1. Self-Assembling Nanovaccine Confers Complete Protection Against Zika Virus Without Causing Antibody-Dependent Enhancement.
- Author
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Rong, Heng, Qi, Mi, Pan, Jingdi, Sun, Yuhan, Gao, Jiawang, Zhang, Xiaowei, Li, Wei, Zhang, Bo, Zhang, Xian-En, and Cui, Zongqiang
- Subjects
ZIKA virus ,ZIKA virus infections ,VACCINE effectiveness ,PROTEIN domains ,VACCINE development - Abstract
The Zika virus (ZIKV) epidemic poses a substantial threat to the public, and the development of safe and effective vaccines is a demanding challenge. In this study, we constructed a kind of self-assembling nanovaccine which confers complete protection against ZIKV infection. The ZIKV envelop protein domain III (zEDIII) was presented on recombinant human heavy chain ferritin (rHF) to form the zEDIII-rHF nanoparticle. Immunization of mice with zEDIII-rHF nanoparticle in the absence of an adjuvant induced robust humoral and cellular immune responses. zEDIII-rHF vaccination conferred complete protection against lethal infection with ZIKV and eliminated pathological symptoms in the brain. Importantly, the zEDIII-rHF nanovaccine induced immune response did not cross-react with dengue virus-2, overcoming the antibody-dependent enhancement (ADE) problem that is a safety concern for ZIKV vaccine development. Our constructed zEDIII-rHF nanovaccine, with superior protective performance and avoidance of ADE, provides an effective and safe vaccine candidate against ZIKV. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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