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A cross-sectional study was conducted on 92 female donkeys. Blood samples were collected, and the following parameters were evaluated: red blood cell (RBC), white blood cell, neutrophil, lymphocyte, monocyte (MON), eosinophil (EOS) and basophil counts, hemoglobin concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and the hematocrit (HCT), alanine aminotransferase (GPT), aspartate aminotransferase, total proteins, γ-glutamyl-transferase, alkaline phosphatase, creatinine, urea, and blood urea nitrogen and electrophoretic profile. Age (≥2 years ≤ 3 [very young], >3 years ≤ 10 [young], and >10 years ≤ 17 [adult]) and lactation (early lactation [≤3 months], middle [>3 months ≤ 6], and late lactation [>6 months]). Groups were independently analyzed using one-way analysis of variance or Kruskal–Wallis (post hoc test: Bonferroni's or Dunn's multiple test) tests; P was set as

Context: Yields from Brazilian beef-production systems do not always match the expected potential of a forage-based beef-production system. This efficiency is dependent on adjustments of grazing intensity and supplement utilisation to achieve higher bodyweight gain and lower methane emission. Therefore, more studies are necessary to evaluate the association between pasture management and supplement doses. Aims: The aim of the present study was to determine nutrient intake, nutrient digestibility, animal performance, carcass characteristics and enteric methane emissions of young Nellore bulls grazing Urochloa brizantha cv. Marandu pastures. Methods: One hundred and forty-eight yearling bulls (230 ± 17 kg) were randomly assigned to a grazing-by-supplementation strategy that was designed to allocate three different sward heights with differing levels of supplementation during the wet season. Treatment combinations were (1) low sward height with high supplementation (LH-HS, 15-cm sward height and supplementation at 0.6% of bodyweight (BW)); (2) low height with moderate supplementation (LH-MS, 15 cm and 0.3% BW); (3) moderate height with moderate supplementation (MH-MS, 25 cm and 0.3% BW); (4) moderate height with low supplementation (MH-LS, 25 cm and 0.1% BW); (5) high height with low supplementation (HH-LS, 35 cm and 0.1% BW); and (6) high height with no supplementation (HH-WS, 35 cm). Key results: Bulls in the HH groups had a greater herbage intake than did those in the LH groups (P < 0.01). Bulls in the LH-HS treatment resulted in a greater (P < 0.01) carcass average daily gain than that obtained with LH-MS, MH-LS or HH-WS treatment. Higher stocking rate with the LH treatment resulted in greater gains per hectare in terms of both BW and carcass (P < 0.01). Carcass yield was greater for bulls maintained with the LH-HS treatment (54.3% BW). Higher enteric methane emissions were observed from bulls under the HH treatments (P < 0.01). Conclusions: Comparing carcass gains per hectare and low methane emissions, the present study indicated that pasture management towards a low sward height combined with 0.3% or 0.6% BW supplementation can result in a greater nutrient utilisation efficiency of bulls. Implications: Results provided information to obtain better gains per animals and area, also decreasing methane emission of beef cattle production system. Brazilian beef production produces animal protein mainly by using fibrous feeds such as tropical grasses, which reduces the cost of production. However, grass production is variable during the year, so it is important to use supplementation to fulfil animal requirements. We aimed to determine the effects of different combinations of sward heights and supplementation levels on animal production variables. It was found that pasture management for different heights needs different supplementation levels to attend to animal requirements during the dry season, resulting in great production and probably positive economy to the owner. [ABSTRACT FROM AUTHOR]

ABSTRACT: Extreme precipitation events are widely thought to intensify in a warmer atmosphere through the Clausius‐Clapeyron equation. The temperature‐extreme precipitation scaling was proposed to analyse the temperature dependency of short‐duration extreme precipitation and since then, the concept has been widely used in climatology. Bao et al. (2017) suggest that the apparent scaling reflects not only how surface air properties affect extreme precipitation, but also reflects how synoptic conditions and localized cooling due to the storm itself affect the scaling – implying two‐way causality. We address here critical issues of this paper and provide evidence that dew point temperature drives extreme precipitation, with the direction of causality reversed only for the storm's peak intensity. This physical inference may serve as a basis to better quantify scaling rates and to help establish the relationship between extreme precipitation and environmental conditions in the current climate, and thereby provide insights into future changes to precipitation extremes due to climate change. [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the effect of stockpiled forage type and protein supplementation on VFA production, serum metabolites, and BW in yearling beef heifers. Over 2 yr, spring-born, Angus crossbred yearling beef heifers (n = 42; 305 ± 2.9 kg initial BW) were randomly assigned to 1 of 3 forage pasture types: 1) endophyteinfected tall fescue [TF; Schedonorus arundinaceus (Schreb.) Dumort], 2) a big bluestem (Andropogon gerardii Vitman) and indiangrass (Sorghastrum nutans L.) combination (BI), or 3) switchgrass (SG; Panicum virgatum L.). Each pasture was then randomly assigned to receive either 1 of 2 isonitrogenous CP treatments: 1) 0.68 kg·heifer-1·d-1 of dried distiller's grains with solubles (DDGS; 28% CP and 88% TDN) or 2) 0.22 kg·heifer-1·d-1 of blood meal and fish meal (BF; 72.5% CP and 69.5% TDN), resulting in a 3 × 2 factorial arrangement of treatments. Treatments were initiated in January and terminated in April in both years of the study. Body weights and blood samples were collected approximately every 28 d from initiation of grazing until the end of the trial. Heifer BW change from January to February and overall BW change were greater (P < 0.01) for TF heifers. However, BW change from March to April was not different (P = 0.84) among forage types. Supplement type did not influence (P ≥ 0.13) BW or BW change from January to February and from January to April; however, heifers fed DDGS had greater (P = 0.03) BW gain from March to April. Heifer BW change from February to March exhibited (P < 0.05) a forage type × supplement interaction, with BF-fed heifers gaining more BW on BI pastures than DDGSfed heifers. Serum glucose concentrations, ruminal acetate, and the acetate:propionate ratio were greater (P ≤ 0.04) for SG heifers. However, circulating serum NEFA and urea N (SUN) concentrations were not different (P ≥ 0.85) among forage types. Serum glucose and NEFA concentrations were not influenced (P ≥ 0.61) by supplement type. Circulating SUN concentrations were greater (P < 0.01) in BF-supplemented heifers. Ruminal acetate tended to be greater (P = 0.09) and butyrate concentrations were greater (P < 0.01) for BF-supplemented heifers. The acetate:propionate ratio was not influenced (P = 0.15) by supplement type. These results suggest that a compensatory gain period prior to breeding would be needed for these native warm-season species to be a viable opportunity for growing and developing replacement heifers in the southeastern United States. [ABSTRACT FROM AUTHOR]

Echinacea pallida (EPAL), also known as pale purple coneflower, is a herbaceous flowering plant with immune-enhancement and antioxidative properties. The effect of EPAL on the reproductive performance, serum biochemistry and haematological parameters of rabbit does has been studied here. A total of 100, 21-week-old Grimaud rabbit does, were randomly assigned to two groups. One group was fed a basal diet supplemented with 3 g EPAL/kg diet (Echinacea group, E), while the other was fed the basal diet without the supplementation (control group, C). The reproductive performance of the does was not affected by the treatment (P>0.05). The haematological parameters of pregnant rabbits showed that there was no interaction between gestation day and treatment. The EPAL supplementation induced a reduction (-47.3%) in the basophil cell rate (0.55% and 0.29%, for the control and treatment groups, respectively; P = 0.049). The gestation day significantly affected most of the haematological parameters (P<0.05). The white blood cell counts declined progressively after day 14. The mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, red cell distribution width, mean platelet volume and eosinophils increased steadily throughout the study, and reached a maximum value on day 28. The red blood cells, haemoglobin, haematocrit, mean corpuscular volume and neutrophils increased slightly up to day 14, and then subsequently decreased progressively until day 28. The lymphocytes and platelet distribution width decreased until day 14, and then increased to a maximum value on day 28. No significant effect of gestation day or treatment was observed on the blood serum chemistry. As far as the immune parameters are concerned, no significant differences were observed between groups, while a significant effect of gestation day was observed for lysozymes (6.02 v. 7.99 v. 1.91; for 0, 14 and 28 days, respectively; P = 0.014). In conclusion, a lack of effect of EPAL has been observed. In fact, no impacts of EPAL have been observed on the reproductive or haematological parameters of the does. The effects of dietary supplementation with EPAL on the performances, bacterial community, blood parameters and immunity in growing rabbits are reported in the second part of this study. [ABSTRACT FROM AUTHOR]

Echinacea pallida (EPAL), a herbaceous flowering plant with immunomodulatory properties, has been chosen to determine the pre- and post-supplementary effects on the growth performances, bacterial community, blood parameters and immunity of growing rabbits. The same Grimaud does (14-week-old) from the studied in the first part of this study were randomly divided into two groups (n = 50/group). The first group was fed a basal diet without supplementation (Control group, C) while the another group was fed a basal diet supplemented with 3 g EPAL/kg diet (Echinacea group, E). From the second parturition, 80 weaned kits (40 from the C does and 40 from the E does) were randomly assigned to four groups of 20 animals each and were fed a growing commercial diet supplemented with or without a 3 g EPAL/kg diet: the CC group (rabbits from the C does fed the control diet), CE group (rabbits from the C does fed the supplemented diet), EC (rabbits from the E does fed the control diet) and EE group (rabbits from the E does fed the supplemented diet). The dietary EPAL treatment did not affect the growth performance. Ten fattening rabbits from each group were selected to evaluate the bacterial community and blood parameters, while the remaining rabbits (n = 10/group) were used to study phagocytosis and the humoral immune response. The variability was evaluated from hard faeces at 35, 49 and 89 days, and the caecal content at 89 days. The variability of the bacterial community of the EE group was higher than that of the other groups. The phagocytic activity was higher in the CE and EE groups than in the CC and EC ones (30.9 and 29.7 v. 21.2 and 21.8%; P<0.05), whereas no statistically significant difference was observed for the blood parameters or humoral immune response against vaccination (rabbit haemorrhagic disease virus) at 95 days old which the serum was collected at 88, 102, 109, 116 and 123 days old. In conclusion, no impact of EPAL dietary supplementation has been observed on the growth performances, bacterial community, blood parameters or humoral immune responses in growing rabbits, except for an increase in phagocytic activities. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the performance of beef heifers on Stapf. cv. Marandu grass pastures that were managed at three herbage allowances under continuous grazing with a variable stocking rate and were provided with mineral mix supplement or supplements with energy and protein. Seventy-two test heifers and 48 put-and-take heifers were used in a randomised design in a 3 × 2 factorial arrangement [three herbage allowances (1.5, 2.0, and 2.5 kg of green dry matter/kg of bodyweight, and two supplementation levels (mineral mix or 0.3% bodyweight of a mineral-protein-energy supplement)], with two replicates per treatment (paddocks), to maintain the preestablished forage allowance. No significant difference was observed in the gain per hectare as a function of the herbage allowance, but 2.5 kg green dry matter/kg bodyweight provided the greatest average daily gain of bodyweight (0.605 kg/ animal/day). The energy/protein supplementation provided the greatest average daily gain compared with animals fed only the mineral mix. The supplements in the diet of beef heifers fed at 0.3% of bodyweight increased bodyweight gains without compromising the gain per hectare at different herbage allowances. [ABSTRACT FROM AUTHOR]

Very large fires (VLFs) have important implications for communities, ecosystems, air quality and fire suppression expenditures. VLFs over the contiguous US have been strongly linked with meteorological and climatological variability. Building on prior modelling of VLFs (>5000 ha), an ensemble of 17 global climate models were statistically downscaled over the US for climate experiments covering the historic and mid-21st-century periods to estimate potential changes in VLF occurrence arising from anthropogenic climate change. Increased VLF potential was projected across most historically fire-prone regions, with the largest absolute increase in the intermountain West and Northern California. Complementary to modelled increases in VLF potential were changes in the seasonality of atmospheric conditions conducive to VLFs, including an earlier onset across the southern US and more symmetric seasonal extension in the northern regions. These projections provide insights into regional and seasonal distribution of VLF potential under a changing climate, and serve as a basis for future strategic and tactical fire management options. Very large fires (VLFs) have important implications for ecosystems, air quality and fire suppression expenditures. Climate change projections suggest an increase in conditions conducive to VLF potential across most historically fire-prone regions of the US as well as changes in the seasonality of such conditions. [ABSTRACT FROM AUTHOR]

Endocannabinoids have analgesic/anti-inflammatory properties. The biology of endocannabinoids, their receptors, signalling mechanisms and role in the regulation of physiological processes have been extensively reviewed. This review focuses on the role of palmitoylethanolamide (PEA), an endogenous fatty acid amide analogue of the endocannabinoid anandamide, in tissue protective mechanisms. PEA was first identified almost five decades ago in lipid extracts of various natural products, and its anti-inflammatory and antinociceptive effects were established later. Evidence exists that PEA is synthesised during inflammation and tissue damage and a number of beneficial effects, including the relief of inflammation and pruritus, have been shown to be useful in the control of neurogenic and neuropathic pain. The postulated hypotheses as to the mode of action of PEA include a possible local autacoid-like mediator activity regulating mast-cell activity and putative activation of cannabinoids and vanilloid TRPVI receptors via "entourage" effects. The large number of scientific investigations into the effects of PEA and PEA-related compounds has given rise to new therapeutic opportunities. In spite of the multitude of therapies currently employed to control inflammation, pain, pruritus and tissue damage, the possibility of using a natural compound, such as PEA to manipulate endogenous protective mechanisms may be considered a beneficial novel therapeutic strategy in veterinary medicine. [ABSTRACT FROM AUTHOR]

Mepartricin is a semi-synthetic macrolide antibiotic developed as a drug for the treatment of benign prostatic hyperplasia (BPH) in human patients. In the present study, aged rats are used as an experimental model to evaluate the effects of mepartricin on circulating hormone concentrations and prostate receptor concentrations, to compare these possible effects with clinical findings observed in long-term treated dogs. Fifty-six aged male rats were randomly divided into four experimental groups treated orally with 0 (group 1), 2 mg (group 2), 5 mg (group 3) and 20 mg (group 4) mepartricin/kg of body weight. for 28 days respectively. Serum oestradiol and testosterone concentrations were measured by radio-immune-assays methods. Binding assays were used to measure the prostate concentrations of oestrogen receptors (ER), androgen receptors (AnR), α1-adrenergic receptor ( α1-AR), and β-adrenerergic receptor ( β-AR) subtypes. Mepartricin induced a significant reduction of prostate weight and serum oestradiol concentrations. Serum testosterone concentrations were unaffected. The treatment induced a significant down-regulation of ER concentrations ( P < 0.05) and a significant up-regulation of AnR ( P < 0.05) in rat prostate. Mepartricin induced a significant ( P < 0.05) dose-dependent up-regulation of α1-AR and β2-AR. In contrast, the concentration of β3-ARs was significantly decreased ( P < 0.05) in treated animals. The increase in prostate β2-AR concentrations observed in subjects treated with mepartricin may be a favourable element in the evolution of BPH, because of the role exerted by these receptors in the control of prostatic smooth muscle relaxation. Curiously, β3-AR concentrations were significantly reduced in treated animals. Data collected suggest that the prostatic β-AR expression might be strongly influenced by oestrogen deprivation (mepartricin treatment); therefore, the combination of oestrogen suppression (mepartricin) and adrenergic suppression ( α1-AR blockers) may be proposed as a possible nonhormonal therapeutic strategy for the treatment of benign prostatic hyperplasia in dogs. [ABSTRACT FROM AUTHOR]

In order to identify possible peripheral markers of illegal treatments with growth-promoting agents in veal calves, β-adrenergic receptor ( β-AR) and glucocorticoid receptor (GR) concentrations were measured in lymphocytes of 12 male Friesian crossbred calves (six controls and six treated). The animals received a cocktail of anabolic and re-partitioning agents [17 β-oestradiol: 3 × 10 mg intramuscular (i.m.) doses at 17-day intervals; dexamethasone sodium phosphate: 4 mg/day for 6 days and 5 mg/day for six further days dissolved in milk; and clenbuterol: 20 μg/kg/day dissolved in milk for the last 40 days before slaughter]. Blood samples were collected by venipuncture at different time points and lymphocytes were isolated by density gradient centrifugation. Lymphocyte β-AR and GR levels were measured by binding assays. Treatment with re-partitioning agents caused a significant down-regulation of lymphocyte β-ARs 19 days after the beginning of clenbuterol administration and at day 55 (after dexamethasone withdrawal, just before slaughter). This phenomenon was partially reversed at day 50, after dexamethasone administration, at which time a significant decrease in GR concentrations also occurred. For both types of receptors, no significant changes in the dissociation constant values were observed at any time point. Lymphocytes express measurable concentrations of β-ARs and GRs and the measurement of receptor levels highlights the fluctuation of receptor expression due to the dynamic interaction of the drugs used in combination. Lymphocyte receptor determination could therefore be included in a battery of biological assays to detect illegal treatments with anabolic agents in veal calves in the light of a multivariate approach. [ABSTRACT FROM AUTHOR]

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The objective was to determine the effect of stockpiled forage type and protein supplementation on VFA production, serum metabolites, and heifer BW. Over 2 yr, spring-born, crossbred heifers (n = 45) were stratified by BW at weaning and randomly assigned to 1 of 3 forage treatments: 1) endophyte-infected tall fescue (TF; Festuca arundinacea), 2) big bluestem (Andropogon gerardi Vitman) and indiangrass (Sorghastrum nutans L.) combination (BI), or 3) switchgrass (SG; Panicum virgatum L.). Forage treatments were then randomly assigned to receive 1 of 2 supplement types: 1) 0.68 kg·heifer-1·d-1 of dried distiller's grains with solubles (DDGS; 28% CP, 74% RUP, and 88% TDN) or 2) 0.22 kg·heifer-1·d-1 of blood meal and fish meal (BF; 72.5% CP, 67.5% RUP, and 69.5% TDN), resulting in a 3 × 2 factorial arrangement of treatments. Treatments were initiated every year in January and terminated in April at the onset of a 60-d breeding season. Body weights and serum samples were collected approximately every 28 d from initiation of grazing until breeding. Serum glucose concentrations were greater (P = 0.05) for heifers grazing SG. Circulating NEFA concentrations were greater (P = 0.04) in TF heifers. Serum urea N (SUN) was not influenced (P = 0.37) by forage type. Ruminal acetate concentration and acetate:propionate ratio were greater (P = 0.04) in heifers grazing SG pastures. Heifer BW change from January to February and overall BW change were greater (P < 0.01) for TF heifers. However, BW change from March to April was not different (P = 0.84) among forage types. Serum glucose and SUN concentrations exhibited (P < 0.05) supplement type × sampling time interactions. Ruminal acetate tended to be greater (P = 0.09) and butyrate concentrations were greater (P < 0.01) for BF-supplemented heifers. The acetate:propionate ratio was not influenced (P = 0.15) by supplement type. Supplement type did not influence (P > 0.13) BW or BW change from January to February and January to April; however, heifers fed DDGS gained (P = 0.03) more BW from March to April. Heifer BW change from February to March exhibited (P < 0.05) a forage type × supplement interaction with BF heifers gaining more BW on BI pastures than DDGS heifers. Overall, developing heifers on low-quality native forages initially delayed BW gain; however, heifers seemed to quickly adapt to the lower-quality forage, resulting in similar BW gains in March and April. [ABSTRACT FROM AUTHOR]

The article presents a study that aims to measure the concentration of lymphocyte muscarinic receptors (MR), platelet α2-adrenergic receptors and blood monoamine levels in ageing dogs. The study used 27 healthy dogs of both sexes divided into two groups: adults and old dogs. Blood samples were collected and the plasma catecholamines and serum serotonin was measured. The results suggest that both MR and serotonin levels represent appropriate marker of ageing in dogs.

The article focuses on a study that investigates the effects of human-dog interaction in order to study its impact on dog behaviour and salivary cortisol levels. For the study 19 long-term sheltered dogs of both sexes recruited at the Public Animal Shelter of Turin were divided into two behaviorally homogeneous groups. The results from the study indicated that some environmental changes have a positive effect upon the behaviour and the welfare of sheltered dogs.

The article focuses on a study that analyzes the effects of herbal extracts on blood prostaglandins, leukotrienes and thromboxanes in the horse. For this study, seventeen horses, with an average age of 14.1 years and medium heart girth of 188 cm, were used. The results of the study indicates that there are no differences in the serum concentrations of blood prostaglandins, leukotrienes and thromboxanes between horses with chronical inflammatory process and healthy horses.

The article focuses on a study that identifies whether the TRPV1 vanilloid receptor is expressed by MCF-7 cells since the receptor subtypes for the cannabinoids CB1 and CB2 have already been characterized in their plasma membranes. For this study, MCF-7 cells were plated and cultured to get the number of cells needed to perform binding assay and then re-suspended in binding buffer. The study identified the TRPV1 receptor expression and functionality in epithelial cells of companion animals.

The article presents a research related to the effects of short term transportation and housing on hormonal levels and on lymphocyte glucocorticoid and β-andrenergic receptor levels in beef calves. Thirty-two male calves were blood sampled at 6, 12 and 18 months before and after transportation to the slaughterhouse. Hormone levels were determined by radioimmunoassay. The study confirms the activation of the hypothalamic-pituitary-adrenal axis system in short-term transported calves.

The article focuses on a study that analyzes the aliamide effect of Giupamid, an amide compound, in the well-established in vitro model of mast cell degranulation. The study showed that Glupamid is very active in reducing serotonin release in a concentration-dependent fashion. This characteristic of Glupamid demonstrates that the compound is active as an aliamide and strengthens its potential therapeutic use as a disease-oriented approach to osteoarthritis.

The article focuses on a study that analyzes the mechanism of action of aliamides in dogs and cats. In this study the aliamide parent molecule palmitoylethanolamide, and its topically-active congener adelmidrol, was tested on cutaneous mast cells of dogs and cats. Densitometric analysis performed on skin biopsies of allergic cats and canine skin wounds showed an increase in the staining intensity of cutaneous mast cells suggesting that aliamides are effective in decreasing degranulation.