1. ARID1A suppresses malignant transformation of human pancreatic cells via mediating senescence-associated miR-503/CDKN2A regulatory axis.
- Author
-
Li, Zhao-yun, Zhu, Shan-shan, Chen, Xian-jun, Zhu, Jie, Chen, Qi, Zhang, Ya-qiong, Zhang, Chun-ling, Guo, Ting-ting, and Zhang, Li-ming
- Subjects
- *
PANCREATIC beta cells , *CELLULAR aging , *MICRORNA , *GENETIC mutation , *CHROMATIN-remodeling complexes , *NEOPLASTIC cell transformation - Abstract
ARID1A as a subunit of SWI/SNF chromatin complexes is frequently mutated in human pancreatic cancer, however its exact role in pancreatic tumorigenesis remain unclear. In this study, we investigated the effects of ARID1A loss on human pancreatic epithelial cell lines HPNE, BxPC-3 with KRAS mutant (KRAS G12D ) expression. We found that ARID1A knockdown promoted cell proliferation and colony formation in cooperation with active mutant KRAS G12D . Function assay revealed that ARID1A knockdown accelerated cell cycle progression, and repressed KRAS G12D -induced cell senescence. Transcriptome analysis revealed ARID1A knockdown led to miR-503 upregulation. CDKN2A was identified as a target of miR-503, which contributes to cell senescence. Thus, our data suggests that ARID1A deficiency promote KRAS G12D -driven pancreatic tumorigenesis through miR-503/CDKN2A-mediated senescence. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF