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Ground-based microwave radiometers (MWRPS) can provide continuous atmospheric temperature and relative humidity profiles for a weather prediction model. We investigated the impact of assimilation of ground-based microwave radiometers based on the rapid-refresh multiscale analysis and prediction system-short term (RMAPS-ST). In this study, five MWRP-retrieved profiles were assimilated for the precipitation enhancement that occurred in Beijing on 21 May 2020. To evaluate the influence of their assimilation, two experiments with and without the MWRPS assimilation were set. Compared to the control experiment, which only assimilated conventional observations and radar data, the MWRPS experiment, which assimilated conventional observations, the ground-based microwave radiometer profiles and the radar data, had a positive impact on the forecasts of the RMAPS-ST. The results show that in comparison with the control test, the MWRPS experiment reproduced the heat island phenomenon in the observation better. The MWRPS assimilation reduced the bias and RMSE of two-meter temperature and two-meter specific humidity forecasting in the 0–12 h of the forecast range. Furthermore, assimilating the MWRPS improved both the distribution and the intensity of the hourly rainfall forecast, as compared with that of the control experiment, with observations that predicted the process of the precipitation enhancement in the urban area of Beijing. [ABSTRACT FROM AUTHOR]