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Increasing amounts of crop residues are being returned to croplands. Understanding nitrogen (N) availability in crop residues under various N fertilization regimes is important in optimizing N management. Pot experiments were conducted to investigate the contribution, fate and residual effects of urea and maize residue-N using a 15N isotope technique. Four N regimes were tested: three basal–topdressing ratios of N applied as urea (100–0, 75–25, 50–50) and one basal application of 75% N as urea and 25% N as manure (75U+25M). An average of 31.4% wheat N uptake was derived from urea, 9.2% from maize residue, and 59.5% from the soil in the first season. During the growing stages of wheat, maize residue contributed 0.3–4.8% and 3.1–13.2% to soil mineral and microbial biomass N, respectively, and those originated from urea was 1.0–4.2% and 4.6–16.8%, respectively. Regarding the fate of urea and residue-N after the first season, 35.9% and 16.9% of urea-15N and residue-15N were recovered by wheat, 28.1% and 46.9% remained in the soil, and 36.0% and 36.2% were lost. The contribution of urea to crop N uptake and N recovery efficiency increased, while that of residue-N decreased with increasing proportion of topdressing N. Substituting 25% mineral N with manure decreased the urea-15N loss without negative effects on crop dry matter and N uptake. Residual urea-15N and maize residue-15N from the previous season contributed 3.9% and 3.0% to summer maize N uptake. Additionally, 29.3% of residue-15N remained in the soil after the second season, while only 18.6% of urea-15N remained. Our study suggests that fertilizer and crop residue are actively involved in soil N transformation and plant N nutrition, emphasizing the capacities of organic residues to sustainably supply nutrients. Considering the utilization of both N fertilizer and maize residue, we may suggest a 75–25 split in N fertilizer application, but more appropriate options need to be further assessed under different cropping systems. [ABSTRACT FROM AUTHOR]

There is a growing concern that greenhouse gas (GHG) emissions during agricultural energy crop production might negate GHG emission savings which was not intended when promoting the use of renewable energy. Nitrous oxide (NO) is a major GHG, and in addition, it is the most powerful ozone-depleting compound that is emitted by human activity. The use of N fertilizers and animal manures is the main anthropogenic source of NO emissions. In spite of their high relevance, we still have limited understanding of the complex underlying microbial processes that consume or produce NO and their interactions with soil types, fertilizers (rate and types), plants, and other environmental variables. In a 2-year field experiment, we compared two important biogas crops in two different agro-ecological regions of northern Germany for their productivity and GHG emissions, using the closed-chamber technique and high time-resolution sampling. Silage maize, which is currently the most widespread crop grown for biogas fermentation purposes in Germany, was compared with an alternative bioenergy crop at each site. The three forms of nitrogen fertilizers/manures were given: calcium ammonium nitrate, cattle/pig slurry, and biogas residue. The greatest NO flux activity occurred in the period of May-July in all crops and at both sites. Flux patterns indicated pronounced effects of soil moisture-soil mineral-N interactions which were also seen as causation of the higher NO fluxes in the bioenergy crop maize compared to the other tested energy crops. However, the NO emission per unit methane production (specific NO emission) was clearly lower in soils planted with maize due to significantly higher methane hectare yield of maize. Our data suggest a linear relationship between increasing N input and increases in NO emission in both years at site with sandy loam texture where highest NO fluxes were measured. At sandy loam site, the percentage of applied N being emitted as NO was 1.9 and 1.1 % in soils cropped with maize and 0.9 and 0.8 % in soils cropped with wheat during the investigation period 2007-2008 and 2008-2009, respectively. In contrast, at site with sandy soil texture, the percentage of applied N emitted as NO was only 0.6 and 0.7 % in maize soils and 0.4 and 0.3 % in grassland during 2007-2008 and 2008-2009 period, respectively. Higher daily and annual NO emissions at the sandy loam site were attributed to the finer soil texture and higher denitrification activity. The present study provides a very good basis for the assessment of direct emissions of greenhouse gases from relevant biogas crops in North-West Europe. [ABSTRACT FROM AUTHOR]