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The starch content of shoot-forming and non-shoot-forming tobacco callus cultured in light and darkness was determined. A variety of carbohydrates and cytokinins incorporated into the culture medium were effective in bringing about starch accumulation and shoot formation in the tissue. In addition, the respiratory activity of the callus, grown in the presence or absence of gibberellic acid, was measured. A strong correlation between the starch content of the tissue, its rate of respiration, and shoot formation was observed. [ABSTRACT FROM AUTHOR]

The diffusion coefficient of oxygen in stirred water in which the surface is unbroken is approximately 0.01 mg cm-2 min-1 atm-1. Under these conditions, the modified Barcroft respirometer would not give wlid results. Hence, it is recommended that this method be abandoned for studies of algal metabolism. [ABSTRACT FROM AUTHOR]