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This instalment in our series on ultrahigh-pressure liquid chromatography (UHPLC) highlights its benefits in fast analysis, high-resolution separations, high performance liquid chromatography (HPLC) method development, reduced solvent and sample usage, and enhanced sensitivity and precision performance. [ABSTRACT FROM AUTHOR]

Dual flow chromatography (DFC) separations are performed with back and forth flow for rapid method development, design of experiments (DOE), quality-by-design (QbD), or high-throughput chromatographic purification. Although different than conventional unidirectional flow through chromatography, chromatographic principles still control the separations. Selectivity coefficients and Langmuir adsorption isotherms control the separation chemistry properties of the column and dictate the mobile phase conditions needed to achieve separation. However, the kinetic rates of diffusion and interaction of mobile phase molecules with the stationary phase, column channeling, and other column properties are not germane to the practice of DFC. Chromatographic conditions developed with DFC can be scaled to any size, including laboratory and industrial preparative columns. One practical use of DFC is in the pharmaceutical industry where multidimensional method development and high-throughput, parallel processing has been used to improve R&D productivity and decrease the time to manufacturing of biopharmaceutical drugs. [ABSTRACT FROM AUTHOR]

In the last five years, the acceptance and implementation of packed-column supercritical fluid chromatography-mass spectrometry (pSFC-MS) to drug discovery applications has gained momentum. This article describes the pros and cons of pSFC-MS and attempts to demonstrate its broad applicability to such fields as high-throughput analysis, purity assessment structure characterization and purification. Finally, an outlook for the future of this technique is presented. [ABSTRACT FROM AUTHOR]

The article focuses on the use of Kromasil CelluCoat in the analysis and purification of chiral compounds. Kromasil CelluCoat refers to a silica based chiral stationary phases (CSP) coated with tris-carbamoyl cellulose as the chiral selector. The analysis involves a factor of selectivity that leads to enantiomer separation, which leads to the speed of separation.

The article present information about introduction of new polymeric reversed-phase columns, Amberchrom™ Profile™. These columns have been developed for the purification of peptides and oligonucleotides by reversed-phase chromatography. The columns have been able to achieve the purification target yield of eighty percent at more than ninety five percent purity in the purification of influenza peptide, that is used in the area of vaccine development..

Describes the use of the multiOPLC system, an Optimum Performance Laminar Chromatography system that incorporates the Flowing Eluent Wall Technology, to perform simultaneous semi-preparative scale separations of complex samples. Reagents and separation media; Instrumentation; Capability of the system to produce four independent channels on an unsegmented flat-bed column.

Demonstrates the utility of Atlantis dC[sub 18] columns via the separation and purification of three mixtures of polar compounds. Experimental conditions; Description of Atlantis dC[sub 18] reversed-phase columns; Retention and separation of the sulphonamides on the analytical column.