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Coffee constitutes a plant product high in economic value. Extremely abundant throughout Indonesia, different varieties can be found in each region. Coffee leaves represent a by-product of coffee production which are disposed of without being subjected to further processing. One advantage of coffee leaf waste is that it can be made into coffee leaf tea, to prevent various diseases. The caffeine and tannin content can be optimized by temperature and brewing time. Research on coffee leaf tea remains limited, with little study of Liberica coffee leaf tea. This investigation was to determine the optimal brewing time and temperature of Robusta (Coffea canephora) and Liberica (Coffea liberica) coffee leaf tea. This research used the Response Surface Methodology (RSM) method with Central Composite Design (CCD). Data analysis using RSM method was conducted incorporating two factors. The first factor was the brewing temperature with a minimum value of 91 °C and a maximum value of 99 °C. The second factor was brewing time with minimum and maximum values of three and seven minutes respectively. Identifying the optimal treatment was based on two factors, caffeine and tannin content. Optimized processes were applied to conduct organoleptic tests on 110 untrained panelists using the Rate-All-That-Apply (RATA) method to highlight the majority attributes experienced by the panelists. The optimal brewing temperature and duration for robusta coffee leaf tea were 93.43 °C and 4.80 minutes which produced caffeine and tannin levels of 74.90 mg.100mL-1 and 293.01 µg.g-1 respectively. In contrast, in the case of Liberica coffee leaf tea, the optimal brewing conditions comprised a temperature of 91.65 °C and duration of 4.84 minutes which produced caffeine and tannin levels of 72.52 mg.100mL-1 and 415.87 µg.g-1. Results of sensory analysis showed that the majority produced five significant characteristics, namely: sweet flavor, fire flavor, sweet taste, bitter taste and astringent mouthfeel.

Baltazar AMP, Buot JrIE. 2019. Short Communication: Leaf architectural analysis of taxonomically confusing coffee species: Coffea liberica and Coffea liberica var. dewevrei. Biodiversitas 20: 1560-1567. Coffee is considered as one of the most important crops. The Philippines is known to produce four coffee varieties namely: Arabica (Coffea arabica), Robusta (Coffea canephora), Excelsa (Coffea liberica var. dewevrei) and Liberica (Coffea liberica). Further, the taxonomy of C. liberica and C. liberica var. dewevrei is still unclear. Since its earliest publication, the relationship of the two taxa have been confusing many taxonomists and also farmers. The status of the two confusing taxa are still under discussions due to contradicting evidence inferred from morphological and molecular data. The two taxa have been claimed as separated species but the markers are considered inconsistent in the field. This study has tried to examine the leaf architectural characters of both species as these have been known to be genetically fixed. Leaf samples of each species including C. canephora (outgroup) were collected and examined. Results showed that the two taxa were found to be different in laminar size, domatia distribution, major secondary attachment, and intercostal tertiary veins. Cluster analysis revealed that they are grouped separately. However, the rank of C. liberica var. dewevrei cannot be decided yet until further taxonomic study, particularly on the anatomy and distribution pattern of domatia, is completed.