50 results on '"Benes Z"'
Search Results
2. THE CHAIR'S CORNER
- Author
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Farber, Laura V. and Aldana, Benes Z.
- Published
- 2012
3. When It Comes to Civility in Court, It's Do or Die.
- Author
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Aldana (Ret.), Judge Benes Z.
- Subjects
- *
CIVICS education , *COURTESY , *COURTS , *JUSTICE administration , *PROFESSIONALISM - Abstract
The article discusses the growing division and lack of civility in modern society, highlighting the need for respectful disagreement and the importance of civility in various settings, including politics, the courtroom, and the judicial system. It emphasizes the value of maintaining an environment of respect and professionalism, both for judges and all individuals involved in legal proceedings.
- Published
- 2023
4. THE CHAIR'S CORNER: HELPING RECENT GRADUATES
- Author
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Aldana, Benes Z.
- Published
- 2013
5. THE CHAIR'S CORNER: MOVING SOLOS FORWARD
- Author
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Aldana, Benes Z.
- Published
- 2012
6. Probiotic treatment with Probioflora in patients with predicted severe acute pancreatitis without organ failure
- Author
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van Baal, M.C., Kohout, P., Besselink, M.G., van Santvoort, H.C., Benes, Z., Zazula, R., Rijkers, G.T., and Gooszen, H.G.
- Published
- 2012
- Full Text
- View/download PDF
7. THE CHAIR'S CORNER: Think Forward As We Turn 50!
- Author
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Aldana, Benes Z.
- Published
- 2012
8. Get on the Board Lawyers and Nonprofits
- Author
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Aldana, Benes Z.
- Published
- 2004
9. THE CHAIRS' CORNER: THINK FORWARD, LOOK BACK
- Author
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Aldana, Benes Z.
- Published
- 2013
10. Thermal properties of carbon nanotubes and nanotube-based materials
- Author
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Hone, J., Llaguno, M.C., Biercuk, M.J., Johnson, A.T., Batlogg, B., Benes, Z., and Fischer, J.E.
- Published
- 2002
- Full Text
- View/download PDF
11. The Vibrational Density-of-States of Nanobundles of Single Wall Carbon Nanotubes: An Inelastic Neutron Scattering Study
- Author
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Rols, S., Benes, Z., Sauvajol, J. L., Papanek, P., Anglaret, E., Coddens, G., Dianoux, A. J., and Fischer, J. E.
- Published
- 1999
- Full Text
- View/download PDF
12. The Threats to Judicial Independence Are Myriad and Multiplying.
- Author
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Aldana (Ret.), Judge Benes Z.
- Subjects
- *
JUDICIAL selection & appointment , *JUDICIAL elections , *CONSERVATIVES , *JUDGES , *SUBPOENA - Abstract
The article reports that Montana's legislature passed a law that abolished the state's Judicial Nomination Commission which gave the new governor, and all future governors, unfettered power to fill judicial vacancies between elections. It mentions law abolishing the Judicial Nominating Commission was viewed as an effort by conservatives to remake what they saw as an activist judiciary and appoint or elect more conservative judges. It also mentions lawmakers issued an investigative subpoena.
- Published
- 2021
13. Low-temperature specific heat of single-wall carbon nanotubes
- Author
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Lasjaunias, J.C., Biljakovic, K., Benes, Z., and Fischer, J.E.
- Published
- 2002
- Full Text
- View/download PDF
14. Thermal Properties of Single-Walled Carbon Nanotubes
- Author
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Hone, J., Batlogg, B., Benes, Z., Llaguno, M. C., Nemes, N. M., Johnson, A. T., and Fischer, J. E.
- Published
- 2000
- Full Text
- View/download PDF
15. Mutational analysis of driver genes defines the colorectal adenoma: in situ carcinoma transition
- Author
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Jungwirth Jiri, Urbanova Marketa, Boot Arnoud, Hosek Petr, Bendova Petra, Siskova Anna, Svec Jiri, Kment Milan, Tumova Daniela, Summerova Sandra, Benes Zdenek, Buchler Tomas, Kohout Pavel, Hucl Tomas, Matej Radoslav, Vodickova Ludmila, van Wezel Tom, Vodicka Pavel, and Vymetalkova Veronika
- Subjects
Medicine ,Science - Abstract
Abstract A large proportion of colorectal carcinomas (CRC) evolve from colorectal adenomas. However, not all individuals with colonic adenomas have a risk of CRC substantially higher than those of the general population. The aim of the study was to determine the differences or similarities of mutation profile among low- and high-grade adenomas and in situ carcinoma with detailed follow up. We have investigated the mutation spectrum of well-known genes involved in CRC (such as APC, BRAF, EGFR, NRAS, KRAS, PIK3CA, POLE, POLD1, SMAD4, PTEN, and TP53) in a large, well-defined series of 96 adenomas and in situ carcinomas using a high-throughput genotyping technique. Besides, the microsatellite instability and APC and MLH1 promoter methylation were studied as well. We observed a high frequency of pathogenic variants in the studied genes. The APC, KRAS and TP53 mutation frequencies were slightly lower in adenoma samples than in in situ carcinoma samples. Further, when we stratified mutation frequency based on the grade, the frequency distribution was as follows: low-grade adenoma—high-grade adenomas—in situ carcinoma: APC gene 42.9–56.0–54.5%; KRAS gene 32.7–32.0–45.5%; TP53 gene 8.2–20.0–18.2%. The occurrence of KRAS mutation was associated with the presence of villous histology and methylation of the APC promoter was significantly associated with the presence of POLE genetic variations. However, no association was noticed with the presence of any singular mutation and occurrence of subsequent adenoma or CRC. Our data supports the multistep model of gradual accumulation of mutations, especially in the driver genes, such as APC, TP53 and KRAS.
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- 2022
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16. Quantized Phonon Spectrum of Single-Wall Carbon Nanotubes
- Author
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Benes, Z., Johnson, A. T., and Fischer, J. E.
- Subjects
Nanotechnology -- Research ,Carbon compounds -- Research ,Science and technology ,Research - Abstract
The electronic spectra of carbon nanotubes and other nanoscale systems are quantized because of their small radii. Similar quantization in the phonon spectra has been difficult to observe because of [...]
- Published
- 2000
17. MON-PP255: Percutaneous Endoscopic Gastrostomy in Children (One Center Experience)
- Author
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Kohout, K., Antos, Z., Puskarova, G., Rozmahel, M., Cermakova, D., and Benes, Z.
- Published
- 2015
- Full Text
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18. The alternatives to percutaneous endoscopic gastrostomy using pull method
- Author
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Kohout, P., Antoš, Z., Puškárová, G., Rozmahel, M., Černík, M., and Beneš, Z.
- Published
- 2013
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19. PP059-MON PROBIOTICS IN ACUTE PANCREATITIS
- Author
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Kohout, P., Cermakova, D., Benes, Z., and van Baal, M.
- Published
- 2013
- Full Text
- View/download PDF
20. Probiotic prophylaxis in patients with predicted severe acute pancreatitis without organ failure
- Author
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van Baal, M., Kohout, P., Besselink, M., van Santvoort, H., Benes, Z., Zazula, R., Rijkers, G., and Gooszen, H.
- Published
- 2012
- Full Text
- View/download PDF
21. P245 PERCUTANEOUS ENDOSCOPIC GASTROSTOMY IN NEUROLOGICAL DISEASES
- Author
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Kohout, P., Antos, Z., Puskarova, G., Rozmahel, M., Cernik, M., Ridzon, P., and Benes, Z.
- Published
- 2009
- Full Text
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22. PANCREAS ALERTS.
- Author
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He C., Zhang L., Shi W., Liang X., Ye Z., Zhang B., Liu S., van Baal, M. C., Kohout, P., Besselink, M. G., van Santvoort, H. C., Benes, Z., Zazula, R., Huai J. P., Sun X. C., Chen M. J., Jin Y., Ye X. H., Wu J. S., and Huang Z. M.
- Published
- 2012
23. Structural anisotropy of magnetically aligned single wall carbon nanotube films.
- Author
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Smith, B. W., Smith, B.W., Benes, Z., Luzzi, D. E., Luzzi, D.E., Fischer, J. E., Fischer, J.E., Walters, D. A., Walters, D.A., Casavant, M. J., Casavant, M.J., Schmidt, J., Smalley, R. E., and Smalley, R.E.
- Subjects
ANISOTROPY ,THICK films ,NANOSTRUCTURES - Abstract
Thick films of aligned single wall carbon nanotubes and ropes have been produced by filtration/deposition from suspension in strong magnetic fields. We measured mosaic distributions of rope orientations in the film plane, for samples of different thicknesses. For an ∼1 μm film the full width at half maximum (FWHM) derived from electron diffraction is 25°-28°. The FWHM of a thicker film (∼7 μm) measured by x-ray diffraction is slightly broader, 35±3°. Aligned films are denser than ordinary filter-deposited ones, and much denser than as-grown material. Optimization of the process is expected to yield smaller FWHMs and higher densities. © 2000 American Institute of Physics. [ABSTRACT FROM AUTHOR]
- Published
- 2000
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24. ChemInform Abstract: Super Dense LiC2 as a High Capacity Li Intercalation Anode.
- Author
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BINDRA, C., NALIMOVA, V. A., SKLOVSKY, D. E., BENES, Z., and FISCHER, J. E.
- Published
- 1998
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25. Author Correction: Mutational analysis of driver genes defines the colorectal adenoma: in situ carcinoma transition.
- Author
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Jungwirth J, Urbanova M, Boot A, Hosek P, Bendova P, Siskova A, Svec J, Kment M, Tumova D, Summerova S, Benes Z, Buchler T, Kohout P, Hucl T, Matej R, Vodickova L, van Wezel T, Vodicka P, and Vymetalkova V
- Published
- 2022
- Full Text
- View/download PDF
26. Improvements in colorectal cancer screening programmes - quantitative immunochemical faecal occult blood testing - how to set the cut-off for a particular population.
- Author
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Kovarova JT, Zavoral M, Zima T, Zak A, Kocna P, Kohout P, Granatova J, Vanickova Z, Vranova J, Suchanek S, Benes Z, Celko MA, and Povysil C
- Subjects
- Female, Humans, Immunologic Tests, Male, Middle Aged, ROC Curve, Sensitivity and Specificity, Colorectal Neoplasms diagnosis, Early Detection of Cancer, Occult Blood
- Abstract
Objective: The aim of the study was to determine the optimum cut-off value of the quantitative immunochemical test (q-FIT) OC-Sensor for colorectal cancer and advanced adenomatous polyps in a particular population., Methods: 815 patients were referred for colonoscopy and were offered two q-FIT examinations at two different colonoscopy centers. The patients were classified according to the colonoscopic findings. Test sensitivity, specificity, and accuracy were statistically evaluated using one test and two tests at the levels of 50, 75, 100, 125, and 150 ng/mL of faecal hemoglobin in those patients with advanced polyps and colorectal cancer. The optimum cut-off test level for clinically significant neoplasia was determined using one test., Results: The optimum cut-off value of q-FIT OC-Sensor for the detection of clinically significant neoplasia in our particular population was determined as 75 ng/mL using one test. This value provides an optimum proportion of 73% sensitivity (±95% CI 60.3% - 83.4%) and 90% specificity (±95% CI 86.8% - 92.8%), PPV and NPV were determined as 54.76% and 95.43% respectively., Conclusions: The first step in the implementation of q-FIT test in the screening program in our country is to determine the optimum cut-off level for a population, and to estimate the number of tests performed with respect to the optimum cost effectiveness and economical climate. Using one test, the optimum level of q-FIT OC-Sensor® in the Czech Republic was determined as 75 ng/mL. This study could serve as a model for further studies in other countries, where screening does not yet exist.
- Published
- 2012
- Full Text
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27. Calreticulin is a B cell molecular target in some gastrointestinal malignancies.
- Author
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Pekáriková A, Sánchez D, Palová-Jelínková L, Simsová M, Benes Z, Hoffmanová I, Drastich P, Janatková I, Mothes T, Tlaskalová-Hogenová H, and Tucková L
- Subjects
- Adenocarcinoma blood, Adult, Aged, Aged, 80 and over, Antibodies, Neoplasm blood, Antibody Specificity, Autoantibodies blood, Carcinoma, Hepatocellular blood, Colorectal Neoplasms blood, Enzyme-Linked Immunosorbent Assay, Epitopes immunology, Female, Gallbladder Neoplasms blood, Gallbladder Neoplasms immunology, Hepatitis C, Chronic blood, Hepatitis C, Chronic immunology, Humans, Immunoglobulin A blood, Immunoglobulin A immunology, Immunoglobulin G blood, Immunoglobulin G immunology, Liver Neoplasms blood, Male, Middle Aged, Pancreatic Neoplasms blood, Pancreatitis blood, Pancreatitis immunology, Young Adult, Adenocarcinoma immunology, Antibodies, Neoplasm immunology, Autoantibodies immunology, Autoantigens immunology, B-Lymphocytes immunology, Calreticulin immunology, Carcinoma, Hepatocellular immunology, Colorectal Neoplasms immunology, Liver Neoplasms immunology, Neoplasm Proteins immunology, Pancreatic Neoplasms immunology
- Abstract
Calreticulin, upon translocation to the cell surface, plays a critical role in the recognition of tumour cells and in experimentally induced cellular anti-tumour immunity. However, less is known about anti-calreticulin antibodies and their role in malignancies. Using enzyme-linked immunosorbent assay (ELISA), we found immunoglobulin (Ig)A and/or IgG anti-calreticulin antibodies in sera of approximately 63% of patients with hepatocellular carcinoma (HCC), 57% of patients with colorectal adenocarcinoma (CRA) and 47% of patients with pancreatic adenocarcinoma (PACA), while healthy controls, patients with viral hepatitis C and with chronic pancreatitis reached only 2%, 20% and 31% seropositivity, respectively. We found significantly elevated mean levels of IgA anti-calreticulin antibodies (P < 0.001) in patients with HCC (78.7 +/- 52.3 AU, mean +/- standard deviation), PACA (66.5 +/- 30.9 AU) and CRA (61.8 +/- 25.8 AU) when compared to healthy controls (41.4 +/- 19.2 AU). Significantly elevated mean levels of IgG anti-calreticulin antibodies (P < 0.001) were detected in patients with HCC (121.9 +/- 94.2 AU), gall bladder adenocarcinoma (118.4 +/- 80.0 AU) and PACA (88.7 +/- 55.6 AU) when compared to healthy controls (56.7 +/- 22.9 AU). Pepscan analysis revealed a large number of antigenic epitopes of calreticulin recognized by both IgA and IgG antibodies of patients with HCC and PACA, indicating robust systemic immune response. Moreover, significantly elevated levels of antibodies against peptide KGEWKPRQIDNP (P < 0.001) in these patients, tested by ELISA, confirmed the distinct character of antibody reactivity against calreticulin. The high occurrence and specificity of serum anti-calreticulin autoantibodies in the majority of patients with some gastrointestinal malignancies provide the evidence for their possible clinical relevance.
- Published
- 2010
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28. Comparison of morphological, immunohistochemical, and molecular genetic features of inflammatory fibroid polyps (Vanek's tumors).
- Author
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Daum O, Hatlova J, Mandys V, Grossmann P, Mukensnabl P, Benes Z, and Michal M
- Subjects
- Adult, Aged, Aged, 80 and over, Exons, Female, Gastrointestinal Tract pathology, Humans, Intestinal Diseases genetics, Male, Middle Aged, Mutation, Proto-Oncogene Proteins B-raf genetics, Stomach Diseases genetics, Intestinal Diseases pathology, Leiomyoma genetics, Leiomyoma pathology, Polyps genetics, Polyps pathology, Receptor, Platelet-Derived Growth Factor alpha genetics, Stomach Diseases pathology
- Abstract
Vanek's tumor (inflammatory fibroid polyp) is a rare benign lesion occurring throughout the digestive tract. Histologically, two patterns can be recognized. Classical Vanek's tumor contains concentric formations of proliferating spindle cells which are CD34 positive. Atypical, inflammatory pseudotumor-like Vanek's tumor lacks concentric formations and is CD34 negative. Recently, mutations in platelet-derived growth factor receptor alpha (PDGFRA) were reported in gastric and small intestinal Vanek's tumors. In this study, KIT exons 9, 11, 13, and 17, PDGFRA exons 12, 14, and 18, and a part of exon 15 BRAF for point mutation V600E were screened in 23 cases of Vanek's tumor, both classical (n = 16) and inflammatory pseudotumor-like (n = 7). No mutations in all analyzed exons of KIT and BRAF and in exon 14 of PDGFRA were detected. Six Vanek's tumors harbored activating mutations in PDGFRA exons 12 (n = 5) and 18 (n = 1) respectively: S566_E571delinsK (n = 1), S566_E571delinsR (n = 4), and D842 del (n = 1). The mutations were detected in the classical (n = 5), as well as inflammatory pseudotumor-like (n = 1) Vanek's tumors. The results of this study suggest that the two morphological patterns of Vanek's tumor more probably represent only variants of one type of tumor than two different lesions. Furthermore, BRAF mutations were not shown to drive growth of PDGFRA wild-type Vanek's tumors.
- Published
- 2010
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- View/download PDF
29. Histologic findings after sodium phosphate bowel preparation for colonoscopy. Diagnostic pitfalls of colonoscopic biopsies.
- Author
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Chlumská A, Benes Z, Mukensnabl P, and Zámecník M
- Subjects
- Administration, Oral, Adult, Aged, Aged, 80 and over, Colon drug effects, Female, Humans, Intestinal Mucosa drug effects, Male, Middle Aged, Young Adult, Cathartics administration & dosage, Colon pathology, Colonoscopy, Intestinal Mucosa pathology, Phosphates administration & dosage
- Abstract
Oral sodium phosphate (NaP) has been increasingly used for bowel preparation before the colonoscopy because it shows good patients tolerance and effective bowel cleansing ability. However, new studies describe that NaP can induce colonic mucosal damage. For better characterization of these changes, we examined histologically segmental colonic biopsies from 42 patients receiving NaP bowel solution before the colonoscopy. The series includes 25 male and 17 female patients in age from 19 to 81 years (average age 46.7 ys). Clinical symptoms in 37 patients included diarrhea, constipation, bleeding and abdominal cramps. The most frequent reason for colonoscopy was suspicion of microscopic colitis. Five patients underwent endoscopy to rule out the presence of neoplasia. None of the patients took drugs before the colonoscopy. Histologically, all specimens showed mild focal edema, hyperemia and hemorrhages. In addition to edema and hemorrhage, in 26 patients (61.9%), patchy mononuclear infiltration in the upper part of lamina propria and increased epithelial cell proliferation of individual crypts were seen. Mucosal structure was normal, with partial sloughing of normal or flattened surface epithelium. In 5 patients (11.9%), some biopsy samples contained scattered neutrophilic leucocytes in the lamina propria/superficial epithelium, isolated basal cryptitis, increased proliferation and apoptosis of the crypt epithelium. In two patients with focal cryptitis (4.8%), small erosions were found. Mild basal cryptitis, increased proliferation and striking apoptosis were present in two inflammatory pseudopolyps (in two patients). In 4 patients, solitary tubular adenomas with low-grade dysplasia without any reactive changes were found. In addition, 300 hyperplastic polyps removed endoscopically after the NaP application, were examined. Two polyps (0.75%) showed cryptitis and isolated multinucleated epithelial cells in the superficial part of the crypts. Our results are similar to those previously described in other studies of colonic changes after the NaP application. It reflects probably a similarity in composition of used NaP solutions.
- Published
- 2010
30. Optical biopsy system distinguishing between hyperplastic and adenomatous polyps in the colon during colonoscopy.
- Author
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Benes Z and Antos Z
- Subjects
- Adenoma diagnosis, Adenoma pathology, Adenomatous Polyposis Coli diagnosis, Adult, Aged, Aged, 80 and over, Biopsy methods, Colonic Neoplasms diagnosis, Colonic Neoplasms pathology, Colonoscopy methods, Diagnosis, Differential, Humans, Hyperplasia diagnosis, Middle Aged, Optics and Photonics methods, Prospective Studies, Adenomatous Polyposis Coli pathology, Colon pathology
- Abstract
Background: It has been established that the removal of adenomatous colon polyps drastically reduces the incidence of colorectal cancer (CRC), but polypectomy is not without risk. The aim was to determine the correlation between the results of an optical biopsy system and the histopathology report of the physical biopsy specimens of the same polyps removed at colonoscopy., Patients and Methods: Paired optical and physical biopsies were performed on 55 polyps with complete polypectomy of the same tissue., Results: Fifty-three adenomatous polyps and two hyperplastic polyps were identified by the hospital pathologist. The optical biopsy system identified 52 polyps as suspect (adenomatous) and 2 as non-suspect (hyperplastic). One villous adenoma could not be optically analyzed due to friability., Conclusion: The WavSTAT Optical Biopsy System provides accurate information to the gastroenterologist to assist in distinguishing between hyperplastic and adenomatous polyps. It is safe for the patient and does not unduly increase the time required for an endoscopic examination.
- Published
- 2009
31. Detection of galectin-3 in patients with inflammatory bowel diseases: new serum marker of active forms of IBD?
- Author
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Frol'ová L, Smetana K Jr, Borovská D, Kitanovicová A, Klimesová K, Janatková I, Malícková K, Lukás M, Drastich P, Benes Z, Tucková L, Manning JC, André S, Gabius HJ, and Tlaskalová-Hogenová H
- Subjects
- Animals, Biomarkers, Blotting, Western, Colitis chemically induced, Colitis, Ulcerative blood, Colon metabolism, Crohn Disease blood, Dextran Sulfate, Electrophoresis, Polyacrylamide Gel, Escherichia coli metabolism, Female, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Humans, Immunohistochemistry, Inflammatory Bowel Diseases chemically induced, Inflammatory Bowel Diseases diagnosis, Lectins metabolism, Lipopolysaccharide Receptors analysis, Lipopolysaccharide Receptors metabolism, Mice, Mice, Inbred BALB C, Galectin 3 blood, Inflammatory Bowel Diseases blood
- Abstract
Objective: It is an open question whether multifunctional galectin-3 can be a serum marker in inflammatory bowel disease., Methods: Western blots and commercial ELISA detected and quantitated the lectin immunocytochemistry using double labeling localized it in tissue sections., Results: Serum concentrations were significantly increased in specimen of patients with active and remission-stage ulcerative colitis and Crohn's disease, associated with emerging positivity of CD14(+) cells., Conclusion: Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.
- Published
- 2009
- Full Text
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32. Anti-calreticulin immunoglobulin A (IgA) antibodies in refractory coeliac disease.
- Author
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Sánchez D, Palová-Jelínková L, Felsberg J, Simsová M, Pekáriková A, Pecharová B, Swoboda I, Mothes T, Mulder CJ, Benes Z, Tlaskalová-Hogenová H, and Tucková L
- Subjects
- Aged, Antibodies, Anti-Idiotypic blood, Blotting, Western, Calreticulin blood, Celiac Disease blood, Celiac Disease diagnosis, Diet, Gluten-Free adverse effects, Enterocytes chemistry, Enterocytes immunology, Enzyme-Linked Immunosorbent Assay, Female, Gliadin blood, Gliadin immunology, Humans, Immunoglobulin A blood, Male, Middle Aged, Sensitivity and Specificity, Transglutaminases blood, Transglutaminases immunology, Antibodies, Anti-Idiotypic immunology, Calreticulin immunology, Celiac Disease immunology, Immunoglobulin A immunology
- Abstract
Refractory coeliac disease (RCD) is a very rare and dangerous form of CD, in which gluten-free diet loses its therapeutic effect and the damage of intestinal mucosa persists. Because of the adherence to the diet, serological markers of CD [immunoglobulin A (IgA) antibodies against gliadin, tissue transglutaminase (tTG) and endomysium] are often missing in RCD patients. We found substantially elevated levels of IgA anti-calreticulin (CRT) antibodies in the sera of almost all RCD patients tested. These sera were negative for IgA antibodies to gliadin and tTG and only some of them showed IgA antibodies to enterocytes. Analysis of patients' IgA reactivity to CRT fragments (quarters and halves) by Western blotting revealed differences in the specificity of IgA antibodies between RCD and CD patients. We therefore used the Pepscan technique with synthetic overlapping decapeptides of CRT to characterize antigenic epitopes recognized by serum IgA antibodies of RCD patients. Employing this method we demonstrated several dominant antigenic epitopes recognized by IgA antibodies of RCD patients on the CRT molecule. Epitope GVTKAAEKQMKD was recognized predominantly by serum IgA of RCD patients. Our results suggest that testing for serum IgA antibodies against CRT and its selected peptide could be a very useful tool in RCD differential diagnosis.
- Published
- 2008
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33. Detection of carcinoma by means of endoscopic cytoscopy in the area of ulcerative colitis.
- Author
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Benes Z, Chlumská A, Antos Z, Kohout P, and Sequens R
- Subjects
- Carcinoma complications, Carcinoma pathology, Colon pathology, Colorectal Neoplasms complications, Colorectal Neoplasms pathology, Humans, Male, Middle Aged, Rectum pathology, Carcinoma diagnosis, Colitis, Ulcerative complications, Colorectal Neoplasms diagnosis, Cystoscopy
- Published
- 2008
- Full Text
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34. Progress in molecular diagnostics of gastrointestinal stromal tumors.
- Author
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Daum O, Vanecek T, Benes Z, and Michal M
- Abstract
Gastrointestinal stromal tumor (GIST) is the most frequent mesenchymal tumor of the alimentary tract, presently being defined as a tumor composed of spindle and/or epithelioid cells presumably differentiating towards interstitial cells of Cajal. The most frequent location of gastrointestinal stromal tumor is the stomach, followed by other sites of gastrointestinal tract. Occasional sites of occurrence are mesenterium, omentum, retroperitoneum, gall bladder, urinary bladder, pancreas, prostate and the vagina. Most of these tumors are KIT-immunoreactive and almost all carry mutated KIT or PDGFRA genes encoding two transmembrane class III tyrosine kinases. These mutations not only shed light on molecular oncogenesis of GISTs, but can also serve as diagnostic markers of this type of tumor, and, last but not least, the function of the proteins encoded by the mutated genes may be influenced by small molecule tyrosine kinase inhibitors. Nevertheless, negative results of immunohistochemistry and mutational analysis do not exclude histologically proven diagnosis of GIST, and although the response to tyrosine kinase inhibitors is correlated with the presence and type of KIT and PDGFRA mutations, the molecular genetic analysis of these genes is presently not required for imatinib therapy of GISTs.
- Published
- 2007
- Full Text
- View/download PDF
35. [Endoscopic cytoscopy in examination of the digestive tract].
- Author
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Benes Z, Daum O, Puskárová G, Kohout P, Antos Z, and Cerník M
- Subjects
- Endoscopes, Gastrointestinal, Humans, Intestinal Mucosa pathology, Endoscopy, Gastrointestinal
- Abstract
A number of new endoscopic methods have been developed recently, which aim to allow the most accurate possible viewing of the mucosa of the digestive tract. These procedures include endoscopic cytoscopy, which together with confocal endoscopy is a technique of so-called endoscopic microscopy. By means of enodcytoscopy it is possible to view "in vivo" the morphological details of the surface of the mucosa of the digestive tract. The mucosa must, however, be thoroughly cleaned and stained with methylene blue. The paper presents our own initial experience of this method, which may lead to faster and more accurate diagnosis of pre-tumorous or tumourous inflammation processes in the mucosa of the digestive tract.
- Published
- 2007
36. [Pseudomembranous colitis].
- Author
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Bergmann D, Koten J, Benes Z, Kohout P, and Chlumská A
- Subjects
- Humans, Enterocolitis, Pseudomembranous diagnosis, Enterocolitis, Pseudomembranous microbiology, Enterocolitis, Pseudomembranous therapy
- Abstract
For some thirty years it has been known that pathologic proliferation oftoxigenic Clostridium difficile may lead to inflammation of colonic mucosa which, in its fully developed form, manifests as pseudomembranous colitis. CDAD (Clostridium difficile - Associated Disease or Diarrhoea) is the term which is generally and quite aptly used for the disease in literature on the subject. In most cases, the disease develops after the administration of broad-spectrum antibiotics, yet there are also other important pathogenetic factors involved which should not be ignored. The disease has grown in significance over recent years due to the occurrence of serious epidemics in a number of advanced countries provoked by a highly virulent strain characterised as ribotype 027, toxinotype III, pulsovar NAP1. CDAD is a potentially fatal disease, yet it can be effectively cured if diagnosed on time. Stool toxin testing plays a crucial role in the diagnostics of the disease, and the use of endoscopy has been on the rise. Administration of targeted antibiotics is of critical importance for successful therapy, metronidazol or orally administered vankomycine being considered the most reliable, and there are also other drugs with promising effects. However, the rate of recurrence of the disease is as high as 25%. In view of the realistic threat of propagation of the highly virulent strain in the territory of the Czech Republic, preparations have started for the setting up of a national reference laboratory for Clostridium difficile typification.
- Published
- 2007
37. Histopathologic changes in gastroesophageal reflux disease. A study of 126 bioptic and autoptic cases.
- Author
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Chlumská A, Boudová L, Benes Z, and Zámecník M
- Subjects
- Adult, Cardia pathology, Esophagogastric Junction pathology, Esophagus pathology, Female, Gastroesophageal Reflux diagnosis, Humans, Male, Middle Aged, Gastroesophageal Reflux pathology
- Abstract
The histologic diagnosis of reflux esophagitis is still complicated by the lack of a consensus opinion on what is the normal mucosa in the area of the gastroesophageal junction (GEJ). Most authors consider GEJ as the junction between the squamous and the cardiac epithelium. The cardiac mucosa is composed of mucinous or mixed mucinous-oxyntic glands. These glands are in fact indistinguishable from metaplastic mucosa that arises in the distal esophagus in consequence of gastroesophageal reflux (GER). The cardiac mucosa shows invariably chronic inflammatory changes referred to as "carditis". The cause of "carditis" is GER and/or Helicobacter pylori (HP) infection. In our series of 120 endoscopic biopsies of the GEJ and distal esophagus the cardia type mucosa (CM) was always present. In 15 cases, it was accompanied by oxyntocardiac mucosa. Both mucosa types showed chronic inflammation that is after exclusion of HP infection regarded as a strong diagnostic sign of the gastroesophageal reflux disease (GERD). In two cases with clinical symptoms of GERD, a few HP were found on the CM. Therefore we diagnosed them as GERD with secondary HP infection. In 17 cases, CM displayed intestinal metaplasia (IM) predominantly of incomplete type and no dysplasia. This IM expressed MUC6 in the glandular zone of the mucosa like it did in the neighboring glands, whereas in the surface and foveolar epithelium the MUC6 was negative or only slightly and focally positive. On the other hand, IM in the surface and foveolar epithelium was reactive for MUC5AC. The positivity and distribution of CK7 and CK20 was very similar in the Barrett's mucosa, cardiac mucosa and antral mucosa. In one specimen of esophagus resected for adenocarcinoma, CM with incomplete IM was found in the vicinity of the tumor. Squamous metaplastic epithelium was often seen near the orifices of submucosal esophageal glands in these areas, indicating the metaplastic nature of the glandular mucosa in the distal esophagus. In the GEJ of 5 autopsy cases of children with spastic quadriplegia (age range 7-10 years) CM in a short segment (0.5-3 mm in length), probably of metaplastic origin was identified, showing chronic inactive inflammation.
- Published
- 2007
38. [The history and current potential of diagnostic laparoscopy].
- Author
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Koten J and Benes Z
- Subjects
- Humans, Laparoscopy
- Published
- 2007
39. [Complications of laparoscopic procedures of inguinal hernias, diagnosed on colonoscopy].
- Author
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Benes Z, Herdegen P, Puskárová G, and Antos Z
- Subjects
- Aged, Device Removal, Female, Humans, Male, Reoperation, Surgical Mesh adverse effects, Colonoscopy, Hernia, Inguinal surgery, Laparoscopy adverse effects, Postoperative Complications diagnosis
- Abstract
Aim of the Study: The aim was to assess significance of colonoscopy in detection of inguinal hernias - dislocation of the mesh into the lumen of the large intestine, following inguinal hernia laparoscopic procedures (TAPP)., Material, Methodology and Results: From 01-01-2004 to 31-12-2006, the authors performed 3 colonoscopies in 3 subjects after TAPP inguinal hernia laparoscopic procedures. The subjects included 2 males and 1 female, 4 to 9 months after the laparoscopic procedure, colonoscopy was performed, which was indicated for the lower abdominal pain and enterorrhagia. However, no signs of a relapsing hernia were recorded. Endoscopy detected irritation and bleeding of the sigmoid, and a mesh, which passed through the intestinal wall was detec ted in the sigmoid lumen. The subjects were then reoperated and the mesh was removed. In one subject, resection of the sigmoid had to be performed as well. The subject recovered., Conclusion: The postoperative complications of the TAPP procedure included dislocation of the mesh into the intestinal lumen. Detection of the mesh dislocation into the lumen of the large intestine facilitates indication for surgical management.
- Published
- 2007
40. [Imported cryptococcal infection mimicking primary sclerosing cholangitis].
- Author
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Benes Z, Koten J, Kabelková M, and Benes J
- Subjects
- Adult, Cryptococcosis epidemiology, Czech Republic epidemiology, Diagnosis, Differential, Egypt, Humans, Male, Cholangitis, Sclerosing diagnosis, Cryptococcosis diagnosis, Travel
- Abstract
Expanding travel industry may increase the incidence of some rare infections diseases. One of them is cryptococcosis developing especially in immunosuppressed individuals. However, massive cryptococcal infection may lead to a severe clinical course even in people without impaired immunity. The case report describes the disease in a non-immunosuppressed 26-year-old man who returned to the Czech Republic after a 2.5-month stay in Egypt. He was hospitalized for increasing fever, weight loss and changes in behaviour. Thorough examination including thoracoscopy and histological analysis of the mediastinal lymph nodes revealed cryptococcal disease. Various organs, mainly the small bile ducts, were affected by the dissemination mimicking primary sclerosing cholangitis. The discussion is concerned with the properties of the Cryptococcus, variety of clinical manifestations, differential diagnosis and treatment.
- Published
- 2006
41. Autoimmune gastritis. A clinicopathologic study of 25 cases.
- Author
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Chlumská A, Boudová L, Benes Z, and Zámecník M
- Subjects
- Aged, Autoimmune Diseases metabolism, Female, Gastric Mucosa metabolism, Gastric Mucosa pathology, Gastritis, Atrophic metabolism, Humans, Immunohistochemistry, Male, Middle Aged, Autoimmune Diseases pathology, Gastritis, Atrophic pathology
- Abstract
The histopathological diagnosis of autoimmune gastritis (AG) in its early stages can be a diagnostic challenge. Even some advanced cases with complete atrophy of the corpus mucosa may be difficult to recognize. To establish the diagnosis of autoimmune gastritis, several histological features should be assessed and combined with immunostains for enterochromaffin cell-like (ECL) cells and G-cells. The main histological criteria include a mononuclear infiltrate within the lamina propria, foci of destruction of oxyntic glands, intestinal metaplasia (IM), pyloric metaplasia, and parietal cell pseudohypertrophy. These criteria were evaluated in our series of 25 patients with achlorhydria and/or megaloblastic anemia. Some of our patients presented with nonspecific gastrointestinal symptoms. The age ranged between 46 and 79 years; one male patient was only 31 years old. Histologically, the corpus mucosa displayed in all cases chronic inflammation with focal complete IM and advanced pyloric metaplasia. In 4 patients, oxyntic glands were destructed in some sites. There was a pancreatic metaplasia of acinar type in 2 patients and a minimal focal pseudohypertrophy of parietal cells in the 31-year-old man. A tubular adenoma with a low-grade dysplasia was found in one female patient. Immunohistochemically, chromogranin-A highlighted linear or nodular hyperplasia of ECL cells in 19 patients, and adenomatoid ECL hyperplasia in one case (80%). In the remaining cases hyperplasia of ECL cells could not be recognized from their normal count. In 13 cases (52%) a few ECL cells were seen also in IM. Regarding associated pathology, in one woman with nodular ECL cell hyperplasia, a gastric carcinoid was removed endoscopically. The reaction with gastrin antibody revealed in 11 cases (44%) a small number of G cells in IM in the corpus mucosa. In 18 patients, antral mucosa was examined as well. In 8 patients, the mucosa was normal; in 10 cases, a mild chronic inactive gastritis was diagnosed, and in 15 patients G-cell hyperplasia was found. In accordance with other studies, we show that the diagnosis of AG may be established microscopically in endoscopic specimens of the gastric body mucosa when histologic features and immunohistochemical detection of ECL and G cell hyperplasia are combined.
- Published
- 2005
42. Pigmented solid-pseudopapillary neoplasm of the pancreas.
- Author
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Daum O, Sima R, Mukensnabl P, Vanecek T, Brouckova M, Benes Z, and Michal M
- Subjects
- CD56 Antigen analysis, Carcinoma, Papillary genetics, Carcinoma, Papillary metabolism, Cytoskeletal Proteins genetics, Female, Humans, Immunohistochemistry, Microscopy, Electron, Middle Aged, Mutation, Missense, Pancreas metabolism, Pancreas pathology, Pancreas ultrastructure, Pancreatic Neoplasms genetics, Pancreatic Neoplasms metabolism, Phosphopyruvate Hydratase analysis, Trans-Activators genetics, Vimentin analysis, beta Catenin, Carcinoma, Papillary pathology, Pancreatic Neoplasms pathology
- Abstract
A hitherto unrecognized variant of solid-pseudopapillary neoplasm of the pancreas is reported. The tumor presented in the pancreatic head of a 57-year-old female patient. It was a well-circumscribed, encapsulated nodule measuring 27 mm in diameter, with variegated yellow to brown and gray cut surface. Histologically, the neoplasm was composed of uniform polyhedral cells arranged around delicate fibrovascular cores retaining their solid pattern in the periphery, whereas central parts of the tumor were characterized by the formation of papillae and smaller pseudocysts. Neither mitotic activity nor invasive growth were found. Immunohistochemically, tumor cells were positive for vimentin, neuron-specific enolase, and CD56, whereas they were negative in reactions with antibodies directed against other neuroendocrine markers, cytokeratins, melanocytic markers, and pancreatic amylase. In addition to these typical findings, intracellular pigmented granules were found in the darker brown zones of the tumor. They were positively stained in periodic acid-Schiff reaction after diastase digestion, sudan black B, and in Schmorl stain. In contrast, they were not stained with Fontana-Masson, Ziehl-Neelsen, and Perls stains. Ultrastructurally, the pigment consisted of dense granules with lipid droplets resembling modified lysosomes. These results exclude the possibility of a melanogenic nature of the pigment and instead determine it as lipofuscin.
- Published
- 2005
- Full Text
- View/download PDF
43. [Crohn's disease and infectious agents].
- Author
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Benes Z
- Subjects
- Bacteria isolation & purification, Bacterial Infections complications, Crohn Disease diagnosis, Crohn Disease physiopathology, Crohn Disease therapy, Humans, Intestines microbiology, Crohn Disease microbiology
- Abstract
The author discusses possible etiological causes of Crohn's disease taking into consideration the undoubtedly significant impact of the constitution of the intestinal microbial flora. The author pays special attention to Yersinia; their presence in the intestines is believed to be the chief mechanism of subsequent immunity events resulting in the development of the disease. The author briefly discusses the possibilities of differential diagnosis and of treatment. He mentions the profitable cooperation of infectiousdiseases specialists and gastroenterologists in the diagnosis of diarrhoeal disorders.
- Published
- 2005
44. [Ulcerative colitis--contemporary morphological criteria].
- Author
-
Chlumská A, Benes Z, and Mukensnabl P
- Subjects
- Adolescent, Adult, Aged, Biopsy, Female, Humans, Intestinal Mucosa pathology, Intestine, Large pathology, Male, Middle Aged, Colitis, Ulcerative pathology
- Abstract
Regular bioptical examinations of patients with ulcerative colitis (UC) performed in recent years show that the inflammatory changes of the mucosa of the large intestine are not necessarily diffuse, and that their extent may vary in the course of the disease. To establish the diagnosis of UC and to assess the treatment efficacy it is important to examine histologically multiple mucosal specimens from different levels of the large intestine. In our series of 27 patients with ulcerative colitis (18 men and 9 women at the age of 17 to 76 years), active or active and inactive pancolitis was diagnosed in 25 cases (93%). In 11 of these, the whole of the large intestine was affected. Two patients showed diffuse pancolitis without caecal involvement, in 5 cases there was inactive inflammation in the rectum or in the sigmoid colon. Seven patients had active colitis of the rectum and sigmoid. In another 2 patients (7%), the inflammation was limited to several segments of the large intestine only (the descending colon, and the descending and transverse colon). On bioptical examination of 6 patients repeated after 2-29 months (mean 14 months), there were changes in the distribution and appearance of the inflammation. Thus our findings correspond with the results of previous studies: UC does not always affect the mucosa of the large intestine diffusely. Further, the extent and distribution of inflammatory changes vary in the course of the disease.
- Published
- 2004
45. Reactive nodular fibrous pseudotumors of the gastrointestinal tract: report of 8 cases.
- Author
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Daum O, Vanecek T, Sima R, Curik R, Zamecnik M, Yamanaka S, Mukensnabl P, Benes Z, and Michal M
- Subjects
- Adult, Aged, Biomarkers analysis, Female, Fibrosis metabolism, Fibrosis surgery, Gastrointestinal Diseases metabolism, Gastrointestinal Diseases surgery, Humans, Immunohistochemistry, Infant, Male, Middle Aged, Treatment Outcome, Fibrosis pathology, Gastrointestinal Diseases pathology
- Abstract
Eight cases of reactive nodular fibrous pseudotumor of the gastrointestinal tract are presented. The patients included 6 males and 2 females between the ages of 1 and 68 years (mean age 41.5 years). Three tumors involved the small intestine, and 5 of the investigated lesions were located in the large bowel. Of these, 2 originated in the sigmoid colon, 1 in the cecum, 1 in the appendix, and 1 in the large bowel not otherwise specified. The tumors' size varied from 3 to 10 cm in the greatest diameter (mean 6.2 cm). Histologically they were composed of stellate or spindle shaped cells resembling fibroblasts arranged haphazardly or in intersecting fascicles, embedded in a collagen-rich stroma, with sparse intralesional mononuclear cells frequently arranged in lymphoid aggregates. Immunohistochemically, the lesions were positive for vimentin (7/7), smooth muscle actin (8/8), muscle-specific actin (5/7), cytokeratins AE1/AE3 (6/7), and CAM 5.2 (1/7), and antigen CD68 (1/7). No case (0/8) reacted positively with antibody to CD117 (c-kit). Genetically no substitutions, deletions, or insertions occurred in exon 11 in all analyzed samples. Likewise, no deletions or insertions in part of exon 9 were observed. Ultrastructurally the tumor cells revealed features typical of myofibroblasts. According to the morphologic, immunohistochemical, and ultrastructural features mentioned above, especially to the positivity of low-molecular-weight cytokeratins, we propose this lesion to be related to a proliferation of multipotential subserosal cells rather than ordinary myofibroblasts or fibroblasts.
- Published
- 2004
- Full Text
- View/download PDF
46. Epitopes of calreticulin recognised by IgA autoantibodies from patients with hepatic and coeliac disease.
- Author
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Sánchez D, Tucková L, Mothes T, Kreisel W, Benes Z, and Tlaskalová-Hogenová H
- Subjects
- Adolescent, Adult, Aged, Amino Acid Sequence, Autoantibodies blood, Celiac Disease blood, Epitopes chemistry, GTP-Binding Proteins immunology, Gliadin immunology, Humans, Immunoglobulin A blood, Immunoglobulin G blood, Immunoglobulin G immunology, Liver Diseases blood, Middle Aged, Molecular Sequence Data, Protein Glutamine gamma Glutamyltransferase 2, Recombinant Proteins, Transglutaminases immunology, Autoantibodies immunology, Calreticulin chemistry, Calreticulin immunology, Celiac Disease immunology, Epitopes immunology, Immunoglobulin A immunology, Liver Diseases immunology
- Abstract
Calreticulin (CRT) was identified as a frequent target of serum autoantibodies (Ab) in various diseases, but anti-CRT Ab of IgA isotype were described only in coeliac (CLD) and some hepatic diseases. Employing ELISA with recombinant CRT we found significantly higher (P<0.001) levels of IgA anti-CRT Ab in sera of patients with primary biliary cirrhosis (PBC) (77.6+/-8.9 AU/mean+/-SE), autoimmune hepatitis (AIH) (105.1+/-9.2 AU) and alcoholic liver cirrhosis (ALC) (193.5+/-21.0 AU) relative to healthy controls (38.6+/-2.0 AU). The levels of IgG anti-CRT Ab in sera of patients with PBC (59.5+/-3.4 AU), AIH (89.7+/-7.9 AU) and ALC (86.4+/-6.2 AU) were also significantly increased (P<0.001) when compared with controls (38.5+/-2.1 AU). Pepscan technique with decapeptides of CRT (each overlapping by eight amino acids) revealed antigenic epitopes of this molecule recognised by IgA Ab of almost all tested patients-KGKNVLINKD and QVKSGTIFDNFL. We also identified disease specific antigenic epitopes on CRT molecule, predominantly recognised by IgA Ab of patients suffering from a particular disease: GGYVKLFPNS and YVKLFPNSLD in AIH (83%, 92% of patients), GLQTSQDARF and EQRLKEEEED in CLD (both 75%) and ASKPEDWDER in ALC (67%). Identification of disease specific CRT epitopes contributes to clarification of autoreactivity against this molecule.
- Published
- 2003
- Full Text
- View/download PDF
47. Vanek's tumor (inflammatory fibroid polyp). Report of 18 cases and comparison with three cases of original Vanek's series.
- Author
-
Daum O, Hes O, Vanecek T, Benes Z, Sima R, Zamecnik M, Mukensnabl P, Hadravska S, Curik R, and Michal M
- Subjects
- Aged, Aged, 80 and over, Antigens, CD34 analysis, Biomarkers, Tumor analysis, Diagnosis, Differential, Female, Fibroma chemistry, Gastroenteritis diagnosis, Granuloma, Plasma Cell diagnosis, Hemangioendothelioma diagnosis, Hemangiopericytoma diagnosis, Humans, Intestinal Neoplasms chemistry, Intestinal Polyps chemistry, Male, Middle Aged, Stomach Neoplasms chemistry, Stromal Cells pathology, Vimentin analysis, Fibroma pathology, Intestinal Neoplasms pathology, Intestinal Polyps pathology, Stomach Neoplasms pathology
- Abstract
Eighteen cases of Vanek's tumors are presented. The patients included nine men and nine women between the ages of 45 and 93 years (mean, 66.2 years). Nine cases were clinically diagnosed as polyps of the gastric antrum, five cases as polyps of the stomach (not otherwise specified), one polyp was located in the ileum and the three remaining polyps in the small intestine (not otherwise specified). The thirteen polyps with available size information measured from 0.4 to 5 cm in the greatest diameter (mean, 2.2 cm). Immunohistochemically, the affections were positive for vimentin (18/18) and CD34 (15/18). All the cases negative for CD34 also lacked concentric onion skin-like formations of the spindle cells around glands and vessels. The different immunophenotype and absence of concentric formations could be explained by the existence of two different lesions commonly designated as Vanek's tumor (inflammatory fibroid polyp) or by the hypothesis of various evolutional stages. In the differential diagnosis, it is important to distinguish namely eosinophilic gastroenteritis, gastrointestinal stromal tumor, inflammatory pseudotumor, hemangioendothelioma, and hemangiopericytoma. In contrast to gastrointestinal stromal tumors, genetically no substitution, deletion, or insertion occurred in c-kit exon 11 in all analyzed samples. Likewise, no deletion or insertion in part of c-kit exon 9 was observed.
- Published
- 2003
- Full Text
- View/download PDF
48. Regarding the paper by Vieth et al. Virchows Archiv 442/4:317-321.
- Author
-
Michal M, Curik R, Matler K, and Benes Z
- Subjects
- Female, Humans, Middle Aged, Adenoma pathology, Gastric Mucosa pathology, Stomach Neoplasms pathology
- Published
- 2003
- Full Text
- View/download PDF
49. Phonon density of states of single-wall carbon nanotubes
- Author
-
Rols S, Benes Z, Anglaret E, Sauvajol JL, Papanek P, Fischer JE, Coddens G, Schober H, and Dianoux AJ
- Abstract
The vibrational density of states of single-wall carbon nanotubes (SWNT) was obtained from inelastic neutron scattering data from 0 to 225 meV. The spectrum is similar to that of graphite above 40 meV, while intratube features are clearly observed at 22 and 36 meV. An unusual energy dependence below 10 meV is assigned to contributions from intertube modes in the 2D triangular lattice of SWNT bundles, and from intertube coupling to intratube excitations. Good agreement between experiment and a calculated density of states for the SWNT lattice is found over the entire energy range.
- Published
- 2000
- Full Text
- View/download PDF
50. Occurrence of IgA and IgG autoantibodies to calreticulin in coeliac disease and various autoimmune diseases.
- Author
-
Sánchez D, Tucková L, Sebo P, Michalak M, Whelan A, Sterzl I, Jelínková L, Havrdová E, Imramovská M, Benes Z, Krupicková S, and Tlaskalová-Hogenová H
- Subjects
- Adolescent, Adult, Aged, Blotting, Western, Calreticulin, Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Humans, Middle Aged, Autoantibodies blood, Autoimmune Diseases immunology, Calcium-Binding Proteins immunology, Celiac Disease immunology, Immunoglobulin A blood, Immunoglobulin G blood, Ribonucleoproteins immunology
- Abstract
Calreticulin (CRT), a high-affintiy calcium binding protein and chaperone, was recently identified as one of the targets of autoantibodies in coeliac disease. We evaluated the level of IgA and IgG antibodies to CRT in sera from patients with coeliac disease and various autoimmune diseases. The level of antibodies to gliadin (shown previously to cross-react with CTR), isolated enterocytes and tissue transglutaminase were determined for comparison. The mean level of IgA antibodies to CRT was significantly higher (P< 0.001) in sera from coeliac patients with active disease (139.9+/-11.2 AU/+/-SE) than in healthy controls (20.9+/-1.7 AU). In sera of patients with systemic lupus erythematosus (SLE), insulin dependent diabetes mellitus (IDDM), multiple sclerosis (MS) and autoimmune thyroiditis (AT) or inflammatory bowel disease (IBD) the mean level (25.8+/-3.7 to 38.1+/-5.6 AU) did not exceed the cut-off value. A low level of these antibodies, however, was detected in some sera of patients with MS and IBD. The level of IgG anti-CRT antibodies was increased in coeliac patients (mean 125.4+/-8.0 AU, P< 0.001) when compared to that in healthy controls (33.9+/-2.3 AU). The IgG anti-CRT antibodies were also detected in about 30% of SLE patients sera (54.1+/-3.6 AU, P< 0.001), but the mean level reached only half that detected in coeliac patients., (Copyright 2000 Academic Press.)
- Published
- 2000
- Full Text
- View/download PDF
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