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The enormous industrial usage of nickel during its manufacture and recycling has led to widespread environmental pollution. This study was designed to examine the ability of Gelidium amansii biomass to biosorb Ni2+ ions from an aqueous solution. Six independent variables, including contact time (1.0 and 3.0 h), pH (4 and 7), Ni2+ concentration (25 and 200 mg·L−1), temperature (25°C and 50°C), G. amansii biomass (1.0 and 4.0 g·L−1), and agitation mode (agitation or static), were investigated to detect the significance of each factor using a Plackett–Burman design. The analysis of variance for the Ni2+ biosorption percentage indicated that three independent variables (contact time, temperature, and agitation–static mode) exhibited a high level of significance in the Ni2+ biosorption process. Twenty experiments were conducted containing six axial, eight factorial, and six replicates points at center points. The resulting face-centered central composite design analysis data for the biosorption of Ni2+ exhibited a very large variation in the removal percentage of Ni2+, which ranged from 29.73 to 100.00%. The maximum Ni2+ biosorption percentage was achieved in the 16th run with an experimental percentage quantified as 100.00% under the experimental conditions of 3 h of incubation time and 45°C with 100 rpm for agitation speed.

Developers are encouraged to adopt good design practices to maintain good software quality during the system's evolution. However, some modifications and changes to the system could cause code smells and pattern grime, which might incur more maintenance effort. As the presence of both code smells and pattern grime is considered a bad sign and raises a flag at code segments that need more careful examination, a potential connection between them may exist. Therefore, the main objective of this paper is to (1) empirically investigate the potential relationship between the accumulation of pattern grime and the presence of code smells and (2) evaluate the significance of individual code smells when they appear in a specific pattern grime category. To achieve this goal, we performed an empirical study using six‐grime metrics and 10 code smells on five Java open‐source projects ranging from 217 to 563 classes. Our statistical results indicate that, in general, the growth of grime is more likely to co‐occur with code smells using Spearman's correlation and Odd Ratio test. Specifically, there is a strong positive association between the growth of pattern grime at the class level and the presence of Shotgun Surgery smell according to the result of applying the Apriori algorithm, which gives conviction values equal to 1.66. The findings in this paper are helpful for developers and researchers as the presence of pattern grime could be considered a factor in improving the performance of existing smell detection methods. Furthermore, the link between grime and smells can be exploited as a hint for smell distribution in the system. [ABSTRACT FROM AUTHOR]

Bio-fertilizers are the most important and effective method used to reduce the quantities of chemical fertilizers consumed and reduce dependence on them in agricultural production to avoid their harmful effects on the environment and public health as well as reduce the cost of agricultural production in light of increasing pollution and under adverse conditions for production and climate change. A bio-fertilizer depends primarily on the use of beneficial microorganisms such as arbuscular mycorrhizal fungi (AMF) to improve the uptake of nutrients, improve plant growth, productivity, and grain yield. Crop production faces many challenges, and drought is one of the majority of the significant factors limiting crop production worldwide, especially in semi-arid regions. The objective of this study was to evaluate the effects of AMF and phosphate solubilizing bacteria (PSB), plus three rates of the recommended dose of phosphorus (RDP) fertilizer on yield, yield components, and nutrients uptake, in addition to evaluating the beneficial effects of these combinations to develop Phosphorus (P) management under three levels of irrigation water, i.e., three irrigations (normal or well-watered), two irrigations (moderate drought), and one irrigation (severe drought) on barley (Hordeum vulgare L.). The results showed that the treatment with AMF bio-fertilizer yielded the highest values of plant height, spike length, spike weight, number of grains/spike, 1000-grain weight, grain yield, straw yield, biological yield, and harvest index. Moreover, the grain and straw uptake of nitrogen (N), P, and potassium (K) (kg ha−1) in the two seasons under the three levels of irrigation, respectively, were superior followed by the inoculation by PSB. While the treatment without bio-fertilizer yielded the lowest values of these traits of barley, the treatment with bio-fertilizer yielded the increased percentage of the grain yield by 17.27%, 17.33% with applying AMF, and 10.31%, 10.40% with treatment by PSB. Treatment with AMF or PSB (Phosphorien), plus rates of phosphorus fertilizer under conditions of irrigation water shortage, whether irrigation was performed once or twice, led to an increase in grain yield and other characteristics compared to the same fertilization rates without inoculation. The results of this study showed that the use of bio-fertilizers led to an increase in plant tolerance to drought stress, and this was demonstrated by an increase in various traits with the use of treatments that include bio-fertilizers. Therefore, it is suggested to inoculate the seeds with AMF or PSB plus adding phosphate fertilizers at the recommended dose under drought conditions. [ABSTRACT FROM AUTHOR]

The water-retaining and yield-increasing capacity of super-absorbent polymer (SAP) are essential for soil remediation in arid and semi-arid areas. Water availability is an increasing challenge to plant development and crop yield. During the growing seasons in 2021 and 2022, the present study was conducted to evaluate the effect of the addition of different amounts of SAP on the development and yield of green beans (Phaseolus vulgaris L. cv Bronco) under varying water deficit stresses, compared with the control treatment without SAP and water deficit stress. The results demonstrated that a 50% reduction in water requirement (WR) resulted in significant decreases in leaf fresh weight, specific leaf area, leaf total chlorophyll content, pod number, leaf free water content, pod fresh weight per plant, and yield. Decreases were also found in pod total chlorophyll content, carotenoids, dry matter and total protein, leaf proline content, and crude fiber content. Additionally, leaf water saturation deficit was significantly increased under the stress compared with the full irrigation at 100% WR. However, irrigation at 75% WR increased pod contents of ascorbic acid, total sugars, and leaf bound water. The current study also indicated that addition of SAP significantly enhanced the above-mentioned growth characteristics under irrigation at 50% and 75% WR. Treatment with SAP at 3 g/plant was the most effective in mitigating the adverse effects of water deficiency, especially at the irrigation rate of 75% WR. Pearson's correlation analysis showed significantly positive correlations between the growth parameters, as well as pod yield, under water stress and SAP. This study provides a promising strategy for green bean cultivation by adding SAP to soil to alleviate water shortage stress. [ABSTRACT FROM AUTHOR]

Pseudomonas aeruginosa, a Gram-negative bacterium, is recognized for its adaptability and opportunistic nature. It poses a substantial challenge in clinical settings due to its complicated antibiotic resistance mechanisms, biofilm formation, and capacity for persistent infections in both animal and human hosts. Recent studies revealed a potential zoonotic transmission of P. aeruginosa between animals, the environment, and human populations which highlights awareness of this microbe. Implementation of the One Health approach, which underscores the connection between human, animal, and environmental health, we aim to offer a comprehensive perspective on the current landscape of P. aeruginosa management. This review presents innovative strategies designed to counteract P. aeruginosa infections. Traditional antibiotics, while effective in many cases, are increasingly compromised by the development of multidrugresistant strains. Non-antibiotic avenues, such as quorum sensing inhibition, phage therapy, and nanoparticle-based treatments, are emerging as promising alternatives. However, their clinical application encounters obstacles like cost, side effects, and safety concerns. Effectively addressing P. aeruginosa infections necessitates persistent research efforts, advancements in clinical development, and a comprehension of host-pathogen interactions to deal with this resilient pathogen. [ABSTRACT FROM AUTHOR]

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Antimicrobial resistance is one of the largest medical challenges because of the rising frequency of opportunistic human microbial infections across the globe. This study aimed to extract chitosan from the exoskeletons of dead bees and load it with bee venom (commercially available as Apitoxin [Api]). Then, the ionotropic gelation method would be used to form nanoparticles that could be a novel drug-delivery system that might eradicate eight common human pathogens (i.e., two fungal and six bacteria strains). It might also be used to treat the human colon cancer cell line (Caco2 ATCC ATP-37) and human liver cancer cell line (HepG2ATCC HB-8065) cancer cell lines. The x-ray diffraction (XRD), Fourier transform infrared (FTIR), and dynamic light scattering (DLS) properties, &#950-potentials, and surface appearances of the nanoparticles were evaluated by transmission electron microscopy (TEM). FTIR and XRD validated that the Api was successfully encapsulated in the chitosan nanoparticles (ChB NPs). According to the TEM, the ChB NPs and the ChB NPs loaded with Apitoxin (Api@ChB NPs) had a spherical shape and uniform size distribution, with non-aggregation, for an average size of approximately 182 and 274 ± 3.8 nm, respectively, and their Zeta potential values were 37.8 ± 1.2 mV and - 10.9 mV, respectively. The Api@ ChB NPs had the greatest inhibitory effect against all tested strains compared with the ChB NPs and Api alone. The minimum inhibitory concentrations (MICs) of the Api, ChB NPs, and Api@ChB NPs were evaluated against the offer mentioned colony forming units (CFU/mL), and their lowest MIC values were 30, 25, and 12.5 µg mL-1, respectively, against Enterococcus faecalis. Identifiable morphological features of apoptosis were observed by 3 T3 Phototox software after Api@ChB NPs had been used to treat the normal Vero ATCC CCL-81, Caco2 ATCC ATP-37, and HepG2 ATCC HB-8065 cancer cell lines for 24 h. The morphological changes were clear in a concentration-dependent manner, and the ability of the cells was 250 to 500 µg mL-1. These results revealed that Api@ChB NPs may be a promising natural nanotreatment for common human pathogens. [ABSTRACT FROM AUTHOR]

This study targeted developing a novel Zinc oxide with alpha hematite nanoflowers (NFs)-loaded bee venom (Bv) (Bv-ZnO@αFe2O3 NFs) as a bio-natural product from bees to combine both the advantages of combination magnetic properties and the antimicrobial and anti-biofilm properties on isolated coliform bacteria from the effluent of wastewater treatment plants. About 24 isolates of treated wastewater isolates were multidrug resistant (MDR). The phylogenetic grouping of Escherichia coli (E. coli) and Klebsiella pneumonia (K. pneumonia) showed that the largest group was Group A, followed by Group B2 and Group B1. Fourier transform infrared (FTIR), The X-ray diffraction (XRD), and scanning electron microscopy-energy dispersive X-ray analysis (SEM− EDX) validated the coating operation's contact with Bv onto ZnO@αFe2O3 NFs. According to high-resolution transmission electron microscopy (TEM) and selected area electron diffraction (SAED), pattern analyses for prepared nanoformulations exhibited a spherical shape of αFe2O3 (~9–15 nm), and floral needle shapes with uniform distribution of size with aggregation of ZnOαFe2O3 and Bv-ZnO@αFe2O3 NFs around (~100–200 nm). The toxicity of Bv-ZnO@αFe2O3 NFs was comparable up to 125 µg mL−1, when it reached 64.79% (IC50, 107.18 µg mL−1). The antibacterial activity showed different zones of inhibition against different isolates. The biofilm inhibitory activity of NPs and NFs showed a highly significant reduction (p < 0.001) in treated biofilms with ZnO@αFe2O3 and Bv-ZnO@αFe2O3. In essence, ZnO@αFe2O3 and Bv-ZnO@αFe2O3 NFs are promising antimicrobials for inhibiting the growth and biofilm of MDR E. coli and K. pneumonia isolates, thereby, biocontrol of wastewater. [ABSTRACT FROM AUTHOR]

Background: The care of a child receiving chemotherapy has a significant impact on the parents and family structure, which has been explored in depth in a few studies in Saudi Arabia. The purpose of this study is to further explore the lived experiences of Saudi Arabian parents in caring for their children receiving chemotherapy. Methodology: A qualitative descriptive design was used in this study with a sample of 17 parents of children receiving chemotherapy. The participants were recruited using the snow-balling technique throughout the community, as well as from the University Hospital, Pediatric Oncology Department. The data were collected via in-person and virtual methods using semi-structured, open-ended interviews and analyzed using thematic analysis. Results: The findings of the study were placed into two main themes: family experience and stages of disease. Family experiences include the parents and siblings' experiences. The stages of disease include three sub-themes: the pre-diagnosis phase, diagnosis phase, and treatment phase. Conclusion: The cancer journey had a huge impact on the parents and siblings. The study revealed important findings related to the experiences during the stages of diseases and lived experiences of parents and siblings including psychological, physiological, social, and financial impacts. [ABSTRACT FROM AUTHOR]

In this study, a new modified chitosan conjugate (Chito-TZ) was developed via amide coupling between the acid chloride derivative of the methylthio-thidiazole compound and the free primary amino groups of chitosan. The product was characterized using several instrumental investigations, including Fourier-transform infrared spectroscopy (FT-IR), 1H-Nuclear magnetic resonance, X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), and X-ray diffraction (XRD). XRD indicated that the crystalline pattern of chitosan was interrupted after chemical modification with the thiadiazole derivative. Broido's model was used to determine the thermal activation energy Ea, and the results showed that the Ea for the first decomposition region of Chito-TZ is 24.70 KJ mol−1 lower than that required for chitosan (95.57 KJ mol−1), indicating the accelerating effect of the thiadiazole derivative on the thermal decomposition of Chito-TZ. The modified chitosan showed better antibacterial and antifungal activities than the non-modified chitosan; except for seed germination, chitosan was better. The Chito-TZ showed a low MIC value (25–50 µg mL−1) compared to Chito (50–100 µg mL−1). Moreover, the maximum inhibition percentages for plant-pathogenic fungi, Aspergillus niger, Fusarium oxysporum, and Fusarium solani, were attained at a concentration of 300 µg mL−1 with values of 35.4 ± 0.9–39.4 ± 1.7% for Chito and 45.2 ± 1.6–52.1 ± 1.3% for Chito-TZ. The highest germination percentages (%) of broad bean, shoot and root length and weight, and seed vigor index were obtained after Chito treatment with a concentration of 200 µg mL−1 compared to Chito-TZ. [ABSTRACT FROM AUTHOR]

Purpose: Salinity is a main factor in decreasing seed germination, plant growth and yield. Salinity stress is a major problem for economic crops, as it can reduce crop yields and quality. Salinity stress occurs when the soil or water in which a crop is grown has a high salt content. Biochar improve plant growth and physiological traits under salt stress. The aim of the present study, the impact of biochar on growth, root morphological traits and physiological properties of alfalfa, amaranth and maize and soil enzyme activities under saline sands. Methods: We studied the impact of biochar on plant growth and the physiological properties of alfalfa, amaranth and maize under salt stress conditions. After 40 days, plant growth parameters (plant height, shoot and root fresh weights), root morphological traits and physiological properties were measured. Soil nutrients such as the P, K and total N contents in soil and soil enzyme activities were analyzed. Results: The results showed that the maize, alfalfa, and amaranth under biochar treatments significantly enhanced the plant height and root morphological traits over the control. The biochar on significantly increased the total root length, root diameter, and root volume. Compared to the control, the biochar significantly increased the chlorophyll a and b content, total chlorophyll and carotenoid content under salt stress. Furthermore, the biochar significantly increased enzyme activities of soil under salt stress in the three crops. Conclusions: Biochar treatments promote plant growth and physiological traits of alfalfa, amaranth, and maize under the salt stress condition. Overall, biochar is an effective way to mitigate salinity stress in crops. It can help to reduce the amount of salt in the soil, improve the soil structure, and increase the availability of essential nutrients, which can all help to improve crop yields. [ABSTRACT FROM AUTHOR]

Objective: Rare disease Background: Primary tracheal schwannoma is a rare neurogenic tumor. Early stage presents with nonspecific symptoms, and asthma is sometimes misdiagnosed. However, as the tumor grows, it presents with obstructive symptoms of the tracheal lumen. This tumor has been managed by open resection surgery until recently, when endoscopic excision became an option. The endoscopic excision reduces complications, operative time, and postoperative recovery period and is indicated in nonrecurrent surgical cases in which tumors are up to 2 cm in size, are pedunculated, and have no extratracheal extension, or in cases of poor cardiopulmonary status. We present a rare case of primary tracheal schwannoma managed by endoscopic excision. Case Report: A 37-year-old man was referred to our clinic with progressive shortness of breath and wheezing that started 3 months prior to presentation. Computed tomography showed a well-defined rounded, solid intraluminal tracheal mass at the proximal segment (at the level of the thoracic inlet). There was no extratracheal extension or enlarged cervical lymph nodes. The patient underwent endoscopic excision of the mass. A sickle knife, micro scissor, and suction diathermy were used for incision, stripping, and hemostasis done through the tumor pedicle. The first postoperative visit after 2 weeks showed subjective symptom improvement, and the flexible bronchoscope showed a completely healed surgical site with patent airway. Histopathological examination and immunohistochemistry confirmed the diagnosis of primary tracheal schwannoma. Conclusions: Primary tracheal schwannoma is rare. An endoscopic excision is an excellent option, but patients need to be appropriately selected and followed up to avoid recurrence. [ABSTRACT FROM AUTHOR]

Herein, twelve fungal strains were isolated from a deteriorated historical manuscript dated back to the 18th century. The obtained fungal strains were identified, using the traditional method and ITS sequence analysis, as Cladosporium herbarum (two strains), Aspergillus fumigatus (five strains), A. ustus (one strain), A. flavus (two strains), A. niger (one strain), and Penicillium chrysogenum (one strain). The ability of these fungal strains to degrade the main components of the paper was investigated by their activity to secrete extracellular enzymes including cellulase, amylase, gelatinase, and pectinase. The cell-free filtrate (CFF) ability of the probiotic bacterial strain Lactobacillus rhamnosus ATCC-7469 to inhibit fungal growth was investigated. The metabolic profile of CFF was detected by GC-MS analysis, which confirmed the low and high molecular weight of various active chemical compounds. The safe dose to be used for the biocontrol of fungal growth was selected by investigating the biocompatibility of CFF and two normal cell lines, Wi38 (normal lung tissue) and HFB4 (normal human skin melanocyte). Data showed that the CFF has a cytotoxic effect against the two normal cell lines at high concentrations, with IC50 values of 525.2 ± 9.8 and 329.1 ± 4.2 µg mL−1 for Wi38 and HFB4, respectively. The antifungal activity showed that the CFF has promising activity against all fungal strains in a concentration-dependent manner. The highest antifungal activity (100%) was recorded for a concentration of 300 µg mL−1 with a zone of inhibition (ZOI) in the ranges of 21.3 ± 0.6 to 17.7 ± 0.5 mm. At a concentration of 100 µg mL−1, the activity of CFF remained effective against all fungal strains (100%), but its effectiveness decreased to only inhibit the growth of eight strains (66%) out of the total at 50 µg mL−1. In general, probiotic bacterial strains containing CFF are safe and can be considered as a potential option for inhibiting the growth of various fungal strains. It is recommended that they be used in the preservation of degraded historical papers. [ABSTRACT FROM AUTHOR]

Little is known about the obstacles medical students face when conducting research in Saudi Arabia. Moreover, the proportion of medical students in research has been unknown in our region compared to other regions. We sought to identify the barriers and motivators that influence undergraduate medical students in pursuing research. This was a cross-sectional study design, utilizing an online survey distributed through social media platforms from the 17th of December 2021 to the 8th of April 2022. The survey was distributed to four universities in Saudi Arabia. Participants' characteristics, details regarding involvement in research, and attitude towards research were collected. Frequency measures were used to characterize the demographics and chi-squared tests to determine associations. A total of 435 students were included in the final analysis. The highest proportion of students that responded were second year, followed by first year medical students. Less than half (47.6%) of medical students were involved in research. A significant correlation was revealed between the involvement in research and higher participants' Grade Point Average (GPA). The top three incentives for pursuing undergraduate research were "admission into residency programs" (44.8%), "interest in research" (28.7%), and "financial return" (10.8%). However, the top three limitations were "lack of time" (29.2%), "lack of mentoring" (16.8%), and "lack of interest in research" (14.7%). System-related barriers and motivators were the main reasons behind the involvement of medical students in research. Our study is a call for action to raise awareness among medical students about the importance of research and to provide solutions to overcome these barriers. [ABSTRACT FROM AUTHOR]

The peel aqueous extract of Punica granatum was utilized to fabricate zinc oxide nanoparticles (ZnO-NPs) as a green approach. The synthesized NPs were characterized by UV-Vis spectroscopy, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy, which was attached to an energy dispersive X-ray (SEM-EDX). Spherical, well arranged, and crystallographic structures of ZnO-NPs were formed with sizes of 10–45 nm. The biological activities of ZnO-NPs, including antimicrobial and catalytic activity for methylene blue dye, were assessed. Data analysis showed that the antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria, as well as unicellular fungi, was observed to occur in a dose-dependent manner, displaying varied inhibition zones and low minimum inhibitory concentration (MIC) values in the ranges of 6.25–12.5 µg mL–1. The degradation efficacy of methylene blue (MB) using ZnO-NPs is dependent on nano-catalyst concentration, contact time, and incubation condition (UV-light emission). The maximum MB degradation percentages of 93.4 ± 0.2% was attained at 20 µg mL−1 after 210 min in presence of UV-light. Data analysis showed that there is no significant difference between the degradation percentages after 210, 1440, and 1800 min. Moreover, the nano-catalyst showed high stability and efficacy to degrade MB for five cycles with decreasing values of 4%. Overall, P. granatum-based ZnO-NPs are promising tools to inhibit the growth of pathogenic microbes and degradation of MB in the presence of UV-light emission. [ABSTRACT FROM AUTHOR]

Introduction: The approval of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines and obtaining herd immunity raise the optimism about seeing the end of this pandemic but vaccine hesitancy or refusal to vaccinate is a major threat to progress toward achieving herd immunity. In this study, we identify baseline knowledge, myths, misconceptions, attitudes, beliefs, and behaviors toward the COVID-19 vaccine. This help to develop new strategies to raise awareness, correct misconceptions and improve acceptance of the COVID-19 vaccine. This study aim is to evaluate the acceptance of COVID-19 vaccine among population in Qassim region of Saudi Arabia. Methods: This cross-sectional study conducted among target people who were more than 11 years old in Qassim Region of Saudi Arabia using the snowball sample study. A self-administered online questionnaire was used that evaluates the knowledge and acceptance of COVID-19 vaccine among this population. Results: The results show that the participants' high knowledge of COVID-19 translates into good and safe practices, during the COVID-19 pandemic. Public health workers worldwide should concentrate on enlightening and building faith among the unsure and reluctant population regarding security, effectiveness, and adverse effects of the COVID-19 vaccine. Conclusions: The study findings are useful to the policymakers and healthcare professionals who are working on vaccine awareness programs of COVID-19. The findings conclude that the health education interventions should be directed to population of Qassim, Saudi Arabia, at high risk of contracting COVID-19. [ABSTRACT FROM AUTHOR]

The present study was conducted to investigate the allelopathic activity of Rumex dentatus aqueous leaf extract on metabolites, reactive oxygen species (ROS), and enzymes activities in Portulaca oleraceae. The results showed a reduction of carbohydrates, protein, DNA, and RNA contents. However, the total phenolics, total flavonoids, and malondialdehyde were enhanced. The ROS such as hydrogen peroxide, superoxide radicals, and hydroxyl radicals were increased in concentration-dependent manner. The activities of glucose-6-phosphate dehydrogenase, 6-phosphoglucnate dehydrogenase, nitrate reductase, and glutamine synthetase were reduced in Portulaca leaves as the concentration of Rumex extract increased; however the activities of NADH-oxidase and phenylalanine ammonia-lyase were increased under treatment with the various tested concentrations of Rumex extract. Gas chromatography–mass spectrometry profile of aqueous Rumex extract was performed for the detection of the dominant compounds that may reveal the impact on Portulaca metabolites and enzymes activities. [ABSTRACT FROM AUTHOR]

An in silico approach applying computer-simulated models helps enhance biomedicines by sightseeing the pharmacology of potential therapeutics. Currently, an in silico study combined with in vitro assays investigated the antimicrobial ability of Limoniastrum monopetalum and silver nanoparticles (AgNPs) fabricated by its aid. AgNPs mediated by L. monopetalum were characterized using FTIR, TEM, SEM, and DLS. L. monopetalum metabolites were detected by QTOF–LCMS and assessed using an in silico study for pharmacological properties. The antibacterial ability of an L. monopetalum extract and AgNPs was investigated. PASS Online predictions and the swissADME web server were used for antibacterial activity and potential molecular target metabolites, respectively. Spherical AgNPs with a 68.79 nm average size diameter were obtained. Twelve biomolecules (ferulic acid, trihydroxy-octadecenoic acid, catechin, pinoresinol, gallic acid, myricetin, 6-hydroxyluteolin, 6,7-dihydroxy-5-methoxy 7-O-β-d-glucopyranoside, methyl gallate, isorhamnetin, chlorogenic acid, 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4-oxo-4H-chromen-3-yl 6-O-(6-deoxy-β-l-mannopyranosyl)-β-d-glucopyranoside) were identified. The L. monopetalum extract and AgNPs displayed antibacterial effects. The computational study suggested that L. Monopetalum metabolites could hold promising antibacterial activity with minimal toxicity and an acceptable pharmaceutical profile. The in silico approach indicated that metabolites 8 and 12 have the highest antibacterial activity, and swissADME web server results suggested the CA II enzyme as a potential molecular target for both metabolites. Novel therapeutic agents could be discovered using in silico molecular target prediction combined with in vitro studies. Among L. Monopetalum metabolites, metabolite 12 could serve as a starting point for potential antibacterial treatment for several human bacterial infections. [ABSTRACT FROM AUTHOR]

This study investigated the prevalence, antibiogram, virulence, extendedspectrum β-lactamases (ESBLs), and non-β-lactam encoding genes of Proteus species isolated from infected dogs in Ismailia province, Egypt. The study was conducted on 70 fecal swabs collected from dogs with diarrhea for bacteriological identification of Proteus spp. The positive isolates were evaluated for antibiotic susceptibility, molecular tests of virulence, ESBLs, and non-β-lactam encoding genes. Prevalence of Proteus spp. was 35.7% (25/70), including Proteus mirabilis (n = 23) and Proteus vulgaris (n = 2). The Proteus spp. prevalence revealed diversity, higher in males than females, in ages < 12 weeks. Investigation of antimicrobial resistance was found against penicillin and amoxicillin (100%), amoxicillin--clavulanic acid (32%), cephalosporins: cefotaxime and ceftazidime (36%), and monobactam: aztreonam (28%) as ESBLs, in addition to tetracycline (32%) and trimethoprim sulfamethoxazole (100%). The strains retrieved by PCR revealed ureC, zapA, and rsbA virulence genes with variant prevalence as 92%, 60%, and 52%, respectively. In addition, the recovered strains contained ESBL genes with a dramatic variable prevalence of 100%, 92%, 36%, and 32%, to blaTEM, blaSHV, blaCTX-M, and blaOXA-1, respectively, and non β-lactam encoding genes with a prevalence of 100%, 48%, 44%, 20%, and 12%, to sul1, tetA, intI1, qnrA, and aadA1. Moreover, 28% (7/25) of recovering strains were MDR (multidrugresistant) up to four classes of antimicrobials, and 48% (12/25) of the examined strains were MDR up to three antimicrobial classes. In conclusion, to the best of our knowledge, our study could be the first report recording MDR Proteus spp. in dogs in Egypt. [ABSTRACT FROM AUTHOR]

Synthetic chemical preservatives are widely used in the food industry to delay the deterioration caused by microbial growth, enzyme activities and oxidation reactions. The last few decades have witnessed marked interest in finding natural food preservatives due to the potential health damage of synthetic preservatives; consumers have become skeptical of consuming foods containing these additives. Polyphenols used as natural preservatives that can be extracted from fruits, vegetables, herbs and spices provide the best alternative for partial or complete replacement of their synthetic analogues. The present study's emphasis was on employing different plant extracts to be efficiently used as antimicrobial agents for developing replacements for the synthetic chemical additives in food products. The study also investigated the antimicrobial potentialities of five medicinal plants, widely used in Egypt (sumac, tamarind, rosemary, roselle and lemon) against six microbial markers (E. coli, P. aeruginosae, B. subtilis, S. aureus, Penicillium sp. and A. niger.). Sumac extracts showed the best activity against all tested microorganisms, producing the widest inhibition zones ranging from 14 to 45 mm, followed by tamarind and roselle extracts, with inhibition zones ranging from 8–36 and 8–34 mm, respectively. On the other hand, extracts of rosemary and lemon showed variable antimicrobial activity. All extracts from all tested plants were less active against fungal species than bacterial species. In all cases, the organic extracts (80% methanol, 80% ethanol) showed the same or greater activity than the aqueous extracts. In addition, the methanolic extracts showed the strongest and broadest spectrum. The most sensitive strain to plant extracts was B. subtilis, while the most resistant strain was P. aeruginosae. The MIC and MBC or MFC values of methanolic extracts were assayed using the broth dilution method. Sumac extract showed the best activity against all tested microorganisms with the lowest values of MIC and MBC or MFC (from 0.260 to 0.877 and 0.310 to 1.316 mg/mL, respectively, for bacteria, and from 1.975 to 2.5 and 2.5 to 4.444 mg/mL, respectively, for fungi). Interestingly, the tested extracts inhibited microbial growth in tomato paste and pasteurized cow milk for a long storage period (increase shelf life) as compared to the control samples. In conclusion, herbal and spice extracts could be successfully applied as natural antimicrobials for the elimination of food borne microbes and pathogen growth. [ABSTRACT FROM AUTHOR]

The current study aimed to produce synbiotic cheese, adding inulin and Bifidobacterium animalis subsp. lactis as prebiotics and probiotics, respectively. The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening. The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed. Scanning electron microscopy (SEM) of the cheese revealed that inulin could improve the cheese structure. Meanwhile, inulin increased the likeliness of the cheese, and its probiotic viability remained above 107 colony forming unit (CFU) per gram during ripening. [ABSTRACT FROM AUTHOR]

Frozen yoghurt is a refreshing and nutritious dessert, with or without the flavour that combines the texture of ice cream and yoghurt. Several previous studies have been conducted on Jerusalem artichoke tubers due to their components, which contain inulin compounds and other nutrients with beneficial properties of fresh yoghurt. However, limited studies explored the potential benefits of the addition of Jerusalem artichoke tuber powder as a fat replacer on the physicochemical properties and survival of probiotics in frozen yoghurt. In this respect, the aim of this study was to determine the effect of Jerusalem artichoke tuber powder (JATP) (0, 5, 10, 15, and 20% w/w) of the fat source used in the mix as a fat, and sugar replacer in frozen yoghurt production. The microbiological, physicochemical, textural, and sensory properties of frozen yoghurt were investigated. Samples with JATP contained viable counts of bifidobacterium bifidum BGN4 and Lactobacillus casei Lc-01 of 7 log cfu/g during 90 days of storage, as compared to the control sample. The highest viability of probiotics was obtained in the sample formulated with 10% JATP. The formulation of frozen yoghurt with JATP increased the acidity and enhanced the overrun. Compared with the control sample, the incorporation of JATP into frozen yoghurt increased the melting resistance, overrun, and viscosity of the frozen yoghurt. The addition of JATP up to 10% significantly increased sensory attributes. Collectively, the study concluded that the enrichment of frozen yoghurt with JATP up to 20% will provide consumers with health benefits and could be introduced to markets as functional frozen yoghurt. [ABSTRACT FROM AUTHOR]

Background: Among diabetes complications, diabetic foot disease (DFD) is the most common and the most preventable complication. This study aimed to assess the level of knowledge, attitude and practice of foot care among type two diabetes mellitus (DM) patients attending the Diabetic and Endocrine Center at King Fahad Specialist Hospital in Buraydah‑Qassim region, Saudi Arabia. Methods: A descriptive cross‑sectional facility‑based study was conducted randomly among type two diabetic patients; respondents were 260 patients. Participants were interviewed using a pretested semi‑structured questionnaire. Results: Of the total studied patients, 54.2% were males with median age of 58 years. Majority 56.5% of patients had good knowledge and 56.9% had good practices regarding diabetic foot care. The mean knowledge score was 4.0 (±1.86) out of 6. About 39.2% of participants received advice regarding foot care from their physicians. Only 41.5% of the patients examined their feet daily, 41.9% carefully dried between the toes after washing, and 40.8% were walking barefooted at home. About 68.5% of the participants had a history of diabetic foot complications. A significant statistical association was found between the good knowledge and patients’ age, educational level, family monthly income, duration of diabetic illness and having prior knowledge regarding foot care (P‑value <0.05). While, good practice of the participants towards the diabetic foot care was found to be statistically associated with the family monthly income and the prior knowledge regarding diabetic foot care (P‑value <0.05). Conclusion: Our study revealed that more than half of the participants had good knowledge and practices of diabetic foot care. However, the role of physicians and medical staff in annual foot examination and health education is crucial. An awareness program implementation for diabetic foot care is highly needed. [ABSTRACT FROM AUTHOR]

There is an error in affiliation 4 for author Duaa Alammari. The correct affiliation 4 is: College of Public Health and Health Informatics, King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), King Abdullah International Medical Research Center, Riyadh, Saudi Arabia.By Rakan K. Alhabib; Noara Alhusseini; Anas G. Aboalsamh; Ghaith Adi; Aya Ismail; Amro Hajja; Duaa Alammari; Ziad Khalil; Maha A. Alharbi and Sarah K. AlbahitiReported by Author; Author; Author; Author; Author; Author; Author; Author; Author; Author [Extracted from the article]

The definitive screening design (DSD) and artificial neural network (ANN) were conducted for modeling the biosorption of Co(II) by Pseudomonas alcaliphila NEWG-2. Factors such as peptone, incubation time, pH, glycerol, glucose, K2HPO4, and initial cobalt had a significant effect on the biosorption process. MgSO4 was the only insignificant factor. The DSD model was invalid and could not forecast the prediction of Co(II) removal, owing to the significant lack-of-fit (P < 0.0001). Decisively, the prediction ability of ANN was accurate with a prominent response for training (R2 = 0.9779) and validation (R2 = 0.9773) and lower errors. Applying the optimal levels of the tested variables obtained by the ANN model led to 96.32 ± 2.1% of cobalt bioremoval. During the biosorption process, Fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy, and scanning electron microscopy confirmed the sorption of Co(II) ions by P. alcaliphila. FTIR indicated the appearance of a new stretching vibration band formed with Co(II) ions at wavenumbers of 562, 530, and 531 cm–1. The symmetric amino (NH2) binding was also formed due to Co(II) sorption. Interestingly, throughout the revision of publications so far, no attempt has been conducted to optimize the biosorption of Co(II) by P. alcaliphila via DSD or ANN paradigm. [ABSTRACT FROM AUTHOR]

Recently, exogenous α-Lipoic acid (ALA) has been suggested to improve the tolerance of plants to a wide array of abiotic stresses. However, there is currently no definitive data on the role of ALA in wheat plants exposed to sodic alkaline stress. Therefore, this study was designed to evaluate the effects of foliar application by ALA at 0 (distilled water as control) and 20 µM on wheat seedlings grown under sodic alkaline stress (50 mM 1:1 NaHCO3 & Na2CO3; pH 9.7. Under sodic alkaline stress, exogenous ALA significantly (p ≤ 0.05) improved growth (shoot fresh and dry weight), chlorophyll (Chl) a, b and Chl a + b, while Chl a/b ratio was not affected. Moreover, leaf relative water content (RWC), total soluble sugars, carotenoids, total soluble phenols, ascorbic acid, K and Ca were significantly increased in the ALA-treated plants compared to the ALA-untreated plants. This improvement was concomitant with reducing the rate of lipid peroxidation (malondialdehyde, MDA) and H2O2. Superoxide dismutase (SOD) and ascorbate peroxidase (APX) demonstrated greater activity in the ALA-treated plants compared to the non-treated ones. Conversely, proline, catalase (CAT), guaiacol peroxidase (G-POX), Na and Na/K ratio were significantly decreased in the ALA-treated plants. Under sodic alkaline stress, the relative expression of photosystem II (D2 protein; PsbD) was significantly up-regulated in the ALA treatment (67% increase over the ALA-untreated plants); while Δ pyrroline-5-carboxylate synthase (P5CS), plasma membrane Na+/H+ antiporter protein of salt overly sensitive gene (SOS1) and tonoplast-localized Na+/H+ antiporter protein (NHX1) were down-regulated by 21, 37 and 53%, respectively, lower than the ALA-untreated plants. These results reveal that ALA may be involved in several possible mechanisms of alkalinity tolerance in wheat plants. [ABSTRACT FROM AUTHOR]

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Aims: This study aims to synthesize, characterize and apply gold–silver core–shell nanoparticles (Au@Ag NPs), a nanocatalyst, to maximize biodiesel production from fungal isolate Fusarium solani (FS12) via a transesterification one‐step reaction. Methods and Results: The Au@Ag NPs structure was examined by UV‐vis spectrophotometer, transmission electron microscopy, X‐ray diffraction and Fourier transform infrared (FTIR). All devices were used to characterize Au@Ag NPs and confirmed successful synthesis of nanoparticles. Fungal lipid was quantitatively determined by sulfo‐phospho‐vanillin colorimetric method. Among 15 F. solani isolates obtained from rhizospheric soils of the date palm, F. solani (AF12) was chosen as the highly significant producer that accumulates above 20% lipid. The maximum biodiesel yield was 91.28 ± 0.19%, obtained under the optimum reaction conditions of 3% Au@Ag NPs as nanocatalyst concentration, and 1:20 oil to methanol molar ratio at 70℃ for 30 min. HPLC method was applied for monitoring in situ transesterification reaction. FTIR spectroscopy was used in qualitative analysis of biodiesel by verifying the presence of unique characteristic peaks of diagnostic significance. The quality of the biodiesel produced was confirmed by the high purity of fatty acid methyl esters analysis content up to >99%. Conclusions: These findings propose the applicability of F. solani (FS12) as a promising isolate to accumulate lipids and biodiesel production as a feedstock. Significance and Impact of the Study: The link between nanotechnology and fungi. Au@Ag NPs were synthesized at room temperature, which displayed high catalytic activity for in situ transesterification reaction. Catalytic activity appeared at low temperature, mole ratio and short reaction time. Oleaginous fungi are described as easily grown, have short life cycle, are cost‐effective, and they utilized various sources of carbon up to waste and a simplified process to develop scale‐up production as well, economic value, opposite the usage of vegetable oils which need for large agricultural areas. [ABSTRACT FROM AUTHOR]

As an anticancer medication, L-asparaginase enzyme from bacteria has been applied as an efficient therapeutic agent for acute lymphoblastic leukemia (ALL) treatment. Current investigation was designed to optimize and partially purify the L-as-paraginase from Bacillus safensis. Bacterial growth conditions such as nutrition, pH, temperature, inoculum size and incubation periods have been examined for optimal L-asparaginase production. Findings revealed that, maximum L-asparaginase activity from Bacillus safensis was 123.53 U/min/ml that obtained after 48h at 35±2°C of incubation in shaking incubator (150 rpm). The best tested culture (inoculum size, 30%) was supplemented with L-asparagine (0.75%) for B. safensis in the presence of yeast ex-tract (0.25 %) without carbon source at pH 8. There-fore, he most positive significant independent factors influencing enzyme production are time, temperature, pH, inoculum size, and agitation speed. The crude enzyme was extracted from B. safensis free cell that filtered and partially purified by ammonium sulphate (80%). In conclusion: Bacillus safensis showed its ability as promising candidate to produce L-asparaginase as well as enzyme activity was maximized at nutrition medium without carbon sources. [ABSTRACT FROM AUTHOR]

By using the indigenous micro-organisms of the polluted environment to be treated, bioremediation can be a successful strategy. PCR and RT-PCR molecular techniques were applied to examine the evolution of fungal isolates through putative genes f ligninolytic enzymes like lignin peroxidase (LiP), laccase (LaC), manganese peroxidase (MnP), and cellulase (Cx) as a response to polluting of the environment by hydrocarbons. In this study, isolation of rhizospheric fungal isolates, molecular identification, crude oil tolerance, and enzyme excretions were demonstrated. From the date palm rhizosphere, 3 fungal isolates were isolated and characterized morphologically and molecularly by ITS ribosomal RNA (rRNA) sequencing. The isolates were identified as Aspergillus flavus AF15, Trichoderma harzianum TH07, and Fusarium solani FS12 through using the BLAST tool in NCBI. All fungal isolates showed high tolerance to crude oil and survived with various responses at the highest concentration (20%). Aspergillus flavus AF15 and Trichoderma harzianum TH07 demonstrated promising oil-degrading tolerance ability based on the dose inhibition response percentage (DIRP) of the fungal isolates. A. flavus had a powerful capacity to production Cx, LaC, LiP and MnP with a range from 83.7 to 96.3 mL. Molecularly, nine genes of the ligninolytic enzymes, cbh (cbhI.1 , cbhI.1 , cbhII) lcc , lig (1, 2, 4 and 6) and mnp were tested for presence and expression (by PCR and RT-PCR, respectively). PCR showed that all isolates contained all the nine genes examined, regardless of capacity to enzymes production profiles, so the presence responses of nine genes did not correlate with enzymes-production ability. Gene expression analysis shows a more diverse pattern for tested isolates for example, Aspergillus flavus AF15 had over-expression of lig and mnp genes, Fusarium solani FS12 have a weak signal with lcc gene while, Trichoderma harzianum TH07 showed moderate expression of mnp and lcc genes. The power of the transcription of the gene leads to increased enzyme secretion by fungal isolates. Fungi are important microorganisms in the clean-up of petroleum pollution. They have bioremediation highly potency that is related to their diverse production of these catalytic enzymes. [ABSTRACT FROM AUTHOR]

This investigation displayed the good catalytic activity of silver nanoparticles (AgNPs) on the reduction of methylene blue dye. During this work, Honey was chosen for environmentally reducing and stabilizing agents for preparation of silver nanoparticles then characterized these nanoparticles by ultraviolet–visible spectroscopy (UV–Vis), functional biomolecules were confirmed by Fourier transform infrared spectroscopy (FTIR). Via transmission electron microscopy (TEM), the size and shape of silver nanoparticles revealed that the particles are spherical and monodispersed without major agglomeration, the particle size ranging from 5 to 25 nm, in addition, the largest particle density levels are 5–10 nm, ZETA Seizers studied the size distribution of the colloidal solution. UV/Vis spectrophotometer and HPLC were used to study and analyze the degradation performance of silver nanoparticles on methylene blue. The results show that 92% of methylene blue has been degraded after 72 h. additionally, several new peaks have appeared after treatment of the samples by using HPLC. [ABSTRACT FROM AUTHOR]

• Frontal mucoceles in children are rare. • A child with lateral frontal mucocele with no known etiology. • This shows the usefulness of image-guided endoscopic sinus surgery. The aim of this report is to show the usefulness of endoscopic sinus surgery in management of lateral frontal mucocele in pediatric patient. A 14 years old girl presented with right frontal bone depression and headache. CT and MRI showed lateral mucocele occupying the right frontal sinus. The patient was managed successfully by image-guided endoscopic sinus surgery and she was well after two years follow up. Frontal mucoceles in children are rare. We report a rare case of a child with lateral frontal mucocele with no known etiology, treated successfully by image-guided endoscopic sinus surgery with no recurrence after two years follow-up. This case shows the usefulness of image-guided endoscopic sinus surgery in treatment of lateral frontal mucocele in children. [ABSTRACT FROM AUTHOR]

A polyphasic approach consisting of physical, chemical and molecular characterization was applied to three Alternaria species, A. alternata, A. chlamydospora and A. solani, to characterize and identify isolates producing and non-producing silver nanoparticles (AgNPs). Based on physical characters, the synthesized AgNPs were characterized by transmission electron microscopy and formation of spherical AgNPs sized 5 nm to 60 nm. Scanning electron microscopy (SEM) of the AgNPs did not show a uniform surface; the shape was irregular, like a coral reef. Energy Dispersive Spectroscopy (EDS) showed the presence of Ag element. Fourier transform-infrared spectroscopy (FTIR) confirmed the conversion of Ag ions to AgNPs by fungal metabolites. A. alternata PNU75 non-producing AgNPs showed negative results when characterized by physical devices. Chemical characterization involved the screening of three Alternaria species for fatty acids (FAs) with similar FAs composition while different in terms of relative concentration. The principal FAs were palmitic, stearic, oleic acid and linoleic acid, which comprised 90% or more of the total FAs composition of these species. Molecularly, the fungal protein pattern of the three Alternaria species showed changes against the control by finding 6 proteins associated with AgNPs-producing species. Inter simple sequence repeats (ISSR) analysis using ten primers indicated a high level of genetic variability of three Alternaria species producing and non-producing AgNPs. The results also indicated that the AgNPs synthesized by A. alternata at the concentration 100 mg/L gave the highest inhibition zone against Aspegillus flavus and Aaltenaria alternata, where the percentages of reduction were 77.1% and 87.6% respectively. [ABSTRACT FROM AUTHOR]

The growth of the marine green alga Dunaliella parva was studied and optimized under different salinity levels of NaCl (0.5, 1, 2, 2.5, and 3.5 M). The growth was monitored by cell number pigment content (Chl. a, Chl. b, and carotenoids). The grown alga, under the optimal conditions, was exposed to different stresses (nitrogen, phosphorus starvation, and salinity either singly or combined. Under nitrogen and phosphorus starvation, either singly or combined, the growth rate and the metabolic activities were decreased. Under salt stress (2.5 M NaCl) combined with N starvation and heavy metals stress, glycerol production increased, while glycerol synthesis decreased under salt stress of 1 M NaCl and P starvation. Also, free radicals (total antioxidant, reducing power, DPPH, and Lipid peroxidation), pigment content, and activity of antioxidant enzymes were recorded. D. parva grown under salinity level (2.5 M NaCl) combined with nutrient starvation correlated with more efficient enzymatic antioxidant activity accumulation. This study strongly suggested that the induction of antioxidant defense was one component of the tolerance mechanism of D. parva to salinity, as evidenced by its growth behavior. [ABSTRACT FROM AUTHOR]

Aim: In the recent years, many HLA laboratories predict crossmatch results based on the strength of Donor Specific Antibodies (DSA) measured by single antigen (SA) solid phase assays. This study evaluates the prediction of CDC crossmatch results to flow cytometry Median Channel Sshift (MCS) versus SA cumulative Median Fluorescent Intensity (MFI) of DSA. Methods: 93 T cell AHG-CDC and flow cytometry crossmatch (FCXM) were reviewed, results compared to DSA MFI (0-10000) (>10000). In the other hand, the same samples were compared to MCS (0-250)& (>250). Percentage frequency, chi-square and P-value were calculated to assess the association between mentioned variables. Results: DSA MFI’s and MCS were grouped into two groups (0-10000), (>10000), (0-250) & (>250) respectively and are presented in this table.[Table 1] Conclusions: From the above data there was a significant association in prediction of positive CDC crossmatch based on MCS (chi square of 67.95, P<.0001) compared to SA cumulative MFI (Chi square 11.38, P=0.0007. The positive predictive value of the CDC crossmatch based on MCS was higher than MFI with 88% and 57% respectively. Therefore SA cumulative MFI is not significant to consider as predictor for a positive CDC crossmatch rather than transplant risk factor. [Copyright &y& Elsevier]

Abstract: Donor-specific antibodies (DSA) in sera of sensitized transplant patients are often produced against the specific epitopes on mismatched HLA antigens. In this study, we selected sera from 30 kidney transplant patients with DSA and AMR to define DQ epitopes. Using adsorption and elution assays, we identified 18 antibody reaction patterns to define 6 new epitopes and to confirm 12 previously defined epitopes. In one patient case, one mismatched antigen produced 3 different antibodies and, in another, antibodies were produced against the alpha and beta chains of the same antigen. For some sera, a single epitope can explain reactions for 27 of the 29 DQ beads in the single antigen panel. Several studies highlighted the prevalence of anti-DQ antibodies. In 2011, Almeshari et al. observed DQ DSA in 34/46 (74%) of rejection episodes – 44 patients had DSA and 20 lost their graft due to AMR. Other studies have shown a high prevalence of anti-DQ antibodies and an association with adverse effects on the graft. We conclude that analysis of the epitopes of the DQ antibodies using Adsorption/Elution and testing on single antigen DQ beads helps to better understand the specificities and cross-reactions of DQ antibodies in transplant patients. [Copyright &y& Elsevier]

Aim: In 2008, we defined 18 HLA DQ epitopes using mouse monoclonal antibodies and alloantibodies adsorbed and eluted (Ads/Elut) from HLA DQ homozygous cell lines. Here, we confirm 11 of the 18 epitopes and define 10 NEW epitopes of alloantibodies purified by Ads/Elut using recombinant single antigen cell lines. Methods: Alloantibodies were purified from the sera of 36 kidney transplant patient by Ads/Elut using HLA DQ recombinant single antigen cell lines (rHLA-DQ). Each serum was Ads/Elut using several different cell lines to insure monoclonality. Eluted antibody specificities were determined by the HLA Class II Single Antigen (SA) beads. Epitope search software was used to identify amino acids exclusively shared among all positive DQ heterodimers (DQA or DQB) on the SA beads. These amino acids define the epitope. Results: Ten new HLA DQ epitopes, 2 Alpha & 10 Beta chain, are defined (partial list in table below) and eleven previously defined DQ epitopes were confirmed. Donor Specific Antibodies (DSA) were found in almost all sera. And in most sera, we found more than one antibody positive with the same mismatched antigen, but each targets a different epitope. When anti DR or DP antibodies were present in the sera, the eluted antibodies were negative with the DR and DP beads indicating only DQ-specificities. Partial list of new DQA,B epitopes. nk = not known, nn = not needed, na = not applicable, xx = exact allele not known Conclusions: Using rHLA-DQ single antigen cell lines to purify anti HLA-DQ antibodies and testing the antibodies with the HLA-DQ single antigen beads proved very effective in defining DQA and DQB epitopes. [Copyright &y& Elsevier]

Fungi play a significant role in the deterioration of various types of monuments. Therefore, the protection of ancient monuments from fungal attacks is an important goal that must attract the attention of researchers worldwide. A total of 69 fungal isolates were recovered from 22 deteriorated objects compromising paper, textiles, wood, and stone in the National Museum of Egyptian Civilization (NMEC) storeroom, Cairo, Egypt. The isolates were identified as 12 different species categorized into three different genera, namely, Aspergillus (9 species), Penicillium (2 species) and Trichoderma (1 species). Among them, Aspergillus fumigatus was the most prevalent species. Three essential oils were assessed for antifungal activity and compared with the antifungal effects of five synthetic microcides to identify a natural inhibitory treatment. Thyme oil and sodium azide were found to be the most active growth inhibitors, with minimum inhibitory concentrations (MICs) of 625 and 100 ppm, with inhibition zone diameters of 19.0 ± 0.70 - 23.76 ± 1.15 and 13.30 ± 0.35 - 19.66 ± 0.54 mm, respectively. An in vitro simulation of the biodeterioration process was conducted using spores of the A. fumigatus strain NMEC-PSTW.1 on model cubes made of paper, textile, wood, and stone materials. The changes in the characteristics of the artificially deteriorated materials were analyzed using environmental scanning electron microscopy/energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy. The results revealed changes in the morphology, physical properties, and chemical composition induced by A. fumigatus NMEC-PSTW.1. Overall, thyme oil is recommended as a natural inhibitor to protect carbonate and cellulosic monuments in NMEC against fungal attack.

Background: Cerebral palsy (CP) is a prevalent nonprogressive disorder that leads to impaired movement (ie, spasticity), posture, and balance, which affects functions such as walking and upper extremity tasks. Current medical treatments show efficacy in improving motor performance but have considerable side effects. Emerging off-label use of central nervous system (CNS) medications for improving motor performance has shown promising results in children with CP and other populations., Objective: The aim of this study is to describe a protocol for a pilot randomized controlled trial (RCT) to examine the safety, tolerability, and efficacy of methylphenidate (MPH) and modafinil on spasticity and motor performance in children with CP., Methods: This will be a protocol study for a pilot, triple-masked, placebo-controlled RCT (a class I trial following the American Academy of Neurology criteria) with blinded patients, outcome assessors, and intervention delivery team. Eligible children should be diagnosed with CP levels I or II based on the Gross Motor Function Classification System and be aged between 7 and 12 years. Thirty-six children with CP will be randomized into 3 groups to receive (1) MPH (2.5 mg of MPH + 100 mg placebo), (2) modafinil (100 mg modafinil + 2.5 mg placebo), or (3) a placebo (2.5 mg placebo + 100 mg placebo), in addition to physical therapy for 12 weeks. Primary outcomes include the Gross Motor Function Measure-66 and the Modified Ashworth Scale. Secondary outcomes include the Timed Up and Go test, 5 Time Sit to Stand test, Modified Clinical Test for Sensory Interaction of Balance, and 10-Meter Walk Test., Results: The protocol has been accepted by Kuwait University (VDR/EC-225) and the Ministry of Health of Kuwait (2022/2157). The inclusion of participants will start in June 2024., Conclusions: The combination of CNS stimulant medications and controlling for rehabilitation has not been studied yet. The findings of this study may determine if using CNS stimulant medications is beneficial for the reduction of spasticity and improvement of physical function in children with spastic CP., Trial Registration: ClinicalTrials.gov NCT05675098; https://clinicaltrials.gov/study/NCT05675098., International Registered Report Identifier (irrid): PRR1-10.2196/53728., (©Mansour Alotaibi, Anwar B Almutairi, Saleh Alhirsan, Afrah Alkazemi, Maha Alharbi, Naif Alrashdi, Ahmad Taqi, Bibi Alamiri, Laura Vogtle, Mohammed M Alqahtani. Originally published in JMIR Research Protocols (https://www.researchprotocols.org), 05.03.2024.)