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Bambusa vulgaris var. vulgaris Schrad. ex Wendl. is a bamboo species of great economic importance. Developing protocols for propagation via organogenesis and somatic embryogenesis would be an efficient alternative to propagate this species since it adapts well to the soil and climatic conditions of Cuba. The aim of this work was to determine the effect of different concentrations of 6-BAP (1.0, 3.0 and 6.0 mg·l-1) and NAA (0, 0.5, 1.0 mg·l-1) on B. vulgaris in vitro multiplication. Number of shoots and leaves per explant were quantified. Also, height of plants was measured on the twentieth day of culture. Multiplication of Bambusa vulgaris var. vulgaris in liquid culture medium with BAP was achieved. Combination of BAP and NAA in the culture medium was not effective for this purpose. The result solves one of the main limitations to use biotechnology techniques on this crop. [ABSTRACT FROM AUTHOR]

The availability of D. strictus seeds is affected by the length and erratic flowering cycle of the species. These seeds also retain their germination only for short periods of time. The tissue culture offers a fast and reliable way for the propagation using in vitro seed germinated as initial plant material. This research was conducted with the objective of to germinate in vitro D. strictus seeds. The effect of different concentrations of sodium hypochlorite (NaClO) (3.0, 5.0 and 7.0%) and time for seed disinfection (20 and 30 min) were determined. It was evaluated the number of germinated seeds and free of microbial contaminants after 10 days of culture. The results showed that the method of disinfection which sodium hypochlorite 3.0% for 30 minutes was the best for in vitro germination of Dendrocalamus strictus (Rosb.) Nees seeds. [ABSTRACT FROM AUTHOR]