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In order to investigate the effects of high-pressure micro-mist system of ambient temperature-humidity on behavior of captive Himalayan red pandas (Ailurus fulgens), the digital hygrometer thermometers were used from August to September 2020 to record ambient temperature-humidity, at the same time, behavior of pandas were observed using instantaneous scanning method, to compare the difference when the micro-fog system was on (trial period) and off (control period) in Hangzhou Zoo. The results showed that the ambient temperature in the trial period was significantly lower than that in the control period at 09: 00 (p<0. 05), and extremely significantly lower than that in the control period at 09: 30—11: 00 and 14: 00—15: 00 (p<0. 01) . The ambient humidity in the trial period was significantly higher than that in the control period at 09: 30—10: 00 and 14: 00—14: 30 (p<0. 05), and extremely significantly higher than that in the control period at 10: 30— 11: 00 and 15: 00 (p<0. 01). The alter and active behaviors of Himalayan red pandas in the trial period were significantly lower than those in the control period (p<0. 05), and the inactive behavior was significantly higher than that in the control period (p<0. 01). In the active behavior, the fast-moving behavior of the experimental group was significantly higher than that of the control group (p<0. 05), and the slow-moving behavior of the trial period was extremely significantly lower than that of the control period (p<0. 01). In the inactive behaviors, the sleeping and resting behaviors of the trial period were significantly higher than those of the control period (p<0. 01). In the selection of vertical space, the utilization rate of middle space in the trial period was significantly higher than that in the control period (p<0. 01), and the utilization rate of lower space was significantly lower than that in the control period (p<0. 01). In the selection of plane area, the utilization rate of the middle area in the trial period was significantly higher than that in the control period (p<0. 01), the utilization rate of the back area was significantly lower than that of the control group (p<0. 05). High-pressure micro-mist system can significantly reduce the environmental temperature, improve the environment humidity, provide cool and moist external environment for red pandas. Red pandas showed more resting and fast-moving behaviors like playing or running, reduce the slow-moving and alter behaviors, appeared more relaxed. When the high-pressure micro-mist system was on, Himalayan red pandas were more likely to be found in the middle space, and were much more visible for exhibitions. [ABSTRACT FROM AUTHOR]