24 results on '"Aranda, Marcos L."'
Search Results
2. The Retinal Basis of Light Aversion in Neonatal Mice.
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Caval-Holme, Franklin S, Aranda, Marcos L, Chen, Andy Q, Tiriac, Alexandre, Zhang, Yizhen, Smith, Benjamin, Birnbaumer, Lutz, Schmidt, Tiffany M, and Feller, Marla B
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Neurosciences ,Pediatric ,Eye Disease and Disorders of Vision ,2.1 Biological and endogenous factors ,Aetiology ,Underpinning research ,1.1 Normal biological development and functioning ,Eye ,Animals ,Animals ,Newborn ,Gap Junctions ,Mice ,Retina ,Retinal Ganglion Cells ,Rod Opsins ,Vision ,Ocular ,connexin ,Cx45 ,Cx30.2 ,development ,enucleation ,photocurrent ,Medical and Health Sciences ,Psychology and Cognitive Sciences ,Neurology & Neurosurgery - Abstract
Aversive responses to bright light (photoaversion) require signaling from the eye to the brain. Melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) encode absolute light intensity and are thought to provide the light signals for photoaversion. Consistent with this, neonatal mice exhibit photoaversion before the developmental onset of image vision, and melanopsin deletion abolishes photoaversion in neonates. It is not well understood how the population of ipRGCs, which constitutes multiple physiologically distinct types (denoted M1-M6 in mouse), encodes light stimuli to produce an aversive response. Here, we provide several lines of evidence that M1 ipRGCs that lack the Brn3b transcription factor drive photoaversion in neonatal mice. First, neonatal mice lacking TRPC6 and TRPC7 ion channels failed to turn away from bright light, while two photon Ca2+ imaging of their acutely isolated retinas revealed reduced photosensitivity in M1 ipRGCs, but not other ipRGC types. Second, mice in which all ipRGC types except for Brn3b-negative M1 ipRGCs are ablated exhibited normal photoaversion. Third, pharmacological blockade or genetic knockout of gap junction channels expressed by ipRGCs, which reduces the light sensitivity of M2-M6 ipRGCs in the neonatal retina, had small effects on photoaversion only at the brightest light intensities. Finally, M1s were not strongly depolarized by spontaneous retinal waves, a robust source of activity in the developing retina that depolarizes all other ipRGC types. M1s therefore constitute a separate information channel between the neonatal retina and brain that could ensure behavioral responses to light but not spontaneous retinal waves.SIGNIFICANCE STATEMENT At an early stage of development, before the maturation of photoreceptor input to the retina, neonatal mice exhibit photoaversion. On exposure to bright light, they turn away and emit ultrasonic vocalizations, a cue to their parents to return them to the nest. Neonatal photoaversion is mediated by intrinsically photosensitive retinal ganglion cells (ipRGCs), a small percentage of the retinal ganglion cell population that express the photopigment melanopsin and depolarize directly in response to light. This study shows that photoaversion is mediated by a subset of ipRGCs, called M1-ipRGCs. Moreover, M1-ipRGCs have reduced responses to retinal waves, providing a mechanism by which the mouse distinguishes light stimulation from developmental patterns of spontaneous activity.
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- 2022
3. Melatonin Prevents Non-image-Forming Visual System Alterations Induced by Experimental Glaucoma in Rats
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González Fleitas, María F., Devouassoux, Julián, Aranda, Marcos L., Dieguez, Hernán H., Calanni, Juan S., Iaquinandi, Agustina, Sande, Pablo H., Dorfman, Damián, and Rosenstein, Ruth E.
- Published
- 2021
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4. Diversity of intrinsically photosensitive retinal ganglion cells: circuits and functions
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Aranda, Marcos L. and Schmidt, Tiffany M.
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- 2021
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5. Histological validation of per-bundle water diffusion metrics within a region of fiber crossing following axonal degeneration
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Rojas-Vite, Gilberto, Coronado-Leija, Ricardo, Narvaez-Delgado, Omar, Ramírez-Manzanares, Alonso, Marroquín, José Luis, Noguez-Imm, Ramsés, Aranda, Marcos L., Scherrer, Benoit, Larriva-Sahd, Jorge, and Concha, Luis
- Published
- 2019
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6. The “Use It or Lose It” Dogma in the Retina: Visual Stimulation Promotes Protection Against Retinal Ischemia
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Fleitas, María Florencia González, Aranda, Marcos L., Diéguez, Hernán H., Milne, Georgia, Langellotti, Leonel, Miranda, Magdalena, Altschuler, Florencia, Dorfman, Damian, and Rosenstein, Ruth E.
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- 2020
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7. Critical Role of Monocyte Recruitment in Optic Nerve Damage Induced by Experimental Optic Neuritis
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Aranda, Marcos L., Guerrieri, Diego, Piñero, Gonzalo, González Fleitas, María F., Altschuler, Florencia, Dieguez, Hernán H., Keller Sarmiento, María I., Chianelli, Mónica S., Sande, Pablo H., Dorfman, Damián, and Rosenstein, Ruth E.
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- 2019
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8. All the PNS is a Stage: Transplanted Bone Marrow Cells Play an Immunomodulatory Role in Peripheral Nerve Regeneration.
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Piñero, Gonzalo, Vence, Marianela, Aranda, Marcos L., Cercato, Magalí C., Soto, Paula A., Usach, Vanina, and Setton-Avruj, Patricia C.
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SCIATIC nerve injuries ,BONE marrow cells ,NERVOUS system regeneration ,PERIPHERAL nervous system ,PERIPHERAL nerve injuries ,NITRIC-oxide synthases ,MACROPHAGE inflammatory proteins ,IMMUNOLOGICAL adjuvants - Abstract
Working on a Wallerian degeneration model in rats, our group has shown the beneficial effects of systemic bone marrow mononuclear cell transplant on morphological and functional parameters, as well as the prevention of neuropathic pain. The current work thus seeks to evaluate the effect of systemic bone marrow cell transplant in a mouse model of sciatic nerve crush and aims to dig deeper into the mechanisms involved in bone marrow cell therapy. Adult C57BL/6J mice were subjected to 8s sciatic nerve crush and intravenously transplanted with bone marrow cells. Cells were tracked using a fluorescent probe, and the evolution of the degeneration–regeneration process was evaluated through axonal and myelin marker immunodetection at different survival times. Gene and protein expression of the main cytokines involved in the inflammatory phase and lesion-associated macrophage phenotypes were also analyzed. Initial findings corroborated the beneficial effect of bone marrow cells on the regenerative process and proved their efficiency in reducing the expression of some proinflammatory cytokines and increasing that of anti-inflammatory interleukin 10 (IL-10). In addition, transplanted animals showed a decrease in inducible nitric oxide synthase (iNOS)
+ macrophages, an increment in CD206+ cells, and an anticipated rise in Arg-1+ macrophages. Taken together, our results endorse bone marrow cell therapy as an alternative approach to accelerate nerve recovery and postulate bone marrow cells as potential immunomodulators. Summary Statement: Bone marrow cell transplant has proven to be an effective therapeutic approach to treat peripheral nervous system injuries as it not only promoted regeneration and remyelination of the injured nerve but also had a potent effect on neuropathic pain. [ABSTRACT FROM AUTHOR]- Published
- 2023
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9. Post-ischemic environmental enrichment protects the retina from ischemic damage in adult rats
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Dorfman, Damián, Fernandez, Diego C., Chianelli, Mónica, Miranda, Magdalena, Aranda, Marcos L., and Rosenstein, Ruth E.
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- 2013
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10. Neuroprotective effect of melatonin in experimental optic neuritis in rats
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Aranda, Marcos L., González Fleitas, María F., De Laurentiis, Andrea, Keller Sarmiento, María I., Chianelli, Mónica, Sande, Pablo H., Dorfman, Damián, and Rosenstein, Ruth E.
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- 2016
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11. Experimental optic neuritis induced by the microinjection of lipopolysaccharide into the optic nerve
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Aranda, Marcos L., Dorfman, Damián, Sande, Pablo H., and Rosenstein, Ruth E.
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- 2015
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12. Melatonin protects the retina from experimental nonexudative age‐related macular degeneration in mice.
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Diéguez, Hernán H., González Fleitas, María F., Aranda, Marcos L., Calanni, Juan S., Keller Sarmiento, María I., Chianelli, Mónica S., Alaimo, Agustina, Sande, Pablo H., Romeo, Horacio E., Rosenstein, Ruth E., and Dorfman, Damián
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RETINAL degeneration ,RETINA ,RHODOPSIN ,MELATONIN ,OXIDATIVE stress - Abstract
Nonexudative age‐related macular degeneration (NE‐AMD) represents the leading cause of blindness in the elderly. Currently, there are no available treatments for NE‐AMD. We have developed a NE‐AMD model induced by superior cervical ganglionectomy (SCGx) in C57BL/6J mice, which reproduces the disease hallmarks. Several lines of evidence strongly support the involvement of oxidative stress in NE‐AMD‐induced retinal pigment epithelium (RPE) and outer retina damage. Melatonin is a proven and safe antioxidant. Our aim was analysing the effect of melatonin in the RPE/outer retina damage within experimental NE‐AMD. The treatment with melatonin starting 48 h after SCGx, which had no effect on the ubiquitous choriocapillaris widening, protected visual functions and avoided Bruch´s membrane thickening, RPE melanin content, melanosome number loss, retinoid isomerohydrolase (RPE65)‐immunoreactivity decrease, and RPE and hotoreceptor ultrastructural damage induced within experimental NE‐AMD exclusively located at the central temporal (but not nasal) region. Melatonin also prevented the increase in outer retina/RPE oxidative stress markers and a decrease in mitochondrial mass at 6 weeks post‐SCGx. Moreover, when the treatment with melatonin started at 4 weeks post‐SCGx, it restored visual functions and reversed the decrease in RPE melanin content and RPE65‐immunoreactivity. These findings suggest that melatonin could become a promising safe therapeutic strategy for NE‐AMD. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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13. Enriched environment provides neuroprotection against experimental glaucoma.
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González Fleitas, María F., Devouassoux, Julián D., Aranda, Marcos L., Calanni, Juan S., Chianelli, Monica S., Dorfman, Damián, and Rosenstein, Ruth E.
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ANTERIOR chamber (Eye) ,OLIGODENDROGLIA ,RETINAL ganglion cells ,VISUAL evoked potentials ,GLAUCOMA ,BRAIN-derived neurotrophic factor ,OPTIC nerve - Abstract
Glaucoma is one of the most frequent causes of visual impairment worldwide, and involves selective damage to retinal ganglion cells (RGCs) and their axons. We analyzed the effect of enriched environment (EE) housing on the optic nerve, and retinal alterations in an induced model of ocular hypertension. For this purpose, male Wistar rats were weekly injected with vehicle or chondroitin sulfate (CS) into the eye anterior chamber for 10 weeks and housed in standard environment or EE. EE housing prevented the effect of experimental glaucoma on visual evoked potentials, retinal anterograde transport, phosphorylated neurofilament‐immunoreactivity, axon number, microglial/macrophage reactivity (ionized calcium binding adaptor molecule 1‐immunoreactivity), and astrocytosis (glial fibrillary acidic protein‐immunostaining), as well as oligodendrocytes alterations (luxol fast blue staining, and myelin basic protein‐immunoreactivity) in the proximal portion of the optic nerve. Moreover EE prevented the increase in ionized calcium binding adaptor molecule‐1 levels, and RGC loss (Brn3a‐immunoreactivity) in the retina from hypertensive eyes. EE increased retinal brain‐derived neurotrophic factor levels. When EE housing started after 6 weeks of ocular hypertension, a preservation of visual evoked potentials amplitude, axon, and Brn3a(+) RGC number was observed. Taken together, these results suggest that EE preserved visual functions, reduced optic nerve axoglial alterations, and protected RGCs against glaucomatous damage. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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14. Therapeutic benefit of environmental enrichment on optic neuritis.
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Aranda, Marcos L., González Fleitas, María F., Dieguez, Hernán H., Milne, Georgia A., Devouassoux, Julián D., Keller Sarmiento, María I., Chianelli, Mónica, Sande, Pablo H., Dorfman, Damián, and Rosenstein, Ruth E.
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OPTIC neuritis , *ENVIRONMENTAL enrichment , *NEURODEGENERATION , *MICROINJECTION (Cytology) , *VISUAL evoked potentials - Abstract
Abstract Optic neuritis (ON) is an inflammatory, demyelinating, neurodegenerative, and presently untreatable condition of the optic nerve which might induce blindness. We analyzed the effect of environmental enrichment (EE) on visual pathway damage provoked by experimental ON induced by a microinjection of bacterial lipopolysaccharide (LPS) into the optic nerve. For this purpose, LPS was microinjected into the optic nerve from male Wistar rats. After injection, one group of animals was submitted to EE, and another group remained in standard environment (SE) for 21 days. EE prevented the decrease in pupil light reflex (PLR), visual evoked potentials, retinal anterograde transport, phosphorylated neurofilament immunoreactivity, myelination (luxol fast blue staining), and axon (toluidine blue staining) and retinal ganglion cell (Brn3a-immunoreactivity) number. EE also prevented microglial/macrophage reactivity (Iba-1- and ED1-immunoreactivity), and astrocytosis (glial fibrillary acidic protein-immunostaining) induced by experimental ON. LPS-injected optic nerves displayed oxidative damage and increased inducible nitric oxide synthase, cyclooxygenase-2, and interleukin-1β and TNFα mRNA levels which were prevented by EE. EE increased optic nerve brain-derived neurotrophic factor levels. When EE started at 4 (but not 7) days post-injection of LPS, a preservation of the PLR was observed at 21 days post-LPS, which was blocked by the daily administration of ANA-12 from day 4 to day 7 post-LPS. Moreover, EE from day 4 to day 7 post-LPS significantly preserved the PLR at 21 days post-injection. Taken together, our data suggest that EE preserved visual functions and reduced neuroinflammation of the optic nerve. This article is part of the Special Issue entitled "Neurobiology of Environmental Enrichment". Highlights • Environmental enrichment (EE) protects visual functions against optic neuritis. • EE protects retinal anterograde transport and prevents microglial reactivity. • EE prevents astrocytosis, demyelination, and axon and retinal ganglion cell loss. • EE housing behaves as an anti-inflammatory, anti-oxidative, and pro-BDNF therapy. • A TrkB receptor antagonist blocks the protective effect of EE on visual function. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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15. Pre-ischemic enriched environment increases retinal resilience to acute ischemic damage in adult rats.
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González Fleitas, María F., Aranda, Marcos L., Dieguez, Hernán H., Devouassoux, Julian D., Chianelli, Mónica S., Dorfman, Damian, and Rosenstein, Ruth E.
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VISION disorders , *REPERFUSION injury , *CELL nuclei , *SUPERIOR colliculus , *BLINDNESS , *RETINAL ganglion cells , *EYE - Abstract
Abstract Retinal ischemia is a condition associated with several degenerative diseases leading to visual impairment and blindness worldwide. Currently, there is no highly effective therapy for ischemic retinopathies. This study was designed to determine possible benefits of pre-exposure to enriched environment against retinal damage induced by acute ischemia. For this purpose, adult male Wistar rats were randomly assigned to a pre-ischemic standard environment or a pre-ischemic enriched environment for 3 weeks, followed by unilateral ischemia induced by increasing intraocular pressure above 120 mm Hg for 40 min and reperfusion for 1 or 2 weeks in standard environment. Animals were subjected to electroretinography and histological analysis. Pre-ischemic enriched environment afforded significant functional protection in eyes exposed to ischemia/reperfusion injury. A marked reduction in retinal layer thickness, reduced synaptophysin-immunoreactivity and retinal ganglion cell (RGC) number, and increased microglia/macrophage reactivity were observed in ischemic retinas from animals submitted to pre-ischemic standard environment, which were prevented by pre-ischemic enriched environment. A deficit in anterograde transport from the retina to the superior colliculus and the lateral geniculate nucleus was observed in animals exposed to pre-ischemic standard environment, which was lower in animals previously exposed to enriched environment. The exposure to enriched environment before ischemia increased retinal brain derived neurotrophic factor (BDNF) protein levels in ischemic retinas and the administration of ANA-12 (a TrkB antagonist) abolished the protective effect of enriched environment on retinal function and retinal ganglion cell number. These results indicate that pre-ischemic enriched environment increases retinal resilience to acute ischemic damage, possibly through a BDNF/TrkB mediated pathway. Highlights • Enriched environment prior to ischemia protects retinal function against ischemia. • Enriched environment (EE) prevents structural alterations induced by ischemia. • EE prior to ischemia increases retinal BDNF levels. • A TrkB antagonist abolishes the protective effect of EE. • EE prior to ischemia increases retinal resilience to acute ischemic damage. [ABSTRACT FROM AUTHOR]
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- 2019
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16. Enriched Environment Protects the Optic Nerve from Early Diabetes-Induced Damage in Adult Rats.
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Dorfman, Damián, Aranda, Marcos L., and Rosenstein, Ruth E.
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OPTIC nerve , *DIABETES , *LABORATORY rats , *DIABETIC retinopathy , *ETIOLOGY of diseases , *STREPTOZOTOCIN - Abstract
Diabetic retinopathy is a leading cause of reduced visual acuity and acquired blindness. Axoglial alterations of the distal (close to the chiasm) optic nerve (ON) could be the first structural change of the visual pathway in streptozotocin (STZ)-induced diabetes in rats. We analyzed the effect of environmental enrichment on axoglial alterations of the ON provoked by experimental diabetes. For this purpose, three days after vehicle or STZ injection, animals were housed in enriched environment (EE) or remained in a standard environment (SE) for 6 weeks. Anterograde transport, retinal morphology, optic nerve axons (toluidine blue staining and phosphorylated neurofilament heavy immunoreactivity), microglia/macrophages (ionized calcium binding adaptor molecule 1 (Iba-1) immunoreactivity), astrocyte reactivity (glial fibrillary acid protein-immunostaining), myelin (myelin basic protein immunoreactivity), ultrastructure, and brain derived neurotrophic factor (BDNF) levels were assessed in non-diabetic and diabetic animals housed in SE or EE. No differences in retinal morphology or retinal ganglion cell number were observed among groups. EE housing which did not affect the STZ-induced weight loss and hyperglycemia, prevented a decrease in the anterograde transport from the retina to the superior colliculus, ON axon number, and phosphorylated neurofilament heavy immunoreactivity. Moreover, EE housing prevented an increase in Iba-1 immunoreactivity, and astrocyte reactivity, as well as ultrastructural myelin alterations in the ON distal portion at early stages of diabetes. In addition, EE housing avoided a decrease in BDNF levels induced by experimental diabetes. These results suggest that EE induced neuroprotection in the diabetic visual pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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17. Environmental Enrichment Protects the Retina from Early Diabetic Damage in Adult Rats.
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Dorfman, Damián, Aranda, Marcos L., González Fleitas, María Florencia, Chianelli, Mónica S., Fernandez, Diego C., Sande, Pablo H., and Rosenstein, Ruth E.
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DIABETIC retinopathy , *ENVIRONMENTAL enrichment , *VISUAL acuity , *BLINDNESS , *SYNAPTOPHYSIN , *RAT diseases , *RETINAL diseases - Abstract
Diabetic retinopathy is a leading cause of reduced visual acuity and acquired blindness. Available treatments are not completely effective. We analyzed the effect of environmental enrichment on retinal damage induced by experimental diabetes in adult Wistar rats. Diabetes was induced by an intraperitoneal injection of streptozotocin. Three days after vehicle or streptozotocin injection, animals were housed in enriched environment or remained in a standard environment. Retinal function (electroretinogram, and oscillatory potentials), retinal morphology, blood-retinal barrier integrity, synaptophysin, astrocyte and Müller cell glial fibrillary acidic protein, vascular endothelial growth factor, tumor necrosis factor-α, and brain-derived neurotrophic factor levels, as well as lipid peroxidation were assessed in retina from diabetic animals housed in standard or enriched environment. Environmental enrichment preserved scotopic electroretinogram a-wave, b-wave and oscillatory potential amplitude, avoided albumin-Evan's blue leakage, prevented the decrease in retinal synaptophysin and astrocyte glial fibrillary acidic protein levels, the increase in Müller cell glial fibrillary acidic protein, vascular endothelial growth factor and tumor necrosis factor-α levels, as well as oxidative stress induced by diabetes. In addition, enriched environment prevented the decrease in retinal brain-derived neurotrophic factor levels induced by experimental diabetes. When environmental enrichment started 7 weeks after diabetes onset, retinal function was significantly preserved. These results indicate that enriched environment could attenuate the early diabetic damage in the retina from adult rats. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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18. Chronobiotic effect of melatonin in experimental optic neuritis.
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Aranda, Marcos L., Narvaez, Omar, Altschuler, Florencia, Calanni, Juan S., González Fleitas, María F., Sande, Pablo H., Dorfman, Damián, Concha, Luis, and Rosenstein, Ruth E.
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OPTIC neuritis , *SUPRACHIASMATIC nucleus , *NEURITIS , *RETINAL ganglion cells , *MELATONIN , *BLUE light , *OPTIC nerve - Abstract
Optic neuritis (ON) is an inflammatory condition of the optic nerve, which leads to retinal ganglion cell (RGC) loss. A subset of RGCs expressing the photopigment melanopsin regulates non-image-forming visual system (NIFVS) functions such as pupillary light reflex (PLR) and circadian rhythms. Melatonin is a chronobiotic agent able to regulate the circadian system. We analyzed the effect of ON on the NIFVS, and the effect of melatonin on the NIFVS alterations induced by ON. For this purpose, optic nerves from male Wistar rats received vehicle or bacterial lipopolysaccharide (LPS), and one group of animals received a subcutaneous pellet of melatonin or a sham procedure. The NIFVS was analyzed in terms of: i) blue light-evoked PLR, ii) the communication between the retina and the suprachiasmatic nuclei (by anterograde transport, and ex vivo magnetic resonance images), iii) locomotor activity rhythm, and iv) Brn3a(+) and melanopsin(+) RGC number (by immunohistochemistry). Experimental ON significantly decreased the blue light-evoked PLR, induced a misconnection between the retina and the suprachiasmatic nuclei, decreased Brn3a(+) RGCs, but not melanopsin(+) RGC number. A bilateral injection of LPS significantly increased the light (but not dark) phase locomotor activity, rhythm periodicity, and time of offset activity. Melatonin prevented the decrease in blue light-evoked PLR, and locomotor activity rhythm alterations induced by ON. These results support that ON provoked alterations of the circadian physiology, and that melatonin could restore the circadian system misalignment. • Optic neuritis leads to retinal ganglion cell loss and visual dysfunction. • Melatonin is a chronobiotic agent able to regulate the circadian system. • Optic neuritis alters the pupillary reflex and locomotor activity rhythm. • Melatonin prevents optic neuritis-induced circadian alterations. • Melatonin could restore the circadian system misalignment induced by optic neuritis. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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19. Oxidative stress damage circumscribed to the central temporal retinal pigment epithelium in early experimental non-exudative age-related macular degeneration.
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Dieguez, Hernán H., Romeo, Horacio E., Alaimo, Agustina, González Fleitas, María F., Aranda, Marcos L., Rosenstein, Ruth E., and Dorfman, Damián
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RETINAL degeneration , *OXIDATIVE stress , *MITOCHONDRIA , *LIPID peroxidation (Biology) , *HEME oxygenase - Abstract
Abstract Non-exudative age-related macular degeneration (NE-AMD) represents the leading cause of blindness in the elderly. The macular retinal pigment epithelium (RPE) lies in a high oxidative environment because its high metabolic demand, mitochondria concentration, reactive oxygen species levels, and macular blood flow. It has been suggested that oxidative stress-induced damage to the RPE plays a key role in NE-AMD pathogenesis. The fact that the disease limits to the macular region raises the question as to why this area is particularly susceptible. We have developed a NE-AMD model induced by superior cervical ganglionectomy (SCGx) in C57BL/6J mice, which reproduces the disease hallmarks exclusively circumscribed to the temporal region of the RPE/outer retina. The aim of this work was analyzing RPE regional differences that could explain AMD localized susceptibility. Lower melanin content, thicker basal infoldings, higher mitochondrial mass, and higher levels of antioxidant enzymes, were found in the temporal RPE compared with the nasal region. Moreover, SCGx induced a decrease in the antioxidant system, and in mitochondria mass, as well as an increase in mitochondria superoxide, lipid peroxidation products, nuclear Nrf2 and heme oxygenase-1 levels, and in the occurrence of damaged mitochondria exclusively at the temporal RPE. These findings suggest that despite the well-known differences between the human and mouse retina, it might not be NE-AMD pathophysiology which conditions the localization of the disease, but the macular RPE histologic and metabolic specific attributes that make it more susceptible to choroid alterations leading initially to a localized RPE dysfunction/damage, and secondarily to macular degeneration. Graphical abstract fx1 Highlights • The temporal retinal pigment epithelium (tRPE) has lower melanin content. • Higher antioxidant enzyme levels and mitochondria mass occur in the tRPE. • Superior cervical ganglionectomy (SCGx) induces oxidative stress only in the tRPE. • SCGx decreases mitochondria mass circumscribed to the tRPE. • Oxidative stress and mitochondria are key factors in early macular degeneration. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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20. Genetic tuning of intrinsically photosensitive retinal ganglion cell subtype identity to drive visual behavior.
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Aranda ML, Bhoi JD, Parra OAP, Lee SK, Yamada T, Yang Y, and Schmidt TM
- Abstract
The melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) comprise a subset of the ∼40 retinal ganglion cell types in the mouse retina and drive a diverse array of light-evoked behaviors from circadian photoentrainment to pupil constriction to contrast sensitivity for visual perception. Central to the ability of ipRGCs to control this diverse array of behaviors is the distinct complement of morphophysiological features and gene expression patterns found in the M1-M6 ipRGC subtypes. However, the genetic regulatory programs that give rise to subtypes of ipRGCs are unknown. Here, we identify the transcription factor Brn3b (Pou4f2) as a key genetic regulator that shapes the unique functions of ipRGC subtypes and their diverse downstream visual behaviors.
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- 2024
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21. An ethologically relevant paradigm to assess visual contrast sensitivity in rodents.
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Calanni JS, Aranda ML, Dieguez HH, Dorfman D, Schmidt TM, and Rosenstein RE
- Abstract
In the animal kingdom, threat information is perceived mainly through vision. The subcortical visual pathway plays a critical role in the rapid processing of visual information-induced fear, and triggers a response. Looming-evoked behavior in rodents, mimicking response to aerial predators, allowed identify the neural circuitry underlying instinctive defensive behaviors; however, the influence of disk/background contrast on the looming-induced behavioral response has not been examined, either in rats or mice. We studied the influence of the dark disk/gray background contrast in the type of rat and mouse defensive behavior in the looming arena, and we showed that rat and mouse response as a function of disk/background contrast adjusted to a sigmoid-like relationship. Both sex and age biased the contrast-dependent response, which was dampened in rats submitted to retinal unilateral or bilateral ischemia. Moreover, using genetically manipulated mice, we showed that the three type of photoresponsive retinal cells (i.e., cones, rods, and intrinsically photoresponsive retinal ganglion cells (ipRGCs)), participate in the contrast-dependent response, following this hierarchy: cones ˃> rods ˃>>ipRGCs. The cone and rod involvement was confirmed using a mouse model of unilateral non-exudative age-related macular degeneration, which only damages canonical photoreceptors and significantly decreased the contrast sensitivity in the looming arena.
- Published
- 2024
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22. Histological and diffusion-weighted magnetic resonance imaging data from normal and degenerated optic nerve and chiasm of the rat.
- Author
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Narvaez-Delgado O, Rojas-Vite G, Coronado-Leija R, Ramírez-Manzanares A, Marroquín JL, Noguez-Imm R, Aranda ML, Scherrer B, Larriva-Sahd J, and Concha L
- Abstract
Diffusion-weighted magnetic resonance imaging (dMRI) is widely used to infer microstructural characteristics of tissue, particularly in cerebral white matter. Histological validation of the metrics derived from dMRI methods are needed to fully characterize their ability to capture biologically-relevant histological features non-invasively. The data described here were used to correlate metrics derived from dMRI and quantitative histology in an animal model of axonal degeneration ("Histological validation of per-bundle water diffusion metrics within a region of fiber crossing following axonal degeneration" [1]). Unilateral retinal ischemia/reperfusion was induced in 10 rats, by the elevation of pressure of the anterior chamber of the eye for 90 min. Five rats were used as controls. After five weeks, injured animals were intracardially perfused to analyze the optic nerves and chiasm with dMRI and histology. This resulted in 15 brain scans, each with 80 diffusion-sensitizing gradient directions with b = 2000 and 2500 s/mm
2 and 20 non-diffusion-weighted images (b = 0 s/mm2 ), with isometric voxel resolution of 125 μm3 . Histological sections were obtained after dMRI. Optical microscopy photomicrographs of the optic nerves (stained with toluidine blue) are available, as well as their corresponding automatic segmentations of axons and myelin.- Published
- 2019
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23. Superior cervical gangliectomy induces non-exudative age-related macular degeneration in mice.
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Dieguez HH, Romeo HE, González Fleitas MF, Aranda ML, Milne GA, Rosenstein RE, and Dorfman D
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- Animals, Bruch Membrane pathology, Bruch Membrane ultrastructure, Choroid pathology, Macular Degeneration pathology, Male, Melanins metabolism, Mice, Inbred C57BL, Photoreceptor Cells, Vertebrate metabolism, Retinal Pigment Epithelium metabolism, Superior Cervical Ganglion pathology, cis-trans-Isomerases metabolism, Macular Degeneration etiology, Superior Cervical Ganglion surgery
- Abstract
Non-exudative age-related macular degeneration, a prevalent cause of blindness, is a progressive and degenerative disease characterized by alterations in Bruch's membrane, retinal pigment epithelium, and photoreceptors exclusively localized in the macula. Although experimental murine models exist, the vast majority take a long time to develop retinal alterations and, in general, these alterations are ubiquitous, with many resulting from non-eye-specific genetic manipulations; additionally, most do not always reproduce the hallmarks of human age-related macular degeneration. Choroid vessels receive sympathetic innervation from the superior cervical ganglion, which, together with the parasympathetic system, regulates blood flow into the choroid. Choroid blood flow changes have been involved in age-related macular degeneration development and progression. At present, no experimental models take this factor into account. The aim of this work was to analyze the effect of superior cervical gangliectomy (also known as ganglionectomy) on the choroid, Bruch's membrane, retinal pigment epithelium and retina. Adult male C57BL/6J mice underwent unilateral superior cervical gangliectomy and a contralateral sham procedure. Although superior cervical gangliectomy induced ubiquitous choroid and choriocapillaris changes, it induced Bruch's membrane thickening, loss of retinal pigment epithelium melanin content and retinoid isomerohydrolase, the appearance of drusen-like deposits, and retinal pigment epithelium and photoreceptor atrophy, exclusively localized in the temporal side. Moreover, superior cervical gangliectomy provoked a localized increase in retinal pigment epithelium and photoreceptor apoptosis, and a decline in photoreceptor electroretinographic function. Therefore, superior cervical gangliectomy recapitulated the main features of human non-exudative age-related macular degeneration, and could become a new experimental model of dry age-related macular degeneration, and a useful platform for developing new therapies., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2018. Published by The Company of Biologists Ltd.)
- Published
- 2018
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24. Melatonin as a Therapeutic Resource for Inflammatory Visual Diseases.
- Author
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Aranda ML, Fleitas MFG, Dieguez H, Iaquinandi A, Sande PH, Dorfman D, and Rosenstein RE
- Subjects
- Animals, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Humans, Melatonin pharmacology, Anti-Inflammatory Agents, Non-Steroidal therapeutic use, Melatonin therapeutic use, Optic Neuritis drug therapy, Uveitis drug therapy
- Abstract
Background: Uveitis and optic neuritis are prevalent ocular inflammatory diseases, and highly damaging ocular conditions. Both diseases are currently treated with corticosteroids, but they do not have adequate efficacy and are often associated with severe side effects. Thus, uveitis and optic neuritis remain a challenging field to ophthalmologists and a significant public health concern., Objective: This review summarizes findings showing the benefits of a treatment with melatonin in experimental models of these inflammatory ocular diseases., Results: Oxidative and nitrosative damage, tumor necrosis factor, and prostaglandin production have been involved in the pathogeny of uveitis and optic neuritis. Melatonin is an efficient antioxidant and antinitridergic, and has the ability to reduce prostaglandin and tumor necrosis factor levels both in the retina and optic nerve. Moreover, melatonin not only prevents functional and structural consequences of experimental uveitis and optic neuritis, but it is also capable of suppressing the actively ongoing ocular inflammatory response., Conclusions: Since melatonin protects ocular tissues against inflammation, it could be a potentially useful anti-inflammatory therapy in ophthalmology., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
- Published
- 2017
- Full Text
- View/download PDF
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