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Objective: To investigate whether the expression of Foxp3 in human's Papillary thyroid carcinoma (PTC) cells could be regulated by TLR4 signaling pathway activation. Methods: We used human's thyroid carcinoma K1 cells as the research object, and choose lipopolysaccharide (LPS) as a ligand to activate TLR4 signaling pathway. The mRNA expression level of Foxp3 was detected by RT-PCR method in different LPS concentrations (0, 5, 10, 20, 40 μg/mL) and at different time points (12, 24, 36, 48 h). The protein expression level of Foxp3 and TLR4 were detected by flow cytometry. The expression level of Foxp3 mRNA and protein were detected by RT-PCR and flow cytometry respectively after adding LPS inhibitor polymyxin B (PMB). Results: The mRNA level of Foxp3 was significantly increased by 10 μg/mL LPS at 24 hours (P<0.05). And the protein level of TLR4 was significantly increased by LPS activation. After the PMB blocking TLR4 signaling pathway, the expression of Foxp3 mRNA and protein were significantly decreased than that of without blocking group (P<0.05). Conclusions: In human's Papillary thyroid carcinoma (PTC) K1 cells, TLR4 as the upstream signaling molecules, it could be involved in regulation ofFoxp3 by its own expression changed. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of autocrine motility factor invasion and metastasis of hepatocellular carcinoma. Methods: Human liver LO2 cells and human liver cancer MHCC97-H cells were used as the research object, and the protein expression levels of AMF was tested. Chemically synthesized siRNA duplex targeting the AMF gene (AMF-siRNA) was transiently transfected into MHCC97-H cells protein expression levels of AMF was detected by Western blot; Cell proliferation was determined by MTT experiment tests after transfection. The effect of AMF knockdown on cell invasion and migration was analyzed by Transwell chamber assays; With cell suspension finally subcutaneously vaccinated mice and observe silence before and after the AMF genetic liver cell into a tumor ability. Results: The protein expression of AMF in MHCC97-H quantity was higher; Putting the small double-stranded SiRNA transfection into MHCC97-H, the protein expression of AMF significantly reduced (P＜0.05); After silence, AMF genetic sequences, MHCC97-H proliferation, migration and invasion forces were significantly decreased (P＜0.05); Using cell suspension subcutaneous inoculation of AMF gene in mice MHCC97-H formation of tumor size was smaller than the control group (P＜0.05). Conclusions: SiRNA-mediated silencing of AMF gene could significantly inhibit the cell invasion and metastasis of human hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]