1. Polysaccharides from Balanophora harlandii Hook: Isolation, characterization, and anti-inflammation activities.
- Author
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Li Y, Li X, Yuan Q, Zhao J, Wu W, Gui Y, Wang H, Wang L, Luo Y, Zhou G, Zhang J, He Y, and Yuan C
- Subjects
- Mice, Animals, RAW 264.7 Cells, Molecular Weight, Balanophoraceae chemistry, Plant Extracts pharmacology, Plant Extracts chemistry, Plant Extracts isolation & purification, Macrophages drug effects, Macrophages metabolism, Lipopolysaccharides pharmacology, NF-kappa B metabolism, Tumor Necrosis Factor-alpha metabolism, Inflammation drug therapy, Polysaccharides pharmacology, Polysaccharides chemistry, Polysaccharides isolation & purification, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents isolation & purification
- Abstract
Balanophora harlandii Hook (B. harlandii), a folk medicine, has been traditionally employed to treat traumatic bleeding, gastroenteritis, icteric hepatitis, hemorrhoids, and other conditions. In this work, polysaccharides with anti-inflammatory effects were extracted from B. harlandii and purified. The extraction conditions were optimized, and the properties of one purified neutral fraction, denoted as BHPs-W-S3, were analyzed. BHPs-W-S3 has a molecular weight of 14.1 kDa, and its three main monosaccharides are glucose, galactose, and xylose, with a molar ratio of 6.4:1.7:1.1. Its main chain consists of →6)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, →6)-β-D-Galp-(1→, →3,6)-β-D-Galp-(1→, and it has branch chains at the O-4 and/or O-3 positions. In addition, in vitro experiments showed that the polysaccharides from B. harlandi can decrease the phosphorylation level of p65 and IκBα in LPS-induced RAW264.7 cells to reduce the expression of the pro-inflammatory genes such as TNF-α, IL-6, and IL-1β., Competing Interests: Declaration of Competing Interest The authors state that there are no conflicts of interest to disclose., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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