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The wellbeing of horses is very important to their care takers, trainers, veterinarians, and owners. This thesis describes the development of a non-invasive image processing technique that allows for automatic detection and tracking of horse head and ear motion, respectively, in videos or camera feed, both of which may provide indications of horse pain, stress, or well-being. The algorithm developed here can automatically detect and track head motion and ear motion, respectively, in videos of a standing horse. Results demonstrating the technique for nine different horses are presented, where the data from the algorithm is utilized to plot absolute motion vs. time, velocity vs. time, and acceleration vs. time for the head and ear motion, respectively, of a variety of horses and ponies. Two-dimensional plotting of x and y motion over time is also presented. Additionally, results of pilot work in eye detection in light colored horses is also presented. Detection of pain in horses is particularly difficult because they are prey animals and have mechanisms to disguise their pain, and these instincts may be particularly strong in the presence of an unknown human, such as a veterinarian. Current state-of-the art for detecting pain in horses primarily involves invasive methods, such as heart rate monitors around the body, drawing blood for cortisol levels, and pressing on painful areas to elicit a response, although some work has been done for humans to sort and score photographs subjectively in terms of a "horse grimace scale." The algorithms developed in this thesis are the first that the author is aware for exploiting proven image processing approaches from other applications for development of an automatic tool for detection and tracking of horse facial indicators. The algorithms were done in common open source programs Python and OpenCV, and standard image processing approaches including Canny Edge detection Hue, Saturation, Value color filtering, and contour tracking were utilized in algorithm development. The work in this thesis provides the foundational development of a non -invasive and automatic detection and tracking program for horse head and ear motion, including demonstration of the viability of this approach using videos of standing horses. This approach lays the groundwork for robust tool development for monitoring horses non-invasively and without the required presence of humans in such applications as post-operative monitoring, foaling, evaluation of performance horses in competition and/or training, as well as for providing data for research on animal welfare, among other scenarios. MS There are many things that cause pain in horses, including improper saddle fit, inadequate care, laminitis, lameness, surgery, and colic, among others.The well-being of horses is very important to their care takers, trainers, veterinarians, and owners. Monitoring the well-being of horses is particularly important in many scenarios including post-operative monitoring, therapeutic riding programs, racing, dressage, and rodeo events, among numerous other activities. This thesis describes the development of a computer-based image processing technique for automatic detection and tracking of both horse head and ear motion, respectively, in videos of standing horses. The techniques developed here allow for the collection of data on head and ear motion over time, facilitating analysis of these motions that may provide reliable indicators of horse pain, stress, or well-being. Knowing if a horse is in pain is difficult because horses are prey animals that have mechanisms in place that minimize the display of pain so that they do not become easy targets for predators. Computer vision systems, like the one developed here, may be well suited to detect subtle changes in horse behavior for detecting distress in horses. The ability to remotely and automatically monitor horse well-being by exploiting computer-based image-processing techniques will create significant opportunities to improve the welfare of horses. The work presented here looks at the first use of image-processing approaches to detect and track facial features of standing horses in videos to help facilitate the development of automatic pain and stress detection in videos and camera feeds for owners, veterinarians, and horse-related organizations, among others.

Simple Summary Acute laminitis is a common equine disease characterized by intense foot pain. This work aimed to investigate whether the Horse Grimace Scale (HGS), a facial-expression-based pain coding system, can be usefully applied to assess pain associated with acute laminitis in horses at rest. Ten horses, referred as acute laminitis cases with no prior treatment, were assessed at the admission and at seven days after the initial evaluation and treatment. The authors found that the Horse Grimace Scale is a potentially effective method to assess pain associated with acute laminitis in horses at rest, as horses showing high HGS scores also exhibited higher Obel scores, and veterinarians classified them in a more severe painful state. Abstract Acute laminitis is a common equine disease characterized by intense foot pain, both acutely and chronically. The Obel grading system is the most widely accepted method for describing the severity of laminitis by equine practitioners, however this method requires movement (walk and trot) of the horse, causing further intense pain. The recently developed Horse Grimace Scale (HGS), a facial-expression-based pain coding system, may offer a more effective means of assessing the pain associated with acute laminitis. The aims of this study were: to investigate whether HGS can be usefully applied to assess pain associated with acute laminitis in horses at rest, and to examine if scoring HGS using videos produced similar results as those obtained from still images. Ten horses, referred as acute laminitis cases with no prior treatment, were included in the study. Each horse was assessed using the Obel and HGS (from images and videos) scales: at the admission (before any treatment) and at seven days after the initial evaluation and treatment. The results of this study suggest that HGS is a potentially effective method to assess pain associated with acute laminitis in horses at rest, as horses showing high HGS scores also exhibited higher Obel scores and veterinarians classified them in a more severe painful state. Furthermore, the inter-observer reliability of the HGS total score was good for both still images and video evaluation. There was no significant difference in HGS total scores between the still images and videos, suggesting that there is a possibility of applying the HGS in clinical practice, by observing the horse for a short time. However, further validation studies are needed prior to applying the HGS in a clinical setting.