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Anthocyanins are important health-promoting flavonoid compounds that substantially contribute to fruit quality. Anthocyanin biosynthesis and most regulatory mechanisms are relatively well understood. However, the functions of anthocyanin transport genes in strawberry fruit remain unclear. In this study, a gene encoding an ATP-binding cassette (ABC) protein of type C, ABCC8, was isolated from strawberry fruits. qRT-PCR analysis demonstrated that the transcript levels of FvABCC8 were the highest and were strongly correlated with anthocyanin accumulation during strawberry fruit ripening. Transient overexpression and RNAi of FvABCC8 led to an increase and decrease in anthocyanin content in strawberry fruits, respectively. Moreover, the ABCC8 promoter was activated by MYB and bHLH transcription factors MYB10, bHLH33, and MYC1. Sucrose enhanced anthocyanin accumulation in FvABCC8- overexpressing Arabidopsis , particularly at higher concentrations. FvABCC8 -overexpressing lines were less sensitive to ABA during seed germination and seedling development. These results suggest that strawberry vacuolar anthocyanin transport may be mediated by the ABCC transporter ABCC8, the expression of which may be regulated by transcription factors MYB10, bHLH33, and MYC1. • The expression of FvABCC8 was correlated with the anthocyanin content during the strawberry fruit ripening. • FvABCC8 transcription promoted anthocyanin accumulation. • FvABCC8 expression was regulated by FvMYB10, FvMYC1, and FvbHLH33, but not FvbHLH3. • FvABCC8- overexpression Arabidopsis displayed higher sucrose sensitivity and was less sensitive to ABA than the wild-type. [ABSTRACT FROM AUTHOR]