117 results on '"Kato, Taku"'
Search Results
2. Application of first-generation high- and low-dose drug-coated balloons to the femoropopliteal artery disease: a sub-analysis of the POPCORN registry
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Fujihara, Masahiko, Takahara, Mitsuyoshi, Soga, Yoshimitsu, Iida, Osamu, Kawasaki, Daizo, Tomoi, Yusuke, Tsubakimoto, Yoshinori, Ogata, Kenji, Karashima, Eiji, Kato, Taku, Kobayashi, Yohei, Kaneko, Nobuhito, Sasaki, Shinya, and Ichihashi, Kei
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- 2023
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3. Chromogenic in situ hybridization reveals specific expression pattern of long non-coding RNA DRAIC in formalin-fixed paraffin-embedded specimen
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Sakurai, Kouhei, Yamada, Seiji, Ito, Rika, Ochiai, Mako, Ando, Tatsuya, Sakai, Yasuhiro, Kato, Taku, and Ito, Hiroyasu
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- 2024
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4. Gene therapy of prostate cancer using liposomes containing perforin expression vector driven by the promoter of prostate-specific antigen gene
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Mizutani, Kosuke, Kawakami, Kyojiro, Fujita, Yasunori, Kato, Taku, Takai, Manabu, Kato, Daiki, Iinuma, Koji, Koie, Takuya, and Ito, Masafumi
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- 2022
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5. Influence of lifestyle choices on risks of CYP1B1 polymorphisms for prostate cancer
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Kato, Taku, Hashimoto, Yutaka, Wong, Ryan K, Mitsui, Yozo, Maekawa, Shigekatsu, Chang, Inik, Shahryari, Varahram, Yamamura, Soichiro, Majid, Shahana, Saini, Sharanjot, Tabatabai, Z Laura, Dahiya, Rajvir, Deguchi, Takashi, and Tanaka, Yuichiro
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Biochemistry and Cell Biology ,Medicinal and Biomolecular Chemistry ,Chemical Sciences ,Biological Sciences ,Urologic Diseases ,Human Genome ,Prevention ,Genetics ,Cancer ,Tobacco Smoke and Health ,Prostate Cancer ,Tobacco ,2.1 Biological and endogenous factors ,Aetiology ,Adult ,Aged ,Aged ,80 and over ,Alcohol Drinking ,Alleles ,Case-Control Studies ,Cell Line ,Tumor ,Cytochrome P-450 CYP1B1 ,Gene Expression ,Gene-Environment Interaction ,Haplotypes ,Humans ,Life Style ,Male ,Middle Aged ,Mutagenesis ,Site-Directed ,Polymorphism ,Genetic ,Prostate ,Prostatic Neoplasms ,Risk Factors ,Smoking ,White People ,alcohol consumption ,cytochrome P450 1B1 ,polymorphisms ,prostate cancer ,smoker ,Clinical Sciences ,Biochemistry & Molecular Biology ,Biochemistry and cell biology ,Medicinal and biomolecular chemistry - Abstract
Cytochrome P450 1B1 (CYP1B1) converts xenobiotics to carcinogens and how lifestyle choices may interact with CYP1B1 polymorphisms and affect prostate cancer risk was assessed. Blood genomic DNA from a Caucasian population was analysed at polymorphic sites of the 5' untranslated region of CYP1B1 using TaqMan genotyping assays. Overall, drinker status and minor alleles at rs2551188, rs2567206 and rs10175368 were associated with prostate cancer. Linkage was observed between rs2551188, rs2567206, rs2567207 and rs10175368, and the G-C-T-G haplotype (major allele at respective sites) was decreased in cancer. Interestingly when classified by lifestyle factors, no associations of genotypes were found for non-smokers and non-drinkers, whereas on the contrary, minor type at rs2567206 and rs10175368 increased and major G-C-T-G decreased risk for cancer among smokers and drinkers. Interestingly, rs2551188, rs2567206 and rs10175368 minor genotypes correlated with increased tissue CYP1B1 as determined by immunohistochemistry. Further, rs10175368 enhanced luciferase activity and mobility shift show stronger binding of nuclear factor for the minor allele. These results demonstrate smoking and alcohol consumption to modify the risks of CYP1B1 polymorphisms for prostate cancer which may be through rs10175368, and this is of importance in understanding their role in the pathogenesis and as a biomarker for this disease.
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- 2018
6. EPHA2 is a novel cell surface marker of OCT4-positive undifferentiated cells during the differentiation of mouse and human pluripotent stem cells.
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Intoh, Atsushi, Watanabe-Susaki, Kanako, Kato, Taku, Kiritani, Hibiki, and Kurisaki, Akira
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- 2024
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7. The role of miR-24 as a race related genetic factor in prostate cancer
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Hashimoto, Yutaka, Shiina, Marisa, Kato, Taku, Yamamura, Soichiro, Tanaka, Yuichiro, Majid, Shahana, Saini, Sharanjot, Shahryari, Varahram, Kulkarni, Priyanka, Desgupta, Pritha, Mitsui, Yozo, Sumida, Mitsuho, Deng, Guoren, Tabatabai, Laura, Kumar, Deepak, and Dahiya, Rajvir
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Genetics ,Aging ,Prostate Cancer ,Urologic Diseases ,Biotechnology ,Cancer ,2.1 Biological and endogenous factors ,Aetiology ,Apoptosis ,Cell Line ,Tumor ,Cell Proliferation ,Down-Regulation ,Humans ,Incidence ,Male ,MicroRNAs ,Prostatic Neoplasms ,race related prostate cancer ,miRNA ,pathway modulation ,DNA methylation ,Oncology and Carcinogenesis - Abstract
The incidence of prostate cancer (PCa) among African-Americans (AfA) is significantly higher than Caucasian-Americans (CaA) but the genetic basis for this disparity is not known. To address this problem, we analyzed miRNA expression in AfA (n = 81) and CaA (n = 51) PCa patients. Here, we found that miR-24 is differentially expressed in AfA and CaA PCa patients and attempt to clarify its role in AfA patients. Also, the public sequencing data of the miR-24 promoter confirmed that it was highly methylated and down-regulated in PCa patients. Utilizing a VAMCSF and NDRI patient cohorts, we discovered that miR-24 expression was linked to a racial difference between AfA/CaA PCa patients. Interestingly, miR-24 was restored after treatment of PCa cells with 5Aza-CdR in an AfA cell line (MDA-PCa-2b), while restoration of miR-24 was not observed in CaA cells, DU-145. Ectopic expression of miR-24 showed decreased growth and induced apoptosis, though the effect was less in the CaA cell line compared to the AfA cell line. Finally, we found unique changes in biological pathways and processes associated with miR-24 transfected AfA cells by quantitative PCR-based gene expression array. Evaluation of the altered pathways showed that AR, IGF1, IGFBP5 and ETV1 were markedly decreased in the AfA derived cell line compared with CaA cells, and there was a reciprocal regulatory relationship of miR-24/target expression in prostate cancer patients. These results demonstrate that miR-24 may be a central regulator of key events that contribute to race-related tumorigenesis and has potential to be a therapeutic agent for PCa treatment.
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- 2017
8. Differential expression of miR-34b and androgen receptor pathway regulate prostate cancer aggressiveness between African-Americans and Caucasians
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Shiina, Marisa, Hashimoto, Yutaka, Kato, Taku, Yamamura, Soichiro, Tanaka, Yuichiro, Majid, Shahana, Saini, Sharanjot, Varahram, Shahryari, Kulkarni, Priyanka, Dasgupta, Pritha, Mitsui, Yozo, Sumida, Mitsuho, Tabatabai, Laura, Deng, Guoren, Kumar, Deepak, and Dahiya, Rajvir
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Biomedical and Clinical Sciences ,Clinical Sciences ,Oncology and Carcinogenesis ,Genetics ,Cancer ,Aging ,Urologic Diseases ,Prostate Cancer ,2.1 Biological and endogenous factors ,Aetiology ,African Americans ,Apoptosis ,Cell Line ,Tumor ,Cell Proliferation ,Chromosome Deletion ,DNA Methylation ,DNA-Binding Proteins ,G1 Phase Cell Cycle Checkpoints ,Gene Expression Regulation ,Neoplastic ,Humans ,Male ,MicroRNAs ,Promoter Regions ,Genetic ,Prostatic Neoplasms ,Receptors ,Androgen ,Signal Transduction ,Time Factors ,Transcription Factors ,Transfection ,Whites ,miR-34b ,prostate cancer ,african-americans ,caucasians ,androgen receptor ,White People ,Black or African American ,Oncology and carcinogenesis - Abstract
African-Americans are diagnosed with more aggressive prostate cancers and have worse survival than Caucasians, however a comprehensive understanding of this health disparity remains unclear. To clarify the mechanisms leading to this disparity, we analyzed the potential involvement of miR-34b expression in African-Americans and Caucasians. miR-34b functions as a tumor suppressor and has a multi-functional role, through regulation of cell proliferation, cell cycle and apoptosis. We found that miR-34b expression is lower in human prostate cancer tissues from African-Americans compared to Caucasians. DNA hypermethylation of the miR-34b-3p promoter region showed significantly higher methylation in prostate cancer compared to normal samples. We then sequenced the promoter region of miR-34b-3p and found a chromosomal deletion in miR-34b in African-American prostate cancer cell line (MDA-PCA-2b) and not in Caucasian cell line (DU-145). We found that AR and ETV1 genes are differentially expressed in MDA-PCa-2b and DU-145 cells after overexpression of miR-34b. Direct interaction of miR-34b with the 3' untranslated region of AR and ETV1 was validated by luciferase reporter assay. We found that miR-34b downregulation in African-Americans is inversely correlated with high AR levels that lead to increased cell proliferation. Overexpression of miR-34b in cell lines showed higher inhibition of cell proliferation, apoptosis and G1 arrest in the African-American cells (MDA-PCa-2b) compared to Caucasian cell line (DU-145). Taken together, our results show that differential expression of miR-34b and AR are associated with prostate cancer aggressiveness in African-Americans.
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- 2017
9. Oncogenic microRNA-4534 regulates PTEN pathway in prostate cancer
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Nip, Hannah, Dar, Altaf A, Saini, Sharanjot, Colden, Melissa, Varahram, Shahryari, Chowdhary, Harshika, Yamamura, Soichiro, Mitsui, Yozo, Tanaka, Yuichiro, Kato, Taku, Hashimoto, Yutaka, Shiina, Marisa, Kulkarni, Priyanka, Dasgupta, Pritha, Imai-sumida, Mitsuho, Tabatabai, Z Laura, Greene, Kirsten, Deng, Guoren, Dahiya, Rajvir, and Majid, Shahana
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Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Aging ,Biotechnology ,Prostate Cancer ,Urologic Diseases ,Cancer ,Genetics ,Detection ,screening and diagnosis ,2.1 Biological and endogenous factors ,4.1 Discovery and preclinical testing of markers and technologies ,Aetiology ,3' Untranslated Regions ,Aged ,Aged ,80 and over ,Animals ,Apoptosis ,Cell Line ,Cell Line ,Tumor ,Cell Movement ,DNA Methylation ,G1 Phase Cell Cycle Checkpoints ,Gene Expression Regulation ,Neoplastic ,Gene Knockdown Techniques ,Humans ,Male ,Mice ,Nude ,MicroRNAs ,Middle Aged ,PTEN Phosphohydrolase ,Prostatic Neoplasms ,Signal Transduction ,Transplantation ,Heterologous ,microRNA ,prostate cancer ,oncogene ,PTEN ,miR-4534 ,Oncology and carcinogenesis - Abstract
Prostate carcinogenesis involves alterations in several signaling pathways, the most prominent being the PI3K/AKT pathway. This pathway is constitutively active and drives prostate cancer (PCa) progression to advanced metastatic disease. PTEN, a critical tumor and metastasis suppressor gene negatively regulates cell survival, proliferation, migration and angiogenesis via the PI3K/Akt pathway. PTEN is mutated, downregulated/dysfunctional in many cancers and its dysregulation correlates with poor prognosis in PCa. Here, we demonstrate that microRNA-4534 (miR-4534) is overexpressed in PCa and show that miR-4534 is hypermethylated in normal tissues and cell lines compared to PCa tissues/cells. miR-4534 exerts its oncogenic effects partly by downregulating the tumor suppressor PTEN gene. Knockdown of miR-4534 impaired cell proliferation, migration/invasion and induced G0/G1 cell cycle arrest and apoptosis in PCa. Suppression of miR-4534 and its effects on tumor growth was confirmed in a xenograft mouse model. We performed parallel experiments in non-cancer RWPE1 cells by overexpessing miR-4534 followed by functional assays. Overexpression of miR-4534 induced pro-cancerous characteristics in this non-cancer cell line. Statistical analyses revealed that miR-4534 has potential to independently distinguish malignant from normal tissues and positively correlated with poor overall and PSA recurrence free survival. Taken together, our results show that depletion of miR-4534 in PCa induces a tumor suppressor phenotype partly through induction of PTEN. These results have important implications for identifying and defining the role of new PTEN regulators such as microRNAs in prostate tumorigenesis. Understanding aberrantly overexpressed miR-4534 and its downregulation of PTEN will provide mechanistic insight and therapeutic targets for PCa therapy.
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- 2016
10. Diagnostic potential of serum extracellular vesicles expressing prostate-specific membrane antigen in urologic malignancies
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Kawakami, Kyojiro, Fujita, Yasunori, Kato, Taku, Horie, Kengo, Koie, Takuya, Umezawa, Keitaro, Tsumoto, Hiroki, Miura, Yuri, Katagiri, Yasuo, Miyazaki, Tatsuhiko, Ohsawa, Ikuroh, Mizutani, Kosuke, and Ito, Masafumi
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- 2021
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11. CD44v8-10 mRNA contained in serum exosomes as a diagnostic marker for docetaxel resistance in prostate cancer patients
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Kato, Taku, Mizutani, Kosuke, Kawakami, Kyojiro, Fujita, Yasunori, Ehara, Hidetoshi, and Ito, Masafumi
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- 2020
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12. Functional role and tobacco smoking effects on methylation of CYP1A1 gene in prostate cancer
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Mitsui, Yozo, Chang, Inik, Kato, Taku, Hashimoto, Yutaka, Yamamura, Soichiro, Fukuhara, Shinichiro, Wong, Darryn K, Shiina, Marisa, Imai-Sumida, Mitsuho, Majid, Shahana, Saini, Sharanjot, Shiina, Hiroaki, Nakajima, Koichi, Deng, Guoren, Dahiya, Rajvir, and Tanaka, Yuichiro
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Aging ,Prevention ,Tobacco Smoke and Health ,Prostate Cancer ,Tobacco ,Biotechnology ,Cancer ,Urologic Diseases ,Genetics ,2.1 Biological and endogenous factors ,Aetiology ,Aged ,Aged ,80 and over ,Apoptosis ,Azacitidine ,Cell Line ,Tumor ,CpG Islands ,Cytochrome P-450 CYP1A1 ,DNA Methylation ,Decitabine ,Enhancer Elements ,Genetic ,Epigenesis ,Genetic ,Gene Expression Profiling ,Gene Expression Regulation ,Neoplastic ,Humans ,Male ,Middle Aged ,Oligonucleotide Array Sequence Analysis ,Polymerase Chain Reaction ,Promoter Regions ,Genetic ,Prostatic Hyperplasia ,Prostatic Neoplasms ,Sulfites ,Tissue Array Analysis ,Tobacco Smoking ,Xenobiotics ,cytochrome P450 1A1 ,methylation ,prostate cancer ,tobacco smoking ,Oncology and Carcinogenesis - Abstract
Cytochrome P450 (CYP) 1A1 is a phase I enzyme that can activate various compounds into reactive forms and thus, may contribute to carcinogenesis. In this study, we investigated the expression, methylation status, and functional role of CYP1A1 on prostate cancer cells. Increased expression of CYP1A1 was observed in all cancer lines (PC-3, LNCaP, and DU145) compared to BPH-1 (P < 0.05); and was enhanced further by 5-aza-2'-deoxycytidine treatment (P < 0.01). Methylation-specific PCR (MSP) and sequencing of bisulfite-modified DNA of the xenobiotic response element (XRE) enhancer site XRE-1383 indicated promoter methylation as a regulator of CYP1A1 expression. In tissue, microarrays showed higher immunostaining of CYP1A1 in prostate cancer than normal and benign prostatic hyperplasia (BPH; P < 0.001), and methylation analyses in clinical specimens revealed significantly lower methylation levels in cancer compared to BPH at all enhancer sites analyzed (XRE-1383, XRE-983, XRE-895; P < 0.01). Interestingly, smoking affected the XRE-1383 site where the methylation level was much lower in cancer tissues from smokers than non-smokers (P < 0.05). CYP1A1 levels are thus increased in prostate cancer and to determine the functional effect of CYP1A1 on cells, we depleted the gene in LNCaP and DU145 by siRNA. We observe that CYP1A1 knockdown decreased cell proliferation (P < 0.05) and increased apoptosis (P < 0.01) in both cell lines. We analyzed genes affected by CYP1A1 silencing and found that apoptosis-related BCL2 was significantly down-regulated. This study supports an oncogenic role for CYP1A1 in prostate cancer via promoter hypomethylation that is influenced by tobacco smoking, indicating CYP1A1 to be a promising target for prostate cancer treatment.
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- 2016
13. Photochromism of salicylideneanilines bearing super bulky substituents: Single-crystal UV-vis spectroscopic examination of bleaching under variable temperature and visible-light irradiation
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Suzuki, Yoshikazu, Kato, Taku, Huang, Hongyi, Yoshikawa, Isao, Mutai, Toshiki, and Houjou, Hirohiko
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- 2019
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14. Efficacy and safety of cytokines versus first-line sunitinib and second-line axitinib for patients with metastatic renal cell carcinoma (ESCAPE study): A study protocol for phase III randomized sequential open-label study
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Kadono, Yoshifumi, Konaka, Hiroyuki, Izumi, Kouji, Anai, Satoshi, Fujimoto, Kiyohide, Ishibashi, Kei, Kawai, Noriyasu, Kato, Taku, Iba, Akinori, Masumori, Naoya, Yoshimura, Kenichi, and Mizokami, Atsushiu
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- 2019
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15. Etiological role of human papillomavirus infection in the development of penile cancer
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Sakamoto, Jiro, Shigehara, Kazuyoshi, Nakashima, Kazufumi, Kawaguchi, Shohei, Nakashima, Takao, Shimamura, Masayoshi, Yasuda, Mitsuru, Kato, Taku, Hasegawa, Toru, Kobori, Yoshitomo, Okada, Hiroshi, Deguchi, Takashi, Izumi, Kouji, Kadono, Yoshifumi, and Mizokami, Atsushi
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- 2019
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16. Accumulation of intracellular S-adenosylmethionine increases the fermentation rate of bottom-fermenting brewer's yeast during high-gravity brewing
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Oomuro, Mayu, Watanabe, Daisuke, Sugimoto, Yukiko, Kato, Taku, Motoyama, Yasuo, Watanabe, Tetsuya, and Takagi, Hiroshi
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- 2018
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17. Defective quiescence entry promotes the fermentation performance of bottom-fermenting brewer's yeast
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Oomuro, Mayu, Kato, Taku, Zhou, Yan, Watanabe, Daisuke, Motoyama, Yasuo, Yamagishi, Hiromi, Akao, Takeshi, and Aizawa, Masayuki
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- 2016
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18. Development of flocculent Saccharomyces cerevisiae strain GYK-10 for the selective fermentation of glucose/fructose in sugar mills
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Kato, Taku, Ohara, Satoshi, Fukushima, Yasuhiro, Sugimoto, Akira, Masuda, Takayuki, Yasuhara, Takaomi, and Yamagishi, Hiromi
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- 2016
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19. New clinical strain of Neisseria gonorrhoeae with decreased susceptibility to ceftriaxone, Japan
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Deguchi, Takashi, Yasuda, Mitsuru, Hatazaki, Kyoko, Kameyama, Koji, Horie, Kengo, Kato, Taku, Mizutani, Kohsuke, Seike, Kensaku, Tsuchiya, Tomohiro, Yokoi, Shigeaki, Nakano, Masahiro, and Yoh, Mutsumasa
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Neisseria gonorrhoeae -- Distribution -- Research ,Company distribution practices ,Health - Abstract
To the Editor: In 2009, 2010, and 2013, Neisseria gonorrhoeae strains H041 (ceftriaxone MIC of 2 mg/L), F89 (ceftriaxone MIC of 1 mg/L), and A8806 (ceftriaxone MIC of 0.5 mg/L) [...]
- Published
- 2016
20. Successful Percutaneous Fogarty Thrombectomy for Subacute Lower Limb Ischemia Due to Resistant Thrombus in the Popliteal Artery.
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Yanagiuchi, Takashi, Kato, Taku, Hanabusa, Katsuyuki, Ushimaru, Shunpei, Yokoi, Hirokazu, and Ze, Kan
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POPLITEAL artery , *THROMBECTOMY , *THROMBOSIS , *ISCHEMIA , *TISSUE scaffolds - Abstract
Objective: Unusual setting of medical care Background: Subacute lower limb ischemia occurs more than 14 days and less than 3 months from symptom onset. Although endovascular procedures are the preferred treatment choice for a viable and not immediately threatened limb in patients with acute lower limb ischemia (<14 days), percutaneous catheter-directed thrombolysis, percutaneous mechanical thrombectomy, or percutaneous thromboaspiration are not recommended, and no treatment strategy has yet been established for nonacute lower limb ischemia (>14 days). A percutaneous Fogarty thrombectomy, an endovascular thrombus removal procedure with the use of a large-caliber sheath and a Fogarty balloon catheter, has recently been reported as a less invasive alternative to open surgery in patients with acute lower limb ischemia. In this report, we use this technique for a case of subacute lower limb ischemia caused by a resistant thrombus. Case Report: A 73-year-old man with a diagnosis of essential thrombocythemia presented with symptoms of right lower limb ischemia, which started about a month before. The diagnosis was subacute lower limb ischemia due to a resistant thrombus in the popliteal artery. First, we attempted percutaneous thromboaspiration and prolonged dilation with a large-caliber balloon catheter, but there were still severe residual stenoses with delayed blood flow. Although vascular scaffold implantation might have achieved complete revascularization, we avoided it because of a high probability of stent fracture in the popliteal artery. Thus, we performed a subsequent percutaneous Fogarty thrombectomy immediately after the conventional endovascular recanalization failed, achieving complete revascularization and next-day discharge without any complications. Conclusions: A percutaneous Fogarty thrombectomy could be a new treatment option for subacute lower limb ischemia due to a resistant thrombus, which can be performed immediately after failure of the conventional endovascular recanalization. [ABSTRACT FROM AUTHOR]
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- 2022
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21. Successful Retrieval of a Stuck Guidewire by Guiding Catheter Lock Technique in Renal Artery Stenting
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Ota, Yutaro, Kato, Taku, Yanagiuchi, Takashi, Ushimaru, Shunpei, and Yokoi, Hirokazu
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- 2020
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22. Suppressor effect of catechol-O-methyltransferase gene in prostate cancer.
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Hashimoto, Yutaka, Shiina, Marisa, Maekawa, Shigekatsu, Kato, Taku, Shahryari, Varahram, Kulkarni, Priyanka, Dasgupta, Pritha, Yamamura, Soichiro, Saini, Sharanjot, Tabatabai, Z. Laura, Dahiya, Rajvir, and Tanaka, Yuichiro
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PROSTATE cancer patients ,CATECHOL-O-methyltransferase ,CELL lines ,CANCER cells ,CELL proliferation ,BIOMARKERS ,MICRORNA ,POLYMERASE chain reaction - Abstract
Catechol-estrogens can cause genetic mutations and to counteract their oncogenicity, the catechol-O-methyltransferase (COMT) gene is capable of neutralizing these reactive compounds. In this study, we determined the functional effects and regulation of COMT in prostate cancer. Both the Cancer Genome Atlas (TCGA) and immunohistochemical analysis of clinical specimens demonstrated a reduction of COMT expression in prostate cancer. Also, western analyses of prostate cancer cell lines show COMT levels to be minimal in DuPro and DU145 and thus, these cells were used for further analyses. Re-expression of COMT led to suppressed migration ability (wound healing assay) and enhanced apoptosis (flow cytometric analyses), and when challenged with 4-hydroxyestradiol, a marked reduction of cell proliferation (MTT assay) was observed. Xenograft growth in athymic mice also resulted in inhibition due to COMT. As a mechanism, western analyses show cleaved CASP3 and BID were increased whereas XIAP and cIAP2 were reduced due to COMT. As COMT expression is low in prostate cancer, its regulation was determined. Databases identified several miRNAs capable of binding COMT and of these, miR-195 was observed to be increased in prostate cancer according to TCGA. Real-time PCR validated upregulation of miR-195 in clinical prostate cancer specimens as well as DuPro and DU145 and interestingly, luciferase reporter showed miR-195 capable of binding COMT and overexpressing miR-195 could reduce COMT in cells. These results demonstrate COMT to play a protective role by activating the apoptosis pathway and for miR-195 to regulate its expression. COMT may thus be a potential biomarker and gene of interest for therapeutic development for prostate cancer. [ABSTRACT FROM AUTHOR]
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- 2021
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23. Primary percutaneous coronary intervention without stenting using excimer laser and manual thrombectomy in STEMI with duodenal ulcer perforation: a case report.
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Yokoi, Hirokazu, Yanagiuchi, Takashi, Ushimaru, Shunpei, and Kato, Taku
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PERCUTANEOUS coronary intervention ,EXCIMER lasers ,THROMBECTOMY ,MYOCARDIAL infarction ,PEPTIC ulcer ,ELECTROCARDIOGRAPHY - Abstract
Background ST-segment elevation myocardial infarction (STEMI) and peptic ulcer perforation are both medical emergencies that require urgent intervention. In case that these time-sensitive medical emergencies present concomitantly, it remains unclear which one should be treated first. Case summary An 85-year-old man with melaena, epigastric pain, and severe anaemia was transferred to our emergency department and diagnosed as having inferior STEMI based on electrocardiogram. Emergency coronary angiography (CAG) revealed severe stenosis with thrombus in the proximal right coronary artery. Immediate oesophagogastroduodenoscopy and abdominal computed tomography detected the presence of duodenal ulcer perforation. Primary percutaneous coronary intervention (PCI) without stenting using excimer laser coronary angioplasty and manual thrombectomy was performed under intravascular ultrasound (IVUS) guidance to avoid dual antiplatelet therapy (DAPT). After successful PCI, the perforated viscus was surgically repaired with a laparoscopic omental patch. On Day 7, endoscopic haemostasis treated the oozing of blood from the duodenal ulcer. On Day 21, follow-up CAG and IVUS showed residual stenosis with organized thrombus in the culprit lesion, in which a drug-coated stent was directly implanted. He was discharged with a favourable clinical course on Day 23. Discussion We judged that PCI should take precedence over the surgical repair of perforated duodenal ulcer in our case since STEMI was an immediate life-threatening compared to the perforated viscus which had no active exsanguination. Excimer laser coronary angioplasty with manual thrombectomy might be an adequate option to avoid stent deployment and subsequent DAPT in such complex scenarios. [ABSTRACT FROM AUTHOR]
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- 2020
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24. A rare appearance of a large mural thrombus in left atrium detected two years after the Maze procedure
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Taminishi, Shunta, Nomura, Tetsuya, Kubota, Hiroshi, Miyawaki, Daisuke, Urata, Ryota, Sugimoto, Takeshi, Higuchi, Yusuke, Kato, Taku, Keira, Natsuya, Doi, Kiyoshi, Yaku, Hitoshi, and Tatsumi, Tetsuya
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- 2016
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25. Dietary protein restriction increases hepatic leptin receptor mRNA and plasma soluble leptin receptor in male rodents.
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Yamada, Riho, Odamaki, Shizuka, Araki, Masaya, Watanabe, Tasuku, Matsuo, Keigo, Uchida, Kaito, Kato, Taku, Ozaki-Masuzawa, Yori, and Takenaka, Asako
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LEPTIN receptors ,LEPTIN ,MESSENGER RNA ,PROTEINS ,LOW-protein diet ,RODENTS - Abstract
Leptin is an adipokine that regulates adipose tissue mass through membrane-anchored leptin receptor (Ob-R). Extracellular domain of Ob-R in plasma is called soluble leptin receptor (sOb-R), and is the main leptin-binding protein. Based on a previous DNA microarray analysis that showed induction of hepatic Ob-R mRNA in low-protein diet-fed mice, this study aimed to clarify the effect of dietary protein restriction on hepatic Ob-R mRNA and plasma sOb-R levels. First, the effect of protein restriction on hepatic Ob-R mRNA level was examined together with fasting and food restriction using male rats as common experimental model for nutritional research. Hepatic Ob-R mRNA level was increased by feeding low-protein diet for 7 d, although not significantly influenced by 12-h fasting and sixty percent restriction in food consumption. Then, effect of protein restriction on liver Ob-R and plasma sOb-R was investigated using male mice because specific sOb-R ELISA was more available for mice. Hepatic Ob-R mRNA level was also increased in protein restricted-mice although it did not increase in hypothalamus. Hepatic Ob-R protein was decreased, whereas plasma sOb-R was increased by protein restriction. Because the concentration of sOb-R increased without changing plasma leptin concentration, free leptin in plasma was significantly reduced. The direct effect of amino acid deprivation on Ob-R mRNA level was not observed in rat hepatoma cells H4IIE cultured in amino acid deprived medium. In conclusion, dietary protein restriction increased hepatic Ob-R mRNA, resulting in increased plasma sOb-R concentration, which in turn, reduces plasma free leptin level and may modulate leptin activity. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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26. Spontaneous healing of rectal penetration by SpaceOAR® hydrogel insertion during permanent iodine-125 implant brachytherapy: A case report.
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Iinuma, Koji, Mizutani, Kosuke, Kato, Taku, Nakane, Keita, Tanaka, Hidekazu, Nakano, Masahiro, Matsuo, Masayuki, and Koie, Takuya
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HEALING ,POLYETHYLENE glycol ,RADIOISOTOPE brachytherapy ,RADIOTHERAPY ,RADIATION doses ,RECTAL prolapse - Abstract
Radiation therapy with permanent iodine-125 implant brachytherapy is well established for curable prostate cancer. To maximize the therapeutic potential of brachytherapy, delivered radiation doses have been increased accompanied by refined intraoperative procedures of radioactive seeds placement. However, dose escalation is a double-edged sword in prostate brachytherapy; it could induce rectal toxicity, yet is successful in curable treatment in prostate cancer. To reduce irradiation of the rectum, the SpaceOAR
® system has been used to inject synthetic polyethylene glycol hydrogel between the prostate and the rectum. The present report describes a case of spontaneous healing of rectal penetration associated with SpaceOAR® hydrogel. [ABSTRACT FROM AUTHOR]- Published
- 2019
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27. TCTAP A-125 A Newly Designed Guiding Sheath Provides a Novel Option for a Crossover Approach in Endovascular Therapy
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Wakana, Noriyuki, Zen, Kan, Yakabe, Kazuaki, Kambayashi, Daisuke, Kato, Taku, Tsubakimoto, Yoshinori, Nakagami, Takuo, Yamaguchi, Shinichiro, Yanishi, Kenji, Nakanishi, Naohiko, Nakamura, Takeshi, and Matoba, Satoaki
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- 2017
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28. Gamma-glutamyltransferase activity in exosomes as a potential marker for prostate cancer.
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Kyojiro Kawakami, Yasunori Fujita, Yoko Matsuda, Tomio Arai, Kengo Horie, Koji Kameyama, Taku Kato, Koichi Masunaga, Yutaka Kasuya, Masashi Tanaka, Kosuke Mizutani, Takashi Deguchi, Masafumi Ito, Kawakami, Kyojiro, Fujita, Yasunori, Matsuda, Yoko, Arai, Tomio, Horie, Kengo, Kameyama, Koji, and Kato, Taku
- Subjects
DIAGNOSIS ,PROSTATE cancer ,PHYSIOLOGICAL effects of glutamine ,EXOSOMES ,PROSTATE-specific membrane antigen ,WESTERN immunoblotting ,ANTIGENS ,CELL lines ,GENES ,PROSTATE tumors ,PROTEOLYTIC enzymes ,BENIGN prostatic hyperplasia ,GAMMA-glutamyltransferase - Abstract
Background: Exosomes or extracellular vesicles have the potential as a diagnostic marker for various diseases including cancer. In order to identify novel exosomal markers for prostate cancer (PC), we performed proteomic analysis of exosomes isolated from PC cell lines and examined the usefulness of the marker in patients.Methods: Exosomes isolated by differential centrifugation from the culture medium of androgen-dependent LNCaP prostate cancer cell line and its sublines of partially androgen-independent C4, androgen-independent C4-2 and bone metastatic C4-2B were subjected to iTRAQ-based proteomic analysis. Exosomes were also isolated by immunocapture and separated by size exclusion chromatography and density gradient centrifugation. Protein expression was determined by Western blot analysis. GGT activity was measured using a fluorescent probe, γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG). Immunohistochemical analysis of tissues was performed using anti-GGT1 antibody.Results: Among proteins upregulated in C4-2 and C4-2B cells than in LNCaP cells, we focused on gamma-glutamyltransferase 1 (GGT1), a cell-surface enzyme that regulates the catabolism of extracellular glutathione. The levels of both GGT1 large and small subunits were elevated in exosomes isolated from C4-2 and C4-2B cells by differential centrifugation and by immunocapture with anti-CD9 or -prostate-specific membrane antigen (PSMA) antibody. In cell lysates and exosomes, GGT1 expression correlated with GGT activity. Size exclusion chromatography of human serum demonstrated the presence of GGT activity and GGT1 subunits in fractions positive for CD9. Density gradient centrifugation revealed the co-presence of GGT1 subunits with CD9 in exosomes isolated by differential centrifugation from human serum. Since GGT activity correlated with GGT1 expression in serum exosomes isolated by differential centrifugation, we measured serum exosomal GGT activity in patients. Unexpectedly, we found that serum exosomal GGT activity was significantly higher in PC patients than in benign prostatic hyperplasia (BPH) patients. In support of this finding, immunohistochemical analysis showed increased GGT1 expression in PC tissues compared with BPH tissues.Conclusions: Our results suggest that serum exosomal GGT activity could be a useful biomarker for PC. [ABSTRACT FROM AUTHOR]- Published
- 2017
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29. Instructive percutaneous coronary intervention to avoid the risk of side branch occlusion at a lesion with a lotus root appearance: a case report.
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Tetsuya Nomura, Taku Kato, Hiroshi Kubota, Daisuke Miyawaki, Ryota Urata, Takeshi Sugimoto, Yusuke Higuchi, Natsuya Keira, Tetsuya Tatsumi, Nomura, Tetsuya, Kato, Taku, Kubota, Hiroshi, Miyawaki, Daisuke, Urata, Ryota, Sugimoto, Takeshi, Higuchi, Yusuke, Keira, Natsuya, and Tatsumi, Tetsuya
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PERCUTANEOUS coronary intervention ,ARTERIAL occlusions ,TISSUE wounds ,RIGHT heart ventricle ,OPTICAL coherence tomography ,BIFURCATION theory ,DISEASE risk factors - Abstract
Background: A lotus root appearance is a rare entity, and there is little opportunity to perform coronary intervention for this kind of lesion. Because of its peculiar anatomical characteristics, one of the problems regarding percutaneous coronary intervention (PCI) for these lesions is related to the involvement of branch vessels.Case Presentation: We encountered a case of PCI for a stenotic lesion with a lotus root appearance in the mid-portion of the right coronary artery (RCA). To avoid the risk of right ventricular (RV) branch occlusion due to stent deployment in the main RCA, we re-crossed the third guidewire into the main RCA via the nearest point to the RV branch ostium through the communicating vascular lumen. Thereafter, we deployed a drug-eluting stent in the main RCA crossing over the RV branch, and the ostium of the RV branch remained intact, as we expected.Conclusions: This case is the first report in the world describing the details of how to maintain the patency of the side branch bifurcating from a lesion with a lotus root appearance under optical coherence tomography guidance. [ABSTRACT FROM AUTHOR]- Published
- 2016
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30. Dipeptidyl peptidase-4 inhibitor sitagliptin improves pancreatic β-cell function in hypertensive diabetic patients treated with angiotensin receptor blockers.
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Fukui, Kensuke, Kawahito, Hiroyuki, Wakana, Noriyuki, Kikai, Masakazu, Terada, Kensuke, Yamamoto, Keita, Irie, Daisuke, Kato, Taku, Miyagawa, Sonoko, and Yamada, Hiroyuki
- Abstract
Introduction: Dipeptidyl peptidase (DPP)-4 inhibitors, a novel oral anti-diabetic agents, exert a protective effect on pancreatic β-cell function in patients with type 2 diabetic mellitus (T2DM). However, their beneficial effect in hypertensive T2DM patients treated with angiotensin receptor blockers (ARBs) has not been investigated.Methods: In this open-label multicenter randomized study, a total of 55 hypertensive T2DM patients treated with ARBs were randomly assigned to receive the DPP-4 inhibitor sitagliptin or sulfonylurea (SU).Results: After 24 weeks of treatment, a significant reduction in fasting blood glucose was only observed in the sitagliptin group, while HbA1c was significantly reduced in both groups. Homeostasis model assessment of insulin resistance was not significantly improved in either group. Indicators of pancreatic β-cell function, including proinsulin to insulin ratio and homeostasis model assessment of β-cell function, were significantly improved in the sitagliptin group, but not in the SU group. The beneficial effects of sitagliptin were observed in hypoglycemic drug naïve patients, but not in patients who had received SU monotherapy prior to the study.Conclusion: Treatment with the DPP-4 inhibitor sitagliptin might exert beneficial effects on pancreatic β-cell function in ARB-treated T2DM patients and its efficacy might be more pronounced in hypoglycemic drug naïve patients. [ABSTRACT FROM AUTHOR]- Published
- 2015
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31. Bone marrow angiotensin AT2 receptor deficiency aggravates atherosclerosis development by eliminating macrophage liver X receptor-mediated anti-atherogenic actions.
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Kato, Taku, Kawahito, Hiroyuki, Kishida, Sou, Irie, Daisuke, Wakana, Noriyuki, Kikai, Masakazu, Takata, Hiroki, Ogata, Takehiro, Ueyama, Tomomi, Matoba, Satoaki, and Yamada, Hiroyuki
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CHOLESTEROL metabolism ,ANIMAL experimentation ,APOLIPOPROTEINS ,ATHEROSCLEROSIS ,BIOLOGICAL transport ,BONE marrow ,CELL physiology ,CELL receptors ,GENES ,INFLAMMATION ,MACROPHAGES ,MICE ,PHENOTYPES - Abstract
Background: Bone marrow (BM) Angiotensin II (Ang II) type 1 (AT1) receptor plays a crucial role in atherosclerosis development; however, the effect of BM Ang II type 2 (AT2) receptor on atherogenesis remains undefined.Methods and Results: We generated BM chimera apoE-deficient (apoE(-/-)) mice whose BM cells were repopulated with AT2-deficient (Agtr2(-/-)) or wild-type (Agtr2(+/+)) cells. After 2 months of a high-cholesterol diet, the atherosclerotic lesion area was significantly increased in the apoE(-/-)/BM-Agtr2(-/-) mice compared with the apoE(-/-)/BM-Agtr2(+/+) mice (51%, P < 0.05), accompanied by an augmented accumulation of lesion macrophages. Although phenotypic polarization in BM-derived macrophages and lipopolysaccharide-induced expression of proinflammatory cytokines in thioglycollate-induced peritoneal macrophages (TGPMs) were not affected by AT2-deficiency, mRNA and protein expression levels of macrophage liver X receptor β (LXRβ) were significantly decreased in Agtr2(-/-) TGPMs compared with Agtr2(+/+) TGPMs. Anti-inflammatory effects of LXR agonist (GW3965) were markedly inhibited in Agtr2(-/-) TGPMs. Furthermore, the expression levels of ATP-binding cassette transporter ABCA1 and CCR7 were much lower in Agtr2(-/-) TGPMs than Agtr2(+/+) TGPMs, accompanied by a significantly reduced cholesterol efflux.Conclusions: Our findings demonstrate that BM-AT2 deficiency aggravates atherosclerosis, at least in part, by eliminating the anti-atherogenic properties of macrophages elicited by LXRβ activation. [ABSTRACT FROM AUTHOR]- Published
- 2015
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32. Vascular angiotensin II type 2 receptor attenuates atherosclerosis via a kinin/NO-dependent mechanism.
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Takata, Hiroki, Yamada, Hiroyuki, Kawahito, Hiroyuki, Kishida, Sou, Irie, Daisuke, Kato, Taku, Wakana, Noriyuki, Miyagawa, Sonoko, Fukui, Kensuke, and Matsubara, Hiroaki
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- 2015
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33. Transplantation of periaortic adipose tissue from angiotensin receptor blocker-treated mice markedly ameliorates atherosclerosis development in apoE–/– mice.
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Irie, Daisuke, Kawahito, Hiroyuki, Wakana, Noriyuki, Kato, Taku, Kishida, Sou, Kikai, Masakazu, Ogata, Takehiro, Ikeda, Koji, Ueyama, Tomomi, Matoba, Satoaki, and Yamada, Hiroyuki
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- 2015
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34. New Clinical Strain of Neisseria gonorrhoeae with Decreased Susceptibility to Ceftriaxone, Japan.
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Takashi Deguchi, Mitsuru Yasuda, Kyoko Hatazaki, Koji Kameyama, Kengo Horie, Taku Kato, Kohsuke Mizutani, Kensaku Seike, Tomohiro Tsuchiya, Shigeaki Yokoi, Masahiro Nakano, Mutsumasa Yoh, Deguchi, Takashi, Yasuda, Mitsuru, Hatazaki, Kyoko, Kameyama, Koji, Horie, Kengo, Kato, Taku, Mizutani, Kohsuke, and Seike, Kensaku
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NEISSERIA gonorrhoeae ,MICROBIAL sensitivity tests ,CEFTRIAXONE ,GONORRHEA - Abstract
This article presents a study on a clinical Neisseria gonorrhoeae strain, known as GU140106, with decreased susceptibility to ceftriaxone in Japan. It discusses the isolation of the strain from a male patient with acute urethritis, treatment given to the patient and tests that confirmed the strain as a gonococcal species.
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- 2016
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35. Urinary exosome as a potential biomarker for urinary tract infection.
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Mizutani, Kosuke, Kawakami, Kyojiro, Horie, Kengo, Fujita, Yasunori, Kameyama, Koji, Kato, Taku, Nakane, Keita, Tsuchiya, Tomohiro, Yasuda, Mitsuru, Masunaga, Koichi, Kasuya, Yutaka, Masuda, Yoshishige, Deguchi, Takashi, Koie, Takuya, and Ito, Masafumi
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URINARY tract infections ,WESTERN immunoblotting ,EXOSOMES ,WOMEN patients ,PROTEIN expression ,CELL culture - Abstract
Unlike urinary tract infection (UTI), asymptomatic bacteriuria (ABU) should not be treated, with some exceptions such as pregnant women and patients who will undergo traumatic urologic interventions. However, there has been no clinically available marker for their differential diagnosis. Exosomes or small extracellular vesicles carry proteins contained in cells from which they are derived, thus having the potential as a biomarker of several diseases. On the basis of the hypothesis that the molecular signature of exosomes in urine may differ between UTI and ABU patients, we examined if urinary exosomes could serve as a marker for their differential diagnosis. Exosomes were isolated by ultracentrifugation or affinity‐based method from cell culture medium of monocytic THP‐1 and uroepithelial SV‐HUC‐1 cells and human urine. Protein expression was examined by Western blot analysis, ELISA, and CLEIA. The results showed that the levels of intracellular signalling molecules Akt and ERK and transcription factor NF‐κB increased in exosomes isolated from THP‐1 and SV‐HUC‐1 cells cocultured with Escherichia coli and/or treated with lipopolysaccharide. In urinary exosomes of UTI patients, Akt significantly diminished, and an exosomal marker CD9 showed a trend to decrease after treatment with antimicrobial agents. More importantly, Akt and CD9 levels in urinary exosomes were higher in UTI patients than in ABU patients, which was also observed after correction by urine creatinine. Collectively, these results suggest that Akt and CD9 in urinary exosomes could be useful markers for differential diagnosis of UTI and ABU. [ABSTRACT FROM AUTHOR]
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- 2019
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36. CLINICAL OUTCOMES OF ENDOVASCULAR TREATMENT FOR CHRONIC AORTIC CCCLUSION.
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Kato, Taku, Zen, Kan, Kawarada, Osami, Hozawa, Koji, Anzai, Hitoshi, Nakamura, Hiroaki, Nomura, Tetsuya, Funatsu, Atsushi, Kawasaki, Daizo, Tsubakimoto, Yoshinori, Higashimori, Akihiro, and Matoba, Satoaki
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- *
ENDOVASCULAR surgery , *CLINICAL trials , *HEALTH outcome assessment , *AORTIC aneurysms , *TREATMENT effectiveness - Published
- 2016
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37. Successful percutaneous coronary intervention with GuideLiner® catheter for subtotal occlusive lesion in the right coronary artery with anomalous origin from the left sinus of Valsalva: a case report.
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Shirota, Ayumi, Nomura, Tetsuya, Kubota, Hiroshi, Taminishi, Shunta, Urata, Ryota, Sugimoto, Takeshi, Higuchi, Yusuke, Kato, Taku, Keira, Natsuya, and Tatsumi, Tetsuya
- Abstract
Introduction: Because of the unusual anatomy of an anomalous origin of the right coronary artery from the left sinus of Valsalva, selective cannulation of the guiding catheter in percutaneous coronary intervention for these cases is always challenging.Case Presentation: A 58-year-old Japanese man was admitted to our hospital complaining of worsening exertional chest pain. He was suspected of having unstable angina pectoris and underwent cardiac catheterization. We found a subtotal occlusive lesion in the mid-portion of his right coronary artery that originated from the left sinus of Valsalva. On the previous percutaneous coronary intervention, we failed to cannulate the guiding catheter to the anomalous orifice of the right coronary artery. Therefore, we decided to use the GuideLiner catheter for stable back-up support from the beginning. A 6Fr GuideLiner catheter was introduced into the right coronary artery by anchoring it coaxially with a semi-compliant balloon catheter. And we successfully deployed two drug-eluting stents by crossing over the posterior-descending artery. Final angiography demonstrated favorable dilatation of the target lesion, and native blood flow in the right coronary artery was completely recovered.Conclusion: GuideLiner is a monorail-type "child" support catheter that facilitates coaxial guiding catheter engagement and an appropriate back-up force, achieving successful device delivery to target lesions in this kind of complex percutaneous coronary intervention. [ABSTRACT FROM AUTHOR]- Published
- 2015
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38. Evaluation of salinity effect on quantitative analysis of aquatic humic substances using nonionic DAX-8 resin.
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Kida, Morimaru, Ohtsuka, Toshiyuki, Kato, Taku, Suzuki, Takeshi, and Fujitake, Nobuhide
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- *
HUMUS , *GUMS & resins , *HYDROPHOBIC interactions , *SEAWATER , *DISSOLVED organic matter , *SOLUTION (Chemistry) - Abstract
A nonionic macroporous resin, Amberlite ® XAD-8, or its substitute, Supelite™ DAX-8, is used when isolating or quantifying aquatic humic substances (AHS). However, the effect of salinity on the adsorption behavior of AHS onto the resin is yet to be confirmed, rendering the possibility of salinity-induced changes in the values of quantified amounts or characteristics of AHS obtained from a salty system. To verify the results of quantification and isolation of AHS using the resin in different salinity systems, the effect of salinity on such quantitative analyses of AHS has been examined. It has been concluded that the salinity effect is in general trivial and will not hinder comparison of results regardless of sample solution salinity. [ABSTRACT FROM AUTHOR]
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- 2016
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39. Comparison of the Pre-Established and Finally Selected Treatment Strategies for Endovascular Treatment in Femoropopliteal Artery Lesions.
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Higashitani M, Ueshima D, Suzuki K, Yamauchi Y, Hirokami M, Tsubakimoto Y, Takahashi A, Kato T, Ando H, and Nakamura M
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- Humans, Femoral Artery surgery, Popliteal Artery surgery, Popliteal Artery pathology, Stents, Treatment Outcome, Vascular Patency, Multicenter Studies as Topic, Angioplasty, Balloon, Peripheral Arterial Disease surgery, Peripheral Arterial Disease etiology
- Abstract
This study aimed to compare lower limb events associated with preplanned and finally selected treatment strategies-the validity and usefulness of the physician-chosen strategy were verified.We examined the data of 1003 patients in the registry of multicenter endovascular treatment for superficial femoral and popliteal artery disease study and prospectively enrolled patients who underwent endovascular treatment (EVT) of the femoropopliteal (FP) artery between February 2017 and June 2018 from 67 Japanese institutes. The outcome measures were major adverse limb events (MALE) and target vessel revascularization.The EVT strategies were classified into balloon angioplasty-alone (37.3%), primary stenting (26.7%), and provisional stenting (36.0%) groups. In the initial strategy analysis for the balloon angioplasty-alone, primary stenting, and provisional stenting groups, two-year rates of freedom from MALE (95% confidence interval) were 0.680 (0.620-0.732), 0.754 (0.688-0.808), and 0.798 (0.746-0.840), respectively. Additionally, the rate of MALE was significantly higher among patients in the balloon angioplasty-alone group than among those in the primary or provisional stenting groups in the initial strategy analysis (P = 0.007). Changes in treatment strategy were more frequent in the primary stenting group than in the other groups. Furthermore, the rate of MALE did not significantly differ among the three groups in the final strategy analysis (P = 0.56).Limb outcomes for the final applied strategy did not differ among the three strategies. Additionally, the physician's selection bias was mostly appropriate in the EVT of the FP artery.
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- 2024
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40. Chromogenic in situ hybridization reveals specific expression pattern of long non-coding RNA DRAIC in formalin-fixed paraffin-embedded specimen.
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Sakurai K, Yamada S, Ito R, Ochiai M, Ando T, Sakai Y, Kato T, and Ito H
- Abstract
Long non-coding RNA (lncRNA) plays an important role in the regulation of gene expression in normal and cancer cells. We previously discovered a novel tumor-suppressive lncRNA, DRAIC , in prostate cancer cells. Subsequent studies have demonstrated that DRAIC is dysregulated in various malignancies and exhibits a tumor-suppressive or pro-oncogenic function. However, details regarding its expression pattern in normal and cancerous tissues remain largely unknown. In this study, we performed chromogenic in situ hybridization (CISH) using RNAscope technology to assess DRAIC expression in formalin-fixed paraffin-embedded (FFPE) specimens. In the neuroendocrine-differentiated cancer cell line VMRC-LCD, CISH revealed a diffuse localization of DRAIC in the cytoplasm as well as specific accumulation in the nuclear compartment. DRAIC expression was comprehensively analyzed using tissue microarrays containing 89 normal and 155 tumor tissue samples. DRAIC was weakly expressed in normal epithelial cells of the colon, bronchiole, kidney, prostate, and testis. Conversely, DRAIC was moderately to highly expressed in some cancer tissues, including prostate adenocarcinoma, invasive ductal carcinoma of the breast, neuroendocrine carcinoma of the esophagus, lung adenocarcinoma, and small cell lung carcinoma. While DRAIC knockdown did not affect VMRC-LCD cellular viability and invasive ability, gene expression related to the neuroendocrine and cancer-related pathways was altered. Our expression analysis revealed the specific expression pattern of DRAIC in normal and cancerous FFPE tissues. The results presented here may lead to the elucidation of additional novel functions of DRAIC ., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)
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- 2023
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41. H19 in Serum Extracellular Vesicles Reflects Resistance to AR Axis-targeted Therapy Among CRPC Patients.
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Kato T, Kawakami K, Mizutani K, Ando T, Sakai Y, Sakurai K, Toyota S, Ehara H, Ito H, and Ito M
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- Male, Humans, Androgen Antagonists, Proteomics, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Prostatic Neoplasms, Castration-Resistant drug therapy, Prostatic Neoplasms, Castration-Resistant genetics, Prostatic Neoplasms, Castration-Resistant metabolism, RNA, Long Noncoding genetics, Extracellular Vesicles metabolism
- Abstract
Background/aim: We aimed to evaluate the changes of androgen receptor (AR) signaling-related long non-coding RNAs (lncRNAs) in serum extracellular vesicles (EVs) from prostate cancer (PC) patients, in order to identify novel biomarkers for AR axis-targeted therapy (ARAT)-resistance among castration-resistant PC (CRPC) patients., Patients and Methods: EVs were isolated from 2 patients before and after acquiring ARAT-resistance. RNA profiling of EVs was performed by RNA-sequencing. The expression levels of selected lncRNAs in EVs were analyzed by digital droplet PCR (ddPCR) in 58 localized and 14 metastatic PC patients at diagnosis, 7 ARAT-naïve and 6 ARAT-resistant CRPC patients. LncRNA H19 expression in PC tissue was examined using published data. In order to analyze the role of H19, the prognosis was analyzed in PC patients and proteomic analysis was performed in 22Rv1 PC cells., Results: RNA-sequencing revealed that AR-regulated RNAs were most enriched in EVs after acquiring ARAT-resistance. Among them, up-regulation of AR signaling-related lncRNAs (PCAT1, H19, HOXA-11AS, ZEB1-AS1, ARLNC1, PART1, CTBP1-AS and PCA3) was confirmed by ddPCR. H19 contained in EVs (EV-H19) was significantly increased among ARAT-resistant patients compared to ARAT-naïve CRPC or metastatic PC patients. In PC tissue, H19 was negatively correlated with AR protein and AR-activity score and up-regulated in neuroendocrine CRPC tissue with low AR expression. Furthermore, EV-H19 expression was significantly associated with worse outcome to androgen-deprivation therapy. Proteomic analysis demonstrated that H19 knockdown enhanced PC-related protein expression., Conclusion: EV-H19 may negatively correlate with AR-signaling activity and could be a marker to diagnose ARAT-resistance among CRPC patients., (Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2023
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42. Ovariectomy Increases Circulating Retinol-Binding Protein Concentrations Independently of Sex-Dependent Differences in Retinol Concentrations in Rats.
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Moriya H, Yokobori Y, Furukawa T, Kato T, Sato R, and Takenaka A
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- Female, Male, Rats, Animals, Vitamin A, Rats, Wistar, Sex Characteristics, Retinol-Binding Proteins, Plasma genetics, Retinol-Binding Proteins, Plasma metabolism, Adipose Tissue metabolism, Insulin Resistance
- Abstract
Background: There is a sex-dependent difference in blood retinol and RBP concentrations, and plasma RBP is associated with insulin resistance., Objectives: We aimed to clarify sex-dependent variations in body concentrations of retinol and RBPs and their association with sex hormones in rats., Methods: Plasma and liver retinol concentrations and hepatic mRNA and plasma concentrations of RBP4 were analyzed in 3- and 8-wk-old male and female Wistar rats before and after sexual maturity (experiment 1) and in orchiectomized male Wistar rats (experiment 2) and ovariectomized female Wistar rats (experiment 3). Furthermore, the mRNA and protein concentrations of RBP4 in adipose tissue were measured in ovariectomized female rats (experiment 3)., Results: There were no sex-dependent differences in liver retinyl palmitate and retinol concentrations; however, the plasma retinol concentration was significantly higher in male rats than that in female rats after sexual maturity. Furthermore, the plasma retinol concentrations did not differ between the ovariectomized or orchiectomized rats and the control rats. Plasma Rbp4 mRNA concentrations were higher in male rats than those in female rats but not in castrated and control rats, a change consistent with plasma retinol concentration. Plasma RBP4 concentrations were also higher in male rats than those in female rats; however, unlike liver Rbp4 gene expression, plasma RBP4 concentrations were 7-fold higher in the ovariectomized rats than those in the control rats. Moreover, the Rbp4 mRNA concentrations in inguinal white adipose tissue was significantly higher in the ovariectomized rats than those in the control rats and correlated with plasma RBP4 concentrations., Conclusions: Hepatic Rbp4 mRNA is higher in male rats through a sex hormone-independent mechanism, which may contribute to sex differences in blood retinol concentrations. Furthermore, ovariectomy leads to an increase in adipose tissue Rbp4 mRNA and blood RBP4 concentrations, which may contribute to insulin resistance in ovariectomized rats and postmenopausal women., (Copyright © 2023 American Society for Nutrition. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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43. Cytomorphology and Gene Expression Signatures of Anchorage-independent Aggregations of Oral Cancer Cells.
- Author
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Sakurai K, Nagai A, Ando T, Sakai Y, Ideta Y, Hayashi Y, Baba J, Mitsudo K, Akita M, Yamamichi N, Fujigaki H, Kato T, and Ito H
- Subjects
- Humans, Cell Adhesion genetics, Transcriptome, Mouth Neoplasms genetics
- Abstract
Background/aim: Cancer cells with high anchorage independence can survive and proliferate in the absence of adhesion to the extracellular matrix. Under anchorage-independent conditions, cancer cells adhere to each other and form aggregates to overcome various stresses. In this study, we investigated the cytomorphology and gene expression signatures of oral cancer cell aggregates., Materials and Methods: Two oral cancer-derived cell lines, SAS and HSC-3 cells, were cultured in a low-attachment plate and their cytomorphologies were observed. The transcriptome between attached and detached SAS cells was examined using gene expression microarrays. Subsequently, gene enrichment analysis and Ingenuity Pathway Analysis were performed. Gene expression changes under attached, detached, and re-attached conditions were measured via RT-qPCR., Results: While SAS cells formed multiple round-shaped aggregates, HSC-3 cells, which had lower anchorage independence, did not form aggregates efficiently. Each SAS cell in the aggregate was linked by desmosomes and tight junctions. Comparative transcriptomic analysis revealed 1,698 differentially expressed genes (DEGs) between attached and detached SAS cells. The DEGs were associated with various functions and processes, including cell adhesion. Moreover, under the detached condition, the expression of some epithelial genes (DSC3, DSP, CLDN1 and OCLN) were up-regulated. The changes in both cytomorphology and epithelial gene expression under the detached condition overall returned to their original ones when cells re-attached., Conclusion: The results suggest specific cytomorphological and gene expression changes in oral cancer cell aggregates. Our findings provide insights into the mechanisms underlying anchorage-independent oral cancer cell aggregation and reveal previously unknown potential diagnostic and therapeutic molecules., (Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2023
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44. Double-Balloon Technique Using Optimo Balloon Guiding Catheter for Blood Flow Occlusion During Open Surgical Repair of Femoral Artery Pseudoaneurysm.
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Yanagiuchi T, Kato T, Kitani K, Yamazaki S, Ushimaru S, and Yokoi H
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- Catheters, Femoral Artery diagnostic imaging, Femoral Artery surgery, Humans, Aneurysm, False diagnosis, Aneurysm, False etiology, Aneurysm, False surgery, Angioplasty, Balloon methods, Balloon Occlusion, Vascular Diseases
- Published
- 2022
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45. miRNA-93 in Serum Extracellular Vesicles Before and After Low Dose Rate Prostate Brachytherapy.
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Iinuma K, Kawakami K, Mizutani K, Fujita Y, Yamaguchi T, Ito M, Kumano T, Matsuo M, Nakano M, Koie T, Ito M, and Kato T
- Subjects
- Down-Regulation radiation effects, Humans, Male, MicroRNAs blood, Phosphatidylinositol 3-Kinases metabolism, Prostatic Neoplasms blood, Proto-Oncogene Proteins c-akt metabolism, Radiotherapy Dosage, Signal Transduction genetics, Signal Transduction radiation effects, Time Factors, Treatment Outcome, Brachytherapy methods, Extracellular Vesicles genetics, Gene Expression Profiling methods, Gene Expression Regulation, Neoplastic radiation effects, MicroRNAs genetics, Prostatic Neoplasms radiotherapy
- Abstract
Background/aim: To identify novel biomarkers for prostate cancer (PC), we evaluated changes of miRNAs contained in serum small extracellular vesicles (EVs) in patients who received low dose rate prostate brachytherapy (BT)., Materials and Methods: EVs were isolated from the pooled serum of 10 PC patients prior to and 1 month after BT. miRNA profiling and quantitation in EVs was performed by microarray analysis and RT-digital PCR, respectively. Expression of miRNA-93 in prostate tissue was evaluated using the TCGA database and its level in EVs was determined in 25 patients before and 1, 3, 6 and 12 months after BT., Results: Profiling and quantitation identified miRNA-93 as significantly down-regulated in EVs after BT. TCGA database analysis showed that miRNA-93 was increased in PC tissue. miRNA-93 in EVs significantly decreased in 3, 6 and 12 months after BT., Conclusion: miRNA-93 contained in serum EVs may be a novel diagnostic and monitoring biomarker for PC., (Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2021
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46. Profiling of Serum Extracellular Vesicles Reveals miRNA-4525 as a Potential Biomarker for Advanced Renal Cell Carcinoma.
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Muramatsu-Maekawa Y, Kawakami K, Fujita Y, Takai M, Kato D, Nakane K, Kato T, Tsuchiya T, Koie T, Miura Y, Ito M, and Mizutani K
- Subjects
- Adult, Biomarkers, Tumor blood, Carcinoma, Renal Cell genetics, Carcinoma, Renal Cell pathology, Female, Humans, Kidney Neoplasms genetics, Kidney Neoplasms pathology, Male, MicroRNAs genetics, Middle Aged, Transfection, Carcinoma, Renal Cell blood, Extracellular Vesicles metabolism, Kidney Neoplasms blood, MicroRNAs blood
- Abstract
Aim: To identify novel diagnostic markers for renal cell carcinoma (RCC), we analyzed miRNAs in serum extracellular vesicles (EVs)., Materials and Methods: EVs were purified from serum of healthy controls and patients with localized and advanced RCC using T-cell immunoglobulin domain and mucin domain-containing protein 4 conjugated to magnetic beads. miRNA profiling of EVs was conducted by microarray analysis. miRNA expression was examined by quantitative reverse transcription-polymerase chain reaction. Lastly, proteomic analysis of RCC cells transfected with a miRNA inhibitor was performed to identify its potential targets., Results: Microarray analysis revealed that nine miRNAs were increased by more than 1.5-fold in EVs from patients with RCC. Among them, miRNA-4525 was significantly elevated; miRNA-4525 expression was higher in RCC tissue than in the adjacent normal tissue. Proteomic analysis identified alpha fetoprotein and albumin as its potential targets., Conclusion: These findings suggest the potential of miRNA-4525 in serum EVs as a novel biomarker for advanced RCC., (Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2021
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47. Spontaneous healing of rectal penetration by SpaceOAR ® hydrogel insertion during permanent iodine-125 implant brachytherapy: A case report.
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Iinuma K, Mizutani K, Kato T, Nakane K, Tanaka H, Nakano M, Matsuo M, and Koie T
- Abstract
Radiation therapy with permanent iodine-125 implant brachytherapy is well established for curable prostate cancer. To maximize the therapeutic potential of brachytherapy, delivered radiation doses have been increased accompanied by refined intraoperative procedures of radioactive seeds placement. However, dose escalation is a double-edged sword in prostate brachytherapy; it could induce rectal toxicity, yet is successful in curable treatment in prostate cancer. To reduce irradiation of the rectum, the SpaceOAR
® system has been used to inject synthetic polyethylene glycol hydrogel between the prostate and the rectum. The present report describes a case of spontaneous healing of rectal penetration associated with SpaceOAR® hydrogel., (Copyright: © Iinuma et al.)- Published
- 2019
- Full Text
- View/download PDF
48. Upregulation of miR-130b Contributes to Risk of Poor Prognosis and Racial Disparity in African-American Prostate Cancer.
- Author
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Hashimoto Y, Shiina M, Dasgupta P, Kulkarni P, Kato T, Wong RK, Tanaka Y, Shahryari V, Maekawa S, Yamamura S, Saini S, Deng G, Tabatabai ZL, Majid S, and Dahiya R
- Subjects
- Adenocarcinoma diagnosis, Adenocarcinoma ethnology, Adenocarcinoma genetics, Adenocarcinoma mortality, Adult, Black or African American, Aged, Aged, 80 and over, Genetic Predisposition to Disease, Humans, Male, Middle Aged, PC-3 Cells, Prognosis, Prostatic Neoplasms mortality, Race Factors, Risk Factors, Survival Analysis, Tumor Cells, Cultured, Up-Regulation genetics, Health Status Disparities, MicroRNAs genetics, Prostatic Neoplasms diagnosis, Prostatic Neoplasms ethnology, Prostatic Neoplasms genetics
- Abstract
Prostate cancer incidence and mortality rates are higher in African-American (AA) than in European-American (EA) men. The main objective of this study was to elucidate the role of miR-130b as a contributor to prostate cancer health disparity in AA patients. We also determined whether miR-130b is a prognostic biomarker and a new therapeutic candidate for AA prostate cancer. A comprehensive approach of using cell lines, tissue samples, and the TCGA database was employed. We performed a series of functional assays such as cell proliferation, migration, invasion, RT2-PCR array, qRT-PCR, cell cycle, luciferase reporter, immunoblot, and IHC. Various statistical approaches such as Kaplan-Meier, uni-, and multivariate analyses were utilized to determine the clinical significance of miR-130b. Our results showed that elevated levels of miR-130b correlated with race disparity and PSA levels/failure and acted as an independent prognostic biomarker for AA patients. Two tumor suppressor genes, CDKN1B and FHIT , were validated as direct functional targets of miR-130b. We also found race-specific cell-cycle pathway activation in AA patients with prostate cancer. Functionally, miR-130b inhibition reduced cell proliferation, colony formation, migration/invasion, and induced cell-cycle arrest. Inhibition of miR-130b modulated critical prostate cancer-related biological pathways in AA compared with EA prostate cancer patients. In conclusion, attenuation of miR-130b expression has tumor suppressor effects in AA prostate cancer. miR-130b is a significant contributor to prostate cancer racial disparity as its overexpression is a risk factor for poor prognosis in AA patients with prostate cancer. Thus, regulation of miR-130b may provide a novel therapeutic approach for the management of prostate cancer in AA patients., (©2019 American Association for Cancer Research.)
- Published
- 2019
- Full Text
- View/download PDF
49. Retracted: Transplantation of brown adipose tissue inhibits atherosclerosis in apoE-/- mice: contribution of the activated FGF-21-adiponectin axis.
- Author
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Kikai M, Yamada H, Wakana N, Terada K, Yamamoto K, Wada N, Motoyama S, Saburi M, Sugimoto T, Irie D, Kato T, Kawahito H, Ogata T, and Matoba S
- Published
- 2018
- Full Text
- View/download PDF
50. Exercise-induced Atrioventricular Block with Coronary Artery Stenosis that Appeared Five Years after Bypass Surgery.
- Author
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Yamazaki S, Kato T, Ushimaru S, Yokoi H, and Mani H
- Subjects
- Aged, Atrioventricular Block diagnostic imaging, Coronary Stenosis diagnostic imaging, Coronary Stenosis surgery, Humans, Male, Syncope diagnostic imaging, Syncope physiopathology, Treatment Outcome, Atrioventricular Block etiology, Atrioventricular Block surgery, Coronary Artery Bypass adverse effects, Coronary Artery Disease surgery, Coronary Stenosis etiology, Exercise physiology
- Abstract
A 68-year-old man with a history of coronary artery bypass surgery was referred to our hospital because of pre-syncope on effort. During a treadmill exercise electrocardiogram test, the patient developed advanced atrioventricular block associated with dizziness. Coronary angiography revealed significant stenosis of the right coronary artery, which had not existed at the time of the bypass surgery. We implanted drug-eluting stents in the stenotic lesion, and an exercise test showed resolution of the atrioventricular block. Exercise-induced atrioventricular block is rare, and it is necessary to distinguish it from ischemic heart disease, especially in patients with a history of coronary artery disease.
- Published
- 2018
- Full Text
- View/download PDF
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