1. Comparative analysis of epigenetic markers in plasma and tissue of patients with colorectal cancer.
- Author
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Kondratov, A. G., Nekrasov, K. A., Lototska, L. V., Panasenko, G. V., Stoliar, L. A., Lapska, Y. V., Kolesnyk, O. O., Shchepotin, I. B., Rynditch, A. V., and Kashuba^, V. I.
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COMPARATIVE studies , *EPIGENETICS , *COLON cancer patients , *METHYLATION , *DNA - Abstract
Aim. The work is devoted to the development of less invasive tools for the colorectal cancer (CRC) screening. Methods. Q-PCR and methylation-specific PCR techniques were used in the current work. Results. We have shown that the levels of cell-free plasma DNA are higher in the CRC patients compared with the healthy donors (p < 0.01). Hypermethylation of APC, FHIT, LRRC3B and HIC1 genes was studied in the tumor and plasma samples of CRC patients. Two-stage verification for CRC screening was proposed. Conclusions. We proposed and tested a novel approach for CRC screening based on the determination of cell-free DNA and methylated DNA fragments in the plasma. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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