29 results on '"Alegado, Rosanna A."'
Search Results
2. RISING TOGETHER IN RESPONSE TO THE COVID-19 PANDEMIC
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Kaczor, Keri, Alegado, Rosanna ‘Anolani, Hintzen, Katy, Ching, Lauren, Lieberman, Michael, Chang, Sandra, Nakano, Eileen, Sy, Angela, Horio, David, Tam, Kaeo, Nerurkar, Vivek R., Smyth, Ashley, Collins, Angela, Fallon, Kathleen, Focazio, Paul, and Kreisler, Samantha
- Published
- 2024
3. SPOTLIGHT : A Practitioner-Led Approach to a Climate Change Needs Assessment of Native Hawaiian Aquaculture
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Hintzen, Katy, Asuncion, Brenda, Tamanaha, Miwa, Loko I‘a, Hui Mālama, and Alegado, Rosanna
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- 2024
4. Ho‘okele ka Wa‘a : RECALIBRATING THE SAIL PLAN FOR NATIVE HAWAIIANS AND PACIFIC ISLANDERS IN THE OCEAN SCIENCES
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Kane, Haunani H., Choy, C. Anela, Bruno, Barbara C., Tachera, Diamond K., Keliipuleole, Keku‘iapōiula, Wong-Ala, Jennifer A.T.K., Burns, John H.R., Kapono, Clifford A., Pascoe, Kailey H., Steward, Kainalu, and Alegado, Rosanna ‘Anolani
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- 2023
5. BUILDING DIVERSITY, EQUITY, AND INCLUSION IN THE OCEAN SCIENCES : AUTOBIOGRAPHICAL SKETCHES
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Kappel, Ellen S., Aarons, Sarah M., Alegado, Rosanna ‘Anolani, Bathala, Neeti, Buglass, Salome, Choy, Anela, Clare, Xochitl S., Davis, Jeanette, Garza, Corey, Gibson, Deidre, Girguis, Peter, Hoskins-Brown, Dionne, Jearld, Ambrose, Jones, Brandon, Kane, Haunani Hi‘ilani, Martinez, Catalina, Morris, Vernon R., Navarro, Michael, Padilla-Gamiño, Jacqueline L., Puniwai, Noelani, Ravelo, Christina, Sato, Mei, Sturdivant, S. Kersey, Todd, Wendy F., White, Lisa D., and Wright, Dawn
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- 2023
6. A relational framework for microbiome research with Indigenous communities
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Bader, Alyssa C., Van Zuylen, Essie M., Handsley-Davis, Matilda, Alegado, Rosanna A., Benezra, Amber, Pollet, Rebecca M., Ehau-Taumaunu, Hanareia, Weyrich, Laura S., and Anderson, Matthew Z.
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- 2023
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7. Systemic racial disparities in funding rates at the National Science Foundation
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Chen, Christine Yifeng, Kahanamoku, Sara S, Tripati, Aradhna, Alegado, Rosanna A, Morris, Vernon R, Andrade, Karen, and Hosbey, Justin
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Biological Sciences ,Biomedical and Clinical Sciences ,Health Sciences ,Engineering ,Immunotherapy ,Systemic Racism ,research grants ,institutional funding of science ,National Science Foundation ,racial disparities ,meta-research ,None ,none ,Biochemistry and Cell Biology ,Biological sciences ,Biomedical and clinical sciences ,Health sciences - Abstract
Concerns about systemic racism at academic and research institutions have increased over the past decade. Here, we investigate data from the National Science Foundation (NSF), a major funder of research in the United States, and find evidence for pervasive racial disparities. In particular, white principal investigators (PIs) are consistently funded at higher rates than most non-white PIs. Funding rates for white PIs have also been increasing relative to annual overall rates with time. Moreover, disparities occur across all disciplinary directorates within the NSF and are greater for research proposals. The distributions of average external review scores also exhibit systematic offsets based on PI race. Similar patterns have been described in other research funding bodies, suggesting that racial disparities are widespread. The prevalence and persistence of these racial disparities in funding have cascading impacts that perpetuate a cumulative advantage to white PIs across all of science, technology, engineering, and mathematics.
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- 2022
8. Detection of horizontal gene transfer in the genome of the choanoflagellate Salpingoeca rosetta
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Matriano, Danielle M., Alegado, Rosanna A., and Conaco, Cecilia
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- 2021
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9. Bacterial Influences on Animal Origins
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Alegado, Rosanna A and King, Nicole
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Genetics ,1.1 Normal biological development and functioning ,Underpinning research ,Animals ,Bacteria ,Biological Evolution ,Cell Adhesion ,Choanoflagellata ,Eukaryota ,Phagocytosis ,Phylogeny ,Signal Transduction - Abstract
Animals evolved in seas teeming with bacteria, yet the influences of bacteria on animal origins are poorly understood. Comparisons among modern animals and their closest living relatives, the choanoflagellates, suggest that the first animals used flagellated collar cells to capture bacterial prey. The cell biology of prey capture, such as cell adhesion between predator and prey, involves mechanisms that may have been co-opted to mediate intercellular interactions during the evolution of animal multicellularity. Moreover, a history of bacterivory may have influenced the evolution of animal genomes by driving the evolution of genetic pathways for immunity and facilitating lateral gene transfer. Understanding the interactions between bacteria and the progenitors of animals may help to explain the myriad ways in which bacteria shape the biology of modern animals, including ourselves.
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- 2014
10. Synthesis of the Rosette-Inducing Factor RIF‑1 and Analogs
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Beemelmanns, Christine, Woznica, Arielle, Alegado, Rosanna A, Cantley, Alexandra M, King, Nicole, and Clardy, Jon
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Alkanesulfonic Acids ,Bacteroidetes ,Choanoflagellata ,Lipids ,Molecular Structure ,Morphogenesis ,Stereoisomerism ,Chemical Sciences ,General Chemistry - Abstract
Studies on the origin of animal multicellularity have increasingly focused on one of the closest living relatives of animals, the choanoflagellate Salpingoeca rosetta. Single cells of S. rosetta can develop into multicellular rosette-shaped colonies through a process of incomplete cytokinesis. Unexpectedly, the initiation of rosette development requires bacterially produced small molecules. Previously, our laboratories reported the planar structure and femtomolar rosette-inducing activity of one rosette-inducing small molecule, dubbed rosette-inducing factor 1 (RIF-1), produced by the Gram-negative Bacteroidetes bacterium Algoriphagus machipongonensis. RIF-1 belongs to the small and poorly explored class of sulfonolipids. Here, we report a modular total synthesis of RIF-1 stereoisomers and structural analogs. Rosette-induction assays using synthetic RIF-1 stereoisomers and naturally occurring analogs defined the absolute stereochemistry of RIF-1 and revealed a remarkably restrictive set of structural requirements for inducing rosette development.
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- 2014
11. A bacterial sulfonolipid triggers multicellular development in the closest living relatives of animals.
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Alegado, Rosanna A, Brown, Laura W, Cao, Shugeng, Dermenjian, Renee K, Zuzow, Richard, Fairclough, Stephen R, Clardy, Jon, and King, Nicole
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Bacteroidetes ,Lipids ,Feeding Behavior ,Phylogeny ,Morphogenesis ,Choanoflagellata ,Biological Evolution ,Algoriphagus ,Other ,Salpingoeca rosetta ,bacterial sulfonolipid ,multicellular development ,Lipid Metabolism ,Biochemistry and Cell Biology - Abstract
Bacterially-produced small molecules exert profound influences on animal health, morphogenesis, and evolution through poorly understood mechanisms. In one of the closest living relatives of animals, the choanoflagellate Salpingoeca rosetta, we find that rosette colony development is induced by the prey bacterium Algoriphagus machipongonensis and its close relatives in the Bacteroidetes phylum. Here we show that a rosette inducing factor (RIF-1) produced by A. machipongonensis belongs to the small class of sulfonolipids, obscure relatives of the better known sphingolipids that play important roles in signal transmission in plants, animals, and fungi. RIF-1 has extraordinary potency (femtomolar, or 10(-15) M) and S. rosetta can respond to it over a broad dynamic range-nine orders of magnitude. This study provides a prototypical example of bacterial sulfonolipids triggering eukaryotic morphogenesis and suggests molecular mechanisms through which bacteria may have contributed to the evolution of animals.DOI:http://dx.doi.org/10.7554/eLife.00013.001.
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- 2012
12. Water Back: A Review Centering Rematriation and Indigenous Water Research Sovereignty.
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Leonard, Kelsey, David-Chavez, Dominique, Smiles, Deondre, Jennings, Lydia, Alegado, Rosanna 'Anolani, Tsinnajinnie, Lani, Manitowabi, Joshua, Arsenault, Rachel, Begay, Rene L., Kagawa-Viviani, Aurora, Davis, Dawn D., van Uitregt, Vincent (Billy), Pichette, Hawlii, Liboiron, Max, Moggridge, Bradley, Carroll, Stephanie Russo, Tsosie, Ranalda L., and Gomez, Andrea
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The recent Land Back movement has catalysed global solidarity towards addressing the oppression and dispossession of Indigenous Peoples' Lands and territories. Largely absent from the discourse, however, is a discussion of the alienation of Indigenous Peoples from Water by settler-colonial states. Some Indigenous Water Protectors argue that there cannot be Land Back without Water Back. In response to this emergent movement of Water Back, this review of research by Indigenous and non-Indigenous writers traces the discursive patterns of Indigenous Water relationships and rematriation across themes of colonialism, climate change, justice, health, rights, responsibilities, governance and cosmology. It advances a holistic conceptualization of Water Back as a framework for future research sovereignty, focusing mainly on instances in Canada, Australia, Aotearoa New Zealand, and the United States. We present the findings on the current global Waterscape of Indigenous-led research on Indigenous Water issues. Water Back offers an important framework centring Indigenous ways of knowing, doing, and being as a foundation for advancing Indigenous Water research. [ABSTRACT FROM AUTHOR]
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- 2023
13. A Practitioner-Led Approach to a Climate Change Needs Assessment of Native Hawaiian Aquaculture.
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Hintzen, Katy, Asuncion, Brenda, Tamanaha, Miwa, Loko I'a, Hui Mālama, and Alegado, Rosanna
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NEEDS assessment ,HAWAIIANS ,CLIMATE change adaptation ,AQUACULTURE ,GLOBAL environmental change ,PHYSIOLOGICAL adaptation - Abstract
This article discusses a practitioner-led approach to assessing the climate change needs of Native Hawaiian aquaculture. It highlights the historical significance of loko i'a, traditional fishponds, in supporting food production and the decline of these practices due to colonial capitalism and US imperialism. The article emphasizes the importance of community organizations in restoring Indigenous stewardship and revitalizing customary practices. The needs assessment conducted by the Hui Mālama Loko I'a provides practitioners with a platform for resilience and sets priorities based on community knowledge. The article calls for the inclusion of marginalized community perspectives in climate assessments and emphasizes the importance of grassroots leadership in guiding adaptation actions. [Extracted from the article]
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- 2024
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14. Bacterial lipopolysaccharide induces settlement and metamorphosis in a marine larva.
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Freckelton, Marnie L., Nedved, Brian T., You-Sheng Cai, Shugeng Cao, Helen Turano, Alegado, Rosanna A., and Hadfield, Michael G.
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METAMORPHOSIS ,MARINE natural products ,LIPOPOLYSACCHARIDES ,EXTRACELLULAR vesicles ,LARVAE ,MARINE invertebrates - Abstract
How larvae of the many phyla of marine invertebrates find places appropriate for settlement, metamorphosis, growth, and reproduction is an enduring question in marine science. Biofilm-induced metamorphosis has been observed in marine invertebrate larvae from nearly every major marine phylum. Despite the widespread nature of this phenomenon, the mechanism of induction remains poorly understood. The serpulid polychaete Hydroides elegans is a well established model for investigating bacteria-induced larval development. A broad range of biofilm bacterial species elicit larval metamorphosis in H. elegans via at least two mechanisms, including outer membrane vesicles (OMVs) and complexes of phage-tail bacteriocins. We investigated the interaction between larvae of H. elegans and the inductive bacterium Cellulophaga lytica, which produces an abundance of OMVs but not phage-tail bacteriocins. We asked whether the OMVs of C. lytica induce larval settlement due to cell membrane components or through delivery of specific cargo. Employing a biochemical structure–function approach with a strong ecological focus, the cells and OMVs produced by C. lytica were interrogated to determine the class of the inductive compounds. Here, we report that larvae of H. elegans are induced to metamorphose by lipopolysaccharide produced by C. lytica. The widespread prevalence of lipopolysaccharide and its associated taxonomic and structural variability suggest it may be a broadly employed cue for bacterially induced larval settlement of marine invertebrates. [ABSTRACT FROM AUTHOR]
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- 2022
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15. Synergy among Microbiota and Their Hosts: Leveraging the Hawaiian Archipelago and Local Collaborative Networks To Address Pressing Questions in Microbiome Research.
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Hynson, Nicole A., Frank, Kiana L., Alegado, Rosanna A., Amend, Anthony S., Arif, Mohammad, Bennett, Gordon M., Jani, Andrea J., Medeiros, Matthew C. I., Mileyko, Yuriy, Nelson, Craig E., Nguyen, Nhu H., Nigro, Olivia D., Prisic, Sladjana, Sangwoo Shin, Daisuke Takagi, Wilson, Samuel T., and Yew, Joanne Y.
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- 2018
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16. Large-scale climatic effects on traditional Hawaiian fishpond aquaculture.
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McCoy, Daniel, McManus, Margaret A., Young, Charles, D'Andrea, Brandon, Ruttenberg, Kathleen C., Alegado, Rosanna ʻAnolani, Kotubetey, Keliʻiahonui, and Kawelo, Angela Hiʻilei
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AQUACULTURE ,CLIMATE change ,FISH populations ,HYPOXIA (Water) ,FISH mortality ,FISH ponds ,FISH industry - Abstract
Aquaculture accounts for almost one-half of global fish consumption. Understanding the regional impact of climate fluctuations on aquaculture production thus is critical for the sustainability of this crucial food resource. The objective of this work was to understand the role of climate fluctuations and climate change in subtropical coastal estuarine environments within the context of aquaculture practices in Heʻeia Fishpond, Oʻahu Island, Hawaiʻi. To the best of our knowledge, this was the first study of climate effects on traditional aquaculture systems in the Hawaiian Islands. Data from adjacent weather stations were analyzed together with in situ water quality instrument deployments spanning a 12-year period (November 2004 –November 2016). We found correlations between two periods with extremely high fish mortality at Heʻeia Fishpond (May and October 2009) and slackening trade winds in the week preceding each mortality event, as well as surface water temperatures elevated 2–3°C higher than the background periods (March-December 2009). We posit that the lack of trade wind-driven surface water mixing enhanced surface heating and stratification of the water column, leading to hypoxic conditions and stress on fish populations, which had limited ability to move within net pen enclosures. Elevated water temperature and interruption of trade winds previously have been linked to the onset of El Niño in Hawaiʻi. Our results provide empirical evidence regarding El Niño effects on the coastal ocean, which can inform resource management efforts about potential impact of climate variation on aquaculture production. Finally, we provide recommendations for reducing the impact of warming events on fishponds, as these events are predicted to increase in magnitude and frequency as a consequence of global warming. [ABSTRACT FROM AUTHOR]
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- 2017
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17. Response of diatom-associated bacteria to host growth state, nutrient concentrations, and viral host infection in a model system.
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Baker, Lydia J., Alegado, Rosanna A., and Kemp, Paul F.
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VIRUS diseases , *ECOLOGICAL succession , *NAVICULA , *BACTERIA , *BACTERIAL communities , *DIATOMS - Abstract
Diatoms are photosynthetic unicellular eukaryotes found ubiquitously in aquatic systems. Frequent physical associations with other microorganisms such as bacteria may influence diatom fitness. The predictability of bacterial--diatom interactions is hypothesized to depend on availability of nutrients as well as the physiological state of the host. Biotic and abiotic factors such as nutrient levels, host growth stage and host viral infection were manipulated to determine their effect on the ecological succession of bacterial communities associated with a single cell line of Chaetoceros sp. KBDT20; this was assessed using the relative abundance of bacterial phylotypes based on 16S rDNA sequences. A single bacterial family, Alteromonadaceae, dominated the attached-bacterial community (84.0%), with the most prevalent phylotypes belonging to the Alteromonas and Marinobacter genera. The taxa comprising the other 16% of the attached bacterial assemblage include Alphaproteobacteria, Betaproteobacteria, Bacilli, Deltaproteobacteria, other Gammaproteobacteria and Flavobacteria. Nutrient concentration and host growth stage had a statistically significant effect on the phylogenetic composition of the attached bacteria. It was inferred that interactions between attached bacteria, as well as the inherent stochasticity mediating contact may also contribute to diatom--bacterial associations. [ABSTRACT FROM AUTHOR]
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- 2016
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18. The two-component sensor kinase KdpD is required for Salmonella typhimurium colonization of Caenorhabditis elegans and survival in macrophages.
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Alegado, Rosanna A., Chin, Chui-Yoke, Monack, Denise M., and Tan, Man-Wah
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SALMONELLA typhimurium , *CAENORHABDITIS elegans , *MACROPHAGES , *PROTEIN kinases , *PATHOGENIC microorganisms , *MICROBIAL virulence , *GENETIC mutation , *NEMATODES - Abstract
Summary The ability of enteric pathogens to perceive and adapt to distinct environments within the metazoan intestinal tract is critical for pathogenesis; however, the preponderance of interactions between microbe- and host-derived factors remain to be fully understood. Salmonella enterica serovar Typhimurium is a medically important enteric bacterium that colonizes, proliferates and persists in the intestinal lumen of the nematode Caenorhabditis elegans. Several Salmonella virulence factors important in murine and tissue culture models also contribute to worm mortality and intestinal persistence. For example, PhoP and the virulence plasmid pSLT are virulence factors required for resistance to the C. elegans antimicrobial peptide SPP-1. To uncover additional determinants required for Salmonella typhimurium pathogenesis in vivo, we devised a genetic screen to identify bacterial mutants defective in establishing a persistent infection in the intestine of C. elegans. Here we report on identification of 14 loci required for persistence in the C. elegans intestine and characterization of KdpD, a sensor kinase of a two-component system in S. typhimurium pathogenesis. We show that kdpD mutants are profoundly attenuated in intestinal persistence in the nematode and in macrophage survival. These findings may be attributed to the essential role KdpD plays in promoting resistance to osmotic, oxidative and antimicrobial stresses. [ABSTRACT FROM AUTHOR]
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- 2011
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19. Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosetta
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Dayel, Mark J., Alegado, Rosanna A., Fairclough, Stephen R., Levin, Tera C., Nichols, Scott A., McDonald, Kent, and King, Nicole
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CELL differentiation , *MORPHOGENESIS , *CYTOLOGY , *FLAGELLATA , *CELL communication , *ELECTRON microscopy , *CARRIER proteins - Abstract
Abstract: It has been posited that animal development evolved from pre-existing mechanisms for regulating cell differentiation in the single celled and colonial ancestors of animals. Although the progenitors of animals cannot be studied directly, insights into their cell biology may be gleaned from comparisons between animals and their closest living relatives, the choanoflagellates. We report here on the life history, cell differentiation and intercellular interactions in the colony-forming choanoflagellate Salpingoeca rosetta. In response to diverse environmental cues, S. rosetta differentiates into at least five distinct cell types, including three solitary cell types (slow swimmers, fast swimmers, and thecate cells) and two colonial forms (rosettes and chains). Electron microscopy reveals that cells within colonies are held together by a combination of fine intercellular bridges, a shared extracellular matrix, and filopodia. In addition, we have discovered that the carbohydrate-binding protein wheat germ agglutinin specifically stains colonies and the slow swimmers from which they form, showing that molecular differentiation precedes multicellular development. Together, these results help establish S. rosetta as a model system for studying simple multicellularity in choanoflagellates and provide an experimental framework for investigating the origin of animal multicellularity and development. [Copyright &y& Elsevier]
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- 2011
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20. Resistance to antimicrobial peptides contributes to persistence of Salmonella typhimurium in the C. elegans intestine.
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Alegado, Rosanna A. and Man-Wah Tan
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SALMONELLA typhimurium , *ANTIMICROBIAL peptides , *ANTI-infective agents , *CAENORHABDITIS elegans , *SALMONELLA diseases , *TOXIC psychoses , *FOOD poisoning , *SALMONELLA , *ANTIBACTERIAL agents - Abstract
The human pathogen Salmonella typhimurium can colonize, proliferate and persist in the intestine causing enteritis in mammals and mortality in the nematode Caenorhabditis elegans. Using C. elegans as a model, we determined that the Salmonella pathogenicity islands-1 and -2 (SPI-1 and SPI-2), PhoP and the virulence plasmid are required for the establishment of a persistent infection. We observed that the PhoP regulon, SPI-1, SPI-2 and spvR are induced in C. elegans and isogenic strains lacking these virulence factors exhibited significant defects in the ability to persist in the worm intestine. Salmonella infection also leads to induction of two C. elegans antimicrobial genes, abf-2 and spp-1, which act to limit bacterial proliferation. The SPI-2, phoP and Δ pSLT mutants are more sensitive to the cationic peptide polymyxin B, suggesting that resistance to worm's antimicrobial peptides might be necessary for Salmonella to persist in the C. elegans intestine. Importantly, we showed that the persistence defects of the SPI-2, phoP and Δ pSLT mutants could be rescued in vivo when expression of C. elegans spp-1 was reduced by RNAi. Together, our data suggest that resistance to host antimicrobials in the intestinal lumen is a key mechanism for Salmonella persistence. [ABSTRACT FROM AUTHOR]
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- 2008
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21. Characterization of mediators of microbial virulence and innate immunity using the Caenorhabditis elegans host–pathogen model.
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Alegado, Rosanna A., Campbell, Marianne C., Chen, Will C., Slutz, Sandra S., and Tan, Man-Wah
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CAENORHABDITIS elegans , *MICROBIAL virulence , *NATURAL immunity , *PATHOGENIC microorganisms - Abstract
Summary The soil-borne nematode, Caenorhabditis elegans , is emerging as a versatile model in which to study host–pathogen interactions. The worm model has shown to be particularly effective in elucidating both microbial and animal genes involved in toxin-mediated killing. In addition, recent work on worm infection by a variety of bacterial pathogens has shown that a number of virulence regulatory genes mediate worm susceptibility. Many of these regulatory genes, including the PhoP/Q two-component regulators in Salmonella and LasR in Pseudomonas aeruginosa , have also been implicated in mammalian models suggesting that findings in the worm model will be relevant to other systems. In keeping with this concept, experiments aimed at identifying host innate immunity genes have also implicated pathways that have been suggested to play a role in plants and animals, such as the p38 MAP kinase pathway. Despite rapid forward progress using this model, much work remains to be done including the design of more sensitive methods to find effector molecules and further characterization of the exact interaction between invading pathogens and C. elegans’ cellular components. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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22. Kū Hou Kuapā: Cultural Restoration Improves Water Budget and Water Quality Dynamics in Heʻeia Fishpond.
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Möhlenkamp, Paula, Beebe, Charles Kaiaka, McManus, Margaret A., Kawelo, Angela Hiʻilei, Kotubetey, Keliʻiahonui, Lopez-Guzman, Mirielle, Nelson, Craig E., and Alegado, Rosanna ʻAnolani
- Abstract
In Hawaiʻi, the transition from customary subsistence flooded taro agroecosystems, which regulate stream discharge rate trapping sediment and nutrients, to a plantation-style economy (c. the 1840s) led to nearshore sediment deposition—smothering coral reefs and destroying adjacent coastal fisheries and customary fishpond mariculture. To mitigate sediment transport, Rhizophora mangle was introduced in estuaries across Hawaiʻi (c. 1902) further altering fishpond ecosystems. Here, we examine the impact of cultural restoration between 2012–2018 at Heʻeia Fishpond, a 600–800-year-old walled fishpond. Fishpond water quality was assessed by calculating water exchange rates, residence times, salinity distribution, and abundance of microbial indicators prior to and after restoration. We hypothesized that R. mangle removal and concomitant reconstruction of sluice gates would increase mixing and decrease bacterial indicator abundance in the fishpond. We find that Heʻeia Fishpond's physical environment is primarily tidally driven; wind forcing and river water volume flux are secondary drivers. Post-restoration, two sluice gates in the northeastern region account for >80% of relative water volume flux in the fishpond. Increase in water volume flux exchange rates during spring and neap tide and shorter minimum water residence time corresponded with the reconstruction of a partially obstructed 56 m gap together with the installation of an additional sluice gate in the fishpond wall. Lower mean salinities post-restoration suggests that increased freshwater water volume influx due to R. mangle removal. Spatial distribution of microbial bio-indicator species was inversely correlated with salinity. Average abundance of Enterococcus and Bacteroidales did not significantly change after restoration efforts, however, average abundance of a biomarker specific to birds nesting in the mangroves decreased significantly after restoration. This study demonstrates the positive impact of biocultural restoration regimes on water volume flux into and out of the fishpond, as well as water quality parameters, encouraging the prospect of revitalizing this and other culturally and economically significant sites for sustainable aquaculture in the future. [ABSTRACT FROM AUTHOR]
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- 2019
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23. Spheres of Hope, Packets of Doom: the Good and Bad of Outer Membrane Vesicles in Interspecies and Ecological Dynamics.
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Lynch, Jonathan B. and Alegado, Rosanna A.
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- 2017
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24. Sex-dependent effects on gut microbiota regulate hepatic carcinogenic outcomes.
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Xie, Guoxiang, Wang, Xiaoning, Zhao, Aihua, Yan, Jingyu, Chen, Wenlian, Jiang, Runqiu, Ji, Junfang, Huang, Fengjie, Zhang, Yunjing, Lei, Sha, Ge, Kun, Zheng, Xiaojiao, Rajani, Cynthia, Alegado, Rosanna A., Liu, Jiajian, Liu, Ping, Nicholson, Jeremy, and Jia, Wei
- Abstract
Emerging evidence points to a strong association between sex and gut microbiota, bile acids (BAs), and gastrointestinal cancers. Here, we investigated the mechanistic link between microbiota and hepatocellular carcinogenesis using a streptozotocin-high fat diet (STZ-HFD) induced nonalcoholic steatohepatitis-hepatocellular carcinoma (NASH-HCC) murine model and compared results for both sexes. STZ-HFD feeding induced a much higher incidence of HCC in male mice with substantially increased intrahepatic retention of hydrophobic BAs and decreased hepatic expression of tumor-suppressive microRNAs. Metagenomic analysis showed differences in gut microbiota involved in BA metabolism between normal male and female mice, and such differences were amplified when mice of both sexes were exposed to STZ-HFD. Treating STZ-HFD male mice with 2% cholestyramine led to significant improvement of hepatic BA retention, tumor-suppressive microRNA expressions, microbial gut communities, and prevention of HCC. Additionally the sex-dependent differences in BA profiles in the murine model can be correlated to the differential BA profiles between men and women during the development of HCC. These results uncover distinct male and female profiles for gut microbiota, BAs, and microRNAs that may contribute to sex-based disparity in liver carcinogenesis, and suggest new possibilities for preventing and controlling human obesity-related gastrointestinal cancers that often exhibit sex differences. [ABSTRACT FROM AUTHOR]
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- 2017
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25. Complete Genome Sequence of Algoriphagus sp. PR1, Bacterial Prey of a Colony-Forming Choanoflagellate.
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Alegado, Rosanna A., Ferriera, Steven, Nusbaum, Chad, Young, Sarah K., Qian Zeng, Imamovic, Alma, Fairclough, Stephen R., and King, Nicole
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GENOMES , *GRAM-negative bacteria , *FLAGELLATA , *BIOLOGICAL evolution , *BACTERIA - Abstract
Bacteria are the primary food source of choanoflagellates, the closest known relatives of animals. Studying signaling interactions between the Gram-negative Bacteroidetes bacterium Algoriphagus sp. PRI and its predator, the choanoflagellate Salpingoeca rosetta, provides a promising avenue for testing hypotheses regarding the involvement of bacteria in animal evolution. Here we announce the complete genome sequence of Algoriphagus sp. PR1 and initial findings from its annotation. [ABSTRACT FROM AUTHOR]
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- 2011
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26. Collaborative research to inform adaptive comanagement: a framework for the He'eia National Estuarine Research Reserve.
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Winter, Kawika B., Rii, Yoshimi M., Reppun, Frederick A. W. L., Hintzen, Katy DeLaforgue, Alegado, Rosanna A., Bowen, Brian W., Bremer, Leah L., Coffman, Makena, Deenik, Jonathan L., Donahue, Megan J., Falinski, Kim A., Frank, Kiana, Franklin, Erik C., Kurashima, Natalie, Lincoln, Noa Kekuewa, Madin, Elizabeth M. P., McManus, Margaret A., Nelson, Craig E., Okano, Ryan, and Olegario, Anthony
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ESTUARINE reserves , *TRADITIONAL knowledge , *HUMAN behavior , *INDIGENOUS peoples , *LOCAL knowledge , *ECOSYSTEM services - Abstract
Globally, an increasing recognition of the importance of ecosystem-based management (EBM), Indigenous resource management (IRM), and Indigenous-led research and management is emerging; yet, case studies within scholarly literature illustrating comprehensive application of these theories and philosophies are scarce. We present the collaborative management model for the He'eia National Estuarine Research Reserve (NERR) as a contemporary Indigenous Community and Conserved Area (ICCA) that has synergistically operationalized these principles, as well as one that approaches research as a reciprocal collaboration with the Indigenous people and local community (IPLC) of place. The He'eia NERR was designated in 2017 through a process led by IPLC members in Hawai'i. This research framework is aimed at informing EBM within social-ecological systems. It, therefore, serves as an example of a program designed to demonstrate and provide practical solutions for adaptive resource management. The framework of the He'eia NERR embraces the values, perspectives, and IRM strategies that have been foundational for the people of the Pacific to thrive sustainably in the context of limited resources for millennia. As a program, the He'eia NERR aims to build bridges between coexisting worldviews as a means of informing policy in the realms of conservation and sustainability. We do this by weaving together conventional and Indigenous science to collaboratively develop research and collaboratively produce new knowledge. We examine these issues through the lens of holistic ecosystem services that consider both the reciprocal benefits that humans provide to nature as well as the full range of existential benefits that humans gain from nature. Research collaborations between the He'eia NERR and its partners (University of Hawai'i, state and federal agencies, and Indigenous-led NGOs operating in the community) are grounded in Indigenous and local knowledge (ILK) with applications that will guide a future of enhanced ecosystem services in a changing world. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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27. Ecomimicry in Indigenous resource management: optimizing ecosystem services to achieve resource abundance, with examples from Hawai‘i.
- Author
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Winter, Kawika B., Lincoln, Noa Kekuewa, Berkes, Fikret, Alegado, Rosanna A., Kurashima, Natalie, Frank, Kiana L., Pascua, Pua‘ala, Rii, Yoshimi M., Reppun, Frederick, Knapp, Ingrid S.S., McClatchey, Will C., Ticktin, Tamara, Smith, Celia, Franklin, Erik C., Oleson, Kirsten, Price, Melissa R., McManus, Margaret A., Donahue, Megan J., Rodgers, Kuulei S., and Bowen, Brian W.
- Subjects
- *
ECOSYSTEM management , *ECOSYSTEM services , *RESOURCE management , *POPULATION , *CULTURAL landscapes , *ECOLOGICAL resilience - Abstract
Here, we expand on the term "ecomimicry" to be an umbrella concept for an approach to adaptive ecosystem-based management of social-ecological systems that simultaneously optimizes multiple ecosystem services for the benefit of people and place. In this context, we define ecomimicry as a strategy for developing and managing cultural landscapes, built upon a deep understanding of the structure and function of ecosystems, that harnesses ecosystem processes for the purpose of balancing and sustaining key ecosystem services, rather than maximizing one service (e.g., food production) to the detriment of others. Ecomimicry arises through novel, place-based innovations or is adopted from elsewhere and adapted to local conditions. Similarly, precontact Hawaiian socialecological systems integrated a variety of ecomimicry schema to engender a complex system of adaptive resource management that enhanced biocultural diversity and supported resilient food systems, ultimately sustaining a thriving human population. In addition to presenting a synopsis of how ecomimicry was employed in the design and management of Hawaiian social-ecological systems, we identify and characterize specific ecomimicry applications. Within this context, we explore a revival of ecomimicry for biological conservation, biocultural restoration, resilience, and food security. We conclude with a discussion of how revitalizing such an approach in the restoration of social-ecological systems may address issues of conservation and sustainability in the Anthropocene. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
28. Systemic racial disparities in funding rates at the National Science Foundation.
- Author
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Chen CY, Kahanamoku SS, Tripati A, Alegado RA, Morris VR, Andrade K, and Hosbey J
- Subjects
- Systemic Racism, Engineering, Immunotherapy
- Abstract
Concerns about systemic racism at academic and research institutions have increased over the past decade. Here, we investigate data from the National Science Foundation (NSF), a major funder of research in the United States, and find evidence for pervasive racial disparities. In particular, white principal investigators (PIs) are consistently funded at higher rates than most non-white PIs. Funding rates for white PIs have also been increasing relative to annual overall rates with time. Moreover, disparities occur across all disciplinary directorates within the NSF and are greater for research proposals. The distributions of average external review scores also exhibit systematic offsets based on PI race. Similar patterns have been described in other research funding bodies, suggesting that racial disparities are widespread. The prevalence and persistence of these racial disparities in funding have cascading impacts that perpetuate a cumulative advantage to white PIs across all of science, technology, engineering, and mathematics., Competing Interests: CC Has previously applied for, received, and benefitted from funding through grants and fellowships awarded by the NSF; has previously served as an ad hoc reviewer for NSF proposal review, SK Has previously applied for, received, and is currently funded through grants and a fellowship awarded by the NSF, AT Has previously applied for, received, and/or is currently funded by grants from NSF; has also previously served as ad hoc and/or panel reviewers at NSF, RA, JH Has previously applied for, received, and/or is currently funded by grants from NSF; has previously served as ad hoc and/or panel reviewers at NSF, VM Has previously applied for, received, and/or is currently funded by grants from NSF; has previously served as ad hoc and/or panel reviewers at NSF. VRM currently serves on the Committee of Equal Opportunities in Science and Engineering to NSF, KA Has previously applied for and/or received grants from NSF
- Published
- 2022
- Full Text
- View/download PDF
29. Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate.
- Author
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Alegado RA, Grabenstatter JD, Zuzow R, Morris A, Huang SY, Summons RE, and King N
- Subjects
- Bacterial Typing Techniques, Bacteroidetes genetics, Bacteroidetes isolation & purification, Base Composition, Choanoflagellata, DNA, Bacterial genetics, Fatty Acids analysis, Geologic Sediments microbiology, Molecular Sequence Data, Phospholipids analysis, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Virginia, Bacteroidetes classification, Phylogeny, Seawater microbiology
- Abstract
A Gram-negative, non-motile, non-spore-forming bacterial strain, PR1(T), was isolated from a mud core sample containing colonial choanoflagellates near Hog Island, Virginia, USA. Strain PR1(T) grew optimally at 30 °C and with 3 % (w/v) NaCl. Strain PR1(T) contained MK-7 as the major menaquinone as well as carotenoids but lacked pigments of the flexirubin-type. The predominant fatty acids were iso-C(15 : 0) (29.4 %), iso-C(17 : 1)ω9c (18.5 %) and summed feature 3 (C(16 : 1)ω6c and/or C(16 : 1)ω7c; 11.3 %). The major polar lipids detected in strain PR1(T) were phosphatidylethanolamine, an unknown phospholipid, an aminophospholipid, an aminolipid and two lipids of unknown character. The DNA G+C content was 38.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PR1(T) fell within the cluster comprising the genus Algoriphagus and was most closely related to Algoriphagus halophilus JC 2051(T) (95.4 % sequence similarity) and Algoriphagus lutimaris S1-3(T) (95.3 % sequence similarity). The 16S rRNA gene sequence similarity between strain PR1(T) and the type strains of other species of the genus Algoriphagus were in the range 91-95 %. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain PR1(T) demonstrated that this strain was distinct from other members of the genus Algoriphagus, including its closest relative, A. halophilus. Based on phenotypic, chemotaxonomic, phylogenetic and genomic data, strain PR1(T) should be placed in the genus Algoriphagus as a representative of a novel species, for which the name Algoriphagus machipongonensis sp. nov. is proposed. The type strain is PR1(T) (= ATCC BAA-2233(T) = DSM 24695(T)).
- Published
- 2013
- Full Text
- View/download PDF
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