14 results on '"Baro, Barbara"'
Search Results
2. High-titre methylene blue-treated convalescent plasma as an early treatment for outpatients with COVID-19: a randomised, placebo-controlled trial
- Author
-
Ferrer, Susana, Gallardo, Mireia, Ubals, Maria, González-Beiras, Camila, Vall-Mayans, Martí, Suñer, Clara, Laporte-Villar, Clàudia, Nieto, Aroa, Comas-Leon, Xavier, Jiménez, Zahida, Ramírez-Viaplana, Ferran, Delgado-Capel, Maria, Díez Sánchez, Beatriz, Pons Barber, Maria, Gonzalez Ruiz, Cristian, Navarrete Gonzalez, Laura, González García, David, Vivero Larraza, Ainhoa, Carceles Peiró, Victor, Roquer López, Clàudia, Robert, Neus, Palet, Carles, Gudiol, Carlota, Casares Gonzalez, Pablo, Arcos Vila, Gemma, Flores Aguilera, Begoña, Rodríguez-Sevilla, Graciela, Dastis Arias, Macarena, Roca Font, Judit, Carrasco Matos, Katherine M., Saüch Valmaña, Glòria, Vidal Obradors, Carla, Tarres García, Silvia, Curriu Sabatès, Margarida, Nieto Rodríguez, Raquel, Línio, Rosa, Fornos, Míriam, Casamitjana, Natàlia, Alonso, Eva, Martínez, Núria, Maglio, Laura Analía, Comellas Fernandez, Laura, Garcia, Nadia, Hernández, Luis, González, Maria Isabel, Bravo, Anna, García, Yolanda, Sauleda Oliveras, Silvia, Vertiz, Tatiana, Benavent, Sergio, Bianco, Andrea Sofia, Verdaguer, Joaquim, Briones Zambrano, Ney Nicanor, Viozquez Meya, Maria, Hernández, Águeda, Casaña Lopez, Cristina, Bordoy, Antoni E., González Soler, Victoria, Giménez, Montserrat, París, Alexa, Marfil, Silvia, Trinité, Benjamin, Grau, Eulàlia, Alemany, Andrea, Millat-Martinez, Pere, Corbacho-Monné, Marc, Malchair, Pierre, Ouchi, Dan, Ruiz-Comellas, Anna, Ramírez-Morros, Anna, Rodríguez Codina, Joana, Amado Simon, Rosa, Videla, Sebastian, Costes, Gèlia, Capdevila-Jáuregui, Mar, Torrano-Soler, Pamela, San José, Alba, Bonet Papell, Glòria, Puig, Jordi, Otero, Aurema, Ruibal Suarez, Jose Carlos, Zarauza Pellejero, Alvaro, Llopis Roca, Ferran, Rodriguez Cortez, Orlando, Garcia Garcia, Vanesa, Vidal-Alaball, Josep, Millan, Anna, Contreras, Enric, Grifols, Joan-Ramon, Ancochea, Àgueda, Galvan-Femenia, Ivan, Piccolo Ferreira, Francini, Bonet, Mireia, Cantoni, Jordi, Prat, Núria, Ara, Jordi, Forcada Arcarons, Anna, Farré, Magí, Pradenas, Edwards, Blanco, Julià, Àngel Rodriguez-Arias, Miquel, Fernández Rivas, Gema, Marks, Michael, Bassat, Quique, Blanco, Ignacio, Baro, Bàrbara, Clotet, Bonaventura, and Mitjà, Oriol
- Published
- 2022
- Full Text
- View/download PDF
3. Transmission of COVID-19 in 282 clusters in Catalonia, Spain: a cohort study
- Author
-
Marks, Michael, Millat-Martinez, Pere, Ouchi, Dan, Roberts, Chrissy h, Alemany, Andrea, Corbacho-Monné, Marc, Ubals, Maria, Tobias, Aurelio, Tebé, Cristian, Ballana, Ester, Bassat, Quique, Baro, Bàrbara, Vall-Mayans, Martí, G-Beiras, Camila, Prat, Nuria, Ara, Jordi, Clotet, Bonaventura, and Mitjà, Oriol
- Published
- 2021
- Full Text
- View/download PDF
4. Quantitative assessment of C-polysaccharide in capsular polysaccharides of Streptococcus pneumoniae by 31PNMR
- Author
-
Garrido, Raine, Baró, Bárbara, Soubal, Jean Pierre, and Santana, Darielys
- Published
- 2021
- Full Text
- View/download PDF
5. Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence
- Author
-
Toda, Haruka, Diaz-Varela, Miriam, Segui-Barber, Joan, Roobsoong, Wanlapa, Baro, Barbara, Garcia-Silva, Susana, Galiano, Alicia, Gualdrón-López, Melisa, Almeida, Anne C. G., Brito, Marcelo A. M., de Melo, Gisely Cardoso, Aparici-Herraiz, Iris, Castro-Cavadía, Carlos, Monteiro, Wuelton Marcelo, Borràs, Eva, Sabidó, Eduard, Almeida, Igor C., Chojnacki, Jakub, Martinez-Picado, Javier, Calvo, Maria, Armengol, Pilar, Carmona-Fonseca, Jaime, Yasnot, Maria Fernanda, Lauzurica, Ricardo, Marcilla, Antonio, Peinado, Hector, Galinski, Mary R., Lacerda, Marcus V. G., Sattabongkot, Jetsumon, Fernandez-Becerra, Carmen, and del Portillo, Hernando A.
- Published
- 2020
- Full Text
- View/download PDF
6. Low transmission of SARS-CoV-2 derived from children in family clusters: An observational study of family households in the Barcelona Metropolitan Area, Spain.
- Author
-
Mele-Casas, Maria, Launes, Cristian, de Sevilla, Mariona F., Hernandez-Garcia, Maria, Pons-Tomas, Gemma, Bassat, Quique, Fumado, Victoria, Fortuny, Claudia, Garcia-Miquel, Aleix, Bonet-Carne, Elisenda, Prats, Clara, Ajanovic, Sara, Cubells, Marta, Claverol, Joana, Penela-Sanchez, Daniel, Jou, Cristina, Arias, Sara, Balanza, Nuria, Baro, Barbara, and Millat-Martinez, Pere
- Subjects
METROPOLITAN areas ,HOUSEHOLDS ,SARS-CoV-2 ,VIRAL transmission ,SCIENTIFIC observation ,SCHOOL children - Abstract
Background: Family clusters offer a good opportunity to study viral transmission in a stable setting. We aimed to analyze the specific role of children in transmission of SARS-CoV-2 within households. Methods: A prospective, longitudinal, observational study, including children with documented acute SARS-CoV-2 infection attending 22 summer-schools in Barcelona, Spain, was performed. Moreover, other patients and families coming from other school-like environments that voluntarily accessed the study were also studied. A longitudinal follow-up (5 weeks) of the family clusters was conducted to determine whether the children considered to be primary cases were able to transmit the virus to other family members. The household reproduction number (Re*) and the secondary attack rate (SAR) were calculated. Results: 1905 children from the summer schools were screened for SARS-CoV-2 infection and 22 (1.15%) tested positive. Moreover, 32 additional children accessed the study voluntarily. Of these, 37 children and their 26 households were studied completely. In half of the cases (13/26), the primary case was considered to be a child and secondary transmission to other members of the household was observed in 3/13, with a SAR of 14.2% and a Re* of 0.46. Conversely, the SAR of adult primary cases was 72.2% including the kids that gave rise to the contact tracing study, and 61.5% without them, and the estimated Re* was 2.6. In 4/13 of the paediatric primary cases (30.0%), nasopharyngeal PCR was persistently positive > 1 week after diagnosis, and 3/4 of these children infected another family member (p<0.01). Conclusions: Children may not be the main drivers of the infection in household transmission clusters in the study population. A prolonged positive PCR could be associated with higher transmissibility. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
7. Analytical and clinical performance of the panbio COVID-19 antigen-detecting rapid diagnostic test
- Author
-
Alemany, Andrea, Baró, Bàrbara, Ouchi, Dan, Rodó, Pau, Ubals, Maria, Corbacho-Monné, Marc, Vergara-Alert, Júlia, Rodon, Jordi, Segalés, Joaquim, Esteban, Cristina, Fernández, Gema, Ruiz, Lidia, Bassat, Quique, Clotet, Bonaventura, Ara, Jordi, Vall-Mayans, Martí, G-Beiras, Camila, Blanco, Ignacio, and Mitjà, Oriol
- Published
- 2021
- Full Text
- View/download PDF
8. Multiplex Antibody Analysis of IgM, IgA and IgG to SARS-CoV-2 in Saliva and Serum From Infected Children and Their Close Contacts.
- Author
-
Dobaño, Carlota, Alonso, Selena, Vidal, Marta, Jiménez, Alfons, Rubio, Rocío, Santano, Rebeca, Barrios, Diana, Pons Tomas, Gemma, Melé Casas, María, Hernández García, María, Girona-Alarcón, Mònica, Puyol, Laura, Baro, Barbara, Millat-Martínez, Pere, Ajanovic, Sara, Balanza, Núria, Arias, Sara, Rodrigo Melero, Natalia, Carolis, Carlo, and García-Miquel, Aleix
- Subjects
SALIVA ,IMMUNOGLOBULIN G ,IMMUNOGLOBULIN A ,SARS-CoV-2 ,CHILD patients - Abstract
COVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 to their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed to evaluate the infectivity dynamics of SARS-CoV-2 in pediatric populations and guide public health interventions, particularly if this population is not fully vaccinated. We evaluated the utility of high-throughput Luminex assays to quantify saliva IgM, IgA and IgG antibodies against five SARS-CoV-2 spike (S) and nucleocapsid (N) antigens in a contacts and infectivity longitudinal study in 122 individuals (52 children and 70 adults). We compared saliva versus serum/plasma samples in infected children and adults diagnosed by weekly RT-PCR over 35 days (n=62), and those who consistently tested negative over the same follow up period (n=60), in the Summer of 2020 in Barcelona, Spain. Saliva antibody levels in SARS-CoV-2 RT-PCR positive individuals were significantly higher than in negative individuals and correlated with those measured in sera/plasmas. Asymptomatic infected individuals had higher levels of anti-S IgG than symptomatic individuals, suggesting a protective anti-disease role for antibodies. Higher anti-S IgG and IgM levels in serum/plasma and saliva, respectively, in infected children compared to infected adults could also be related to stronger clinical immunity in them. Among infected children, males had higher levels of saliva IgG to N and RBD than females. Despite overall correlation, individual clustering analysis suggested that responses that may not be detected in blood could be patent in saliva, and vice versa. In conclusion, measurement of SARS-CoV-2-specific saliva antibodies should be considered as a complementary non-invasive assay to serum/plasma to determine COVID-19 prevalence and transmission in pediatric populations before and after vaccination campaigns. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
9. Plasmodium vivax gametocytes in the bone marrow of an acute malaria patient and changes in the erythroid miRNA profile.
- Author
-
Baro, Barbara, Deroost, Katrien, Raiol, Tainá, Brito, Marcelo, Almeida, Anne C. G., de Menezes-Neto, Armando, Figueiredo, Erick F. G., Alencar, Aline, Leitão, Rodrigo, Val, Fernando, Monteiro, Wuelton, Oliveira, Anna, Armengol, Maria del Pilar, Fernández-Becerra, Carmen, Lacerda, Marcus V., and del Portillo, Hernando A.
- Subjects
- *
PLASMODIUM vivax , *BONE marrow , *MALARIA , *BLOOD sampling , *MICRORNA , *ERYTHROPOIESIS , *PATIENTS - Abstract
The article discusses the presence of plasmodium (P.) vivax gametocytes in the bone marrow of patient with acute malaria. It presents a morphological and molecular study of bone marrow aspirates and peripheral blood samples taken from patient who was diagnosed with P. vivax infection. It also provides information on the role of micro (mi)RNAs in controlling erythropoiesis during P. vivax infection.
- Published
- 2017
- Full Text
- View/download PDF
10. Dual Regulation of the Mitotic Exit Network (MEN) by PP2A-Cdc55 Phosphatase.
- Author
-
Baro, Barbara, Rodriguez-Rodriguez, Jose-Antonio, Calabria, Ines, Hernáez, María Luisa, Gil, Concha, and Queralt, Ethel
- Subjects
- *
CELL cycle , *PHOSPHATASES , *ACID phosphatase , *PHOSPHORYLATION , *CHEMICAL reactions - Abstract
Exit from mitosis in budding yeast is triggered by activation of the key mitotic phosphatase Cdc14. At anaphase onset, the protease separase and Zds1 promote the downregulation of PP2ACdc55 phosphatase, which facilitates Cdk1-dependent phosphorylation of Net1 and provides the first wave of Cdc14 activity. Once Cdk1 activity starts to decline, the mitotic exit network (MEN) is activated to achieve full Cdc14 activation. Here we describe how the PP2ACdc55 phosphatase could act as a functional link between FEAR and MEN due to its action on Bfa1 and Mob1. We demonstrate that PP2ACdc55 regulates MEN activation by facilitating Cdc5- and Cdk1-dependent phosphorylation of Bfa1 and Mob1, respectively. Downregulation of PP2ACdc55 initiates MEN activity up to Cdc15 by Bfa1 inactivation. Surprisingly, the premature Bfa1 inactivation observed does not entail premature MEN activation, since an additional Cdk1-Clb2 inhibitory signal acting towards Dbf2-Mob1 activity restrains MEN activity until anaphase. In conclusion, we propose a clear picture of how PP2ACdc55 functions affect the regulation of various MEN components, contributing to mitotic exit. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
11. Zds1 regulates PP2ACdc55 activity and Cdc14 activation during mitotic exit through its Zds_C motif.
- Author
-
Calabria, Ines, Baro, Barbara, Rodriguez-Rodriguez, Jose-Antonio, Russiñol, Nuria, and Queralt, Ethel
- Subjects
- *
MITOSIS , *CELLULAR control mechanisms , *ANAPHASE , *CYCLIN-dependent kinases , *CELL division , *PROTEIN kinases , *PLANTS - Abstract
At anaphase onset, highly active mitotic cyclin-dependent kinase (Cdk) is inactivated to promote exit from mitosis and completion of cytokinesis. The budding yeast Cdc14p phosphatase is a key mitotic regulator that counteracts cyclin-dependent kinase (Cdk) activity during mitotic exit. Separase, together with Zds1p, promotes the downregulation of the protein phosphatase 2A in conjunction with its Cdc55p regulatory subunit (PP2ACdc55) in early anaphase, enabling accumulation of phosphorylated forms of Net1p and release of Cdc14p from the nucleolus. Here we show that the C-terminal domain of Zds1p, called the Zds_C motif, is required for Zds1-induced release of Cdc14p, and the N-terminal domain of the protein might be involved in regulating this activity. More interestingly, Zds1p physically interacts with Cdc55p, and regulates its localization through the Zds_C motif. Nevertheless, expression of the Zds_C motif at endogenous levels cannot induce timely release of Cdc14p from the nucleolus, despite the proper (nucleolar) localization of Cdc55p. Our results suggest that the activity of PP2ACdc55 cannot be modulated solely through regulation of its localization, and that an additional regulatory step is probably required. These results suggest that Zds1p recruits PP2ACdc55 to the nucleolus and induces its inactivation by an unknown mechanism. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
12. The Positive Rhinovirus/Enterovirus Detection and SARS-CoV-2 Persistence beyond the Acute Infection Phase: An Intra-Household Surveillance Study.
- Author
-
Brotons, Pedro, Jordan, Iolanda, Bassat, Quique, Henares, Desiree, Fernandez de Sevilla, Mariona, Ajanovic, Sara, Redin, Alba, Fumado, Vicky, Baro, Barbara, Claverol, Joana, Varo, Rosauro, Cuadras, Daniel, Hecht, Jochen, Barrabeig, Irene, Garcia-Garcia, Juan Jose, Launes, Cristian, and Muñoz-Almagro, Carmen
- Subjects
CORONAVIRUSES ,SARS-CoV-2 ,COVID-19 ,RESPIRATORY infections ,REVERSE transcriptase polymerase chain reaction - Abstract
We aimed to assess the duration of nasopharyngeal severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA persistence in adults self-confined at home after acute infection; and to identify the associations of SARS-CoV-2 persistence with respiratory virus co-detection and infection transmission. A cross-sectional intra-household study was conducted in metropolitan Barcelona (Spain) during the time period of April to June 2020. Every adult who was the first family member reported as SARS-CoV-2-positive by reverse transcription polymerase chain reaction (RT-PCR) as well as their household child contacts had nasopharyngeal swabs tested by a targeted SARS-CoV-2 RT-PCR and a multiplex viral respiratory panel after a 15 day minimum time lag. Four-hundred and four households (404 adults and 708 children) were enrolled. SARS-CoV-2 RNA was detected in 137 (33.9%) adults and 84 (11.9%) children. Rhinovirus/Enterovirus (RV/EV) was commonly found (83.3%) in co-infection with SARS-CoV-2 in adults. The mean duration of SARS-CoV-2 RNA presence in adults' nasopharynx was 52 days (range 26–83 days). The persistence of SARS-CoV-2 was significantly associated with RV/EV co-infection (adjusted odds ratio (aOR) 9.31; 95% CI 2.57–33.80) and SARS-CoV-2 detection in child contacts (aOR 2.08; 95% CI 1.24–3.51). Prolonged nasopharyngeal SARS-CoV-2 RNA persistence beyond the acute infection phase was frequent in adults quarantined at home during the first epidemic wave; which was associated with RV/EV co-infection and could enhance intra-household infection transmission. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
13. SILAC-based phosphoproteomics reveals new PP2A-Cdc55-regulated processes in budding yeast.
- Author
-
Baro, Barbara, Játiva, Soraya, Calabria, Inés, Vinaixa, Judith, Bech-Serra, Joan-Josep, de LaTorre, Carolina, Rodrigues, João, Hernáez, María Luisa, Gil, Concha, and Barceló-Batllori, Silvia
- Subjects
- *
PHOSPHOPROTEIN phosphatases , *CELL proliferation , *CELL growth - Abstract
Background Protein phosphatase 2A (PP2A) is a family of conserved serine/threonine phosphatases involved in several essential aspects of cell growth and proliferation. PP2ACdc55 phosphatase has been extensively related to cell cycle events in budding yeast; however, few PP2ACdc55 substrates have been identified. Here, we performed a quantitative mass spectrometry approach to reveal new substrates of PP2ACdc55 phosphatase and new PP2A-related processes in mitotic arrested cells. Results We identified 62 statistically significant PP2ACdc55 substrates involved mainly in actin-cytoskeleton organization. In addition, we validated new PP2ACdc55 substrates such as Slk19 and Lte1, involved in early and late anaphase pathways, and Zeo1, a component of the cell wall integrity pathway. Finally, we constructed docking models of Cdc55 and its substrate Mob1. We found that the predominant interface on Cdc55 is mediated by a protruding loop consisting of residues 84–90, thus highlighting the relevance of these aminoacids for substrate interaction. Conclusions We used phosphoproteomics of Cdc55-deficient cells to uncover new PP2ACdc55 substrates and functions in mitosis. As expected, several hyperphosphorylated proteins corresponded to Cdk1-dependent substrates, although other kinases’ consensus motifs were also enriched in our dataset, suggesting that PP2ACdc55 counteracts and regulates other kinases distinct from Cdk1. Indeed, Pkc1 emerged as a novel node of PP2ACdc55 regulation, highlighting a major role of PP2ACdc55 in actin cytoskeleton and cytokinesis, gene ontology terms significantly enriched in the PP2ACdc55-dependent phosphoproteome. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
14. Zds1 regulates PP2A(Cdc55) activity and Cdc14 activation during mitotic exit through its Zds_C motif.
- Author
-
Calabria I, Baro B, Rodriguez-Rodriguez JA, Russiñol N, and Queralt E
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Amino Acid Motifs, Cell Cycle Proteins genetics, Cell Nucleolus genetics, Cell Nucleolus metabolism, Gene Expression Regulation, Fungal, Phosphorylation, Protein Binding, Protein Phosphatase 2 genetics, Protein Tyrosine Phosphatases genetics, Saccharomyces cerevisiae enzymology, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins genetics, Adaptor Proteins, Signal Transducing chemistry, Adaptor Proteins, Signal Transducing metabolism, Cell Cycle Proteins metabolism, Mitosis, Protein Phosphatase 2 metabolism, Protein Tyrosine Phosphatases metabolism, Saccharomyces cerevisiae cytology, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins chemistry, Saccharomyces cerevisiae Proteins metabolism
- Abstract
At anaphase onset, highly active mitotic cyclin-dependent kinase (Cdk) is inactivated to promote exit from mitosis and completion of cytokinesis. The budding yeast Cdc14p phosphatase is a key mitotic regulator that counteracts cyclin-dependent kinase (Cdk) activity during mitotic exit. Separase, together with Zds1p, promotes the downregulation of the protein phosphatase 2A in conjunction with its Cdc55p regulatory subunit (PP2A(Cdc55)) in early anaphase, enabling accumulation of phosphorylated forms of Net1p and release of Cdc14p from the nucleolus. Here we show that the C-terminal domain of Zds1p, called the Zds_C motif, is required for Zds1-induced release of Cdc14p, and the N-terminal domain of the protein might be involved in regulating this activity. More interestingly, Zds1p physically interacts with Cdc55p, and regulates its localization through the Zds_C motif. Nevertheless, expression of the Zds_C motif at endogenous levels cannot induce timely release of Cdc14p from the nucleolus, despite the proper (nucleolar) localization of Cdc55p. Our results suggest that the activity of PP2A(Cdc55) cannot be modulated solely through regulation of its localization, and that an additional regulatory step is probably required. These results suggest that Zds1p recruits PP2A(Cdc55) to the nucleolus and induces its inactivation by an unknown mechanism.
- Published
- 2012
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.