48 results on '"Awad, Milena"'
Search Results
2. Paeniclostridium (Clostridium) sordellii–associated enterocolitis in 7 horses
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Nyaoke, Akinyi C, Navarro, Mauricio A, Fresneda, Karina, Diab, Santiago S, Moore, Janet, Lyras, Dena, Awad, Milena, and Uzal, Francisco A
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Digestive Diseases ,Emerging Infectious Diseases ,Good Health and Well Being ,Animals ,Clostridium ,Clostridium Infections ,Clostridium sordellii ,Diagnosis ,Differential ,Enterocolitis ,Horse Diseases ,Horses ,Intestine ,Large ,Intestine ,Small ,colitis ,enteritis ,enterocolitis ,horses ,Paeniclostridium sordellii ,Zoology ,Veterinary Sciences - Abstract
Enteric disease in horses may be caused by a variety of microorganisms, including several clostridial species. Paeniclostridium sordellii (previously Clostridium sordellii) has been frequently associated with gas gangrene in humans and several animal species, including horses. However, its role in enteric diseases of animals has not been fully determined. We describe herein 7 cases of enteric disease in horses associated with P. sordellii infection. Grossly, the small and/or large intestines were necrotic, hemorrhagic, and edematous. Microscopically, there was severe mucosal necrosis and hemorrhage of the small and/or large intestine of all horses. P. sordellii was isolated and/or demonstrated by immunohistochemistry and/or PCR in the intestine of all horses. All other known causes of enteric disease in horses were ruled out in these 7 cases. P. sordellii should be considered among the differential diagnoses in cases of enteric disease in horses.
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- 2020
3. Cephamycins inhibit pathogen sporulation and effectively treat recurrent Clostridioides difficile infection
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Srikhanta, Yogitha N., Hutton, Melanie L., Awad, Milena M., Drinkwater, Nyssa, Singleton, Julie, Day, Sophie L., Cunningham, Bliss A., McGowan, Sheena, and Lyras, Dena
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- 2019
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4. Towards an understanding of the role of Clostridium perfringens toxins in human and animal disease
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Uzal, Francisco A, Freedman, John C, Shrestha, Archana, Theoret, James R, Garcia, Jorge, Awad, Milena M, Adams, Vicki, Moore, Robert J, Rood, Julian I, and McClane, Bruce A
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Microbiology ,Biological Sciences ,Foodborne Illness ,Emerging Infectious Diseases ,Infectious Diseases ,Digestive Diseases ,2.2 Factors relating to the physical environment ,Aetiology ,Infection ,Animal Diseases ,Animals ,Bacterial Toxins ,Clostridium Infections ,Clostridium perfringens ,Gas Gangrene ,Humans ,Intestines ,animal disease ,avian necrotic enteritis ,enterocolitis ,enterotoxemia ,food poisoning ,gas gangrene ,human disease ,toxins ,Medical Microbiology - Abstract
Clostridium perfringens uses its arsenal of >16 toxins to cause histotoxic and intestinal infections in humans and animals. It has been unclear why this bacterium produces so many different toxins, especially since many target the plasma membrane of host cells. However, it is now established that C. perfringens uses chromosomally encoded alpha toxin (a phospholipase C) and perfringolysin O (a pore-forming toxin) during histotoxic infections. In contrast, this bacterium causes intestinal disease by employing toxins encoded by mobile genetic elements, including C. perfringens enterotoxin, necrotic enteritis toxin B-like, epsilon toxin and beta toxin. Like perfringolysin O, the toxins with established roles in intestinal disease form membrane pores. However, the intestinal disease-associated toxins vary in their target specificity, when they are produced (sporulation vs vegetative growth), and in their sensitivity to intestinal proteases. Producing many toxins with diverse characteristics likely imparts virulence flexibility to C. perfringens so it can cause an array of diseases.
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- 2014
5. Functional analysis of an feoB mutant in Clostridium perfringens strain 13
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Awad, Milena M., Cheung, Jackie K., Tan, Joanne E., McEwan, Alastair G., Lyras, Dena, and Rood, Julian I.
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- 2016
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6. Paeniclostridium sordellii and Clostridioides difficile encode similar and clinically relevant tetracycline resistance loci in diverse genomic locations
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Vidor, Callum J., Bulach, Dieter, Awad, Milena, and Lyras, Dena
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- 2019
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7. Antibiotic resistance, virulence factors and genetics of Clostridium sordellii
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Vidor, Callum, Awad, Milena, and Lyras, Dena
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- 2015
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8. Necrotic Enteritis in Chickens Associated with Clostridium sordellii
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Rimoldi, Guillermo, Uzal, Francisco, Chin, R. P., Palombo, Enzo A., Awad, Milena, Lyras, Dena, and Shivaprasad, H. L.
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- 2015
9. Comparing the identification of Clostridium spp. by two Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) mass spectrometry platforms to 16S rRNA PCR sequencing as a reference standard: A detailed analysis of age of culture and sample preparation
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Chean, Roy, Kotsanas, Despina, Francis, Michelle J., Palombo, Enzo A., Jadhav, Snehal R., Awad, Milena M., Lyras, Dena, Korman, Tony M., and Jenkin, Grant A.
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- 2014
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10. Expression of the large clostridial toxins is controlled by conserved regulatory mechanisms
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Carter, Glen P., Larcombe, Sarah, Li, Lucy, Jayawardena, Darshani, Awad, Milena M., Songer, J. Glenn, and Lyras, Dena
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- 2014
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11. Opioid Analgesics Stop the Development of Clostridial Gas Gangrene
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Chakravorty, Anjana, Awad, Milena M., Hiscox, Thomas J., Cheung, Jackie K., Choo, Jocelyn M., Lyras, Dena, and Rood, Julian I.
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- 2014
12. Clostridium perfringens virulence factors are nonredundant activators of the NLRP3 inflammasome.
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Mathur, Anukriti, Kay, Callum, Xue, Yansong, Pandey, Abhimanu, Lee, Jiwon, Jing, Weidong, Enosi Tuipulotu, Daniel, Lo Pilato, Jordan, Feng, Shouya, Ngo, Chinh, Zhao, Anyang, Shen, Cheng, Rug, Melanie, Miosge, Lisa A, Atmosukarto, Ines I, Price, Jason D, Ali, Sidra A, Gardiner, Elizabeth E, Robertson, Avril AB, and Awad, Milena M
- Abstract
Inflammasome signaling is a central pillar of innate immunity triggering inflammation and cell death in response to microbes and danger signals. Here, we show that two virulence factors from the human bacterial pathogen Clostridium perfringens are nonredundant activators of the NLRP3 inflammasome in mice and humans. C. perfringens lecithinase (also known as phospolipase C) and C. perfringens perfringolysin O induce distinct mechanisms of activation. Lecithinase enters LAMP1+ vesicular structures and induces lysosomal membrane destabilization. Furthermore, lecithinase induces the release of the inflammasome‐dependent cytokines IL‐1β and IL‐18, and the induction of cell death independently of the pore‐forming proteins gasdermin D, MLKL and the cell death effector protein ninjurin‐1 or NINJ1. We also show that lecithinase triggers inflammation via the NLRP3 inflammasome in vivo and that pharmacological blockade of NLRP3 using MCC950 partially prevents lecithinase‐induced lethality. Together, these findings reveal that lecithinase activates an alternative pathway to induce inflammation during C. perfringens infection and that this mode of action can be similarly exploited for sensing by a single inflammasome. Synopsis: Clostridium perfringens causes gas gangrene and clostridial myonecrosis in humans. This study shows that the secreted C. perfringens toxins lecithinase and perfringolysin O activate the NLRP3 inflammasome via distinct mechanisms. Perfringolysin O (PFO) is a pore‐forming toxin that mediates K+ efflux at the plasma membrane.Lecithinase is a phospholipase that is endocytosed and triggers lysosomal membrane destabilization.Lecithinase is the first example of a microbial phospholipase that activates the mammalian innate immune system.Lecithinase‐driven inflammasome responses do not require GSDMD and NINJ1. [ABSTRACT FROM AUTHOR]
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- 2023
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13. Clostridial Gas Gangrene: Evidence That α and θ Toxins Differentially Modulate the Immune Response and Induce Acute Tissue Necrosis
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Stevens, Dennis L., Tweten, Rodney K., Awad, Milena M., Rood, Julian I., and Bryant, Amy E.
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- 1997
14. Clostridium septicum α-toxin activates the NLRP3 inflammasome by engaging GPI-anchored proteins.
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Jing, Weidong, Pilato, Jordan Lo, Kay, Callum, Feng, Shouya, Tuipulotu, Daniel Enosi, Mathur, Anukriti, Shen, Cheng, Ngo, Chinh, Zhao, Anyang, Miosge, Lisa A., Ali, Sidra A., Gardiner, Elizabeth E., Awad, Milena M., Lyras, Dena, Robertson, Avril A. B., Kaakoush, Nadeem O., and Man, Si Ming
- Abstract
Clostridium species are a group of Gram-positive bacteria that cause diseases in humans, such as food poisoning, botulism, and tetanus. Here, we analyzed 10 different Clostridium species and identified that Clostridium septicum, a pathogen that causes sepsis and gas gangrene, activates the mammalian cytosolic inflammasome complex in mice and humans. Mechanistically, we demonstrate that α-toxin secreted by C. septicum binds to glycosylphosphatidylinositol (GPI)–anchored proteins on the host plasma membrane, oligomerizing and forming a membrane pore that is permissive to efflux of magnesium and potassium ions. Efflux of these cytosolic ions triggers the activation of the innate immune sensor NLRP3, inducing activation of caspase-1 and gasdermin D, secretion of the proinflammatory cytokines interleukin-1β and interleukin-18, pyroptosis, and plasma membrane rupture via ninjurin-1. Furthermore, α-toxin of C. septicum induces rapid inflammasome-mediated lethality in mice and pharmacological inhibition of the NLRP3 inflammasome using MCC950 prevents C. septicum–induced lethality. Overall, our results reveal that cytosolic innate sensing of α-toxin is central to the recognition of C. septicum infection and that therapeutic blockade of the inflammasome pathway may prevent sepsis and death caused by toxin-producing pathogens. Toxin-triggered tribulations: Lurking within the resident human intestinal microbiota are Clostridium bacteria with the capacity to cause rare but serious deep tissue infections like gas gangrene after hematogenous dissemination. Jing et al. analyzed the downstream cellular signaling pathways triggered when Clostridium septicum α-toxin bound to the plasma membrane surface, triggering pore formation followed by potassium and magnesium ion efflux. The α-toxin–induced ion fluxes activated NLRP3 inflammasomes, leading to gasdermin D–dependent pore formation and cell death by pyroptosis that could be blocked by the NLRP3-specific inhibitor MCC950. These findings indicate that pharmacological inhibition of the NLRP3 inflammasome may provide a new option for the treatment of life-threatening C. septicum infections. [ABSTRACT FROM AUTHOR]
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- 2022
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15. Perfringolysin O expression in Clostridium perfringens is independent of the upstream pfoR gene
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Awad, Milena M. and Rood, Julian I.
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Pathogenic microorganisms -- Genetic aspects ,Gene expression -- Analysis ,Genetic transcription -- Regulation ,Biological sciences - Abstract
Results point out that the Clostridium perfringens pfoR gene encoded transcriptional activator is not likely to play a role in the activation of the pfoA gene expression. This is corroborated by a mutation in pfoR not altering perfringolysin O production in the bacterrium.
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- 2002
16. TcdB or not TcdB: a tale of two Clostridium difficile toxins
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Carter, Glen P, Awad, Milena M, Kelly, Michelle L, Rood, Julian I, and Lyras, Dena
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- 2011
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17. The α-toxin of Clostridium septicum is essential for virulence
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Kennedy, Catherine L., Krejany, Efrosinia O., Young, Lauren F., OʼConnor, Jennifer R., Awad, Milena M., Boyd, Richard L., Emmins, John J., Lyras, Dena, and Rood, Julian I.
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- 2005
18. PGFinder, a novel analysis pipeline for the consistent, reproducible, and high-resolution structural analysis of bacterial peptidoglycans.
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Patel, Ankur V., Turner, Robert D., Rifflet, Aline, Acosta-Martin, Adelina E., Nichols, Andrew, Awad, Milena M., Lyras, Dena, Boneca, Ivo Gomperts, Bern, Marshall, Collins, Mark O., and Mesnage, Stéphane
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- 2021
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19. A dynamic, ring-forming MucB / RseB-like protein influences spore shape in Bacillus subtilis.
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Luhur, Johana, Chan, Helena, Kachappilly, Benson, Mohamed, Ahmed, Morlot, Cécile, Awad, Milena, Lyras, Dena, Taib, Najwa, Gribaldo, Simonetta, Rudner, David Z., and Rodrigues, Christopher D. A.
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BACILLUS subtilis ,SPOREFORMING bacteria ,SPORES ,STEM cells ,FUNGAL spores ,PROTEIN domains ,TRANSMISSION electron microscopy ,BACTERIAL inactivation - Abstract
How organisms develop into specific shapes is a central question in biology. The maintenance of bacterial shape is connected to the assembly and remodelling of the cell envelope. In endospore-forming bacteria, the pre-spore compartment (the forespore) undergoes morphological changes that result in a spore of defined shape, with a complex, multi-layered cell envelope. However, the mechanisms that govern spore shape remain poorly understood. Here, using a combination of fluorescence microscopy, quantitative image analysis, molecular genetics and transmission electron microscopy, we show that SsdC (formerly YdcC), a poorly-characterized new member of the MucB / RseB family of proteins that bind lipopolysaccharide in diderm bacteria, influences spore shape in the monoderm Bacillus subtilis. Sporulating cells lacking SsdC fail to adopt the typical oblong shape of wild-type forespores and are instead rounder. 2D and 3D-fluorescence microscopy suggest that SsdC forms a discontinuous, dynamic ring-like structure in the peripheral membrane of the mother cell, near the mother cell proximal pole of the forespore. A synthetic sporulation screen identified genetic relationships between ssdC and genes involved in the assembly of the spore coat. Phenotypic characterization of these mutants revealed that spore shape, and SsdC localization, depend on the coat basement layer proteins SpoVM and SpoIVA, the encasement protein SpoVID and the inner coat protein SafA. Importantly, we found that the ΔssdC mutant produces spores with an abnormal-looking cortex, and abolishing cortex synthesis in the mutant largely supresses its shape defects. Thus, SsdC appears to play a role in the proper assembly of the spore cortex, through connections to the spore coat. Collectively, our data suggest functional diversification of the MucB / RseB protein domain between diderm and monoderm bacteria and identify SsdC as an important factor in spore shape development. Author summary: Cell shape is an important cellular attribute linked to cellular function and environmental adaptation. Bacterial endospores are one of the toughest cell types on Earth, with a defined shape and complex, highly-resistant, multi-layered cell envelope. Although decades of research have focused on defining the composition and assembly of the multi-layered spore envelope, little is known about how these layers contribute to spore shape. Here, we identify SsdC, a poorly-characterized new member of the MucB / RseB family of proteins that bind lipopolysaccharide in diderm bacteria. We show that SsdC is an important factor in spore shape development in the monoderm, model organism Bacillus subtilis. Our data suggest that SsdC influences the assembly of the spore cortex, through connections to the spore coat, by forming an intriguing, dynamic ring-like structure adjacent to the developing spore. Furthermore, our identification of SsdC suggests evolutionary diversification of the MucB /RseB protein domain between diderm and monoderm bacteria. [ABSTRACT FROM AUTHOR]
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- 2020
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20. Paeniclostridium (Clostridium) sordellii–associated enterocolitis in 7 horses.
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Uzal, Francisco A., Navarro, Mauricio A., Hostetter, Jesse M., Nyaoke, Akinyi C., Fresneda, Karina, Diab, Santiago S., Moore, Janet, Lyras, Dena, and Awad, Milena
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ENTEROCOLITIS ,HORSE diseases ,HORSES ,ANIMAL diseases ,CLOSTRIDIUM ,INTESTINAL infections ,CLOSTRIDIA - Abstract
Enteric disease in horses may be caused by a variety of microorganisms, including several clostridial species. Paeniclostridium sordellii (previously Clostridium sordellii) has been frequently associated with gas gangrene in humans and several animal species, including horses. However, its role in enteric diseases of animals has not been fully determined. We describe herein 7 cases of enteric disease in horses associated with P. sordellii infection. Grossly, the small and/or large intestines were necrotic, hemorrhagic, and edematous. Microscopically, there was severe mucosal necrosis and hemorrhage of the small and/or large intestine of all horses. P. sordellii was isolated and/or demonstrated by immunohistochemistry and/or PCR in the intestine of all horses. All other known causes of enteric disease in horses were ruled out in these 7 cases. P. sordellii should be considered among the differential diagnoses in cases of enteric disease in horses. [ABSTRACT FROM AUTHOR]
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- 2020
- Full Text
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21. Tranexamic Acid Influences the Immune Response, but not Bacterial Clearance in a Model of Post-Traumatic Brain Injury Pneumonia.
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Draxler, Dominik F., Awad, Milena M., Hanafi, Gryselda, Daglas, Maria, Ho, Heidi, Keragala, Charithani, Galle, Adam, Roquilly, Antoine, Lyras, Dena, Sashindranath, Maithili, and Medcalf, Robert L.
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TRANEXAMIC acid , *LEUKOCYTE count , *IMMUNE response , *BRAIN injuries , *PNEUMONIA , *LIPOTEICHOIC acid - Abstract
The antifibrinolytic agent, tranexamic acid (TXA), an inhibitor of plasmin formation, currently is evaluated to reduce bleeding in various conditions, including traumatic brain injury (TBI). Because plasmin is implicated in inflammation and immunity, we investigated the effects of plasmin inhibition on the immune response after TBI in the presence or absence of induced pneumonia. Wild-type mice treated with vehicle or TXA or mice deficient in plasminogen (plg-/-) underwent TBI using the controlled cortical impact model. Mice were then subjected to Staphylococcus aureus induced pneumonia and the degree of immune competence determined. Significant baseline changes in the innate immune cell profile were seen in plg-/- mice with increases in spleen weight and white blood cell counts, and elevation in plasma interleukin-6 levels. The plg-/- mice subjected to TBI displayed no additional changes in these parameters at the 72 h or one week time point post-TBI. The plg-/- mice subjected to TBI did not exhibit any further increase in susceptibility to endogenous infection. Pneumonia was induced by intratracheal instillation of S. aureus. The TBI did not worsen pneumonia symptoms or delay recovery in plg-/- mice. Similarly, in wild type mice, treatment with TXA did not impact on the ability of mice to counteract pneumonia after TBI. Administration of TXA after TBI and subsequent pneumonia, however, altered the number and surface marker expression of several myeloid and lymphoid cell populations, consistent with enhanced immune activation at the 72 h time point. This investigation confirms the immune-modulatory properties of TXA, thereby highlighting its effects unrelated to inhibition of fibrinolysis. [ABSTRACT FROM AUTHOR]
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- 2019
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22. Clostridium sordellii outer spore proteins maintain spore structural integrity and promote bacterial clearance from the gastrointestinal tract.
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Rabi, Rebecca, Larcombe, Sarah, Mathias, Rommel, McGowan, Sheena, Awad, Milena, and Lyras, Dena
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CLOSTRIDIUM ,BACTERIAL diseases ,GASTROINTESTINAL diseases ,TOXIC shock syndrome ,SEPTIC shock - Abstract
Bacterial spores play an important role in disease initiation, transmission and persistence. In some species, the exosporium forms the outermost structure of the spore and provides the first point of contact between the spore and the environment. The exosporium may also be involved in spore adherence, protection and germination. Clostridium sordellii is a highly lethal, spore forming pathogen that causes soft-tissue infections, enteritis and toxic-shock syndrome. Despite the importance of C. sordellii spores in disease, spore proteins from this bacterium have not been defined or interrogated functionally. In this study, we identified the C. sordellii outer spore proteome and two of the identified proteins, CsA and CsB, were characterised using a genetic and phenotypic approach. Both proteins were essential for the correct formation and positioning of the C. sordellii spore coat and exosporium. The absence of CsA reduced sporulation levels and increased spore sensitivity to heat, sodium hydroxide and hydrochloric acid. By comparison, CsB was required for normal levels of spore adherence to cervical, but not vaginal, cells, with csB mutant spores having increased adherence properties. The establishment of a mouse infection model of the gastrointestinal tract for C. sordellii allowed the role of CsA and CsB to be interrogated in an infected host. Following the oral administration of spores to mice, the wild-type strain efficiently colonized the gastrointestinal tract, with the peak of bacterial numbers occurring at one day post-infection. Colonization was reduced by two logs at four days post-infection. By comparison, mice infected with the csB mutant did not show a reduction in bacterial numbers. We conclude that C. sordellii outer spore proteins are important for the structural and functional integrity of spores. Furthermore, outer spore proteins are required for wild-type levels of colonization during infection, possibly as a result of the role that the proteins play in spore structure and morphology. [ABSTRACT FROM AUTHOR]
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- 2018
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23. Structural Characterization of Clostridium sordellii Spores of Diverse Human, Animal, and Environmental Origin and Comparison to Clostridium difficile Spores.
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Rabi, Rebecca, Turnbull, Lynne, Whitchurch, Cynthia B., Awad, Milena, and Lyras, Dena
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- 2017
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24. The NEAT Domain-Containing Proteins of Clostridium perfringens Bind Heme.
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Choo, Jocelyn M., Cheung, Jackie K., Wisniewski, Jessica A., Steer, David L., Bulach, Dieter M., Hiscox, Thomas J., Chakravorty, Anjana, Smith, A. Ian, Gell, David A., Rood, Julian I., and Awad, Milena M.
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PATHOGENIC bacteria ,CLOSTRIDIUM perfringens ,VIRULENCE of bacteria ,POST-translational modification ,BACTERIAL growth ,BACTERIAL genomes - Abstract
The ability of a pathogenic bacterium to scavenge iron from its host is important for its growth and survival during an infection. Our studies on C. perfringens gas gangrene strain JIR325, a derivative of strain 13, showed that it is capable of utilizing both human hemoglobin and ferric chloride, but not human holo-transferrin, as an iron source for in vitro growth. Analysis of the C. perfringens strain 13 genome sequence identified a putative heme acquisition system encoded by an iron-regulated surface gene region that we have named the Cht (Clostridium perfringens heme transport) locus. This locus comprises eight genes that are co-transcribed and includes genes that encode NEAT domain-containing proteins (ChtD and ChtE) and a putative sortase (Srt). The ChtD, ChtE and Srt proteins were shown to be expressed in JIR325 cells grown under iron-limited conditions and were localized to the cell envelope. Moreover, the NEAT proteins, ChtD and ChtE, were found to bind heme. Both chtDE and srt mutants were constructed, but these mutants were not defective in hemoglobin or ferric chloride utilization. They were, however, attenuated for virulence when tested in a mouse myonecrosis model, although the virulence phenotype could not be restored via complementation and, as is common with such systems, secondary mutations were identified in these strains. In summary, this study provides evidence for the functional redundancies that occur in the heme transport pathways of this life threatening pathogen. [ABSTRACT FROM AUTHOR]
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- 2016
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25. The Sialidase NanS Enhances Non-TcsL Mediated Cytotoxicity of Clostridium sordellii.
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Awad, Milena M., Singleton, Julie, and Lyras, Dena
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The clostridia produce an arsenal of toxins to facilitate their survival within the host environment. TcsL is one of two major toxins produced by Clostridium sordellii, a human and animal pathogen, and is essential for disease pathogenesis of this bacterium. C. sordellii produces many other toxins, but the role that they play in disease is not known, although previous work has suggested that the sialidase enzyme NanS may be involved in the characteristic leukemoid reaction that occurs during severe disease. In this study we investigated the role of NanS in C. sordellii disease pathogenesis. We constructed a nanS mutant and showed that NanS is the only sialidase produced from C. sordellii strain ATCC9714 since sialidase activity could not be detected from the nanS mutant. Complementation with the wild-type gene restored sialidase production to the nanS mutant strain. Cytotoxicity assays using sialidase-enriched culture supernatants applied to gut (Caco2), vaginal (VK2), and cervical cell lines (End1/E6E7 and Ect1/E6E7) showed that NanS was not cytotoxic to these cells. However, the cytotoxic capacity of a toxin-enriched supernatant to the vaginal and cervical cell lines was substantially enhanced in the presence of NanS. TcsL was not the mediator of the observed cytotoxicity since supernatants harvested from a TcsL-deficient strain displayed similar cytotoxicity levels to TcsL-containing supernatants. This study suggests that NanS works synergistically with an unknown toxin or toxins to exacerbate C. sordellii-mediated tissue damage in the host. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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26. Disruption of the Gut Microbiome: Clostridium difficile Infection and the Threat of Antibiotic Resistance.
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Johanesen, Priscilla A., Mackin, Kate E., Hutton, Melanie L., Awad, Milena M., Larcombe, Sarah, Amy, Jacob M., and Lyras, Dena
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CLOSTRIDIOIDES difficile ,HUMAN microbiota ,GUT microbiome ,DRUG resistance in bacteria ,MOBILE genetic elements ,PATHOLOGICAL physiology ,PHYSIOLOGICAL effects of antibiotics - Abstract
Clostridium difficile is well recognized as the leading cause of antibiotic-associated diarrhea, having a significant impact in both health-care and community settings. Central to predisposition to C. difficile infection is disruption of the gut microbiome by antibiotics. Being a Gram-positive anaerobe, C. difficile is intrinsically resistant to a number of antibiotics. Mobile elements encoding antibiotic resistance determinants have also been characterized in this pathogen. While resistance to antibiotics currently used to treat C. difficile infection has not yet been detected, it may be only a matter of time before this occurs, as has been seen with other bacterial pathogens. This review will discuss C. difficile disease pathogenesis, the impact of antibiotic use on inducing disease susceptibility, and the role of antibiotic resistance and mobile elements in C. difficile epidemiology. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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27. Clostridium sordellii genome analysis reveals plasmid localized toxin genes encoded within pathogenicity loci.
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Couchman, Edward C., Browne, Hilary P., Dunn, Matt, Lawley, Trevor D., Songer, J. Glenn, Hall, Val, Petrovska, Liljana, Vidor, Callum, Awad, Milena, Lyras, Dena, and Fairweather, Neil F.
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CLOSTRIDIOIDES difficile ,CLOSTRIDIUM novyi ,CLOSTRIDIUM perfringens ,PLASMIDS ,MICROBIAL virulence ,VIRULENCE of bacteria ,PATHOGENIC microorganisms - Abstract
Background: Clostridium sordellii can cause severe infections in animals and humans, the latter associated with trauma, toxic shock and often-fatal gynaecological infections. Strains can produce two large clostridial cytotoxins (LCCs), TcsL and TcsH, related to those produced by Clostridium difficile, Clostridium novyi and Clostridium perfringens, but the genetic basis of toxin production remains uncharacterised. Results: Phylogenetic analysis of the genome sequences of 44 strains isolated from human and animal infections in the UK, US and Australia placed the species into four clades. Although all strains originated from animal or clinical disease, only 5 strains contained LCC genes: 4 strains contain tcsL alone and one strain contains tcsL and tcsH. Four toxin-positive strains were found within one clade. Where present, tcsL and tcsH were localised in a pathogenicity locus, similar to but distinct from that present in C. difficile. In contrast to C. difficile, where the LCCs are chromosomally localised, the C. sordellii tcsL and tcsH genes are localised on plasmids. Our data suggest gain and loss of entire toxigenic plasmids in addition to horizontal transfer of the pathogenicity locus. A high quality, annotated sequence of ATCC9714 reveals many putative virulence factors including neuraminidase, phospholipase C and the cholesterol-dependent cytolysin sordellilysin that are highly conserved between all strains studied. Conclusions: Genome analysis of C. sordellii reveals that the LCCs, the major virulence factors, are localised on plasmids. Many strains do not contain the LCC genes; it is probable that in several of these cases the plasmid has been lost upon laboratory subculture. Our data are consistent with LCCs being the primary virulence factors in the majority of infections, but LCC-negative strains may precipitate certain categories of infection. A high quality genome sequence reveals putative virulence factors whose role in virulence can be investigated. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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28. The Pore-Forming α-Toxin from Clostridium septicum Activates the MAPK Pathway in a Ras-c-Raf-Dependent and Independent Manner.
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Chakravorty, Anjana, Awad, Milena M., Cheung, Jackie K., Hiscox, Thomas J., Lyras, Dena, and Rood, Julian I.
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CLOSTRIDIUM , *GAS gangrene , *MOLECULAR immune response , *DUAL specificity phosphatase 1 , *EXTRACELLULAR signal-regulated kinases , *LABORATORY mice - Abstract
Clostridium septicum is the causative agent of atraumatic gas gangrene, with α-toxin, an extracellular pore-forming toxin, essential for disease. How C. septicum modulates the host's innate immune response is poorly defined, although α-toxin-intoxicated muscle cells undergo cellular oncosis, characterised by mitochondrial dysfunction and release of reactive oxygen species. Nonetheless, the signalling events that occur prior to the initiation of oncosis are poorly characterised. Our aims were to characterise the ability of α-toxin to activate the host mitogen activated protein kinase (MAPK) signalling pathway both in vitro and in vivo. Treatment of Vero cells with purified α-toxin activated the extracellular-signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 arms of the MAPK pathway and stimulated the release of TNF-α in a dose-dependent manner. Studies using inhibitors of all three MAPK components suggested that activation of ERK occurred in a Ras-c-Raf dependent manner, whereas activation of JNK and p38 occurred by a Ras-independent mechanism. Toxin-mediated activation was dependent on efficient receptor binding and pore formation and on an influx of extracellular calcium ions. In the mouse myonecrosis model we showed that the MAPK pathway was activated in tissues of infected mice, implying that it has an important role in the disease process. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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29. Clostridium difficile virulence factors: Insights into an anaerobic spore-forming pathogen.
- Author
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Awad, Milena M, Johanesen, Priscilla A, Carter, Glen P, Rose, Edward, and Lyras, Dena
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- 2014
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30. A Highly Specific Holin-Mediated Mechanism Facilitates the Secretion of Lethal Toxin TcsL in Paeniclostridium sordellii.
- Author
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Vidor, Callum J., Hamiot, Audrey, Wisniewski, Jessica, Mathias, Rommel A., Dupuy, Bruno, Awad, Milena, and Lyras, Dena
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CLOSTRIDIUM perfringens ,CLOSTRIDIOIDES difficile ,TOXINS ,SECRETION ,PROTEOMICS ,CLOSTRIDIA - Abstract
Protein secretion is generally mediated by a series of distinct pathways in bacteria. Recently, evidence of a novel bacterial secretion pathway involving a bacteriophage-related protein has emerged. TcdE, a holin-like protein encoded by toxigenic isolates of Clostridioides difficile, mediates the release of the large clostridial glucosylating toxins (LCGTs), TcdA and TcdB, and TpeL from C. perfringens uses another holin-like protein, TpeE, for its secretion; however, it is not yet known if TcdE or TpeE secretion is specific to these proteins. It is also unknown if other members of the LCGT-producing clostridia, including Paeniclostridium sordellii (previously Clostridium sordellii), use a similar toxin-release mechanism. Here, we confirm that each of the LCGT-producing clostridia encode functional holin-like proteins in close proximity to the toxin genes. To characterise the respective roles of these holin-like proteins in the release of the LCGTs, P. sordellii and its lethal toxin, TcsL, were used as a model. Construction and analysis of mutants of the P. sordellii tcsE (holin-like) gene demonstrated that TcsE plays a significant role in TcsL release. Proteomic analysis of the secretome from the tcsE mutant confirmed that TcsE is required for efficient TcsL secretion. Unexpectedly, comparative sample analysis showed that TcsL was the only protein significantly altered in its release, suggesting that this holin-like protein has specifically evolved to function in the release of this important virulence factor. This specificity has, to our knowledge, not been previously shown and suggests that this protein may function as part of a specific mechanism for the release of all LCGTs. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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31. The Cysteine Protease &agr;-Clostripain is Not Essential for the Pathogenesis of Clostridium perfringens-Mediated Myonecrosis.
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Chakravorty, Anjana, Awad, Milena M., Hiscox, Thomas J., Cheung, Jackie K., Carter, Glen P., Choo, Jocelyn M., Lyras, Dena, and Rood, Julian I.
- Subjects
- *
CYSTEINE proteinases , *CLOSTRIDIUM perfringens , *NECROSIS , *CLOSTRIDIUM diseases , *SKIM milk , *EXTRACELLULAR enzymes , *PROTEOLYSIS , *DISEASE progression - Abstract
Clostridium perfringens is the causative agent of clostridial myonecrosis or gas gangrene and produces many different extracellular toxins and enzymes, including the cysteine protease &agr;-clostripain. Mutation of the a-clostripain structural gene, ccp, alters the turnover of secreted extracellular proteins in C. perfringens, but the role of &agr;-clostripain in disease pathogenesis is not known. We insertionally inactivated the ccp gene C. perfringens strain 13 using TargeTron technology, constructing a strain that was no longer proteolytic on skim milk agar. Quantitative protease assays confirmed the absence of extracellular protease activity, which was restored by complementation with the wild-type ccp gene. The role of aclostripain in virulence was assessed by analysing the isogenic wild-type, mutant and complemented strains in a mouse myonecrosis model. The results showed that although &agr;-clostripain was the major extracellular protease, mutation of the ccp gene did not alter either the progression or the development of disease. These results do not rule out the possibility that this extracellular enzyme may still have a role in the early stages of the disease process. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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32. Functional Analysis of the VirSR Phosphorelay from Clostridium perfringens.
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Cheung, Jackie K., Awad, Milena M., McGowan, Sheena, and Rood, Julian I.
- Subjects
- *
CLOSTRIDIUM perfringens , *TOXINS , *CLOSTRIDIUM , *GENETIC transduction , *HISTIDINE , *PHOSPHORYLATION , *MUTAGENESIS , *CHROMOSOMES , *GENETIC mutation - Abstract
Toxin production in Clostridium perfringens is controlled by the VirSR two-component signal transduction system, which comprises the VirS sensor histidine kinase and the VirR response regulator. Other studies have concentrated on the elucidation of the genes controlled by this network; there is little information regarding the phosphorelay cascade that is the hallmark of such regulatory systems. In this study, we have examined each step in this cascade, beginning with autophosphorylation of VirS, followed by phosphotransfer from VirS to VirR. We also have studied the effects of gene dosage and phosphorylation in vivo. We have used random and site-directed mutagenesis to identify residues in VirS that are important for its function and have identified a region in the putative sensory domain of VirS that appeared to be essential for function. In vitro phosphorylation studies showed that VirSc, a truncated VirS protein that lacked the N-terminal sensory domain, was capable of autophosphorylation and could subsequently act as a phosphodonor for its cognate response regulator, VirR. Conserved residues of both VirS and VirR, including the D57 residue of VirR, were shown to be essential for this process. By use of Targetron technology, we were able to introduce a single copy of virR or virRD57N onto the chromosome of a virR mutant of C. perfringens. The results showed that in vivo, when virR was present in single copy, the production of wild-type levels of perfringolysin O was dependent on the presence of virS and an unaltered D57 residue in VirR. These results provide good evidence that phosphorylation is critical for VirR function. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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33. Molecular and Cellular Basis of Microvascular Perfusion Deficits Induced by Clostridium perfringens and Clostridium septicum.
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Hickey, Michael J., Kwan, Rain Y. Q., Awad, Milena M., Kennedy, Catherine L., Young, Lauren F., Hall, Pam, Cordner, Leanne M., Lyras, Dena, Emmins, John J., and Rood, Julian I.
- Subjects
PERFUSION ,CLOSTRIDIUM perfringens ,CLOSTRIDIUM diseases ,TETANUS toxin ,GAS gangrene ,BACTERIAL antitoxins - Abstract
Reduced tissue perfusion leading to tissue ischemia is a central component of the pathogenesis of myonecrosis caused by Clostridium perfringens. The C. perfringens α-toxin has been shown capable of inducing these changes, but its potential synergy with perfringolysin O (θ-toxin) is less well understood. Similarly, Clostridium septicum is a highly virulent causative agent of spontaneous gas gangrene, but its effect on the microcirculation has not been examined. Therefore, the aim of this study was to use intravital microscopy to examine the effects of C. perfringens and C. septicum on the functional microcirculation, coupled with the use of isogenic toxin mutants to elucidate the role of particular toxins in the resultant microvascular perfusion deficits. This study represents the first time this integrated approach has been used in the analysis of the pathological response to clostridial toxins. Culture supernatants from wild-type C. perfringens induced extensive cell death within 30 min, as assessed by in vivo uptake of propidium iodide. Furthermore, significant reductions in capillary perfusion were observed within 60 min. Depletion of either platelets or neutrophils reduced the alteration in perfusion, consistent with a role for these blood-borne cells in obstructing perfusion. In addition, mutation of either the α-toxin or perfringolysin O structural genes attenuated the reduction in perfusion, a process that was reversed by genetic complementation. C. septicum also induced a marked reduction in perfusion, with the degree of microvascular compromise correlating with the level of the C. septicum α-toxin. Together, these data indicate that as a result of its ability to produce atoxin and perfringolysin O, C. perfringens rapidly induces irreversible cellular injury and a marked reduction in microvascular perfusion. Since C. septicum induces a similar reduction in microvascular perfusion, it is postulated that this function is central to the pathogenesis of clostridial myonecrosis, irrespective of the causative bacterium. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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34. Virulence studies on chromosomal α--toxin and 0--toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of α--toxin in Clostridium perfringens--mediated gas gangrene.
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Awad, Milena M., Bryant, Amy E., Stevens, Dennis L., and Rood, Julian I.
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MICROBIAL virulence ,PATHOGENIC microorganisms ,CLOSTRIDIUM perfringens ,PHOTOSYNTHETIC oxygen evolution ,CYTOPLASMIC inheritance ,GAS gangrene - Abstract
The pathogenesis of clostridial myonecrosis, or gas gangrene, involves the growth of the anaerobic bacterium Clostridium perfringens in the infected tissues and the elaboration of numerous extracellular toxins and enzymes. The precise role of each of these toxins in tissue invasion and necrosis has not been determined. To enable genetic approaches to be used to study C. perfringens pathogenesis we developed an Allelic exchange method which involved the transformation of C. perfringens cells with a suicide plasmid carrying a gene insertionally inactivated with an erythromycin-resistance determinant. The frequency with which double reciprocal cross-over events were observed was increased to a workable level by increasing the amount of homologous DNA located on either side of the inactivated gene. Allelic exchange was used to isolate mutations in the 'chromosomal pfoA gene, which encodes an oxygen-labile haemolysin known as θ-toxin or perfringolysin O. and in the chromosomal plc gene, which encodes the α-toxin or phospholipase C. The resultant mutants failed to produce detectable θ-toxin or θ-toxin activity, respectively, and could be complemented by recombinant plasmids that carried the respective wild-type genes. The resultant strains were virulence tested in a mouse myonecrosis model. The results showed that the plc mutants had demonstrably reduced virulence and therefore provided definitive genetic evidence tor the essential role of α-toxin in gas gangrene or clostridial myonecrosis. [ABSTRACT FROM AUTHOR]
- Published
- 1995
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35. Identification and molecular analysis of a locus that regulates extracellular toxin production in <em>Clostridium perfringens</em>.
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Lyristis, Michael, Bryant, Amy E., Sloan, Joan, Awad, Milena M., Nisbet, Ian T., Stevens, Dennis L., and Rood, Julian I.
- Subjects
ANAEROBIC bacteria ,CLOSTRIDIUM perfringens ,GAS gangrene ,TRANSPOSONS ,MUTAGENESIS - Abstract
The anaerobic bacterium Clostridium perfringens mediates clostridial myonecrosis. or gas gangrene, by producing a number of extracellular toxins and enzymes. Transposon mutagenesis with Tn916 was used to isolate a pleiotropic mutant of C. perfringens that produced reduced levels of phospholipase C, protease and sialidase, and did not produce any detectable perfringolysin O activity. Southern hybridization revealed that a single copy of Tn916 had inserted into a 2.7 kb Hindlll fragment in the C. perfringens chromosome. A 4.3 kb Pstl fragment, which spanned the Tn916 insertion site, was cloned from the wild-type strain. When subcloned into a shuttle vector and introduced into C. perfringens this fragment was able to complement the Tn916-derived mutation. Transformation of the mutant with plasmids containing the 2.7 kb HindllL fragment, or the 4.3 kb Pstl fragment, resulted In toxin and enzyme levels greater than or equal to those of the wild-type strain. The Pstl fragment was sequenced and found to potentially encode seven open reading frames, two of which appeared to be arranged in an operon and shared sequence similarity with members of two-component signal transduction systems. The putative virR gene encoded a protein with a deduced molecular weight of 30140, and with sequence similarity to activators in the response regulator family of proteins. The next gene, virS, into which Tn916 had inserted, was predicted to encode a membrane-spanning protein with a deduced molecular weight of 51 274. The putative VirS protein had sequence similarity to sensor proteins and also contained a histidine residue highly conserved in the histidine protein kinase family of sensor proteins. Virulence studies carried out using a mouse model implicated the virS gene in the pathogenesis of histotoxic C. perfringens infections. It was concluded that a two-component... [ABSTRACT FROM AUTHOR]
- Published
- 1994
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36. Human Plasminogen Exacerbates Clostridioides difficile Enteric Disease and Alters the Spore Surface.
- Author
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Awad, Milena M., Hutton, Melanie L., Quek, Adam J., Klare, William P., Mileto, Steven J., Mackin, Kate, Ly, Diane, Oorschot, Viola, Bosnjak, Marijana, Jenkin, Grant, Conroy, Paul J., West, Nick, Fulcher, Alex, Costin, Adam, Day, Christopher J., Jennings, Michael P., Medcalf, Robert L., Sanderson-Smith, Martina, Cordwell, Stuart J., and Law, Ruby H.P.
- Abstract
The protease plasmin is an important wound healing factor, but it is not clear how it affects gastrointestinal infection–mediated damage, such as that resulting from Clostridioides difficile. We investigated the role of plasmin in C difficile –associated disease. This bacterium produces a spore form that is required for infection, so we also investigated the effects of plasmin on spores. C57BL/6J mice expressing the precursor to plasmin, the zymogen human plasminogen (hPLG), or infused with hPLG were infected with C difficile , and disease progression was monitored. Gut tissues were collected, and cytokine production and tissue damage were analyzed by using proteomic and cytokine arrays. Antibodies that inhibit either hPLG activation or plasmin activity were developed and structurally characterized, and their effects were tested in mice. Spores were isolated from infected patients or mice and visualized using super-resolution microscopy; the functional consequences of hPLG binding to spores were determined. hPLG localized to the toxin-damaged gut, resulting in immune dysregulation with an increased abundance of cytokines (such as interleukin [IL] 1A, IL1B, IL3, IL10, IL12B, MCP1, MP1A, MP1B, GCSF, GMCSF, KC, TIMP-1), tissue degradation, and reduced survival. Administration of antibodies that inhibit plasminogen activation reduced disease severity in mice. C difficile spores bound specifically to hPLG and active plasmin degraded their surface, facilitating rapid germination. We found that hPLG is recruited to the damaged gut, exacerbating C difficile disease in mice. hPLG binds to C difficile spores, and, upon activation to plasmin, remodels the spore surface, facilitating rapid spore germination. Inhibitors of plasminogen activation might be developed for treatment of C difficile or other infection-mediated gastrointestinal diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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37. pCP13, a representative of a new family of conjugative toxin plasmids in Clostridium perfringens.
- Author
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Watts, Thomas D., Vidor, Callum J., Awad, Milena M., Lyras, Dena, Rood, Julian I., and Adams, Vicki
- Subjects
- *
PLASMIDS , *CLOSTRIDIUM perfringens , *TOXINS , *CLOSTRIDIUM botulinum , *CLOSTRIDIOIDES difficile , *FAMILIES , *ENTEROTOXINS - Abstract
Conjugative transfer is a major contributor to the dissemination of antibiotic resistance and virulence genes in the human and animal pathogen, Clostridium perfringens. The C. perfringens plasmid pCW3 is the archetype of an extensive family of highly related conjugative toxin and antibiotic resistance plasmids found in this bacterium. These plasmids were thought to constitute the only conjugative plasmid family in C. perfringens. Recently, another series of C. perfringens plasmids, the pCP13-like family, have been shown to harbour important toxin genes, including genes that encode the novel binary clostridial enterotoxin, BEC. Based on early bioinformatics analysis this plasmid family was thought to be non-conjugative. Here we demonstrate that pCP13 is in fact conjugative, transfers at high frequency and that the newly defined Pcp conjugation locus encodes putative homologues of a type 4 secretion system (T4SS), one of which, PcpB4, was shown to be essential for transfer. The T4SS of pCP13 also appears to be evolutionarily related to conjugative toxin plasmids from other clostridia-like species, including Paeniclostridium (formerly Clostridium) sordellii , Clostridioides (formerly Clostridium) difficile and Clostridium botulinum. Therefore, it is clear that there are two distinct families of conjugative plasmids in C. perfringens : the pCW3 family and the pCP13 family. This study has significant implications for our understanding of the movement of toxin genes both within C. perfringens , but also potentially to other pathogenic clostridia. • Members of the pCW3 family were thought to be the only conjugative plasmids in C. perfringens. • We demonstrate that pCP13 represents a new family of conjugative plasmids in this important human and animal pathogen. • The pCP13 conjugation locus represents an evolutionarily conserved transfer region within the clostridial genus. • This study has implications for the dissemination of toxins including the clinically relevant BEC enterotoxin. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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38. The FxRxHrS Motif: A Conserved Region Essential for DNA Binding of the VirR Response Regulator from Clostridium perfringens
- Author
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McGowan, Sheena, Lucet, Isabelle S., Cheung, Jackie K., Awad, Milena M., Whisstock, James C., and Rood, Julian I.
- Subjects
- *
GENETIC transduction , *CLOSTRIDIUM perfringens , *GENETIC transcription - Abstract
The VirSR two-component signal transduction pathway regulates virulence and toxin production in Clostridium perfringens, the causative agent of gas gangrene. The response regulator, VirR, binds to repeat sequences located upstream of the promoter and is directly responsible for the transcriptional activation of pfoA, the structural gene for the cholesterol-dependent cytolysin, perfringolysin O. Comparative sequence analysis of the 236 amino acid residue VirR protein revealed a two-domain structure: a typical N-terminal response regulator domain and an uncharacterised C-terminal domain. Database searching revealed that over 40 other proteins, many of which appeared to be response regulators or transcriptional activators, had homology with the VirR C-terminal domain (VirRc). Multiple sequence alignment of this VirRc family revealed a highly conserved region that was designated the FxRxHrS motif. By deletion analysis this motif was shown to be essential for the functional integrity of the VirR protein. Alanine scanning mutagenesis and subsequent phenotypic analysis indicated that conserved residues located within the motif were required for activity. These residues extended from L179 to N194. More detailed site-directed mutagenesis showed that amino acid residues R186, H188 and S190 were essential for activity since even conservative substitutions in these positions resulted in non-functional proteins. Three of the mutant proteins, R186K, S190A and S190C, were purified and shown by in vitro gel shift analysis to be unable to bind to the specific target DNA with the same efficiency as the wild-type protein. These data reveal for the first time that VirRc functions as a DNA binding domain in which the highly conserved FxRxHrS motif has a functional role. These studies have important implications for this new family of transcriptional factors since they imply that the conserved FxRxHrS motif may be involved in DNA binding in all of these proteins, irrespective of their biological role. [Copyright &y& Elsevier]
- Published
- 2002
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39. Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.
- Author
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Mohamed, Ahmed, Chan, Helena, Luhur, Johana, Bauda, Elda, Gallet, Benoit, Morlot, Cécile, Cole, Louise, Awad, Milena, Crawford, Simon, Lyras, Dena, Rudner, David Z., and Rodrigues, Christopher D.A.
- Subjects
- *
CHROMOSOME segregation , *BACTERIAL spores , *PEPTIDOGLYCANS , *STEM cells , *SYNTHETIC enzymes , *HYDROLASES - Abstract
Asymmetric division, a hallmark of endospore development, generates two cells, a larger mother cell and a smaller forespore. Approximately 75% of the forespore chromosome must be translocated across the division septum into the forespore by the DNA translocase SpoIIIE. Asymmetric division also triggers cell-specific transcription, which initiates septal peptidoglycan remodeling involving synthetic and hydrolytic enzymes. How these processes are coordinated has remained a mystery. Using Bacillus subtilis , we identified factors that revealed the link between chromosome translocation and peptidoglycan remodeling. In cells lacking these factors, the asymmetric septum retracts, resulting in forespore cytoplasmic leakage and loss of DNA translocation. Importantly, these phenotypes depend on septal peptidoglycan hydrolysis. Our data support a model in which SpoIIIE is anchored at the edge of a septal pore, stabilized by newly synthesized peptidoglycan and protein-protein interactions across the septum. Together, these factors ensure coordination between chromosome translocation and septal peptidoglycan remodeling to maintain spore development. • Coordination of cell wall remodeling and chromosome segregation at a septal pore • Balance between cell wall hydrolysis and synthesis is required for pore stability • Pore stability is reinforced by the highly conserved SpoIIIAH-SpoIIQ interaction • Coordinating chromosome segregation and cytokinesis is fundamental for development Coordinating chromosome segregation with cytokinesis is of primordial importance during development. In this issue, Mohamed et al. define how coordination between chromosome segregation and cell wall remodeling contributes to critical aspects of genetic and transcriptional compartmentalization during endospore formation in bacteria, one of the earliest forms of cellular development on Earth. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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40. Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens.
- Author
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Myers, Garry S. A., Rasko, David A., Cheung, Jackie K., Ravel, Jacques, Seshadri, Rekha, DeBoy, Robert T., Qinghu Ren, Varga, John, Awad, Milena M., Brinkac, Lauren M., Daugherty, Sean C., Haft, Daniel H., Dodson, Robert J., Madupu, Ramana, Nelson, William C., Rosovitz, M. J., Sullivan, Steven A., Khouri, Hoda, Dimitrov, George I., and Watkins, Kisha L.
- Subjects
- *
CLOSTRIDIUM perfringens , *GENOMES , *GRAM-positive bacteria , *GENES , *PATHOGENIC microorganisms - Abstract
Clostridium perfringens is a Gram-positive, anaerobic spore-forming bacterium commonly found in soil, sediments, and the human gastrointestinal tract. C. perfringens is responsible for a wide spectrum of disease, including food poisoning, gas gangrene (clostridial myonecrosis), enteritis necroticans, and non-foodborne gastrointestinal infections. The complete genome sequences of Clostridium perfringens strain ATCC 13124, a gas gangrene isolate and the species type strain, and the enterotoxin-producing food poisoning strain SM101, were determined and compared with the published C. perfringens strain 13 genome. Comparison of the three genomes revealed considerable genomic diversity with >300 unique "genomic islands" identified, with the majority of these islands unusually clustered on one replichore. PCR-based analysis indicageted that the large genomic islands are widely variable across a large collection of C. perfringens strains. These islands encode genes that correlate to differences in virulence and phenotypic characteristics of these strains. Significant differences between the strains include numerous novel mobile elements and genes encoding metabolic capabilities, strain-specific extracellular polysaccharide capsule, sporulation factors, toxins, and other secreted enzymes, providing substantial insight into this medically important bacterial pathogen. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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41. Lectin Activity of the TcdA and TcdB Toxins of Clostridium difficile.
- Author
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Hartley-Tassell LE, Awad MM, Seib KL, Scarselli M, Savino S, Tiralongo J, Lyras D, Day CJ, and Jennings MP
- Subjects
- Animals, Cell Survival, Chlorocebus aethiops, Cloning, Molecular, Polysaccharides, Vero Cells, Bacterial Proteins metabolism, Bacterial Toxins metabolism, Clostridioides difficile metabolism, Enterotoxins metabolism, Lectins metabolism
- Abstract
Clostridium difficile is a major cause of hospital-acquired antibiotic-associated diarrhea. C. difficile produces two cytotoxins, TcdA and TcdB; both toxins are multidomain proteins that lead to cytotoxicity through the modification and inactivation of small GTPases of the Rho/Rac family. Previous studies have indicated that host glycans are targets for TcdA and TcdB, with interactions thought to be with both α- and β-linked galactose. In the current study, screening of glycan arrays with different domains of TcdA and TcdB revealed that the binding regions of both toxins interact with a wider range of host glycoconjugates than just terminal α- and β-linked galactose, including blood groups, Lewis antigens, N -acetylglucosamine, mannose, and glycosaminoglycans. The interactions of TcdA and TcdB with ABO blood group and Lewis antigens were assessed by surface plasmon resonance (SPR). The blood group A antigen was the highest-affinity ligand for both toxins. Free glycans alone or in combination were unable to abolish Vero cell cytotoxicity by TcdB. SPR competition assays indicate that there is more than one glycan binding site on TcdB. Host glycoconjugates are common targets of bacterial toxins, but typically this binding is to a specific structure or related structures. The binding of TcdA and TcdB is to a wide range of host glycans providing a wide range of target cells and tissues in vivo ., (Copyright © 2019 Hartley-Tassell et al.)
- Published
- 2019
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42. Clostridium sordellii Pathogenicity Locus Plasmid pCS1-1 Encodes a Novel Clostridial Conjugation Locus.
- Author
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Vidor CJ, Watts TD, Adams V, Bulach D, Couchman E, Rood JI, Fairweather NF, Awad M, and Lyras D
- Subjects
- Computational Biology, Genes, Bacterial, Multigene Family, Clostridium sordellii genetics, Conjugation, Genetic, Gene Transfer, Horizontal, Genetic Loci, Plasmids
- Abstract
A major virulence factor in Clostridium sordellii -mediated infection is the toxin TcsL, which is encoded within a region of the genome called the pathogenicity locus (PaLoc). C. sordellii isolates carry the PaLoc on the pCS1 family of plasmids, of which there are four characterized members. Here, we determined the potential mobility of pCS1 plasmids and characterized a fifth unique pCS1 member. Using a derivative of the pCS1-1 plasmid from strain ATCC 9714 which had been marked with the ermB erythromycin resistance gene, conjugative transfer into a recipient C. sordellii isolate, R28058, was demonstrated. Bioinformatic analysis of pCS1-1 identified a novel conjugation gene cluster defined as the C. sordellii transfer ( cst ) locus. Interruption of genes within the cst locus resulted in loss of pCS1-1 transfer, which was restored upon complementation in trans These studies provided clear evidence that genes within the cst locus are essential for the conjugative transfer of pCS1-1. The cst locus is present on all pCS1 subtypes, and homologous loci were identified on toxin-encoding plasmids from Clostridium perfringens and Clostridium botulinum and also carried within genomes of Clostridium difficile isolates, indicating that it is a widespread clostridial conjugation locus. The results of this study have broad implications for the dissemination of toxin genes and, potentially, antibiotic resistance genes among members of a diverse range of clostridial pathogens, providing these microorganisms with a survival advantage within the infected host. IMPORTANCE C. sordellii is a bacterial pathogen that causes severe infections in humans and animals, with high mortality rates. While the pathogenesis of C. sordellii infections is not well understood, it is known that the toxin TcsL is an important virulence factor. Here, we have shown the ability of a plasmid carrying the tcsL gene to undergo conjugative transfer between distantly related strains of C. sordellii , which has far-reaching implications for the ability of C. sordellii to acquire the capacity to cause disease. Plasmids that carry tcsL encode a previously uncharacterized conjugation locus, and individual genes within this locus were shown to be required for conjugative transfer. Furthermore, homologues on toxin plasmids from other clostridial species were identified, indicating that this region represents a novel clostridial conjugation locus. The results of this study have broad implications for the dissemination of virulence genes among members of a diverse range of clostridial pathogens., (Copyright © 2018 Vidor et al.)
- Published
- 2018
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43. Utility of the clostridial site-specific recombinase TnpX to clone toxic-product-encoding genes and selectively remove genomic DNA fragments.
- Author
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Adams V, Bantwal R, Stevenson L, Cheung JK, Awad MM, Nicholson J, Carter GP, Mackin KE, Rood JI, and Lyras D
- Subjects
- Bacterial Proteins genetics, Clostridium perfringens enzymology, DNA Nucleotidyltransferases, Escherichia coli metabolism, Genetic Complementation Test, Recombinases genetics, Recombination, Genetic, Bacterial Proteins metabolism, Cloning, Molecular methods, Clostridium perfringens genetics, DNA, Bacterial genetics, Escherichia coli genetics, Genome, Bacterial, Recombinases metabolism
- Abstract
TnpX is a site-specific recombinase responsible for the excision and insertion of the transposons Tn4451 and Tn4453 in Clostridium perfringens and Clostridium difficile, respectively. Here, we exploit phenotypic features of TnpX to facilitate genetic mutagenesis and complementation studies. Genetic manipulation of bacteria often relies on the use of antibiotic resistance genes; however, a limited number are available for use in the clostridia. The ability of TnpX to recognize and excise specific DNA fragments was exploited here as the basis of an antibiotic resistance marker recycling system, specifically to remove antibiotic resistance genes from plasmids in Escherichia coli and from marked chromosomal C. perfringens mutants. This methodology enabled the construction of a C. perfringens plc virR double mutant by allowing the removal and subsequent reuse of the same resistance gene to construct a second mutation. Genetic complementation can be challenging when the gene of interest encodes a product toxic to E. coli. We show that TnpX represses expression from its own promoter, PattCI, which can be exploited to facilitate the cloning of recalcitrant genes in E. coli for subsequent expression in the heterologous host C. perfringens. Importantly, this technology expands the repertoire of tools available for the genetic manipulation of the clostridia.
- Published
- 2014
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44. TcsL is an essential virulence factor in Clostridium sordellii ATCC 9714.
- Author
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Carter GP, Awad MM, Hao Y, Thelen T, Bergin IL, Howarth PM, Seemann T, Rood JI, Aronoff DM, and Lyras D
- Subjects
- Animals, Blotting, Southern, Blotting, Western, Chlorocebus aethiops, Clostridium sordellii pathogenicity, Genes, Bacterial genetics, Mice, Mice, Inbred BALB C, Polymerase Chain Reaction, Vero Cells, Virulence, Bacterial Toxins genetics, Clostridium sordellii genetics, Virulence Factors genetics
- Abstract
Clostridium sordellii is an important pathogen of humans and animals, causing a range of diseases, including myonecrosis, sepsis, and shock. Although relatively rare in humans, the incidence of disease is increasing, and it is associated with high mortality rates, approaching 70%. Currently, very little is known about the pathogenesis of C. sordellii infections or disease. Previous work suggested that the lethal large clostridial glucosylating toxin TcsL is the major virulence factor, but a lack of genetic tools has hindered our ability to conclusively assign a role for TcsL or, indeed, any of the other putative virulence factors produced by this organism. In this study, we have developed methods for the introduction of plasmids into C. sordellii using RP4-mediated conjugation from Escherichia coli and have successfully used these techniques to insertionally inactivate the tcsL gene in the reference strain ATCC 9714, using targetron technology. Virulence testing revealed that the production of TcsL is essential for the development of lethal infections by C. sordellii ATCC 9714 and also contributes significantly to edema seen during uterine infection. This study represents the first definitive identification of a virulence factor in C. sordellii and opens the way for in-depth studies of this important human pathogen at the molecular level.
- Published
- 2011
- Full Text
- View/download PDF
45. Novel use of tryptose sulfite cycloserine egg yolk agar for isolation of Clostridium perfringens during an outbreak of necrotizing enterocolitis in a neonatal unit.
- Author
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Kotsanas D, Carson JA, Awad MM, Lyras D, Rood JI, Jenkin GA, Stuart RL, and Korman TM
- Subjects
- Agar, Clostridium Infections epidemiology, Clostridium Infections microbiology, Cycloserine metabolism, Egg Yolk metabolism, Humans, Infant, Newborn, Organic Chemicals metabolism, Sensitivity and Specificity, Sulfites metabolism, Bacteriological Techniques methods, Clostridium Infections diagnosis, Clostridium perfringens isolation & purification, Culture Media chemistry, Disease Outbreaks, Enterocolitis, Necrotizing epidemiology, Enterocolitis, Necrotizing microbiology
- Abstract
Clostridium perfringens has been associated with necrotizing enterocolitis (NEC), which is a serious disease of neonates. Our study describes the novel use of selective tryptose sulfite cycloserine with egg yolk agar (TSC-EYA) during a nursery outbreak. This medium provides a rapid, sensitive, and accurate presumptive identification of C. perfringens.
- Published
- 2010
- Full Text
- View/download PDF
46. The NanI and NanJ sialidases of Clostridium perfringens are not essential for virulence.
- Author
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Chiarezza M, Lyras D, Pidot SJ, Flores-Díaz M, Awad MM, Kennedy CL, Cordner LM, Phumoonna T, Poon R, Hughes ML, Emmins JJ, Alape-Girón A, and Rood JI
- Subjects
- Animals, Bacterial Proteins genetics, Cell Line, Tumor, Cell Survival, Gene Knockout Techniques, Mice, Mice, Inbred BALB C, Mutagenesis, Insertional, Neuraminidase genetics, Survival Analysis, Virulence, Virulence Factors genetics, Bacterial Proteins physiology, Clostridium perfringens enzymology, Clostridium perfringens pathogenicity, Gas Gangrene microbiology, Neuraminidase physiology, Virulence Factors physiology
- Abstract
The essential toxin in Clostridium perfringens-mediated gas gangrene or clostridial myonecrosis is alpha-toxin, although other toxins and extracellular enzymes may also be involved. In many bacterial pathogens extracellular sialidases are important virulence factors, and it has been suggested that sialidases may play a role in gas gangrene. C. perfringens strains have combinations of three different sialidase genes, two of which, nanI and nanJ, encode secreted sialidases. The nanI and nanJ genes were insertionally inactivated by homologous recombination in derivatives of sequenced strain 13 and were shown to encode two functional secreted sialidases, NanI and NanJ. Analysis of these derivatives showed that NanI was the major sialidase in this organism. Mutation of nanI resulted in loss of most of the secreted sialidase activity, and the residual activity was eliminated by subsequent mutation of the nanJ gene. Only a slight reduction in the total sialidase activity was observed in a nanJ mutant. Cytotoxicity assays using the B16 melanoma cell line showed that supernatants containing NanI or overexpressing NanJ enhanced alpha-toxin-mediated cytotoxicity. Finally, the ability of nanI, nanJ, and nanIJ mutants to cause disease was assessed in a mouse myonecrosis model. No attenuation of virulence was observed for any of these strains, providing evidence that neither the NanI sialidase nor the NanJ sialidase is essential for virulence.
- Published
- 2009
- Full Text
- View/download PDF
47. Alpha-toxin of Clostridium perfringens is not an essential virulence factor in necrotic enteritis in chickens.
- Author
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Keyburn AL, Sheedy SA, Ford ME, Williamson MM, Awad MM, Rood JI, and Moore RJ
- Subjects
- Animals, Bacterial Toxins, Clostridium perfringens isolation & purification, Clostridium perfringens pathogenicity, Enteritis pathology, Humans, Necrosis, Virulence, Calcium-Binding Proteins physiology, Chickens microbiology, Clostridium perfringens physiology, Enteritis metabolism, Enteritis microbiology, Type C Phospholipases physiology, Virulence Factors physiology
- Abstract
The Clostridium perfringens alpha-toxin has previously been implicated as the major virulence factor in necrotic enteritis in chickens, although definitive proof has not been reported. In this study an alpha-toxin mutant was constructed in a virulent chicken isolate and shown to retain full virulence in a chicken disease model. These results demonstrated that alpha-toxin is not an essential virulence factor in the pathogenesis of necrotic enteritis in chickens.
- Published
- 2006
- Full Text
- View/download PDF
48. The alpha-toxin of Clostridium septicum is essential for virulence.
- Author
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Kennedy CL, Krejany EO, Young LF, O'Connor JR, Awad MM, Boyd RL, Emmins JJ, Lyras D, and Rood JI
- Subjects
- Alleles, Animals, Bacterial Toxins genetics, Clostridium genetics, Clostridium metabolism, Clostridium Infections microbiology, Clostridium Infections pathology, Conjugation, Genetic, DNA, Bacterial genetics, Disease Models, Animal, Escherichia coli genetics, Leukostasis microbiology, Mice, Muscle, Skeletal pathology, Necrosis microbiology, Plasmids, Virulence, Virulence Factors genetics, Bacterial Toxins metabolism, Clostridium pathogenicity, Muscle, Skeletal microbiology, Virulence Factors metabolism
- Abstract
Clostridium septicum is the causative agent of spontaneous gas gangrene or atraumatic myonecrosis, a sudden and frequently fatal infection that is increasingly associated with malignancy of the colon. Little is known about the disease process although the focus of virulence studies has been the alpha-toxin, a pore-forming cytolysin that is encoded by the csa gene and secreted as an inactive protoxin. Until now a lack of techniques for the genetic manipulation of C. septicum has hindered the use of molecular approaches to understand pathogenesis. By introducing plasmids by conjugation from Escherichia coli, we have developed methods for the genetic manipulation of C. septicum and constructed a chromosomal csa mutant by allelic exchange. Virulence testing of an isogenic series of strains consisting of the wild type, the csa mutant, and a csa mutant complemented with the wild-type csa gene revealed that the development of fulminant myonecrosis in mice was dependent on the ability to produce a functional haemolytic alpha-toxin. Furthermore, the inhibition of leukocyte influx into the lesion, which is very typical of clostridial myonecrosis, was also dependent on the ability to produce alpha-toxin. This study represents the first definitive identification of a virulence factor in this organism and opens the way for further studies that will delineate the role of other putative virulence factors in this significant pathogen.
- Published
- 2005
- Full Text
- View/download PDF
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