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Bamboos are unique in bringing together a wide range of environmental services. The present work was conducted to determine the effect of immersion frequency of shoot multiplication of B. vulgaris grown in SIT. Three Immersion frequencies (every 4, 6 and 12 hours) were studied. The plant height was measured and the number of shoots per explant and the number of expanded leaves per explant were quantified. Furthermore, the water content was determined in the explants. It was found that the immersion frequency influenced the in vitro multiplication of B. vulgaris. The results showed that the number of shoots (6.5 shoots/explant) and the number of leaves per explant (11.0 leaves / explant) were higher with immersion every six hours. The lowest values for all variables were found in explants cultured with immersion every 12 h. In this treatment water content in the explants was higher. The results indicated that with the management of the immersion frequency it can encourage the multiplication of B. vulgaris in SIT. [ABSTRACT FROM AUTHOR]

Bambusa vulgaris Schrad. Ex Wendl is a species of great environmental importance and plays a vital role in the economic development of Cuba. The present work was carried out with objective of determining effect of inoculum density on morphology and physiology of the shoots of B. vulgaris cultivated in SIT. Morphological and physiological parameters of cultivated plants were evaluated at different inoculum densities (6, 12 and 18 explants per SIT) for 30 days. The results showed that densities studied had an effect on growth and physiology of shoots during in vitro multiplication. Studies revealed that number of shoots, shoot length, leaf numbers and chlorophyll increased with inoculum density of 12 explants. However, experiment showed that increase in inoculum density decreased accumulation of total chlorophyll, dry mass content of the shoots and water content. These results showed a better morpho-physiological response of shoots cultivated with 12 explants. This study provides an initial understanding of response of B. vulgaris shoots to cultivation of in vitro conditions, which is important for increasing production of bamboo plants. [ABSTRACT FROM AUTHOR]