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The thermophilic fungus Gymnascella thermotolerans GTE-21 produces a significant amount of both volatile and nonvolatile compounds with potent antifungal and anticancer properties. Gas chromatography-mass spectrometry (GC-MS) was utilized to identify 14 volatile organic compounds (VOCs) released by G. thermotolerans. The most predominant compound identified was diisooctyl phthalate, constituting 71.99% of the total VOCs, followed by phenol, 2-methoxy-4-(2-propenyl) at 7.12%. The GTE-21's VOCs demonstrated significant antifungal growth inhibition activity of 42.5% against Fusarium solani and 39.03% against Alternaria alternata. Additionally, GTE-21's crude extract exhibited substantial antibacterial effects, evidenced by inhibition zones of 1.5 cm against S. epidermidis, 1.4 cm against C. ciferrii, and 1.3 cm against S. aureus. The CHR2RClR2R:MeOH (1:1) fraction was more effective against human pathogens, producing the largest zones of inhibition of 1.1 and 1.03 cm against S. epidermidis and C. ciferrii, respectively. The evaluation of GTE-21's antifungal activity using the Evans blue assay showed that fungal spores treated with GTE-21's crude extract exhibited 96.2% inhibition at a concentration of 25 mg/ml. Similarly, the fractions CH2Cl2:MeOH (1:1) and CH2Cl2:MeOH (9:1) demonstrated spore inhibition rates of 96.3% and 94.3%, respectively. The EtOAc crude extract demonstrated pronounced cytotoxic effects against the SKOV-3 and MDA-MB-231 cancer cell lines. For SKOV-3, cell viability decreased to 3.40% and 0.47% at concentrations of 50 and 100 μL, respectively. In the case of MDA-MB-231, cell viability was reduced to 0.19% at both 50 and 100 μL concentrations. [ABSTRACT FROM AUTHOR]

The okra crop, cultivated all over the globe, is a source of oil and protein. Despite the economic significance of okra in the livelihoods of millions of small-scale farmers, studies on the nutritional traits of okra are meager. Creating novel hybrid lines with desirable characteristics could be employed to maximize the nutritional quality of the okra plant, precisely the amount of anthocyanin content, phenols, and total antioxidants. We crossed an Egyptian variety, preferably in the market but with low production, with a highly productive Japanese variety with less nutritional value for consumers. The two okra genotypes with their hybrids and 16 segregating populations were arranged in a randomized complete block design. Approximate chemical analyses for primary and secondary metabolites were quantified for each genotype. The analysis of variances indicated a significant variation among okra genotypes in all the physiological and nutritional traits except for ascorbic acid content. Various traits revealed by Principal Component Analysis contributed distinctively to the total variation. Correlation analysis indicated some significant correlations between the nutritional characteristics. Protein contents had a positive and significant correlation with total antioxidant (0.521), 2,2-diphenyl-1-picrylhydrazyl (0.488), proline (0.498), flavonoids (0.474), and saponin (0.536). [ABSTRACT FROM AUTHOR]

Rot disease is responsible for serious economic losses related to okra (Abelmoschus esculentus) crops cultivated in Upper Egypt. Colonies with a consistent morphology were isolated from the infected okra stems and leaves and subjected to morphological and molecular examinations. The causal pathogen was identified as Cochliobolus spicifer based on morphological fungus descriptions, as well as on the amplified 28S rDNA and internal transcribed spacer region (ITS) sequences, which showed 99%–100% similarity to the sequences of C. spicifer - CSN-20 strains. The volatile and non-volatile organic compounds (VOCs and n-VOCs, respectively) produced by the endophytic fungi that are associated with the medicinal plant Solenostemma arghel , namely Fusarium solani -F4-1007, Penicillium verrucosum -F2-1006, and Aspergillus terreus -F5-1008, inhibited the growth of the C. spicifer pathogen by 34.2%,31.4%, and 30.5%, respectively. In total,27 VOCs were identified by GC/MS, among which eight were specific to A. terreus -F5-1008, eight to P. verrucosum -F2-1006, and three to F. solani -F4-1007, whereas nine VOCs were commonly produced by the three endophytic fungi. Moreover, F. solani -F4-1007-produced VOCs and n-VOCs exhibited the highest antifungal activity, with 37.27% and 37.1% inhibition against C. spicifer colony growth, respectively. The potent antifungal VOCs produced by F. solani -F4-1007 were identified as 3,4-dihydro-2 h-1,5-(3″-t-butyl) benzodioxepine, 4-(2-hydroxyethyl) phenol, and phenylethyl alcohol using GC/MS. Therefore, F. solani -F4-1007 was tested as a potential biocontrol agent against C. spicifer- CSN-20 using an in-planta assay. Okra plants treated with endophytic F. solani -F4-1007did not show any disease symptoms, whereas those that were not treated with F. solani -F4-1007 exhibited severe disease symptoms when challenged with inoculation of the C. spicifer pathogen. Our results demonstrated the contribution of the endophytic fungus F. solani -F4-1007 as a potential biocontrol agent against the C. spicifer pathogen, to improve okra growth. [ABSTRACT FROM AUTHOR]