17 results
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2. Highly conductive paper for energy-storage devices.
- Author
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Liangbing Hu, Jang Wook Choi, Yuan Yang, Sangmoo Jeong, La Mantia, Fabio, Li-Feng Cui, and Yi Cui
- Subjects
- *
CARBON nanotubes , *ENERGY storage equipment , *PAPER , *NANOWIRES , *SUPERCAPACITORS , *LITHIUM-ion batteries - Abstract
Paper, invented more than 2,000 years ago and widely used today in our everyday lives, is explored in this study as a platform for energy-storage devices by integration with 1D nanomaterials. Here, we show that commercially available paper can be made highly conductive with a sheet resistance as low as 1 ohm per square (π/sq) by using simple solution processes to achieve conformal coating of single-walled carbon nanotube (CNT) and silver nanowire films. Compared with plastics, paper substrates can dramatically improve film adhesion, greatly simplify the coating process, and significantly lower the cost. Supercapacitors based on CNT-conductive paper show excellent performance. When only CNT mass is considered, a specific capacitance of 200 F/g, a specific energy of 30-47 Watt-hour/kilogram (Wh/kg). a specific power of 200,000 W/kg, and a stable cycling life over 40,000 cycles are achieved. These values are much better than those of devices on other flat substrates, such as plastics. Even in a case in which the weight of all of the dead components is considered, a specific energy of 7.5 Wh/kg is achieved. In addition, this conductive paper can be used as an excellent lightweight current collector in lithium-ion batteries to replace the existing metallic counterparts. This work suggests that our conductive paper can be a highly scalable and low-cost solution for high-performance energy storage devices. [ABSTRACT FROM AUTHOR]
- Published
- 2009
3. Digital printing of shape-morphing natural materials.
- Author
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Zhao Z, Kumar J, Hwang Y, Deng J, Ibrahim MSB, Huang C, Suresh S, and Cho NJ
- Subjects
- Computer Simulation, Paper, Technology
- Abstract
We demonstrate how programmable shape evolution and deformation can be induced in plant-based natural materials through standard digital printing technologies. With nonallergenic pollen paper as the substrate material, we show how specific geometrical features and architectures can be custom designed through digital printing of patterns to modulate hygrophobicity, geometry, and complex shapes. These autonomously hygromorphing configurations can be "frozen" by postprocessing coatings to meet the needs of a wide spectrum of uses and applications. Through computational simulations involving the finite element method and accompanying experiments, we develop quantitative insights and a general framework for creating complex shapes in eco-friendly natural materials with potential sustainable applications for scalable manufacturing., Competing Interests: Competing interest statement: Z.Z., S.S., and N.-J.C. are listed as coinventors on a technology disclosure that was filed in relation to the findings of this study., (Copyright © 2021 the Author(s). Published by PNAS.)
- Published
- 2021
- Full Text
- View/download PDF
4. Pencil-paper on-skin electronics.
- Author
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Xu Y, Zhao G, Zhu L, Fei Q, Zhang Z, Chen Z, An F, Chen Y, Ling Y, Guo P, Ding S, Huang G, Chen PY, Cao Q, and Yan Z
- Subjects
- Electric Power Supplies, Electrodes, Equipment Design, Humans, Paper, Electronics instrumentation, Graphite, Monitoring, Physiologic instrumentation, Skin, Wearable Electronic Devices
- Abstract
Pencils and papers are ubiquitous in our society and have been widely used for writing and drawing, because they are easy to use, low-cost, widely accessible, and disposable. However, their applications in emerging skin-interfaced health monitoring and interventions are still not well explored. Herein, we report a variety of pencil-paper-based on-skin electronic devices, including biophysical (temperature, biopotential) sensors, sweat biochemical (pH, uric acid, glucose) sensors, thermal stimulators, and humidity energy harvesters. Among these devices, pencil-drawn graphite patterns (or combined with other compounds) serve as conductive traces and sensing electrodes, and office-copy papers work as flexible supporting substrates. The enabled devices can perform real-time, continuous, and high-fidelity monitoring of a range of vital biophysical and biochemical signals from human bodies, including skin temperatures, electrocardiograms, electromyograms, alpha, beta, and theta rhythms, instantaneous heart rates, respiratory rates, and sweat pH, uric acid, and glucose, as well as deliver programmed thermal stimulations. Notably, the qualities of recorded signals are comparable to those measured with conventional methods. Moreover, humidity energy harvesters are prepared by creating a gradient distribution of oxygen-containing groups on office-copy papers between pencil-drawn electrodes. One single-unit device (0.87 cm
2 ) can generate a sustained voltage of up to 480 mV for over 2 h from ambient humidity. Furthermore, a self-powered on-skin iontophoretic transdermal drug-delivery system is developed as an on-skin chemical intervention example. In addition, pencil-paper-based antennas, two-dimensional (2D) and three-dimensional (3D) circuits with light-emitting diodes (LEDs) and batteries, reconfigurable assembly and biodegradable electronics (based on water-soluble papers) are explored., Competing Interests: The authors declare no competing interest.- Published
- 2020
- Full Text
- View/download PDF
5. Paper-based microfluidics for DNA diagnostics of malaria in low resource underserved rural communities.
- Author
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Reboud J, Xu G, Garrett A, Adriko M, Yang Z, Tukahebwa EM, Rowell C, and Cooper JM
- Subjects
- Adolescent, Child, Humans, Malaria, Falciparum blood, Malaria, Falciparum parasitology, Polymerase Chain Reaction, Reproducibility of Results, DNA, Protozoan analysis, Health Resources, Malaria, Falciparum diagnosis, Medically Underserved Area, Microfluidics methods, Molecular Diagnostic Techniques methods, Paper, Rural Population
- Abstract
Rapid, low-cost, species-specific diagnosis, based upon DNA testing, is becoming important in the treatment of patients with infectious diseases. Here, we demonstrate an innovation that uses origami to enable multiplexed, sensitive assays that rival polymerase chain reactions (PCR) laboratory assays and provide high-quality, fast precision diagnostics for malaria. The paper-based microfluidic technology proposed here combines vertical flow sample-processing steps, including paper folding for whole-blood sample preparation, with an isothermal amplification and a lateral flow detection, incorporating a simple visualization system. Studies were performed in village schools in Uganda with individual diagnoses being completed in <50 min (faster than the standard laboratory-based PCR). The tests, which enabled the diagnosis of malaria species in patients from a finger prick of whole blood, were both highly sensitive and specific, detecting malaria in 98% of infected individuals in a double-blind first-in-human study. Our method was more sensitive than other field-based, benchmark techniques, including optical microscopy and industry standard rapid immunodiagnostic tests, both performed by experienced local healthcare teams (which detected malaria in 86% and 83% of cases, respectively). All assays were independently validated using a real-time double-blinded reference PCR assay. We not only demonstrate that advanced, low-cost DNA-based sensors can be implemented in underserved communities at the point of need but also highlight the challenges associated with developing and implementing new diagnostic technologies in the field, without access to laboratories or infrastructure., Competing Interests: The authors declare no conflict of interest., (Copyright © 2019 the Author(s). Published by PNAS.)
- Published
- 2019
- Full Text
- View/download PDF
6. Fluid-driven origami-inspired artificial muscles.
- Author
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Li S, Vogt DM, Rus D, and Wood RJ
- Subjects
- Animals, Artificial Limbs, Biomechanical Phenomena, Biomimetics economics, Biomimetics methods, Humans, Hydrodynamics, Robotics economics, Muscle, Skeletal physiology, Paper, Robotics methods
- Abstract
Artificial muscles hold promise for safe and powerful actuation for myriad common machines and robots. However, the design, fabrication, and implementation of artificial muscles are often limited by their material costs, operating principle, scalability, and single-degree-of-freedom contractile actuation motions. Here we propose an architecture for fluid-driven origami-inspired artificial muscles. This concept requires only a compressible skeleton, a flexible skin, and a fluid medium. A mechanical model is developed to explain the interaction of the three components. A fabrication method is introduced to rapidly manufacture low-cost artificial muscles using various materials and at multiple scales. The artificial muscles can be programed to achieve multiaxial motions including contraction, bending, and torsion. These motions can be aggregated into systems with multiple degrees of freedom, which are able to produce controllable motions at different rates. Our artificial muscles can be driven by fluids at negative pressures (relative to ambient). This feature makes actuation safer than most other fluidic artificial muscles that operate with positive pressures. Experiments reveal that these muscles can contract over 90% of their initial lengths, generate stresses of ∼600 kPa, and produce peak power densities over 2 kW/kg-all equal to, or in excess of, natural muscle. This architecture for artificial muscles opens the door to rapid design and low-cost fabrication of actuation systems for numerous applications at multiple scales, ranging from miniature medical devices to wearable robotic exoskeletons to large deployable structures for space exploration., Competing Interests: The authors declare no conflict of interest., (Copyright © 2017 the Author(s). Published by PNAS.)
- Published
- 2017
- Full Text
- View/download PDF
7. Paper-based plasma sanitizers.
- Author
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Xie J, Chen Q, Suresh P, Roy S, White JF, and Mazzeo AD
- Subjects
- Porosity, Disinfection methods, Escherichia coli growth & development, Paper, Plasma Gases chemistry, Saccharomyces cerevisiae growth & development
- Abstract
This work describes disposable plasma generators made from metallized paper. The fabricated plasma generators with layered and patterned sheets of paper provide a simple and flexible format for dielectric barrier discharge to create atmospheric plasma without an applied vacuum. The porosity of paper allows gas to permeate its bulk volume and fuel plasma, while plasma-induced forced convection cools the substrate. When electrically driven with oscillating peak-to-peak potentials of ±1 to ±10 kV, the paper-based devices produced both volume and surface plasmas capable of killing microbes. The plasma sanitizers deactivated greater than 99% of Saccharomyces cerevisiae and greater than 99.9% of Escherichia coli cells with 30 s of noncontact treatment. Characterization of plasma generated from the sanitizers revealed a detectable level of UV-C (1.9 nW⋅cm
-2 ⋅nm-1 ), modest surface temperature (60 °C with 60 s of activation), and a high level of ozone (13 ppm with 60 s of activation). These results deliver insights into the mechanisms and suitability of paper-based substrates for active antimicrobial sanitization with scalable, flexible sheets. In addition, this work shows how paper-based generators are conformable to curved surfaces, appropriate for kirigami-like "stretchy" structures, compatible with user interfaces, and suitable for sanitization of microbes aerosolized onto a surface. In general, these disposable plasma generators represent progress toward biodegradable devices based on flexible renewable materials, which may impact the future design of protective garments, skin-like sensors for robots or prosthetics, and user interfaces in contaminated environments., Competing Interests: Conflict of interest statement: US Provisional Patent was filed (62/291,082) for “Low-Cost, Flexible, Paper-Based Plasma Sterilizer” on February 4, 2016. US Patent Application was filed (15/425,474) for “Flexible Plasma Applicators Based on Fibrous Layers” on February 6, 2017.- Published
- 2017
- Full Text
- View/download PDF
8. Hydrogel-laden paper scaffold system for origami-based tissue engineering.
- Author
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Kim SH, Lee HR, Yu SJ, Han ME, Lee DY, Kim SY, Ahn HJ, Han MJ, Lee TI, Kim TS, Kwon SK, Im SG, and Hwang NS
- Subjects
- Alginates chemistry, Animals, Cartilage drug effects, Cartilage physiology, Chondrocytes cytology, Chondrocytes drug effects, Chondrocytes transplantation, Compressive Strength, Glucuronic Acid chemistry, HeLa Cells, Hexuronic Acids chemistry, Humans, Maleates chemistry, Mice, Inbred BALB C, Mice, Nude, Microscopy, Electron, Scanning, Molecular Weight, Neovascularization, Physiologic drug effects, Polystyrenes chemistry, Rabbits, Spectrometry, X-Ray Emission, Trachea drug effects, Trachea physiology, Hydrogel, Polyethylene Glycol Dimethacrylate pharmacology, Paper, Tissue Engineering methods, Tissue Scaffolds chemistry
- Abstract
In this study, we present a method for assembling biofunctionalized paper into a multiform structured scaffold system for reliable tissue regeneration using an origami-based approach. The surface of a paper was conformally modified with a poly(styrene-co-maleic anhydride) layer via initiated chemical vapor deposition followed by the immobilization of poly-l-lysine (PLL) and deposition of Ca(2+). This procedure ensures the formation of alginate hydrogel on the paper due to Ca(2+) diffusion. Furthermore, strong adhesion of the alginate hydrogel on the paper onto the paper substrate was achieved due to an electrostatic interaction between the alginate and PLL. The developed scaffold system was versatile and allowed area-selective cell seeding. Also, the hydrogel-laden paper could be folded freely into 3D tissue-like structures using a simple origami-based method. The cylindrically constructed paper scaffold system with chondrocytes was applied into a three-ring defect trachea in rabbits. The transplanted engineered tissues replaced the native trachea without stenosis after 4 wks. As for the custom-built scaffold system, the hydrogel-laden paper system will provide a robust and facile method for the formation of tissues mimicking native tissue constructs.
- Published
- 2015
- Full Text
- View/download PDF
9. Direct transfer of graphene onto flexible substrates.
- Author
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Martins LG, Song Y, Zeng T, Dresselhaus MS, Kong J, and Araujo PT
- Subjects
- Cellulose analogs & derivatives, Collodion, Hydrophobic and Hydrophilic Interactions, Paper, Paraffin, Polycarboxylate Cement, Polyethylene Terephthalates, Polymethyl Methacrylate, Polytetrafluoroethylene, Polyvinyl Chloride, Electronics trends, Graphite chemistry, Surface Properties
- Abstract
In this paper we explore the direct transfer via lamination of chemical vapor deposition graphene onto different flexible substrates. The transfer method investigated here is fast, simple, and does not require an intermediate transfer membrane, such as polymethylmethacrylate, which needs to be removed afterward. Various substrates of general interest in research and industry were studied in this work, including polytetrafluoroethylene filter membranes, PVC, cellulose nitrate/cellulose acetate filter membranes, polycarbonate, paraffin, polyethylene terephthalate, paper, and cloth. By comparing the properties of these substrates, two critical factors to ensure a successful transfer on bare substrates were identified: the substrate's hydrophobicity and good contact between the substrate and graphene. For substrates that do not satisfy those requirements, polymethylmethacrylate can be used as a surface modifier or glue to ensure successful transfer. Our results can be applied to facilitate current processes and open up directions for applications of chemical vapor deposition graphene on flexible substrates. A broad range of applications can be envisioned, including fabrication of graphene devices for opto/organic electronics, graphene membranes for gas/liquid separation, and ubiquitous electronics with graphene.
- Published
- 2013
- Full Text
- View/download PDF
10. Rapid prototyping of carbon-based chemiresistive gas sensors on paper.
- Author
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Mirica KA, Azzarelli JM, Weis JG, Schnorr JM, and Swager TM
- Subjects
- Microscopy, Electron, Scanning, Principal Component Analysis, Gases chemistry, Nanotubes, Carbon chemistry, Paper
- Abstract
Chemically functionalized carbon nanotubes (CNTs) are promising materials for sensing of gases and volatile organic compounds. However, the poor solubility of carbon nanotubes hinders their chemical functionalization and the subsequent integration of these materials into devices. This manuscript describes a solvent-free procedure for rapid prototyping of selective chemiresistors from CNTs and graphite on the surface of paper. This procedure enables fabrication of functional gas sensors from commercially available starting materials in less than 15 min. The first step of this procedure involves the generation of solid composites of CNTs or graphite with small molecule selectors--designed to interact with specific classes of gaseous analytes--by solvent-free mechanical mixing in a ball mill and subsequent compression. The second step involves deposition of chemiresistive sensors by mechanical abrasion of these solid composites onto the surface of paper. Parallel fabrication of multiple chemiresistors from diverse composites rapidly generates cross-reactive arrays capable of sensing and differentiating gases and volatile organic compounds at part-per-million and part-per-thousand concentrations.
- Published
- 2013
- Full Text
- View/download PDF
11. Paper-supported 3D cell culture for tissue-based bioassays.
- Author
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Derda R, Laromaine A, Mammoto A, Tang SK, Mammoto T, Ingber DE, and Whitesides GM
- Subjects
- Animals, Cell Line, Cell Separation, Cell Survival drug effects, Gene Expression Regulation drug effects, Humans, Hydrogels pharmacology, Mice, Oxygen pharmacology, Permeability drug effects, Biological Assay methods, Cell Culture Techniques methods, Paper
- Abstract
Fundamental investigations of human biology, and the development of therapeutics, commonly rely on 2D cell-culture systems that do not accurately recapitulate the structure, function, or physiology of living tissues. Systems for 3D cultures exist but do not replicate the spatial distributions of oxygen, metabolites, and signaling molecules found in tissues. Microfabrication can create architecturally complex scaffolds for 3D cell cultures that circumvent some of these limitations; unfortunately, these approaches require instrumentation not commonly available in biology laboratories. Here we report that stacking and destacking layers of paper impregnated with suspensions of cells in extracellular matrix hydrogel makes it possible to control oxygen and nutrient gradients in 3D and to analyze molecular and genetic responses. Stacking assembles the "tissue", whereas destacking disassembles it, and allows its analysis. Breast cancer cells cultured within stacks of layered paper recapitulate behaviors observed both in 3D tumor spheroids in vitro and in tumors in vivo: Proliferating cells in the stacks localize in an outer layer a few hundreds of microns thick, and growth-arrested, apoptotic, and necrotic cells concentrate in the hypoxic core where hypoxia-sensitive genes are overexpressed. Altering gas permeability at the ends of stacks controlled the gradient in the concentration of the O(2) and was sufficient by itself to determine the distribution of viable cells in 3D. Cell cultures in stacked, paper-supported gels offer a uniquely flexible approach to study cell responses to 3D molecular gradients and to mimic tissue- and organ-level functions.
- Published
- 2009
- Full Text
- View/download PDF
12. Three-dimensional microfluidic devices fabricated in layered paper and tape.
- Author
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Martinez AW, Phillips ST, and Whitesides GM
- Subjects
- Environmental Monitoring methods, Water chemistry, Microfluidic Analytical Techniques methods, Paper
- Abstract
This article describes a method for fabricating 3D microfluidic devices by stacking layers of patterned paper and double-sided adhesive tape. Paper-based 3D microfluidic devices have capabilities in microfluidics that are difficult to achieve using conventional open-channel microsystems made from glass or polymers. In particular, 3D paper-based devices wick fluids and distribute microliter volumes of samples from single inlet points into arrays of detection zones (with numbers up to thousands). This capability makes it possible to carry out a range of new analytical protocols simply and inexpensively (all on a piece of paper) without external pumps. We demonstrate a prototype 3D device that tests 4 different samples for up to 4 different analytes and displays the results of the assays in a side-by-side configuration for easy comparison. Three-dimensional paper-based microfluidic devices are especially appropriate for use in distributed healthcare in the developing world and in environmental monitoring and water analysis.
- Published
- 2008
- Full Text
- View/download PDF
13. Flexible energy storage devices based on nanocomposite paper.
- Author
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Pushparaj VL, Shaijumon MM, Kumar A, Murugesan S, Ci L, Vajtai R, Linhardt RJ, Nalamasu O, and Ajayan PM
- Subjects
- Electrochemistry instrumentation, Electrochemistry methods, Nanocomposites chemistry, Nanocomposites ultrastructure, Paper
- Abstract
There is strong recent interest in ultrathin, flexible, safe energy storage devices to meet the various design and power needs of modern gadgets. To build such fully flexible and robust electrochemical devices, multiple components with specific electrochemical and interfacial properties need to be integrated into single units. Here we show that these basic components, the electrode, separator, and electrolyte, can all be integrated into single contiguous nanocomposite units that can serve as building blocks for a variety of thin mechanically flexible energy storage devices. Nanoporous cellulose paper embedded with aligned carbon nanotube electrode and electrolyte constitutes the basic unit. The units are used to build various flexible supercapacitor, battery, hybrid, and dual-storage battery-in-supercapacitor devices. The thin freestanding nanocomposite paper devices offer complete mechanical flexibility during operation. The supercapacitors operate with electrolytes including aqueous solvents, room temperature ionic liquids, and bioelectrolytes and over record temperature ranges. These easy-to-assemble integrated nanocomposite energy-storage systems could provide unprecedented design ingenuity for a variety of devices operating over a wide range of temperature and environmental conditions.
- Published
- 2007
- Full Text
- View/download PDF
14. Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper.
- Author
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Sano H, Royer HD, and Sager R
- Subjects
- 5-Methylcytosine, Animals, Autoradiography, Bacteriophage phi X 174 analysis, Base Composition, Cattle, Chlamydomonas analysis, Chlamydomonas growth & development, Chloroplasts analysis, Collodion, Cricetinae, Cricetulus, Cytosine analysis, DNA Restriction Enzymes, DNA, Satellite analysis, DNA, Viral analysis, Diazonium Compounds, Escherichia coli analysis, Immunosorbent Techniques, Paper, Rabbits, Thymus Gland analysis, Cytosine analogs & derivatives, DNA analysis
- Abstract
A method to identify 5-methylcytosine (m5Cyt) in DNA immobilized on nitrocellulose paper by using antibody against m5Cyt raised in rabbits is described. Immobilized restriction fragments of DNA are incubated first with purified antibody against m5Cyt and then with goat anti-rabbit IgG labeled with 125I. Restriction fragments containing m5Cyt are visualized by autoradiography. By using this method, a heavily methylated fragment of about 1700 base pairs was identified in nuclear DNA fom Chinese hamster cells, the methylation pattern of calf thymus satellite I DNA was examined, and chloroplast DNAs that were extracted from various stage of the Chlamydomonas life cycle were compared. Little if any methylation was detected in chloroplast DNA from vegetative cells or from male gametes, whereas homologous DNAs from female gametes and from zygotes were heavily methylated. The sensitivity of the method was examined with calf thymus satellite I DNA (which contains approximately 40 m5Cyt residues per repeat unit of 1400 base pairs) and with phi X174 virion DNA (which contains a single m5Cyt per molecule). The presence of m5Cyt was detected with as little as 40 ng of phi X174 DNA containing 0.02 pmol of m5Cyt and with 100 ng of satellite DNA containing about 0.5 pmol of m5Cyt. Thus, the method makes possible the identification of individual methylated sites in purified DNAs in the size range of single genes.
- Published
- 1980
- Full Text
- View/download PDF
15. Immunoautoradiographic detection of proteins after electrophoretic transfer from gels to diazo-paper: analysis of adenovirus encoded proteins.
- Author
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Symington J, Green M, and Brackmann K
- Subjects
- Adenoviruses, Human analysis, Diazonium Compounds, Electrophoresis, Polyacrylamide Gel, Paper, Viral Proteins analysis, Autoradiography methods, Immunologic Techniques, Proteins analysis
- Abstract
We describe a method by which complex protein mixtures are fractionated by standard one-dimensional Na-DodSO4/polyacrylamide gel electrophoresis or O'Farrell two-dimensional gel electrophoresis and then are efficiently and rapidly transferred electrophoretically to diazobenzyloxymethyl- or diazophenylthioether-paper and analyzed by immunoautoradiography. The method is illustrated with protein extracts of human KB cells infected with adenovirus type 2. Proteins were transferred from gels without decrease in resolution and with an increase in the sensitivity of detection by autoradiography when [35S]-methionine-labeled proteins were used. When unlabeled proteins were transferred, low levels of virus encoded proteins could be detected by sequential treatment of diazobenzyloxymethyl-paper with anti-adenovirus type 2 virion or anti-73,000 DNA binding protein and 125I-labeled Staphyloccus aureus protein A. Covalently bound viral proteins retained immunologic reactivity after dissociation of the protein A and antibody, By one-dimensional gel transfer/immunoautoradiography, seven virion proteins were detected as prominent bands and several others as weaker bands. By two-dimensional gel transfer/immunoautoradiography, several additional viral proteins were detected. By use of anti-DNA binding protein serum, the Mr 73,000 protein and Mr 41,000-48,000 subspecies were detected. A protein present at a concentration of approximately 1 part in 100,000 of the total protein can be identified in cell extracts. This method may be applicable to various biological problems requiring resolution and detection of small amounts of specific proteins that can be recognized immunologically or that can be detected by binding to specific radiolabeled DNA or RNA sequences or hormones.
- Published
- 1981
- Full Text
- View/download PDF
16. Antibodies reactive with native lysozyme elicited by a completely synthetic antigen.
- Author
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Arnon R, Maron E, Sela M, and Anfinsen CB
- Subjects
- Alanine, Amino Acids analysis, Animals, Antibodies analysis, Antibodies isolation & purification, Bacteriophages immunology, Carbon Isotopes, Chemical Phenomena, Chemistry, Cross Reactions, Cysteine, Electrophoresis, Energy Transfer, Fluorometry, Goats, Iodine Isotopes, Molecular Weight, Muramidase pharmacology, Naphthalenes, Oxidation-Reduction, Paper, Peptides analysis, Peptides pharmacology, Polysaccharides, Rabbits, Sulfonic Acids, Trypsin, Ultracentrifugation, Antigen-Antibody Reactions, Muramidase chemical synthesis, Peptides chemical synthesis
- Abstract
A synthetic peptide consisting of the aminoacid sequence of residues 64-82 of lysozyme, with alanine replacing cysteine as residue 76, was prepared by the solid-phase technique. Mild reduction followed by reoxidation in air of the deprotected peptide led to the formation of a closed loop containing an intrachain disulfide bond. A conjugate consisting of this "loop" attached to multi-poly(DL-alanyl)-poly(L-lysine) elicited, in rabbits and goats, the formation of antibodies capable of reacting with lysozyme and with the loop peptide prepared from it. These immunological interactions can be inhibited by either lysozyme or the loop peptide, but not by the performic acid-oxidized open-chain peptide. Thus, the antibodies elicited by the completely synthetic antigen show specificity toward the "loop" structure (residues 64-80) of native lysozyme.
- Published
- 1971
- Full Text
- View/download PDF
17. Juvenile hormone activity for the bug Pyrrhocoris apterus.
- Author
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Sláma K and Williams CM
- Subjects
- Animals, Biological Assay, Metamorphosis, Biological, Hormones, Insecta, Paper
- Published
- 1965
- Full Text
- View/download PDF
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