Search

Showing total 1 results
Start Over Search Limiters Full Text Topic beta-galactosidase Publication Type Academic Journals

Refine your results

1 results

1. Highly regioselective galactosylation of floxuridine catalyzed by beta-galactosidase from bovine liver.

Author

Zeng QM, Li N, and Zong MH

Subjects
Animals, Cattle, Enzyme Stability, Floxuridine chemistry, Hydrogen-Ion Concentration, Kinetics, Nitrophenylgalactosides metabolism, Prodrugs chemistry, Prodrugs metabolism, Substrate Specificity, Temperature, Time Factors, Floxuridine metabolism, Liver enzymology, beta-Galactosidase isolation & purification, beta-Galactosidase metabolism
Abstract

5'-O-beta-D-Galactosyl-floxuridine, a potential novel prodrug, was synthesized with a yield of 75% through beta-galactosidase-catalyzed transgalactosylation. This enzyme displayed absolute regioselectivity toward the 5'-position of floxuridine. For the reaction, the optimal conditions were pH 6.5 at 45 degrees C for 60 h with floxuridine to o-nitrophenyl-beta-D-galactoside at 2:1 (mol/mol). Under these conditions, the initial reaction rate and the maximum yield were 0.28 mM h(-1) and 75%, respectively.

Published
2010
Full Text
View/download PDF