1. Proteomics and Gastrointestinal Dynamic Models: 2 Possible Allies
- Author
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Vincenzo Fierro, Rocco Luigi Valluzzi, Maurizio Mennini, and Alessandro Fiocchi
- Subjects
Proteomics ,03 medical and health sciences ,0302 clinical medicine ,animals ,cattle ,female ,goats ,humans ,infant ,milk, human ,proteolysis ,infant formula ,proteomics ,030225 pediatrics ,Medicine ,Animals ,Humans ,human ,milk ,Milk, Human ,business.industry ,Goats ,Gastroenterology ,Original Articles: Nutrition ,Infant ,DIAAS ,Data science ,Infant Formula ,Dynamic models ,protein digestion kinetics ,Pediatrics, Perinatology and Child Health ,Proteolysis ,030211 gastroenterology & hepatology ,Cattle ,Female ,true ileal protein digestibility ,business ,digestible indispensable amino acid score - Abstract
Objective: The aim of this study was to determine the kinetics of true ileal protein digestion and digestible indispensable amino acid score (DIAAS) of a goat milk-based infant formula (GIF), a cow milk-based infant formula (CIF), and human milk (HM). Methods: The GIF, CIF, and HM were investigated in an in vitro gastrointestinal model simulating infant conditions. Digested compounds were dialyzed from the intestinal compartment as bioaccessible fraction. Dialysate was collected in 15 to 60-minute periods for 4 hours. True ileal protein digestibility and DIAAS were determined as bioaccessible nitrogen (N) and amino acids. Results: N bioaccessibility from the GIF showed similar kinetics to that of HM. The CIF showed a delay in N bioaccessibility versus the GIF and HM. In the 1st hour of digestion, N bioaccessibility was 19.9% ± 3.5% and 23.3% ± 1.3% for the GIF and HM, respectively, and 11.2% ± 0.6% for CIF (P
- Published
- 2018