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Plants of Bambusa vulgaris Schrad. ex Wendl are a viable option to generate environmental, social and economic benefits. However, there are problems in the propagation via organogenesis of this species such as the low in vitro multiplication coefficient, which limits its propagation. The research was aimed to determine the effect of 6-benzylaminopurine and the immersion time on the multiplication, morphophysiology and biochemistry of B. vulgaris shoots in temporary immersion systems. Morphophysiological and biochemical variables were evaluated in shoots grown at different BAP concentrations (0; 6.6; 13.2 and 26.4) at immersion times of one, two or three minutes, for 30 days of cultivation. In those systems, the lower concentration of BAP (6.6 µM) and immersion time of two minutes favored the emission of new shoots. It was associated with an increase in the content of total chlorophylls, lignin and total phenols in shoots. Based on these results, a protocol was established for the propagation via organogenesis of B. vulgaris plants in Temporary Immersion Systems, with a high emission of new shoots in vitro, which can be applied to other bamboo species. [ABSTRACT FROM AUTHOR]

Vegetative propagation of Gigantochloa atter (Hassk.) Kurz. It is difficult due to their handling, transportation and poor root development. The tissue culture techniques allow increasing the number of these plants in a short period of time. In order to determine the effect of indole acetic acid (IAA) three concentrations (15, 20 and 25 mg l-1) on in vitro rooting were tested and a control without growth regulator it was included. After 20 days of culture the number of plants with roots was quantified, the plant height (cm) and root length (cm) was measured. The plants were transferred to acclimatization phase and after 20 days of culture survival was determined. The results showed that the IAA influenced the in vitro rooting of G. atter. By adding to the culture medium IAA (20.0 mg l-1) the highest percentages of rooting (96.0%) and ex vitro (60%) were obtained survival. [ABSTRACT FROM AUTHOR]

Bambusa vulgaris Schrad. Ex Wendl is a species of great environmental importance and plays a vital role in the economic development of Cuba. The present work was carried out with objective of determining effect of inoculum density on morphology and physiology of the shoots of B. vulgaris cultivated in SIT. Morphological and physiological parameters of cultivated plants were evaluated at different inoculum densities (6, 12 and 18 explants per SIT) for 30 days. The results showed that densities studied had an effect on growth and physiology of shoots during in vitro multiplication. Studies revealed that number of shoots, shoot length, leaf numbers and chlorophyll increased with inoculum density of 12 explants. However, experiment showed that increase in inoculum density decreased accumulation of total chlorophyll, dry mass content of the shoots and water content. These results showed a better morpho-physiological response of shoots cultivated with 12 explants. This study provides an initial understanding of response of B. vulgaris shoots to cultivation of in vitro conditions, which is important for increasing production of bamboo plants. [ABSTRACT FROM AUTHOR]