1. Biocatalysis synthesis of rutin esters of α-linolenic acid.
- Author
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WANG Lian, ZHENG Ming-ming, HUANG Qing, GUO Ping-mei, ZHENG Chang, and HUANG Feng-hong
- Subjects
LINOLENIC acids ,BIOCATALYSIS ,BIOSYNTHESIS ,RUTIN ,ESTERS analysis ,ESTERIFICATION - Abstract
An enzymatic synthesis process for a new functional lipid prepared with rutin and a - linolenic acid was established using commercially immobilized lipase from Candia antarctica ( Novozym435 ) as biocatalyst. Re-sponse surface methodology (RSM) was adopted to evaluate the effects of reaction parameters on esterification rate. The optimum process parameters were as follows: 17. 9mg/mL Novozym 435, 17. 3mmolL rutin concentration, 14.5 molar ratio of rutin to α- linolenic, 60 h for reaction in acetone, with esterification rate of 92. 6%. After purification, the purity of products was reached up to 95. 0%. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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