. LMS 101 was at least partially under the control of circadian rhythm. [ABSTRACT FROM AUTHOR]

Investigates the cloning and transcription of the nitrogenase structural genes (nifUHDK) of the cyanobacteria Tricodesmium. Gene sequences; Transcription start site; Structures in the intercistronic regions of nifH and nifD; Results of hybridization.

Propofol and dexmedetomidine are both commonly used anaesthetics. Although they employ two different mechanisms to induce anaesthesia, both compounds influence the hippocampus and the HT22 cell line. HT22 cells are broadly used in neurobiological research. In this study, we assessed the effects of propofol and dexmedetomidine on signalling in HT22 cells. Using the SILAC (stable isotope labelling with amino acids in cell culture) labelling technique, IMAC (immobilized metal affinity chromatography) enrichment and high-resolution LC-MS/MS (liquid chromatography tandem mass spectrometry) analysis, we investigated the quantitative proteome and phosphoproteome in HT22 cells treated with propofol or dexmedetomidine. In total, 4,527 proteins and 6,824 phosphosites were quantified in cells treated with these two anaesthetics. With the assistance of intensive bioinformatics, the propofol and dexmedetomidine treatments were shown to induce distinct proteome and phosphoproteome profiles in HT22 cells. Consistent with our bioinformatics analysis, dexmedetomidine had a smaller effect than propofol on cell survival. These findings deepen our understanding of drug-induced anaesthesia. [ABSTRACT FROM AUTHOR]

In the modern ocean, a significant amount of nitrogen fixation is attributed to filamentous, nonheterocystous cyanobacteria of the genus Trichodesmium. In these organisms, nitrogen fixation is confined to the photoperiod and occurs simultaneously with oxygenic photosynthesis. Nitrogenase, the enzyme responsible for biological N[sub 2] fixation, is irreversibly inhibited by oxygen in vitro. How nitrogenaseis protected form damage by photosynthetically produces O[sub 2] was once an enigma. Using fast repetition rate fluorometry and fluorescence kinetic microscopy, we show that there is both temporal and spatial segregation of N[sub 2] fixation and photosynthesis within the photoperiod. Linear photosynthetic electron transport protects nitrogenase by reducing photosynthetically evolved O[sub 2] in photosystem I (PSI). We postulate that int he early evolutionary phase of oxygenic photosynthesis, nitrogenase served as an electron acceptor for anaerobic heterotophic metabolism and that PSI was favored by selection because it provided a micro-anaerobic environment for N[sub 2] fixation in cyanobacteria. [ABSTRACT FROM AUTHOR]

The mechanism of alleviating bleomycin-induced pulmonary fibrosis in mice was compared between Qingqiao(Forsythiae Fructus produced with immature fruits) and Laoqiao(Forsythiae Fructus produced with mature fruits) from the pharmacodynamic correlation and composition differences. Mice were randomized into normal, model, pirfenidone(50 mg·kg~(-1)), low-and high-dose(1.3, 2.6 g·kg~(-1), respectively) Qingqiao, and low-and high-dose(1.3, 2.6 g·kg~(-1), respectively) Laoqiao groups. The mouse model of pulmonary fibrosis was established by intratracheal instillation of bleomycin, during which the survival rate and body weight changes of the mice were measured. After modeling, the lung index was calculated, and the pathological changes in the lung tissue were evaluated by hematoxylin-eosin(HE), Masson, and Sirius red staining. Transmission electron microscopy was employed to observe the ultrastructure of the lung tissue. The biochemical assay was employed to measure the levels of transforming growth factor-β1(TGF-β1), α-smooth muscle actin(α-SMA), E-cadherin, and hydroxyproline(HYP) in the lung tissue and interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in the bronchoalveolar lavage fluid(BALF). The mRNA and protein levels of matrix metalloproteinase 7(MMP7), collagen Ⅰ, E-cadherin, TNF-α, vimentin, TGF-β1, and α-SMA in the lung tissue were determined by RT-qPCR and Western blot, respectively. The expression of α-SMA in the lung tissue was detected by the immunofluorescence assay. Principal component analysis was performed to compare the effects of Qingqiao and Laoqiao in ameliorating pulmonary fibrosis. Molecular docking was employed to analyze the binding between the compounds with high content in Laoqiao and TGF-β1. The cell-counting kit(CCK-8) assay was used to examine the effects of the active compounds on TGF-β1-induced BEAS-2B and HFL1 cell models. The results showed that Qingqiao and Laoqiao increased the survival rate, reduced the lung index, alleviated the pathological damage and collagen deposition in the lung tissue, ameliorated the damage of lamellar bodies in alveolar epithelial type Ⅱ cells, lowered the level of IL-6 and TNF-α in the BALF, down-regulated the expression of HYP, MMP7, vimentin, collagen Ⅰ, TGF-β1, and α-SMA, and up-regulated the expression of E-cadherin in the lung tissue of the mouse model of pulmonary fibrosis. The collagen deposition in the mouse model of pulmonary fibrosis was comprehensively evaluated by principal component analysis, and the effects of different treatments followed the trend of high-dose Laoqiao>low-dose Laoqiao>high-dose Qingqiao>low-dose Qingqiao. Molecular docking showed that hydroxytyrosol, caffeic acid, phillygenin, and(-)-lariciresinol had strong binding affinity with TGF-β1 receptor. The results of cell experiments showed that these compounds significantly attenuated the TGF-β1-induced damage in BEAS-2B cells and inhibited the TGF-β1-induced proliferation of HFL1 cells. In conclusion, both Qingqiao and Laoqiao were effective in ameliorating bleomycin-induced pulmonary fibrosis in mice. Laoqiao was superior to Qingqiao in reducing collagen deposition, which might be attributed to the higher content of hydroxytyrosol, caffeic acid, phillygenin, and(-)-lariciresinol in Laoqiao than in Qingqiao.

Previously, we reported that chromodomain Y-like (CDYL) acts as a crotonyl-coenzyme A hydratase and negatively regulates histone crotonylation (Kcr). However, the global CDYL-regulated crotonylome remains unclear. Here, we report a large-scale proteomics analysis for protein Kcr. We identify 14,311 Kcr sites across 3734 proteins in HeLa cells, providing by far the largest crotonylome dataset. We show that depletion of CDYL alters crotonylome landscape affecting diverse cellular pathways. Specifically, CDYL negatively regulated Kcr of RPA1, and mutation of the Kcr sites of RPA1 impaired its interaction with single-stranded DNA and/or with components of resection machinery, supporting a key role of RPA1 Kcr in homologous recombination DNA repair. Together, our study indicates that protein crotonylation has important implication in various pathophysiological processes., (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)

Dynamically tunable band stop filter based on metal-graphene metamaterials is proposed and numerically investigated at mid-infrared frequencies. The proposed filter is constructed by unit cells with simple gold strips on the stack of monolayer graphene and the substrate of BaF 2 . A stable modulation depth up to -23.26 dB can be achieved. Due to the cooperative effect of the "bright-bright" elements, the amount of the gold strips in each unit cell determines the number of the stop-bands, providing a simple and flexible approach to develop multispectral devices. Further investigations illustrate that the location of the stop bands not only can be adjusted by varying the length of gold strips, but also can be dynamically controlled by tuning the Fermi energy level of graphene, and deep modulation is acquired through designing the carrier mobility. With the sensitivity as high as 2393 nm/RIU of the resonances to the varieties of surrounding medium, the structure is also enabled to be an index based sensor. The results will benefit the on plane or integrated micro-structure research with simple structure and flexible tunability, and can be applied in multi-band stop filters, sensors and other graphene-based multispectral devices.

Grass carp (Ctenopharyngodon idellus) is an important worldwide commercial freshwater culture species. However, grass carp reovirus (GCRV) causes serious hemorrhagic disease in fingerlings and yearlings of fishes. To understand the molecular pathogenesis of host cells during GCRV infection, intensive proteomic quantification analysis of lysine acetylation in Ctenopharyngodon idella kidney (CIK) cells was performed. Using dimethylation labeling-based quantitative proteomics, 832 acetylated proteins with 1391 lysine acetylation sites were identified in response to GCRV infection, among which 792 proteins with 1323 sites were quantifiable. Bioinformatics analysis showed that differentially expressed lysine acetylated proteins are involved in diverse cellular processes and associated with multifarious functions, suggesting that extensive intracellular activities were changed upon viral infection. In addition, extensive alterations on host-protein interactions at the lysine acetylation level were also detected. Further biological experiments showed that the histone deacetylases (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) could significantly suppress the GCRV replication. To our knowledge, this is the first to reveal the proteome-wide changes in host cell acetylome with aquatic virus infection. The results provided in this study laid a basis for further understanding the host response to aquareovirus infection in the post-translational modification aspect by regulating cell lysine acetylation conducive to viral replication., Competing Interests: The authors declare no conflict of interest.

The data presented in this article are related to the publication entitled "Malonylome analysis of rhizobacterium Bacillus amyloliquefaciens FZB42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions"(doi:10.1016/j.jprot.2016.11.022) (B. Fan, Y. Li, L. Li et al.) [1]. This article presented the raw information of all malonyllysine sites identified by LC-MS/MS in the Bacillus amyloliquefaciens FZB42. Further, the functional features and conservation of the malonylated peptide/proteins were analyzed and made publicly available to enable critical or extended analyses.

The aim of this study was to assess the diagnostic value of RASSF1A methylation in renal cell carcinoma. Systematically search were performed using the Pubmed, ProQest and Web of Science for all articles on the association between RASSF1A methylation and renal cell carcinoma before 15 April 2015. After the filtration, 13 studies involving 677 cases and 497 controls met our criteria. Our meta-analysis suggested that hypermethylation of RASSF1A gene was associated with the increased risk of RCC(OR:4.14, 95%CI:1.06-16.1). Stratified analyses showed a similar risk in qualitative detection method(OR:28.4, 95%CI:10.2-79.6), body fluid sample(OR:12.8, 95%CI:5.35-30.8), and American(OR:10.5, 95%CI:1.97-55.9). Our result identified that RASSF1A methylation had a strong potential in prediction the risk of Renal cell carcinoma.

Objective: To explore relevant between human cytomegalovirus (HCMV) infection and college students' neurobehaviors., Methods: 87 college students were enlisted. They were tested with Bole. Neurobehavioral evaluation system (B. NES), and HCMV IgG antibody was detected after separation of serum. We analyzed the test results of B. NES by SPSS software., Results: 76 college students were infected by HCMV in the past and 11 college students were not infected. The infected group scored 8.89 +/- 6.60 in depression aspect of emotion state test, while control group got 15.73 +/- 9.00. There was Significant difference between infection group and control (P < 0.05). There were no significant differences in other aspects of emotion states, study and memory, perception and mental movement (P > 0.05)., Conclusion: HCMV infection is associated with depression status.