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Abstract Endometriosis is a chronic systemic disease, which results in endometrial-type tissue growing outside the uterus, and affects approximately 10% of reproductive-aged women worldwide. Its aetiology is poorly understood, and there is currently no long-term cure. Development and persistence of the disease depend on several coexisting factors including the vaginal microbiome. However, the role played by this important entity in endometriosis and its systemic involvement is not fully understood. Here, we investigated the vaginal microbiota, the serum and urine glycome, and antibody glycosylation in endometriosis patients. We reveal an endometriosis-specific vaginal microbiota in patients, being distinct from that present in a control group. Endometriosis patients were typified by a loss of the dominant Lactobacillus species, i.e. Lactobacillus iners, increased bacterial diversity and the presence of species such as Anaerococcus senegalensis, Prevotella jejuni, Porphyromonas bennonis and Anaerococcus octavius. The presence of trigalactosylated and triantennary serum glycans and urine core fucosylated mono-antennary glycans from IgG correlated with the vaginal presence of the bacterium A. senegalensis in endometriosis patients. Urine glycans did not differ in endometriosis, but urine IgG identified four novel sulfated glycans differing from serum IgG indicating functional relevance. Our findings contribute to understanding the relationships between the vaginal microbiota and the serum and urine glycome on the one hand, and endometriosis on the other. Further functional studies are warranted.

Dzieci ulicy na świecie i w Polsce doświadczają wielu problemów. Jednym z nich jest wykluczenie edukacyjne, które marginalizuje tę grupę dzieci i młodzieży w obszarze edukacji. Celem naszego artykułu jest analiza czynników składających się na ten rodzaj wykluczenia społecznego. Streetworkerzy z Krakowa wśród których przeprowadzono badania w swoich opiniach wyszczególnili trzy obszary: 1) dysfunkcjonalność szkoły (brak wiedzy o uczniach-dzieciach ulicy, realizacja programu nauczania z pominięciem słabszego ucznia, zaniedbywanie zasady przystępności w nauczaniu); 2) relacja nauczyciel-dzieci ulicy (stygmatyzacja uczniów z rodzin dysfunkcyjnych, nieuzasadnione stawianie ocen niedostatecznych, wyśmiewanie i poniżanie) oraz 3) relacja rówieśnicy-dzieci ulicy (przemoc, kłótnie, konflikty rówieśnicze).

Praca w grupach dzieci i młodzieży zdefiniowanych włóczęgowskim stylem życia i utrwaloną egzystencją w przestrzeni miasta prowadzona jest metodą streetworkingu przez odpowiednio przygotowanych i przeszkolonych streetworkerów. Nie tylko względnie stałe wartości składające się na etykę pracy, ale interesująca jest również refleksja nad sposobem przeżywania pracy przez jednostki i grupy, który może być pozytywny lub negatywny. Artykuł ma na celu prezentację determinantów satysfakcji z pracy streetworkerów wśród dzieci ulicy w Polsce analizowanych w kategorii pasji pedagogicznej rozumianej jako towarzyszenie. W narracjach badanych streetworkerów jest to pasja dla dobra dzieci ulicy prezentowana w trzech formach: pomoc, obecność w środowisku oraz intencjonalność spotkania.

The article addresses issues related to the experience of helplessness of a child’s suffering. The theoretical part presents the perspective of understanding the helplessness of a child experiencing sexual violence. The methodological part introduces the basic assumptions of the research project. The subject of the study was the helplessness of a victim of sexual violence, and the purpose was to identify significant determinants showing the helplessness of suffering of a child experiencing sexual violence and their description in the experience of study participants. The study used the narrative method following content analysis. The research problem took the form of a question: What is the experience of helpless suffering of a child in the narratives of post-graduate students preparing to work with an abused child? The research was conducted in 2019 among 23 post-graduate students preparing for counseling work. Obtained research results have allowed the emergence of subcategories within the examined categories that show the specificity and value of the helplessness of the suffering of the child described by the respondents - victims of sexual violence. In addition, the conclusions of the study are valuable for pedagogical practice, especially in the scope of enriching the educational offer for the teaching profession and teacher in relation to diagnostic, intervention and support activities for people with experience of violence.

This article addresses the issue of the competence of counsellors working with people experiencing sexual violence. The theoretical part of the article describes the basic concepts related to institutional (professional) counselling and describes a professional counsellor and his/her competences in a descriptive way. The subject of the research includes the competences of counsellors in the field of working with people who have experienced sexual violence. The purpose of the study was determining and describing their competences – both organizational and professional ones – from the perspective of the experience of individual listeners preparing for counselling work. The study uses a narrative method with content analysis. The research problem took the form of the following question: what are the competences of a counsellor in accompanying a sexually abused person from the perspective of the personal experience of future counsellors? The research was conducted in 2019 among 23 postgraduate students preparing for counselling work. The results made it possible to select subcategories within the examined categories that show the specificity and value of the competences described by the respondents, and allowed us to emphasize the importance of the interdisciplinary knowledge and skills of counsellors. In addition, the conclusions of the study are valuable for pedagogical practice, especially in terms of enriching the offer of studies addressed to counsellors working with people who have experienced sexual violence.

Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach. In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers. Here, we demonstrate that the level of H. pylori and H. suis binding to human and pig gastric mucins varies between individuals with species dependent specificity. The binding optimum of H. pylori is at neutral pH whereas that of H. suis has an acidic pH optimum, and the mucins that H. pylori bind to are different than those that H. suis bind to. Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples. H. suis binding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose. Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates. Together with that H. suis bound to DNA (used as a proxy for acidic charge), we conclude that H. suis has two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures. Identification of the glycan structures H. suis interacts with can contribute to development of therapeutic strategies alternative to antibiotics.

Praca w grupach dzieci i młodzieży zdefiniowanych włóczęgowskim stylem życia i utrwaloną egzystencją w przestrzeni miasta prowadzona jest metodą streetworkingu przez odpowiednio przygotowanych i przeszkolonych streetworkerów. Nie tylko względnie stałe wartości składające się na etykę pracy, ale interesująca jest również refleksja nad sposobem przeżywania pracy przez jednostki i grupy, który może być pozytywny lub negatywny. Artykuł ma na celu prezentację determinantów satysfakcji z pracy streetworkerów wśród dzieci ulicy w Polsce analizowanych w kategorii pasji pedagogicznej rozumianej jako towarzyszenie. W narracjach badanych streetworkerów jest to pasja dla dobra dzieci ulicy prezentowana w trzech formach: pomoc, obecność w środowisku oraz intencjonalność spotkania.

Gastric carcinoma MKN45 cells stably transfected with the full-length ST3GAL4 gene were characterised by glycomic and sialoproteomic analysis. Complementary strategies were applied to assess the glycomic alterations induced by ST3GAL4 overexpression. The N- and O-glycome data were generated in two parallel structural analyzes, based on PGC-ESI-MS/MS. Data on glycan structure identification and relative abundance in ST3GAL4 overexpressing cells and respective mock control are presented. The sialoproteomic analysis based on titanium-dioxide enrichment of sialopeptides with subsequent LC-MS/MS identification was performed. This analysis identified 47 proteins with significantly increased sialylation. The data in this article is associated with the research article published in Biochim Biophys Acta “Glycomic analysis of gastric carcinoma cells discloses glycans as modulators of RON receptor tyrosine kinase activation in cancer” [1]. Keywords: N-glycome, O-glycome, Gastric cancer, Sialyltransferase, Sialoproteome

Niewielkiego formatu książka A. Grün’a podejmuje problem przebaczenia i pojednania, temat wprawdzie od dawna już na różne sposoby przedstawiany przez wybitnych teologów, a jednak na tyle ważny, że wart uczynienia z tych dwóch słów tytułu książki. Grün natomiast umieszcza je w podtytule, a całą swoją uwagę kieruje na źródło tej problematyki, na serce człowieka i książkę opatruje tytułem: Przebacz samemu sobie.

Dzieci ulicy na świecie i w Polsce doświadczają wielu problemów. Jednym z nich jest wykluczenie edukacyjne, które marginalizuje tę grupę dzieci i młodzieży w obszarze edukacji. Celem naszego artykułu jest analiza czynników składających się na ten rodzaj wykluczenia społecznego. Streetworkerzy z Krakowa wśród których przeprowadzono badania w swoich opiniach wyszczególnili trzy obszary: 1) dysfunkcjonalność szkoły (brak wiedzy o uczniach-dzieciach ulicy, realizacja programu nauczania z pominięciem słabszego ucznia, zaniedbywanie zasady przystępności w nauczaniu); 2) relacja nauczyciel-dzieci ulicy (stygmatyzacja uczniów z rodzin dysfunkcyjnych, nieuzasadnione stawianie ocen niedostatecznych, wyśmiewanie i poniżanie) oraz 3) relacja rówieśnicy-dzieci ulicy (przemoc, kłótnie, konflikty rówieśnicze).

Since the beginning of the history of the world, the truth about man as a being in relation has been known. In a wide range of relations we discover two categories of its meanings. The first one, marked with superficiality and self-interest, sets up the relations between two persons in the sphere of being closed to life. The second one, characterized by openness, another person’s good and the attitude of acceptance, aims at living and tasting life as a gift. The second type of relation defines pedagogical relation as a shared way of an educator and a pupil towards the height of humanity.Relation with another man constitutes the first step in evangelical pedagogy, where Jesus is shown as the One, who goes to meet man and seeks him in various moments and events of his fate. To specify these deliberations, two biblical pericops – “The Rich Young Man” (Matthew 19, 16–22) and “The First Four Disciples Are Called” (Mark 1, 16–22) – may prove helpful. Building a pedagogical relation in these evangelical texts between Jesus – the Educator and the pupil is revealed in four stages: intentionality in building relations, ability to participate in an educational situation, willpower to get to know the pupil and a project for one’s own life. In addition, a universal message for contemporary education is shown in each of these stages.

The phosphor powders of Ca(m/2)−xEuxSi12−(m+n)Alm+nOnN16−n (m = 1.6, n = 0.8, x in the range of 0–0.08) were synthesized by means of a solid state reaction in flowing nitrogen in a carbon resistant furnace and the influence of Eu concentration on the crystal structure and photoluminescent properties was thoroughly studied. The optical properties of selected α-sialon:Eu2+ samples at temperatures in the range of 10 to 500 K and pressures up to 240 kbar are presented. The crystal lattice parameters were affected by doping with europium and some increase of the unit cell volume was observed up to 6 mol % of Eu. The higher concentration of europium led to subtle changes in the overall structure of the produced sialon phosphors. It was shown that the chemical composition of Ca, Eu-α-sialon phosphor was slightly different from the designed one and the phosphor powders were contaminated by AlN. The phosphor particle surface showed significant europium and oxygen enrichment with Eu3+ but below the thin surface layer Eu2+ was dominant and higher nitrogen content was observed. After examination of absorption, excitation, and emission spectra it was found that the emission peak position shifted toward longer wavelengths with rising Eu2+ concentration from 565 nm (0.1 mol % Eu2+) to 585 nm (10 mol % Eu2+). The quantum yield of the phosphors reached the maximum at a rather low concentration of 4 mol % of Eu. Excitation spectra depend on the monitored wavelength which is typical for multisite Eu2+. The existence of many Eu2+ sites in the sample was supported by the dependence of the decay time on the monitored wavelength.

Glycosylation of immunoglobulin G (IgG) influences IgG effector function by modulating binding to Fc receptors. To identify genetic loci associated with IgG glycosylation, we quantitated N-linked IgG glycans using two approaches. After isolating IgG from human plasma, we performed 77 quantitative measurements of N-glycosylation using ultra-performance liquid chromatography (UPLC) in 2,247 individuals from four European discovery populations. In parallel, we measured IgG N-glycans using MALDI-TOF mass spectrometry (MS) in a replication cohort of 1,848 Europeans. Meta-analysis of genome-wide association study (GWAS) results identified 9 genome-wide significant loci (P