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Vegetative propagation of Gigantochloa atter (Hassk.) Kurz. It is difficult due to their handling, transportation and poor root development. The tissue culture techniques allow increasing the number of these plants in a short period of time. In order to determine the effect of indole acetic acid (IAA) three concentrations (15, 20 and 25 mg l-1) on in vitro rooting were tested and a control without growth regulator it was included. After 20 days of culture the number of plants with roots was quantified, the plant height (cm) and root length (cm) was measured. The plants were transferred to acclimatization phase and after 20 days of culture survival was determined. The results showed that the IAA influenced the in vitro rooting of G. atter. By adding to the culture medium IAA (20.0 mg l-1) the highest percentages of rooting (96.0%) and ex vitro (60%) were obtained survival. [ABSTRACT FROM AUTHOR]

This study was aimed to achieve the morphological and anatomical characterization of in vitro plants of Bambusa vulgaris Schrad. exWendl during acclimatization. The morphological development of the leaf and root in plants acclimatized with 0 and 90 days after planting, as well as field plants with more than 15 years of planting were considered. Results showed important differences between the structures of the leaf in the evaluated periods. Both presented adaxial and abaxial monostratified epidermis, with presence of trichomes, stomata, micro-hairs in the intercostal area. The abaxial surface showed silica and papilla cells. In the analysis of the cross section of leaf blade B. vulgaris were observed bulliform and fusoid cells, confirming the presence of the xylem, phloem, epidermis, cuticle, vascular bundles, the chlorenchyma showing differences among the development stages. The longitudinal cut to the root-stem junction demonstrated a further development in plants acclimatized for 90 days. [ABSTRACT FROM AUTHOR]

Bambusa vulgaris var. vulgaris has several uses. The biotechnological propagation can be an alternative to reach the demand of planting material. This investigation was aimed to determine the influence of substrate type on the acclimatization of in vitro plants. Two main components (worm humus (HL) and zeolite (Z)) were used to produce the substrate. These were combined (HL 70% and 30% Z, HL 60% and 40% Z, HL 50% and Z 50%, HL 80% and 20% Z). Worm compost and zeolite substrate was used as control. In vitro plants from rooting phase were acclimatized for 90 days. The number of roots per plant was assessed every 10 days (root length, number of shoots per plant, plant height and leave number). Survival was determined after 30 days of planting. Plant fresh and dry mass (g) were determined, subsequently, after 90 days of culture. Results demonstrated that the type of substrate influenced on the acclimatization of plants of B. vulgaris in greenhouse. The highest survival (%) ,number and length of roots as well as number of shoots per plant, fresh and dry mass of roots and cauline area, were achieved when the zeolite and worm humus were combined and by increasing the proportion of this last product. [ABSTRACT FROM AUTHOR]