128 results on '"Arduini F"'
Search Results
2. S28-02 Miniaturized electrochemical (bio)sensors for the detection of warfare agents.
- Author
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Arduini, F.
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MILITARY science , *DETECTORS , *ELECTROCHEMICAL sensors - Published
- 2023
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- View/download PDF
3. Origami multiple paper-based electrochemical biosensors for pesticide detection.
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Arduini, F., Cinti, S., Caratelli, V., Amendola, L., Palleschi, G., and Moscone, D.
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ELECTROCHEMICAL sensors , *ORIGAMI , *PESTICIDE analysis , *BIOSENSORS , *SUBSTRATES (Materials science) , *ORGANOPHOSPHORUS insecticides - Abstract
Abstract Herein, we propose the first three-dimensional origami paper-based device for the detection of several classes of pesticides by combining different enzyme-inhibition biosensors. This device was developed by integrating two different office paper-based screen-printed electrodes and multiple filter paper-based pads to load enzymes and enzymatic substrates. The versatile analysis of different pesticides was carried by folding and unfolding the filter paper-based structure, without any addition of reagents and any sample treatment (i.e. dilution, filtration, pH adjustment). The paper-based platform was employed to detect paraoxon, 2,4-dichlorophenoxyacetic acid, and atrazine by exploiting the capability of these different types of pesticides (i.e. organophosphorus insecticides, phenoxy-acid herbicides, and triazine herbicide) to inhibit butyrylcholinesterase, alkaline phosphatase, and tyrosinase, respectively. The degree of inhibition correlating to the quantity of pesticides was evaluated by chronoamperometrically monitoring the enzymatic activity in the absence and in the presence of pesticides by using a portable potentiostat. To improve the sensitivity, the paper-based electrodes were modified with carbon black alone in the case of platforms for 2,4-dichlorophenoxyacetic acid and atrazine detection, or decorated with Prussian blue nanoparticles for the detection of paraoxon. The paper-based device was applied for the detection of paraoxon, 2,4-dichlorophenoxyacetic acid, and atrazine at ppb level in both standard solutions and river water sample. The accuracy of this origami multiple paper-based electrochemical biosensor was evaluated in river water by recovery studies, obtaining satisfactory values (e.g. for paraoxon 90 ± 1% and 88 ± 2%, for 10 and 20 ppb, respectively). The proposed three-dimensional origami paper device allows for rapid, cost-effective and accurate pesticide detection in surface water as a result of combining filter and office papers, screen-printing, wax-printing and nanomaterial technology. Graphical abstract fx1 Highlights • 3D paper-based origami device for different types of pesticides. • Reagentless measurement of pesticides. • Inhibition-based enzymatic biosensors. • Wax/screen-printing, paper and nanomaterials for a laboratory-free platform. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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4. Recent advances in biosensors based on enzyme inhibition.
- Author
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Amine, A., Arduini, F., Moscone, D., and Palleschi, G.
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BIOSENSORS , *ENZYME inhibitors , *ENVIRONMENTAL health , *DRUG analysis , *MEDICAL screening - Abstract
Enzyme inhibitors like drugs and pollutants are closely correlated to human and environmental health, thus their monitoring is of paramount importance in analytical chemistry. Enzymatic biosensors represent cost-effective, miniaturized and easy to use devices; particularly biosensors based on enzyme inhibition are useful analytical tools for fast screening and monitoring of inhibitors. The present review will highlight the research carried out in the last 9 years (2006–2014) on biosensors based on enzyme inhibition. We underpin the recent advances focused on the investigation in new theoretical approachs and in the evaluation of biosensor performances for reversible and irreversible inhibitors. The use of nanomaterials and microfluidic systems as well as the applications of the various biosensors in real samples is critically reviewed, demonstrating that such biosensors allow the development of useful devices for a fast and reliable alarm system. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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5. Effective electrochemical sensor based on screen-printed electrodes modified with a carbon black-Au nanoparticles composite.
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Arduini, F., Zanardi, C., Cinti, S., Terzi, F., Moscone, D., Palleschi, G., and Seeber, R.
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ELECTROCHEMICAL sensors , *ELECTRODES , *CARBON-black , *GOLD nanoparticles , *COMPOSITE materials - Abstract
A screen-printed electrode (SPE) modified with a carbon black (CB)-Au nanoparticles (AuNPs) composite is assembled and tested. Electrochemical and morphological investigations highlight the physico-chemical properties of the resulting AuNP-CB-SPE amperometric device with respect to SPEs modified with a single component of the nanocomposite. The effective performance of such a modified electrode in activating electrocatalytic processes, consisting both in oxidation and reduction reactions, is demonstrated. In particular, electrochemical tests on analytes such as glucose, hydrogen peroxide, hydroquinone, and ascorbic acid, evidence that the composite possesses electrocatalytic performance well superior with respect to the relevant mono-component modified SPE. As a consequence, a meaningful lowering of the peak potentials and improvement of the sensor sensitivities is observed when using AuNP-CB-SPEs with respect to both CB-SPEs and AuNP-SPEs. In the case of H 2 O 2 reduction, the occurrence of the electrochemical process at less negative potentials is coupled to an improvement of sensor sensitivity of about one order of magnitude. Concurrently, lower limit of detections, ranging from 20 to 99% less, have been obtained for the major part of the analytes studied, i.e. glucose, hydrogen peroxide and hydroquinone. Preliminary results reported here indicate that AuNP-CB-SPE can be proposed as an efficient amperometric sensor to be used in many analytical applications. [ABSTRACT FROM AUTHOR]
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- 2015
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6. Biosensing technology for sustainable food safety.
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Scognamiglio, V., Arduini, F., Palleschi, G., and Rea, G.
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FOOD safety , *BIOSENSORS , *NANOTECHNOLOGY , *FOOD quality , *EMBEDDED computer systems - Abstract
Food and diet are closely linked to human health, and new emerging research fields are attempting to guarantee improvements in food quality and safety. Biosensor technology represents a cutting-edge frontier in environmental and biomedical diagnosis and is at the forefront in the agrifood sector. Smart monitoring of nutrients and fast screening of biological and chemical contaminants are some of the key evolving issues challenging the assessment of food quality and safety. Advances in materials science and nanotechnology, electromechanical and microfluidic systems, protein engineering and biomimetics design are boosting sensing technology from bench to market. This review highlights current and future trends in analytical diagnostic tools focused on the food industry and target analytes to support healthier nutrition. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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7. May Etanercept and PTH (1-34) association heal erosions in early rheumatoid arthritis? A pilot study.
- Author
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Migliore, A., Massafra, U., Bizzi, E., Argento, G., Diamanti, A. Picchianti, Germano, V., Tormenta, S., Arduini, F., Iannessi, F., Granata, M., and Laganà, B.
- Abstract
Introduction: Rheumatoid arthritis (RA) is characterized by the formation in the joints of an inflammatory tissue, which causes the appearance of localized erosions on the margins of the joints. The molecular mechanism that causes the bone erosion is multifactorial. Inflammatory cytokines imbalance and OPG-RANK-L system are involved. Objective of the Study: The aim of the study is to evaluate the possibility of inducing healing or reduction in the number of erosions in Rheumatoid Arthritis patients treated with anti- TNF-alpha adding Teriparatide (PTH1-34) to standard treatment with anti-TNF. Patients and Methods: Twenty adult patients with active RA diagnosed according to American Rheumatism Association (ARA) criteria at least 6 months before study begin were enrolled. Only patients affected by established RA (6 to 18 months from symptoms beginning) were recruited. Eligible patients were randomized to receive a standard dosage of etanercept (50 mg/week) or etanercept at same dosage with an addition of teriparatide (20 mg). Evaluation of eventual healing of arthritic erosions by magnetic resonance imaging was performed at time zero and then at twelve months. The following evaluation was assessed at baseline and after 12 months according to the Outcome Measures in Rheumatology Clinical Trials (OMERACT) definitions: number of erosion and presence or absence of synovitis, effusion and bone oedema. A comparative examination of quantitative and qualitative assessment of each parameter was applied. Plain radiographs of the hands were obtained at baseline and 52 weeks. Radiographs were scored blindly using the van der Heijde modification of the Sharp method. Safety of each treatment was evaluated by means of the adverse events (AES) evaluation and report. Results: There were no significant differences in baseline characteristics between the groups. The study did not achieve its primary endpoint of healing erosions. In the active arm no healing of erosions was found. At 52 weeks, there were no new MRI erosions in two arms. Bone oedema scores were significantly improved at 52 weeks in favour of both treatments versus baseline scores, without inter-groups differences. X-ray patterns were unchanged in all patients of both groups. No new erosions or previous erosions' healing were observed. No AEs were reported. Patients from both groups demonstrated a significant reduction in the DAS 28 scores at 52 weeks (p < 0.005) if compared with baseline values. Conclusions: These data confirm rapid control of inflammation and MRI damage benefits after Etanercept administration without a significant improvement in MRI findings after concomitant addition of teriparatide. Even though these results could seem to suggest to avoid the simultaneous use of these two drugs to treat RA erosions, further studies might be suggested to asses if sequential administration of an anabolic agent such as Teriparatide, after achieving clinical remission, may be able to improve bone damage. [ABSTRACT FROM AUTHOR]
- Published
- 2012
8. Construction, assembling and application of a trehalase–GOD enzyme electrode system
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Antonelli, M.L., Arduini, F., Laganà, A., Moscone, D., and Siliprandi, V.
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BIOSENSORS , *GLYCOSIDASES , *ENZYME electrodes , *OXIDASES , *GLUCOSE , *HYDROLYSIS - Abstract
Abstract: Trehalose is a disaccharide important in foods, serving as a glucose source in many and also as an additive in the food preparation. Because of its peculiar physico-chemical properties it plays an important role as preservative in drying and deep-freezing treatments. A new biosensor for trehalose determination has been realized by means of a flow system, based on a reactor in which the trehalase enzyme catalyses its hydrolysis into two α,d-glucose molecules, and a GOD (glucose oxidase) amperometric biosensor is employed for the glucose determination. The optimum operative conditions have been laid out and a particular attention has been paid to the immobilization procedure of the two enzymes. The electrode used is of the SPE (screen-printed electrode) type and has been activated with the Prussian Blue (PB) and then assembled using GOD immobilized with Nafion. The reactor has been prepared with the trehalase enzyme chemically immobilized on an Immunodyne ABC membrane. As demonstration of its utility, the biosensor has been tested on a real sample of Boletus edulis mushroom. [Copyright &y& Elsevier]
- Published
- 2009
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9. 210 An analysis of CDH1 -160 C/A promoter polymorphism and the risk of diffuse gastric cancer in Italy.
- Author
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Ruzzo, A., Spino, C., Graziano, F., Testa, E., Costagliola, A., Arduini, F., Santini, D., Andreoni, F., and Magnani, M.
- Published
- 2003
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10. 201 Analysis of G/A SNP change at position 2494 in the E-cadherin gene in Italian patients with sporadic, diffuse gastric cancer.
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Ruzzo, A., Graziano, F., Costagliola, A., Testa, E., Andreoni, F., Arduini, F., Antolini, A., Bearzi, I., De Gaetano, A., and Magnari, M.
- Published
- 2003
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11. Laccase biosensor based on screen-printed electrode modified with thionine–carbon black nanocomposite, for Bisphenol A detection.
- Author
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Portaccio, M., Di Tuoro, D., Arduini, F., Moscone, D., Cammarota, M., Mita, D.G., and Lepore, M.
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LACCASE , *BIOSENSORS , *ELECTRODES , *THIONINE , *CARBON-black , *NANOCOMPOSITE materials , *BISPHENOL A - Abstract
Highlights: [•] A novel laccase-modified screen printed electrode (SPE) has been developed. [•] Bionanocomposite sensor is optimized in terms of enzyme loading, pH and applied potential. [•] Linear range, sensitivity and LOD are assessed for BPA determination. [•] The biosensor has been challenged in tomato juice samples. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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12. A thionine-modified carbon paste amperometric biosensor for catechol and bisphenol A determination
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Portaccio, M., Di Tuoro, D., Arduini, F., Lepore, M., Mita, D.G., Diano, N., Mita, L., and Moscone, D.
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CARBON , *CONDUCTOMETRIC analysis , *BIOSENSORS , *CATECHOL , *BISPHENOL A , *ELECTRODES , *ELECTROCHEMICAL analysis - Abstract
Abstract: A thionine-modified carbon paste electrode for catechol and Bisphenol A (BPA) detection is presented. Graphite powder was modified by adsorbing thionine as electrochemical mediator. The electrochemical response of the modified carbon paste electrode (CPE) was determined before electrode modification with tyrosinase. Then, tyrosinase was added in order to assemble a biosensor. Once established the best operative conditions, an interelectrode reproducibility around 7% was obtained and the resulting biosensor showed improved sensitivities and (S =139.6±1.1nA/μM for catechol and S =85.4±1.5nA/μM for BPA) in comparison with the biosensor constructed without thionine (S =104.4±0.5nA/μM for catechol and S =51.1±0.6nA/μM for BPA) and low detection limits (0.15μM for both the electrodes and analytes). Also the comparison with the results reported in the literature showed higher sensitivity and lower detection limit for our biosensor. Moreover the functioning of the thionine-tyrosinase CPE was validated following a biodegradation process of water polluted by BPA and comparing the time changes of BPA concentration inferred by the biosensor calibration curve and those determined by means of HPLC measurements. [Copyright &y& Elsevier]
- Published
- 2010
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13. Enzymatic determination of BPA by means of tyrosinase immobilized on different carbon carriers
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Mita, D.G., Attanasio, A., Arduini, F., Diano, N., Grano, V., Bencivenga, U., Rossi, S., Amine, A., and Moscone, D.
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NANOTUBES , *FULLERENES , *BIOSENSORS , *HYDROCARBONS - Abstract
Abstract: Different tyrosinase carbon paste modified electrodes to determine bisphenol A (BPA) concentration in aqueous solutions have been constructed. Variables examined were in the carbon paste composition and in particular: (i) the immobilized enzyme amount; (ii) the carbon type (powder, single or multi-walled nanotubes); (iii) the nature of the pasting oil (mineral oil, hexadecane and dodecane). For each biosensor type the amperometric response was evaluated with reference to the linear range and sensitivity. Constant reference has been made to the amperometric signals obtained, under the same experimental conditions, towards the catechol, a specific phenolic substrate for tyrosinase. The most efficient biosensors were those constructed by using the following composition for the carbon paste: 10% of tyrosinase, 45% of single wall carbon nanotubes (SWCN) and 45% of mineral oil. This biosensor formulation displayed the following electrochemical characteristics: a sensitivity equal to 138μA/mM, LOD of 0.02μM (based on three times the S/N ratio), linear range of 0.1–12μM and response time of 6min. This experimental work represents a first attempt at construction of a new carbon nanotube-tyrosinase based biosensor able to determine the concentration of BPA, one of the most ubiquitous and hazardous endocrine disruptors which can pollute the drinking and surface water, as well as many products of the food chain. [Copyright &y& Elsevier]
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- 2007
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14. Novel carbon black-cobalt phthalocyanine nanocomposite as sensing platform to detect organophosphorus pollutants at screen-printed electrode.
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Cinti, S., Neagu, D., Carbone, M., Cacciotti, I., Moscone, D., and Arduini, F.
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CARBON-black , *POLLUTANT identification , *PHTHALOCYANINES , *NANOCOMPOSITE materials , *ORGANOPHOSPHORUS compounds , *ELECTRODES - Abstract
A facile “one-step” route to produce a homogenous and highly stable cobalt phthalocyanine (CoPc)-based dispersion by using carbon black (CB) as supporting material is reported. Herein, CB is proposed as effective material to load CoPc in order to obtain a CB/CoPc hybrid nanocomposite dispersion suitable for modifying screen-printed electrodes (SPEs) by an easy and automatable drop casting approach. CoPc resulted anchored to CB by a non-covalent physisorption, confirmed by IR and UV-visible spectroscopies, allowing to preserve the electrochemical performances of CoPc. The resulting CB/CoPc-modified SPE was tested as sensing tool to detect thiocholine, an enzymatic product of butyrylcholinesterase (BChE). The use of CB/CoPc leads to a highly sensitive thiocholine detection by applying a low potential (+0.05 V vs. internal reference) without fouling problem, a typical drawback that affects the thiol electrochemical detection. The favorable characteristics of the sensor were exploited for an easy BChE biosensor fabrication that renders this biosensor well suitable for mass-production. This electrochemical monoenzymatic biosensor was then challenged towards paraoxon, chosen as model organophosphorous pesticide, obtaining a low detection limit (18 nM). The suitability of the biosensor was tested in a waste water sample obtaining satisfactory recovery values, thus demonstrating its capability in such complex matrix. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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15. Cadmium determination in natural water samples with an automatic multisyringe flow injection system coupled to a flow-through screen printed electrode
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Henríquez, C., Laglera, L.M., Alpizar, M.J., Calvo, J., Arduini, F., and Cerdà, V.
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FLOW injection analysis , *WATER sampling , *CADMIUM , *SCREEN process printing , *ELECTRODES , *HEAVY metals , *ACUTE toxicity testing - Abstract
Abstract: Heavy metals, as cadmium, attract a rising attention in environmental studies due to their increasing release by human activities and acute toxicity. In situ analytical methods are needed to minimize current uncertainties caused by the transport and conservation of samples. Here, we present the completely automatic determination of Cd in natural waters using a newly developed screen printed electrode sensor (SPE), inserted in a homemade purpose-built flow cell coupled to a Multi-Syringe Flow Injection Analysis system (MSFIA). The working electrode of SPEs was constituted by a carbon film modified with Nafion. Cd was plated on an in situ bismuth film and determined using Square Wave Anodic Stripping Voltammetry. Different chemical conditions of deposition and stripping were studied. A sample/acetic buffer mixture was found to be a well suited medium to form the Bi film and perform the analysis. Cd was quantified via calibration by on line standard additions. The limit of detection was found to be 0.79μgL−1, well below the limit stipulated by the European directive (5μgL−1). Good sample throughput (14h−1) and low consumption of reagent and sample (1.3mL) were also obtained in line with previous works in Cd flow analysis. [Copyright &y& Elsevier]
- Published
- 2012
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16. An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos
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Di Tuoro, D., Portaccio, M., Lepore, M., Arduini, F., Moscone, D., Bencivenga, U., and Mita, D.G.
- Subjects
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BIOSENSORS , *ACETYLCHOLINESTERASE , *DICHLORVOS , *PESTICIDES , *ACETIC acid , *ENZYME inhibitors - Abstract
The characterization of an economic and ease-to-use carbon paste acetylcholinesterase (AChE) based biosensor to determine the concentration of pesticides Paraoxon and Dichlorvos is discussed. AChE hydrolyses acetylthiocholine (ATCh) in thiocoline (TC) and acetic acid (AA). When AChE is immobilized into a paste carbon working electrode kept at +410mV vs. Ag/AgCl electrode, the enzyme reaction rate using acetylthiocholine chloride (ATCl) as substrate is monitored as a current intensity. Because Paraoxon and Dichlorvos inhibit the AChE reaction, the decrease of the current intensity, at fixed ATCl concentration, is a measure of their concentration. Linear calibration curves for Paraoxon and Dichlorvos determination have been obtained. The detection limits resulted to be 0.86ppb and 4.2ppb for Paraoxon and Dichlorvos, respectively, while the extension of the linear range was up 23ppb for the former pesticide and up to 33ppb for the latter. Because the inhibited enzyme can be reactivated when immediately treated with an oxime, the biosensor reactivation has been studied when 1,1′-trimethylene bis 4-formylpyridinium bromide dioxime (TMB-4) and pyridine 2-aldoxime methiodide (2-PAM) were used. TMB-4 resulted more effective. The comparison with the behavior of similar AChE based biosensors is also presented. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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17. Acetylcholinesterase biosensor based on single-walled carbon nanotubes—Co phtalocyanine for organophosphorus pesticides detection
- Author
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Ivanov, A.N., Younusov, R.R., Evtugyn, G.A., Arduini, F., Moscone, D., and Palleschi, G.
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ACETYLCHOLINESTERASE , *BIOSENSORS , *CARBON nanotubes , *ORGANOPHOSPHORUS compounds , *PESTICIDES , *DETECTORS , *CARBON electrodes , *OXIDATION - Abstract
Abstract: A simple and reliable technique has been developed for the construction of an amperometric acetylcholinesterase biosensor based on screen-printed carbon electrodes. For the first time, one-step modification using single-walled carbon nanotubes and Co phtalocyanine has been proposed to decrease the working potential and to increase the signal of thiocholine oxidation. The biosensor developed made it possible to detect 5–50ppb of paraoxon and 2–50ppb of malaoxon with detection limits of 3 and 2ppb, respectively (incubation 15min). The biosensor showed high reproducibility when measurements of the substrate and inhibitor were performed (R.S.D. about 1% and 2.5%, respectively). The reliability of the inhibition measurements was confirmed by testing spiked samples of sparkling and tape waters. [Copyright &y& Elsevier]
- Published
- 2011
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18. An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos
- Author
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Di Tuoro, D., Portaccio, M., Lepore, M., Arduini, F., Moscone, D., and Mita, D.G.
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- 2010
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19. Functionalized orthopaedic implant as pH electrochemical sensing tool for smart diagnosis of hardware infection.
- Author
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Fiore L, Mazzaracchio V, Gosti C, Duranti L, Vitiello R, Maccauro G, and Arduini F
- Abstract
In the orthopaedic surgery field, the use of medical implants to treat a patient's bone fracture is nowadays a common practice, nevertheless, it is associated with possible cases of infection. The consequent hardware infection can lead to implant failure and systemic infections, with prolonged hospitalization, time-consuming rehabilitation treatments, and extended antibiotic therapy. Hardware infections are strictly related to bacterial adhesion to the implant, leading to infection occurrence and consequent pH decreasing from physiological level to acid pH. Here, we demonstrate the new strategy to use an orthopaedic implant functionalized with iridium oxide film as the working electrode for the potentiometric monitoring of pH in hardware infection diagnosis. A functional investigation was focused on selecting the implant material, namely titanium, titanium alloy, and stainless steel, and the component, namely screws and implants. After selecting the titanium-based implant as the working electrode and a silver wire as the reference electrode in the final configuration of the smart sensing orthopaedic implant, a calibration curve was performed in standard solutions. An equation equal to y = (0.76 ± 0.02) - (0.068 ± 0.002) x , R
2 = 0.996, was obtained in the pH range of 4-8. Subsequently, hysteresis, interference, matrix effect, recovery study, and storage stability were investigated to test the overall performance of the sensing device, demonstrating the tremendous potential of electrochemical sensors to deliver the next generation of smart orthopaedic implants.- Published
- 2024
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20. Paper-Based Electrochemical (Bio)Sensors for the Detection of Target Analytes in Liquid, Aerosol, and Solid Samples.
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Colozza N, Mazzaracchio V, and Arduini F
- Abstract
The last decade has been incredibly fruitful in proving the multifunctionality of paper for delivering innovative electrochemical (bio)sensors. The paper material exhibits unprecedented versatility to deal with complex liquid matrices and facilitate analytical detection in aerosol and solid phases. Such remarkable capabilities are feasible by exploiting the intrinsic features of paper, including porosity, capillary forces, and its easy modification, which allow for the fine designing of a paper device. In this review, we shed light on the most relevant paper-based electrochemical (bio)sensors published in the literature so far to identify the smart functional roles that paper can play to bridge the gap between academic research and real-world applications in the biomedical, environmental, agrifood, and security fields. Our analysis aims to highlight how paper's multifarious properties can be artfully harnessed for breaking the boundaries of the most classical applications of electrochemical (bio)sensors.
- Published
- 2024
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21. Smartphone-assisted paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva.
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Fabiani L, Fiore L, Fillo S, D'Amore N, De Santis R, Lista F, and Arduini F
- Subjects
- Humans, Saliva, SARS-CoV-2, Immunoassay methods, Smartphone, Limit of Detection, Electrochemical Techniques methods, Electrodes, Biosensing Techniques methods, COVID-19 diagnosis
- Abstract
Herein, we developed a new waste solution-free paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva, by combining vertical and lateral flow. In detail, the device was constituted of a reservoir containing all reagents for the construction of the immunological chain onto the magnetic beads and a lateral flow holder which contained a polyester-based electrode, a magnet, and an adsorbent pad. The measurement was carried out by adding the saliva sample into the reservoir, followed by the addition of this solution in the hole present in the lateral flow holder. The successive additions of washing buffer and TMB solution in the lateral flow holder allowed the detection of N protein in saliva in the range of 0.06 to 4 µg/mL with a detection limit equal to 30 ng/mL. The analysis of several saliva samples with the sensing tool and the reference method, demonstrated the effectiveness of this device, being able to identify positive patients with high values of CT e.g. 35. This new configuration paves the way for the realization of any magnetic beads-based immunosystem without waste solution production, enlarging the application of paper-based devices., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)
- Published
- 2024
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22. Wireless real-time monitoring of oestradiol in sweat.
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Arduini F
- Published
- 2024
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23. Paper as smart support for bioreceptor immobilization in electrochemical paper-based devices.
- Author
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Seddaoui N, Colozza N, Gullo L, and Arduini F
- Subjects
- Molecularly Imprinted Polymers, Antibodies, Engineering, Electrochemical Techniques, Biosensing Techniques
- Abstract
The use of paper as a smart support in the field of electrochemical sensors has been largely improved over the last 15 years, driven by its outstanding features such as foldability and porosity, which enable the design of reagent and equipment-free multi-analysis devices. Furthermore, the easy surface engineering of paper has been used to immobilize different bioreceptors, through physical adsorption, covalent bonding, and electrochemical polymerization, boosting the fine customization of the analytical performances of paper-based biosensors. In this review, we focused on the strategies to engineer the surface of the paper for the immobilization of (bio)recognition elements (eg., enzymes, antibodies, DNA, molecularly imprinted polymers) with the overriding goal to develop accurate and reliable paper-based electrochemical biosensors. Furthermore, we highlighted how to take advantage of paper for designing smart configurations by integrating different analytical processes in an eco-designed analytical tool, starting from the immobilization of the (bio)receptor and the reagents, through a designed sample flow along the device, until the analyte detection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
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24. Paper card-like electrochemical platform as a smart point-of-care device for reagent-free glucose measurement in tears.
- Author
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Fiore L, Sinha A, Seddaoui N, di Biasio J, Ricci F, Stojanovic GM, and Arduini F
- Subjects
- Electrochemical Techniques, Glucose, Indicators and Reagents, Paper, Polyvinyl Chloride chemistry, Lubricant Eye Drops, Point-of-Care Systems
- Abstract
This communication describes the development of polyvinyl chloride electrochemical system in which a paper layer loaded with reagents is inserted into the device, demonstrating a new concept of a paper card-like pad for a reagent-free and easy measurement of the target analyte in solution. This device detects glucose in artificial tears in the range of 0.2-2 mM with a detection limit of 50 μM by simply adding the artificial tears to the paper card-like pad. The novel configuration goes beyond the state of the art, widening the application range of paper in the design of smart analytical devices.
- Published
- 2023
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25. A smart paper-based electrochemical sensor for reliable detection of iron ions in serum.
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Mazzaracchio V, Bagheri N, Chiara F, Fiore L, Moscone D, Roggero S, and Arduini F
- Subjects
- Gold chemistry, Limit of Detection, Electrodes, Electrochemical Techniques methods, Iron chemistry, Metal Nanoparticles
- Abstract
The fast-growing healthcare demand for user-friendly and affordable analytical tools is driving the efforts to develop reliable platforms for the customization of therapy based on individual health conditions. In this overall scenario, we developed a paper-based electrochemical sensor for the quantification of iron ions in serum as a cost-effective sensing tool for the correct supplement administration. In detail, the working electrode of the screen-printed device has been modified with a nanocomposite constituted of carbon black and gold nanoparticles with a drop-casting procedure. Square wave voltammetry has been adopted as an electrochemical technique. This sensor was further modified with Nafion for iron quantification in serum after sample treatment with trifluoroacetic acid. Under optimized conditions, iron ions have been detected with a LOD down to 0.05 mg/L and a linearity up to 10 mg/L in standard solution. The obtained results have been compared with reference methods namely commercial colorimetric assay and atomic absorption spectroscopy, obtaining a good correlation within the experimental errors. These results demonstrated the suitability of the developed paper-based sensor for future applications in precision medicine of iron-deficiency diseases., (© 2023. Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2023
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26. Nanomaterials and paper-based electrochemical devices: merging strategies for fostering sustainable detection of biomarkers.
- Author
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Caratelli V, Di Meo E, Colozza N, Fabiani L, Fiore L, Moscone D, and Arduini F
- Subjects
- Reproducibility of Results, Biomarkers, Cellulose, Nanostructures chemistry, Biosensing Techniques
- Abstract
In the last few decades, nanomaterials have made great advances in the biosensor field, thanks to their ability to enhance several key issues of biosensing analytical tools, namely, sensitivity, selectivity, robustness, and reproducibility. The recent trend of sustainability has boosted the progress of novel and eco-designed electrochemical paper-based devices to detect easily the target analyte(s) with high sensitivity in complex matrices. The huge attention given by the scientific community and industrial sectors to paper-based devices is ascribed to the numerous advantages of these cost-effective analytical tools, including the absence of external equipment for solution flow, thanks to the capillary force of paper, the fabrication of reagent-free devices, because of the loading of reagents on the paper, and the easy multistep analyses by using the origami approach. Besides these features, herein we highlight the multifarious aspects of the nanomaterials such as (i) the significant enlargement of the electroactive surface area as well as the area available for the desired chemical interactions, (ii) the capability of anchoring biorecognition elements on the electrode surface on the paper matrix, (iii) the improvement of the conductivity of the cellulose matrix, (iv) the functionality of photoelectrochemical properties within the cellulose matrix, and (v) the improvement of electrochemical capabilities of conductive inks commonly used for electrode printing on the paper support, for the development of a new generation of paper-based electrochemical biosensors applied in the biomedical field. The state of the art over the last ten years has been analyzed highlighting the various functionalities that arise from the integration of nanomaterials with paper-based electrochemical biosensors for the detection of biomarkers.
- Published
- 2022
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27. Multiplexed sensing techniques for cardiovascular disease biomarkers - A review.
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Mani V, Durmus C, Khushaim W, Ferreira DC, Timur S, Arduini F, and Salama KN
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- Biomarkers, Humans, Prospective Studies, Biosensing Techniques methods, Cardiovascular Diseases diagnosis, Nanostructures
- Abstract
Cardiovascular diseases (CVDs) are the number one cause of death worldwide, taking 17.9 million lives each year. The rapid, sensitive, and accurate determination of cardiac biomarkers is vital for the timely diagnosis of CVDs. For accurate diagnosis, dependence on a single biomarker is unreliable because each one has also been linked to other diseases. To overcome this problem, the multiplexed determination of two or more markers has emerged as a promising alternative to single-marker analysis. Over the last 5 years, research interest in the development of biosensors for targeting multiple cardiac markers has increased. In this study, we critically reviewed the various multiplexed biosensing approaches reported during the last 5 years, categorizing them by signal readouts. Prospective detection configurations, capture probes, electrode design strategies, electrode types, nanomaterials, reporter tags, and assay types were reviewed, tabulated, and critically discussed. Then, their advantages and limitations were highlighted. For each category, we provided our perspective as well as the overall critical discussion. Lastly, we summarized potential commercial multiplexed cardiac biosensors and commented on the challenges and future prospects for such sensors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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28. Mask assistance to colorimetric sniffers for detection of Covid-19 disease using exhaled breath metabolites.
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Bordbar MM, Samadinia H, Hajian A, Sheini A, Safaei E, Aboonajmi J, Arduini F, Sharghi H, Hashemi P, Khoshsafar H, Ghanei M, and Bagheri H
- Abstract
According to World Health Organization reports, large numbers of people around the globe have been infected or died for Covid-19 due to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Researchers are still trying to find a rapid and accurate diagnostic method for revealing infected people by low viral load with the overriding goal of effective diagnostic management. Monitoring the body metabolic changes is known as an effective and inexpensive approach for the evaluation of the infected people. Here, an optical sniffer is introduced to detect exhaled breath metabolites of patients with Covid-19 (60 samples), healthy humans (55 samples), and cured people (15 samples), providing a unique color pattern for differentiation between the studied samples. The sniffer device is installed on a thin face mask, and directly exposed to the exhaled breath stream. The interactions occurring between the volatile compounds and sensing components such as porphyrazines, modified organic dyes, porphyrins, inorganic complexes, and gold nanoparticles allowing for the change of the color, thus being tracked as the sensor responses. The assay accuracy for the differentiation between patient, healthy and cured samples is calculated to be in the range of 80%-84%. The changes in the color of the sensor have a linear correlation with the disease severity and viral load evaluated by rRT-PCR method. Interestingly, comorbidities such as kidney, lung, and diabetes diseases as well as being a smoker may be diagnosed by the proposed method. As a powerful detection device, the breath sniffer can replace the conventional rapid test kits for medical applications., (© 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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29. An origami paper-based electrochemical biosensing platform for quality control of agri-food waste in the valorization strategy.
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Colozza N, Di Meo E, Mucaria A, Moscone D, and Arduini F
- Subjects
- Food, Glucose, Paper, Quality Control, Glucosinolates, Refuse Disposal
- Abstract
The increasing demand for food and the need for a sustainability vision in the agri-food sector have boosted novel approaches for food management, enhancing the valorization of wastes and by-products belonging to the food industry. Herein, we present a novel paper-based origami device to assess the amount of both glucosinolate and glucose in a food waste product belonging to Brassicaceae plants, to evaluate the quality value and the correct management of waste samples. The device has been designed as an origami paper-based platform constituted of two paper-based biosensors to work synergistically in a multiplexed detection. In detail, a monoenzymatic biosensor and a bienzymatic biosensor were configured for the detection of glucose and glucosinolates, respectively, using filter paper pads preloaded with glucose oxidase and/or myrosinase. To complete the paper-based platform, the enzyme-preloaded pads were combined with office paper-based electrodes modified with Carbon black/Prussian Blue nanoparticles for the measurement of enzymatic by-product at a low applied potential (i.e., 0 V versus Ag/AgCl). Overall, this paper-based platform measured glucose and glucosinolate (i.e., sinigrin) with a linear range up to 2.5 and 1.5 mM, and detection limits of 0.05 and 0.07 mM, respectively. The repeatability corresponded to an RSD% equal to 5% by testing 10 mM of glucose, and 10% by testing 1 mM of sinigrin. The accuracy of the developed multiplex device was evaluated by recovery studies at two different levels of sinigrin, i.e., 0.25 and 0.5 mM, obtaining recoveries values equal to (111 ± 3) % and (86 ± 1) %, respectively. The multiplex detection of both glucose and glucosinolate in Brassicaceae samples evaluates the quality values of the waste sample, ensuring the quality of the re-used food product waste by using an eco-designed analytical tool. The combination of paper-based devices for quality control of food waste with the re-use of these food products represents a sustainable approach that perfectly matches sustainable agrifood practices as well as the overall approach of the circular economy., (© 2022. The Author(s).)
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- 2022
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30. A Proof-of-Concept Electrochemical Cytosensor Based on Chlamydomonas reinhardtii Functionalized Carbon Black Screen-Printed Electrodes: Detection of Escherichia coli in Wastewater as a Case Study.
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Antonacci A, Arduini F, Attaallah R, Amine A, Giardi MT, and Scognamiglio V
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- Carbon chemistry, Electrodes, Escherichia coli, Oxygen, Soot, Wastewater, Chlamydomonas reinhardtii, Escherichia coli Infections
- Abstract
Herein, we report a proof-of-concept algal cytosensor for the electrochemical quantification of bacteria in wastewater, exploiting the green photosynthetic alga Chlamydomonas reinhardtii immobilized on carbon black (CB) nanomodified screen-printed electrodes. The CB nanoparticles are used as nanomodifiers, as they are able to sense the oxygen produced by the algae and thus the current increases when algae are exposed to increasing concentrations of bacteria. The sensor was tested on both standard solutions and real wastewater samples for the detection Escherichia coli in a linear range of response from 100 to 2000 CFU/100 mL, showing a limit of detection of 92 CFU/100 mL, in agreement with the maximum E. coli concentration established by the Italian law for wastewater (less than 5000 CFU/100 mL). This bacterium was exploited as a case study target of the algal cytosensor to demonstrate its ability as an early warning analytical system to signal heavy loads of pathogens in waters leaving the wastewater treatment plants. Indeed, the cytosensor is not selective towards E. coli but it is capable of sensing all the bacteria that induce the algae oxygen evolution by exploiting the effect of their interaction. Other known toxicants, commonly present in wastewater, were also analyzed to test the cytosensor selectivity, with any significant effect, apart from atrazine, which is a specific target of the D1 protein of the Chlamydomonas photosystem II. However, the latter can also be detected by chlorophyll fluorescence simultaneously to the amperometric measurements. The matrix effect was evaluated, and the recovery values were calculated as 105 ± 8, 83 ± 7, and 88 ± 7% for 1000 CFU/100 mL of E. coli in Lignano, San Giorgio, and Pescara wastewater samples, respectively.
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- 2022
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31. A paper-based electrochemical device for the detection of pesticides in aerosol phase inspired by nature: A flower-like origami biosensor for precision agriculture.
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Caratelli V, Fegatelli G, Moscone D, and Arduini F
- Subjects
- Aerosols, Agriculture, Butyrylcholinesterase, Biosensing Techniques methods, Pesticides analysis
- Abstract
Pesticides are largely used at worldwide level to improve food production, fulfilling the needs of the global population which is increasing year by year. Although pesticides are beneficial for crop production, their extensive use has serious consequences for the pollution of the produced food as well as for soil and groundwaters. Indeed, it is reported that 50% of sprayed pesticides reach different destinations other than their target species, including soil, surface waters, and groundwaters. For this reason, we developed a flower-like origami paper-based device for pesticides detection in aerosol phase for precision agriculture. In detail, the paper-based electrochemical platform detects paraoxon, 2,4-dichlorophenoxyacetic acid, and glyphosate at ppb levels by measuring their inhibitory activity towards three different enzymes namely butyrylcholinesterase, alkaline phosphatase, and peroxidase enzyme, respectively. This integrated electrochemical device is composed of three office paper-based screen-printed electrodes and filter paper-based pads loaded with enzymes and enzymatic substrates. The pesticide detection is carried out by measuring through chronoamperometric technique the initial and residual enzymatic activity by using a smartphone-assisted potentiostat and evaluating the percentage of inhibition, proportional to the amount of aerosolized pesticides. This paper-based device was able to detect the three classes of pesticides in aerosol phase with limits of detection equal to 30 ppb, 10 ppb, and 2 ppb, respectively for 2,4-D, glyphosate, and paraoxon., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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32. Paper-based immunoassay based on 96-well wax-printed paper plate combined with magnetic beads and colorimetric smartphone-assisted measure for reliable detection of SARS-CoV-2 in saliva.
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Fabiani L, Mazzaracchio V, Moscone D, Fillo S, De Santis R, Monte A, Amatore D, Lista F, and Arduini F
- Subjects
- Colorimetry, Humans, Immunoassay, Magnetic Phenomena, Nasopharynx, Reproducibility of Results, SARS-CoV-2, Saliva, Smartphone, Specimen Handling, Biosensing Techniques, COVID-19
- Abstract
Coronavirus disease 2019 (COVID-19) has been recognized as a global pandemic outbreak, opening the most severe socio-economic crisis since World War II. Different scientific activities have been emerged in this global scenario, including the development of innovative analytical tools to measure nucleic acid, antibodies, and antigens in the nasopharyngeal swab, serum, and saliva for prompt identification of COVID-19 patients and to evaluate the immune response to the vaccine. The detection of SARS-CoV-2 in saliva remains a challenge for the lack of sufficient sensitivity. To address this issue, we developed a novel paper-based immunoassay using magnetic beads to support the immunological chain and 96-well wax-printed paper plate as a platform for color visualization by using a smartphone combined with Spotxel free-charge app. To assess the reliability of the measurement of SARS-CoV-2 in saliva, untreated saliva was used as a specimen and the calibration curve demonstrated a dynamic range up to 10 μg/mL, with a detection limit equal to 0.1 μg/mL. The effectiveness of this sustainable analytical tool in saliva was evaluated by comparing the data with the nasopharyngeal swab specimens sampled by the same patients and tested with Real-Time PCR reference method, founding 100% of agreement, even in the case of high Cycle Threshold (CT) numbers (low viral load). Furthermore, the positive saliva samples were characterized by the next-generation sequencing method, demonstrating the capability to detect the Delta variant, which is actually (July 2021) the most relevant variant of concern., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2022
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33. Smartphone-assisted electrochemical sensor for reliable detection of tyrosine in serum.
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Fiore L, De Lellis B, Mazzaracchio V, Suprun E, Massoud R, Goffredo BM, Moscone D, and Arduini F
- Subjects
- Electrodes, Limit of Detection, Soot, Tyrosine, Electrochemical Techniques, Smartphone
- Abstract
Point-of-care devices have attracted a huge interest by the scientific community because of the valuable potentiality for rapid diagnosis and precision medicine through cost-effective and easy-to-use devices for on-site measurement by unskilled personnel. Herein, we reported a smartphone-assisted electrochemical device consisted of a screen-printed electrode modified with carbon black nanomaterial and a commercially available smartphone potentiostat i.e. EmStat3 Blue, for sensitive detection of tyrosine. Once optimized the conditions, tyrosine was detected in standard solutions by square wave voltammetry, achieving a linear range comprised between 30 and 500 μM, with a detection limit equal to 4.4 μM. To detect tyrosine in serum, the interference of another amino acid i.e. tryptophan was hindered using a sample treatment with an extraction cartridge. The agreement of results analyzing serum samples with HPLC reference method and with the developed smart sensing system demonstrated the suitability of this smartphone-assisted sensing tool for cost-effective and rapid analyses of tyrosine in serum samples., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2022
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34. Pressure on the Health-Care System and Intensive Care Utilization During the COVID-19 Outbreak in the Lombardy Region of Italy: A Retrospective Observational Study in 43,538 Hospitalized Patients.
- Author
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Trentini F, Marziano V, Guzzetta G, Tirani M, Cereda D, Poletti P, Piccarreta R, Barone A, Preziosi G, Arduini F, Della Valle PG, Zanella A, Grosso F, Del Castillo G, Castrofino A, Grasselli G, Melegaro A, Piatti A, Andreassi A, Gramegna M, Ajelli M, and Merler S
- Subjects
- Adult, Age Factors, Aged, Aged, 80 and over, COVID-19 mortality, Comorbidity, Humans, Italy epidemiology, Middle Aged, Pandemics, Retrospective Studies, Risk Factors, SARS-CoV-2, Sex Factors, Time Factors, COVID-19 epidemiology, COVID-19 therapy, Delivery of Health Care statistics & numerical data, Intensive Care Units statistics & numerical data
- Abstract
During the spring of 2020, the coronavirus disease 2019 (COVID-19) epidemic caused an unprecedented demand for intensive-care resources in the Lombardy region of Italy. Using data on 43,538 hospitalized patients admitted between February 21 and July 12, 2020, we evaluated variations in intensive care unit (ICU) admissions and mortality over the course of 3 periods: the early phase of the pandemic (February 21-March 13), the period of highest pressure on the health-care system (March 14-April 25, when numbers of COVID-19 patients exceeded prepandemic ICU bed capacity), and the declining phase (April 26-July 12). Compared with the early phase, patients aged 70 years or more were less often admitted to an ICU during the period of highest pressure on the health-care system (odds ratio (OR) = 0.47, 95% confidence interval (CI): 0.41, 0.54), with longer ICU delays (incidence rate ratio = 1.82, 95% CI: 1.52, 2.18) and lower chances of dying in the ICU (OR = 0.47, 95% CI: 0.34, 0.64). Patients under 56 years of age had more limited changes in the probability of (OR = 0.65, 95% CI: 0.56, 0.76) and delay to (incidence rate ratio = 1.16, 95% CI: 0.95, 1.42) ICU admission and increased mortality (OR = 1.43, 95% CI: 1.00, 2.07). In the declining phase, all quantities decreased for all age groups. These patterns may suggest that limited health-care resources during the peak phase of the epidemic in Lombardy forced a shift in ICU admission criteria to prioritize patients with higher chances of survival., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Johns Hopkins Bloomberg School of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2022
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35. Robot-assisted rehabilitation for children with neurological disabilities: Results of the Italian consensus conference CICERONE.
- Author
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Castelli E, Beretta E, De Tanti A, Arduini F, Biffi E, Colazza A, Di Pede C, Guzzetta A, Lucarini L, Maghini I, Mandalà M, Nespoli M, Pavarelli C, Policastro F, Polverelli M, Rossi A, Sgandurra G, Boldrini P, Bonaiuti D, Mazzoleni S, Posteraro F, Benanti P, Draicchio F, Falabella V, Galeri S, Gimigliano F, Grigioni M, Mazzon S, Molteni F, Morone G, Petrarca M, Picelli A, Senatore M, Turchetti G, and Saviola D
- Subjects
- Child, Humans, Gait, Robotics methods, Nervous System Diseases rehabilitation, Disabled Children rehabilitation
- Abstract
Background: The use of robotic technologies in pediatric rehabilitation has seen a large increase, but with a lack of a comprehensive framework about their effectiveness., Objective: An Italian Consensus Conference has been promoted to develop recommendations on these technologies: definitions and classification criteria of devices, indications and limits of their use in neurological diseases, theoretical models, ethical and legal implications. In this paper, we present the results for the pediatric age., Methods: A systematic search on Cochrane Library, PEDro and PubMed was performed. Papers published up to March 1st, 2020, in English, were included and analyzed using the methodology of the Centre for Evidence-Based Medicine in Oxford, AMSTAR2 and PEDro scales for systematic reviews and RCT, respectively., Results: Some positives aspects emerged in the area of gait: an increased number of children reaching the stance, an improvement in walking distance, speed and endurance. Critical aspects include the heterogeneity of the studied cases, measurements and training protocols., Conclusion: Many studies demonstrate the benefits of robotic training in developmental age. However, it is necessary to increase the number of trials to achieve greater homogeneity between protocols and to confirm the effectiveness of pediatric robotic rehabilitation.
- Published
- 2022
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36. Vertical-Flow Paper Sensor for On-Site and Prompt Evaluation of Chloride Contamination in Concrete Structures.
- Author
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Colozza N, Tazzioli S, Sassolini A, Agosta L, di Monte MG, Hermansson K, and Arduini F
- Abstract
Corrosion occurring in reinforced concrete has turned into a primary concern of the current century, concrete being the most ubiquitous and predominant material used in the construction industry. Among the many interrelated processes that trigger corrosion of metallic reinforcements, the penetration of chloride ions into the concrete matrix is the most insidious threat. Herein, we developed the first electrochemical device entirely made of paper that allows for the direct, prompt, and noninvasive evaluation of free chloride ion contamination in concrete-based constructions. Our device is based on a three-layer wax-modified filter paper, consisting of two Ag/AgCl screen-printed electrodes that are interfaced by a junction pad in a sandwich-like configuration. Filter paper allows for generating a vertical-flow potentiometric device capable of measuring the electrochemical potential between two solutions containing different concentrations of chloride ions, which are separately drop-cast on the top and bottom layers. After demonstrating the analytical performance of the device, the same principle was applied to the evaluation of the chloride contents in different concrete samples, exploiting paper as a suitable interfacing material for potentiometric measurements on the cement solid surface. Laboratory-prepared concrete samples with known chloride contents were first assessed, and then, the paper-based vertical-flow device was applied to real concrete structures at the Giacomo Manzù Museum (Ardea, Italy) for the evaluation of chloride contamination caused by the proximity to the seaside. The capability of our device to provide timely warning of the risk conditions of concrete-based artifacts was demonstrated.
- Published
- 2021
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37. Modulating the Enantiodiscrimination Features of Inherently Chiral Selectors by Molecular Design: A HPLC and Voltammetry Study Case with Atropisomeric 2,2'-Biindole-Based Monomers and Oligomer Films.
- Author
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Scapinello L, Grecchi S, Rossi S, Arduini F, Arnaboldi S, Penoni A, Cirilli R, Romana Mussini P, and Benincori T
- Subjects
- Circular Dichroism, Electrodes, Molecular Structure, Stereoisomerism, Chromatography, High Pressure Liquid
- Abstract
A family of inherently chiral electroactive selectors based on the 2,2'-biindole atropisomeric scaffold, of easy synthesis and modulable functional properties, is studied in cascade in two enantioselection contexts. They are at first investigated as probes in enantioselective HPLC, studying molecular structure and temperature effects, and achieving very efficient semipreparative enantioseparation. The enantiomers thus obtained, of remarkable chiroptical features (optical rotation as well as circular dichroism), are successfully applied as selectors in chiral voltammetry in different media for discrimination of the enantiomers of chiral electroactive probes, either by conversion into enantiopure electroactive electrode surfaces by electrooligomerization on glassy carbon substrate (the two monomers with shorter alkyl chains), or as chiral additive in achiral ionic liquid (the monomer with longest alkyl chains). Discrimination is conveniently and reproducibly achieved in terms of significant potential differences for the two enantiomers, specularly inverting either probe or selector configuration. In one case successful discrimination is also observed with the two probe enantiomers concurrently present, either as racemate or with enantiomeric excesses, neatly accounted for by the peak current ratios., (© 2021 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.)
- Published
- 2021
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38. Origami Paper-Based Electrochemical (Bio)Sensors: State of the Art and Perspective.
- Author
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Colozza N, Caratelli V, Moscone D, and Arduini F
- Subjects
- Electrodes, Biosensing Techniques, Electrochemical Techniques, Paper
- Abstract
In the last 10 years, paper-based electrochemical biosensors have gathered attention from the scientific community for their unique advantages and sustainability vision. The use of papers in the design the electrochemical biosensors confers to these analytical tools several interesting features such as the management of the solution flow without external equipment, the fabrication of reagent-free devices exploiting the porosity of the paper to store the reagents, and the unprecedented capability to detect the target analyte in gas phase without any sampling system. Furthermore, cost-effective fabrication using printing technologies, including wax and screen-printing, combined with the use of this eco-friendly substrate and the possibility of reducing waste management after measuring by the incineration of the sensor, designate these type of sensors as eco-designed analytical tools. Additionally, the foldability feature of the paper has been recently exploited to design and fabricate 3D multifarious biosensors, which are able to detect different target analytes by using enzymes, antibodies, DNA, molecularly imprinted polymers, and cells as biocomponents. Interestingly, the 3D structure has recently boosted the self-powered paper-based biosensors, opening new frontiers in origami devices. This review aims to give an overview of the current state origami paper-based biosensors, pointing out how the foldability of the paper allows for the development of sensitive, selective, and easy-to-use smart and sustainable analytical devices.
- Published
- 2021
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39. Multi-array wax paper-based platform for the pre-concentration and determination of silver ions in drinking water.
- Author
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Bagheri N, Cinti S, Nobile E, Moscone D, and Arduini F
- Abstract
In this work, a wax-patterned chromatographic paper has been utilized as a holistic platform to 1) synthesize Prussian Blue Nanoparticles (sensing species), 2) load the reagents for the assay, 3) concentrate the sample through multistep, and 4) visualize the determination of silver ions. Waters are continuously affected by changes in the composition, thus the utilization of reagent-free analytical tools is of huge interest for smart drinking water monitoring. Herein, we report the characterization and application of a multi-array paper-based platform for the colorimetric determination of silver ions based on the conversion from Prussian Blue to its silver-based analogue, namely Ag
4 [Fe(CN)6 ]. In particular, the platform highlights the increase of sensitivity due to paper pre-concentration of sample, that can be easily adapted to the analytical necessities. Within the proposed experimental setup, Ag+ is visualized down to a detection limit of 0.9 μM, with high repeatability and satisfactory recoveries in the range comprised between 90 and 113%., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2021
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40. State of the Art on the SARS-CoV-2 Toolkit for Antigen Detection: One Year Later.
- Author
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Fabiani L, Caratelli V, Fiore L, Scognamiglio V, Antonacci A, Fillo S, De Santis R, Monte A, Bortone M, Moscone D, Lista F, and Arduini F
- Subjects
- Biosensing Techniques, COVID-19 immunology, Electrochemical Techniques, Humans, Reagent Kits, Diagnostic, Sensitivity and Specificity, Antigens, Viral analysis, COVID-19 diagnosis, COVID-19 Testing methods, SARS-CoV-2 immunology
- Abstract
The recent global events of COVID-19 in 2020 have alerted the world to the risk of viruses and their impacts on human health, including their impacts in the social and economic sectors. Rapid tests are urgently required to enable antigen detection and thus to facilitate rapid and simple evaluations of contagious individuals, with the overriding goal to delimitate spread of the virus among the population. Many efforts have been achieved in recent months through the realization of novel diagnostic tools for rapid, affordable, and accurate analysis, thereby enabling prompt responses to the pandemic infection. This review reports the latest results on electrochemical and optical biosensors realized for the specific detection of SARS-CoV-2 antigens, thus providing an overview of the available diagnostics tested and marketed for SARS-CoV-2 antigens as well as their pros and cons.
- Published
- 2021
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41. A paper-based electrochemical sensor for H 2 O 2 detection in aerosol phase: Measure of H 2 O 2 nebulized by a reconverted ultrasonic aroma diffuser as a case of study.
- Author
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Fiore L, Mazzaracchio V, Galloni P, Sabuzi F, Pezzola S, Matteucci G, Moscone D, and Arduini F
- Abstract
The outbreak of COVID-19 is caused by high contagiousness and rapid spread of SARS-CoV-2 virus between people when an infected person is in close contact with another one. In this overall scenario, the disinfection processes have been largely improved. For instance, some countries have approved no-touch technologies by vaporizing disinfectants such as hydrogen peroxide, with the overriding goal to boost the safety of the places. In the era of sustainability, we designed an electrochemical paper-based device for the assessment of hydrogen peroxide nebulized by a cost-effective ultrasonic aroma diffuser. The paper-based sensor was fabricated by modifying via drop-casting a filter paper-based screen-printed electrode with a dispersion of carbon black-Prussian Blue nanocomposite, to assess the detection of hydrogen peroxide at -0.05 V vs Ag/AgCl. The use of paper-based modified screen-printed electrode loaded with phosphate buffer allowed for monitoring the concentration of hydrogen peroxide in aerosol, without any additional sampling instrument to capture the nebulized solution of hydrogen peroxide at a concentration up to 7% w/w. Hydrogen peroxide, a reconverted ultrasonic aroma diffuser, and the paper-based electrochemical sensor assisted by smartphone have demonstrated how different low-cost technologies are able to supply an useful and cost-effective solution for disinfection procedures., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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42. Paper-based electrochemical peptide sensor for on-site detection of botulinum neurotoxin serotype A and C.
- Author
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Caratelli V, Fillo S, D'Amore N, Rossetto O, Pirazzini M, Moccia M, Avitabile C, Moscone D, Lista F, and Arduini F
- Subjects
- Animals, Gold, Limit of Detection, Mice, Peptides, Serogroup, Biosensing Techniques, Botulinum Toxins, Type A, Metal Nanoparticles
- Abstract
Botulinum neurotoxins (BoNTs) produced by soil bacterium Clostridium botulinum are cause of botulism and listed as biohazard agents, thus rapid screening assays are needed for taking the correct countermeasures in a timely fashion. The gold standard method relies on the mouse lethality assay with a lengthy analysis time, i.e., 2-5 days, hindering the prompt management of food safety and medical diagnosis. Herein, we propose the first paper-based antibody-free sensor for reliable and rapid detection of BoNT/A and BoNT/C, exploiting their cleavage capability toward a synthetic peptide able to mimic the natural substrate SNAP-25. The peptide is labelled with the electroactive molecule methylene blue and immobilized on the paper-based electrode modified with gold nanoparticles. Because BoNT/A and BoNT/C can cleave the peptide with the removal of methylene blue from electrode surface, the presence of these neurotoxins in the sample leads to a signal decrease proportional to BoNT amount. The biosensor developed with the selected peptide and combined with smartphone assisted potentiostat is able to detect both BoNT/A and BoNT/C with a linearity up to 1 nM and a detection limit equal to 10 pM. The applicability of this biosensor was evaluated with spiked samples of orange juice, obtaining recovery values equal to 104 ± 6% and 98 ± 9% for 1 nM and 0.5 nM of BoNT/A, respectively., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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43. A dual electro-optical biosensor based on Chlamydomonas reinhardtii immobilised on paper-based nanomodified screen-printed electrodes for herbicide monitoring.
- Author
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Antonacci A, Attaallah R, Arduini F, Amine A, Giardi MT, and Scognamiglio V
- Subjects
- Drinking Water, Ecosystem, Environmental Monitoring, Immobilization methods, Insecticides, Nanostructures, Soot, Biosensing Techniques, Chlamydomonas reinhardtii, Electrodes, Enzymes, Immobilized, Herbicides
- Abstract
The indiscriminate use of herbicides in agriculture contributes to soil and water pollution, with important endangering consequences on the ecosystems. Among the available analytical systems, algal biosensors have demonstrated to be valid tools thanks to their high sensitivity, cost-effectiveness, and eco-design. Herein, we report the development of a dual electro-optical biosensor for herbicide monitoring, based on Chlamydomonas reinhardtii whole cells immobilised on paper-based screen-printed electrodes modified with carbon black nanomaterials. To this aim, a systematic study was performed for the selection and characterisation of a collection among 28 different genetic variants of the alga with difference response behaviour towards diverse herbicide classes. Thus, CC125 strain was exploited as case study for the study of the analytical parameters. The biosensor was tested in standard solutions and real samples, providing high sensitivity (detection limit in the pico/nanomolar), high repeatability (RSD of 5% with n = 100), long lasting working (10 h) and storage stability (3 weeks), any interference in the presence of heavy metals and insecticides, and low matrix effect in drinking water and moderate effect in surface one.
- Published
- 2021
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44. Paper-based electrochemical sensor for on-site detection of the sulphur mustard.
- Author
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Colozza N, Kehe K, Popp T, Steinritz D, Moscone D, and Arduini F
- Subjects
- Electrochemical Techniques, Electrodes, Hydrogen Peroxide, Limit of Detection, Biosensing Techniques, Mustard Gas, Nanoparticles
- Abstract
Herein, we report a novel paper-based electrochemical sensor for on-site detection of sulphur mustards. This sensor was conceived combining office paper-based electrochemical sensor with choline oxidase enzyme to deliver a sustainable sensing tool. The mustard agent detection relies on the evaluation of inhibition degree of choline oxidase, which is reversibly inhibited by sulphur mustards, by measuring the enzymatic by-product H
2 O2 in chronoamperometric mode. A nanocomposite constituted of Prussian Blue nanoparticles and Carbon Black was used as working electrode modifier to improve the electroanalytical performances. This bioassay was successfully applied for the measurement of a sulphur mustard, Yprite, obtaining a detection limit in the millimolar range (LOD = 0.9 mM). The developed sensor, combined with a portable and easy-to-use instrumentation, can be applied for a fast and cost-effective detection of sulphur mustards.- Published
- 2021
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45. Electroanalytical Sensor Based on Gold-Nanoparticle-Decorated Paper for Sensitive Detection of Copper Ions in Sweat and Serum.
- Author
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Bagheri N, Mazzaracchio V, Cinti S, Colozza N, Di Natale C, Netti PA, Saraji M, Roggero S, Moscone D, and Arduini F
- Subjects
- Copper, Gold, Ions, Sweat, Biosensing Techniques, Metal Nanoparticles
- Abstract
The growth of (bio)sensors in analytical chemistry is mainly attributable to the development of affordable, effective, portable, and user-friendly analytical tools. In the field of sensors, paper-based devices are gaining a relevant position for their outstanding features including foldability, ease of use, and instrument-free microfluidics. Herein, a multifarious use of filter paper to detect copper ions in bodily fluids is reported by exploiting this eco-friendly material to (i) synthesize AuNPs without the use of reductants and/or external stimuli, (ii) print the electrodes, (iii) load the reagents for the assay, (iv) filter the gross impurities, and (v) preconcentrate the target analyte. Copper ions were detected down to 3 ppb with a linearity up to 400 ppb in standard solutions. The applicability in biological matrices, namely, sweat and serum, was demonstrated by recovery studies and by analyzing these biofluids with the paper-based platform and the reference method (atomic absorption spectroscopy), demonstrating satisfactory accuracy of the novel eco-designed analytical tool.
- Published
- 2021
- Full Text
- View/download PDF
46. Medium-distance affordable, flexible and wireless epidermal sensor for pH monitoring in sweat.
- Author
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Mazzaracchio V, Fiore L, Nappi S, Marrocco G, and Arduini F
- Subjects
- Electrodes, Hydrogen-Ion Concentration, Potentiometry, Epidermis, Sweat
- Abstract
In the last decade, wearable sensors have gained a key role on biomedical research field for reliable health state monitoring. A wide plethora of physics marker sensors is already commercially available, including activity tracker, heart rate devices, and fitness smartwatch. On the contrary, wearable and epidermal sensors for chemical biomarker monitoring in several biofluids are not ready yet. Herein, we report a wireless and flexible epidermal device for pH monitoring in sweat, fabricated by encompassing a screen-printed potentiometric sensor, an integrated circuit, and antenna embedded onto the same Kapton substrate. An iridium oxide film was electrodeposited onto the graphite working electrode providing the pH sensitive layer, while the integrated circuit board allows for data acquisition and storing. Furthermore, a radio frequency identification antenna surrounding the entire system enables data transmission to an external reader up to nearly 2 m in the most favourable case. The potentiometric sensor was firstly characterised by cyclic voltammetry experiments, then the iridium oxide electrodeposition procedure was optimised. Next, the sensor was tested toward pH detection in buffer solutions with a near-Nernstian response equal to -0.079 ± 0.002 V for unit of pH. Interference studies of common sweat ions, including Na
+ , K+ and Cl- , showed any influence on the pH sensor response. Finally, the integrated epidermal device was tested for real-time on-body pH sweat monitoring during a running activity. Data recorded for a running subject were wireless transmitted to an external receiver, showing a pH value close to 5.5, in agreement with value obtained by pH-meter reference measurement., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2021
- Full Text
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47. Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS-CoV-2 detection in saliva.
- Author
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Fabiani L, Saroglia M, Galatà G, De Santis R, Fillo S, Luca V, Faggioni G, D'Amore N, Regalbuto E, Salvatori P, Terova G, Moscone D, Lista F, and Arduini F
- Subjects
- COVID-19, COVID-19 Testing, Coronavirus Nucleocapsid Proteins, Electrochemical Techniques instrumentation, Electrodes, Equipment Design, Humans, Immunoassay instrumentation, Magnets chemistry, Nucleocapsid Proteins analysis, Pandemics, Phosphoproteins, SARS-CoV-2, Sensitivity and Specificity, Soot chemistry, Spike Glycoprotein, Coronavirus analysis, Betacoronavirus isolation & purification, Biosensing Techniques instrumentation, Clinical Laboratory Techniques, Coronavirus Infections diagnosis, Pneumonia, Viral diagnosis, Saliva virology
- Abstract
The diffusion of novel SARS-CoV-2 coronavirus over the world generated COVID-19 pandemic event as reported by World Health Organization on March 2020. The huge issue is the high infectivity and the absence of vaccine and customised drugs allowing for hard management of this outbreak, thus a rapid and on site analysis is a need to contain the spread of COVID-19. Herein, we developed an electrochemical immunoassay for rapid and smart detection of SARS-CoV-2 coronavirus in saliva. The electrochemical assay was conceived for Spike (S) protein or Nucleocapsid (N) protein detection using magnetic beads as support of immunological chain and secondary antibody with alkaline phosphatase as immunological label. The enzymatic by-product 1-naphtol was detected using screen-printed electrodes modified with carbon black nanomaterial. The analytical features of the electrochemical immunoassay were evaluated using the standard solution of S and N protein in buffer solution and untreated saliva with a detection limit equal to 19 ng/mL and 8 ng/mL in untreated saliva, respectively for S and N protein. Its effectiveness was assessed using cultured virus in biosafety level 3 and in saliva clinical samples comparing the data using the nasopharyngeal swab specimens tested with Real-Time PCR. The agreement of the data, the low detection limit achieved, the rapid analysis (30 min), the miniaturization, and portability of the instrument combined with the easiness to use and no-invasive sampling, confer to this analytical tool high potentiality for market entry as the first highly sensitive electrochemical immunoassay for SARS-CoV-2 detection in untreated saliva., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
48. Narrative review of multiparametric ultrasound in parotid gland evaluation.
- Author
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Martino M, Fodor D, Fresilli D, Guiban O, Rubini A, Cassoni A, Ralli M, De Vincentiis C, Arduini F, Celletti I, Pacini P, Polti G, Polito E, Greco A, Valentini V, Sorrenti S, D'Andrea V, Masciocchi C, Barile A, and Cantisani V
- Abstract
Disorders affecting parotid gland represent a heterogeneous group comprising congenital, inflammatory and neoplastic diseases which show a focal or diffuse pattern of appearance. The differentiation of neoplastic from non-neoplastic conditions of parotid glands is pivotal for the diagnostic imaging. Frequently there is evidence of overlapping between the clinical and the imaging appearance of the various pathologies. The parotid gland is also often object of study with the combination of different techniques [ultrasound-computed tomography-magnetic resonance imaging (US-CT-MRI), ex.]. Compared to other dominant methods of medical imaging, US has several advantages providing images in real-time at lower cost, and without harmful use of ionizing radiation and of contrast enhancement. B-mode US, and the microvascular pattern color Doppler are usually used as first step evaluation of parotid lesions. Elastography and contrast-enhanced US (CEUS) has opened further possible perspectives to improve the differentiation between benign and malignant parotid lesions. The characterization of the parotid tumors plays a crucial role for their treatment planning and for the prediction of possible surgical complications. We present, here an updated review of the most recurrent pathologies of parotid gland focusing on the diagnostic power of multiparametric US including CEUS and ultrasound elastography (USE); limitations, advantages and the main key-points will be presented., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/gs-20-530). The series “Multimodality Advanced Imaging and Intervention in Gland Diseases” was commissioned by the editorial office without any funding or sponsorship. AB serves as an unpaid editorial board member of Gland Surgery from Jun 2018 to May 2022 and served as the unpaid Guest Editor of the series. VC reports Lecturer fee from Bracco, Samsung and Toshiba. The other authors have no other conflicts of interest to declare., (2020 Gland Surgery. All rights reserved.)
- Published
- 2020
- Full Text
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49. A paper-based colorimetric sensor array for discrimination and simultaneous determination of organophosphate and carbamate pesticides in tap water, apple juice, and rice.
- Author
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Bordbar MM, Nguyen TA, Arduini F, and Bagheri H
- Subjects
- Carbamates chemistry, Colorimetry methods, Food Contamination, Fruit and Vegetable Juices analysis, Gold, Metal Nanoparticles, Organophosphates chemistry, Paper, Silver, Water chemistry, Water Pollutants, Chemical, Colorimetry instrumentation, Malus chemistry, Oryza chemistry, Pesticides chemistry
- Abstract
A colorimetric paper-based sensor is proposed for the rapid monitoring of six major organophosphate and carbamate pesticides. The assay was constructed by dropping gold and silver nanoparticles on the hydrophilic zones of a paper substrate. The nanoparticles were modified by L-arginine, quercetin, and polyglutamic acid. The mechanism of sensing is based on the interaction between the pesticide and the nanoparticles. The color of nanoparticles changed during the interactions. A digital camera recorded these changes. The assay provided a unique response for each studied pesticide. This method can determine six individual pesticides including carbaryl, paraoxon, parathion, malathion, diazinon, and chlorpyrifos. The limit of detection for these pesticides were 29.0, 22.0, 32.0, 17.0, 45.0, and 36.0 ng mL
-1 , respectively. The assay was applied to simultaneously determine the six studied pesticides in a mixture using the partial least square method (PLS). The root mean square errors of prediction were 11, 8.7, 9.2, 10, 12, and 11 for carbaryl, paraoxon, parathion, malathion, diazinon, and chlorpyrifos, respectively. The paper-based device can differentiate two types of studied pesticide (organophosphate and carbamate) as well as two types of organophosphate structures (oxon and thion). Furthermore, this sensor showed high selectivity to the pesticides in the presence of other potential species (e.g., metal ions, anions, amino acids, sugar, and vitamins). This assay is capable of determining the pesticide compounds in tap water, apple juice, and rice samples.Graphical abstract.- Published
- 2020
- Full Text
- View/download PDF
50. Novel bio-lab-on-a-tip for electrochemical glucose sensing in commercial beverages.
- Author
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Cinti S, Marrone R, Mazzaracchio V, Moscone D, and Arduini F
- Subjects
- Beverages analysis, Electrodes, Glucose, Glucose Oxidase, Biosensing Techniques, Electrochemical Techniques
- Abstract
The development of portable and user-friendly sensing platforms is a hot topic in the field of analytical chemistry. Among others, electroanalytical approaches exhibit a high amenability for reaching this purpose, i.e. the commercial strips for diabetes care are an obvious success. However, providing fully-integrated and reagent-free methods is always a leitmotiv. In this work, we evaluated the use of a disposable pipette tip, opportunely configured to demonstrate the first example of an electrochemical biosystem in a pipette tip, namely bio-lab-on-a-tip. The combination of a pipette tip, wire electrodes, enzyme, and cotton wool filter, allows the fabrication of a novel electroanalytical platform that does not need expertise-required tasks. To demonstrate the feasibility of this novel method, glucose is detected in beverages by means of chronoamperometry. The experimental setup, entirely built inside the pipette tip, is able to 1) block impurities/interferences from matrix, 2) load/release reagents for the bio-assay, 3) reduce the operating task to zero, and 4) perform electrochemical detection. With optimized experimental parameters, the bio-lab-on-a-tip is able to detect glucose linearly up to 10 mM with a detection limit of 170 μM. The effectiveness of the platform was confirmed by testing commercial beverages, e.g. Coca-Cola and Coca-Cola Zero, with high accuracy. In addition, the shelf-life of the novel device was evaluated, highlighting the role of cotton wool filter for providing a suitable environment for glucose oxidase stability. The novel concept can be easily generalized for further applications in the field of non-invasive clinical diagnostics and in-situ environmental monitoring., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
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