1. An in vitro bacterial adhesion assessment of surface-modified medical-grade PVC
- Author
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Igor Novák, Ita Junkar, Vladimír Sedlařík, Marián Lehocký, Petr Saha, Ivan Chodák, Alenka Vesel, and Ahmad Asadinezhad
- Subjects
Staphylococcus aureus ,Surface Properties ,Biocompatible Materials ,Bacterial Adhesion ,Chitosan ,chemistry.chemical_compound ,Benzalkonium chloride ,Colloid and Surface Chemistry ,antibacterial activity ,Anti-Infective Agents ,nitrogen-plasma ,Polymer chemistry ,Escherichia coli ,medicine ,ozone-treatment ,Physical and Theoretical Chemistry ,polymer surfaces ,Polyvinyl Chloride ,poly(vinyl chloride) ,polyvinylchloride ,Chemistry ,plasma glow-discharge ,chlorhexidine ,Chlorhexidine ,Biofilm ,Surfaces and Interfaces ,General Medicine ,Adhesion ,bacterial adhesion ,Polyvinyl chloride ,plasma treatment ,Models, Chemical ,Propylene Glycols ,Biofilms ,Drug Design ,Wettability ,Surface modification ,films ,chitosan ,Benzalkonium Compounds ,Antibacterial activity ,surface modification ,oxygen ,Biotechnology ,medicine.drug ,Nuclear chemistry - Abstract
Medical-grade polyvinyl chloride was surface modified by a multistep physicochemical approach to improve bacterial adhesion prevention properties. This was fulfilled via surface activation by diffuse coplanar surface barrier discharge plasma followed by radical graft copolymerization of acrylic acid through surface-initiated pathway to render a structured high density brush. Three known antibacterial agents, bronopol, benzalkonium chloride, and chlorhexidine, were then individually coated onto functionalized surface to induce biological properties. Various modern surface probe techniques were employed to explore the effects of the modification steps. In vitro bacterial adhesion and biofilm formation assay was performed. Escherichia coli strain was found to be more susceptible to modifications rather than Staphylococcus aureus as up to 85% reduction in adherence degree of the former was observed upon treating with above antibacterial agents, while only chlorhexidine could retard the adhesion of the latter by 50%. Also, plasma treated and graft copolymerized samples were remarkably effective to diminish the adherence of E. coli. (C) 2010 Elsevier B.V. All rights reserved.
- Published
- 2010
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