1. Protective effects of selenium in tacrolimus-induced lung toxicity: potential role of heme oxygenase 1
- Author
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Nashwa El-Tahway, Hanaa Mohamed Khalaf, Ahlam M. Abdalla, and Salwa A. Ibrahim
- Subjects
Male ,NF-E2-Related Factor 2 ,Physiology ,Acute Lung Injury ,chemistry.chemical_element ,Pharmacology ,Protective Agents ,Antioxidants ,Tacrolimus ,Hemeoxygenase 1 ,Selenium ,Malondialdehyde ,Physiology (medical) ,Animals ,Medicine ,Drug Interactions ,Rats, Wistar ,Superoxide Dismutase ,Lung toxicity ,business.industry ,NF-kappa B ,General Medicine ,Interleukin-10 ,Rats ,Heme oxygenase ,Oxidative Stress ,chemistry ,Heme Oxygenase (Decyclizing) ,business ,Immunosuppressive Agents - Abstract
The present study aimed to evaluate the protective effects of selenium (Sel) administration against tacrolimus (Tac) – induced lung toxicity and to assess the relation between heme oxygenase 1 (HO-1) and these effects. The study was conducted on 36 Wistar male albino rats equally divided into four groups: (i) normal control; (ii) Sel (0.1 mg/kg per day p.o. for four weeks); (iii) TAC 3 mg/mL as single oral dose on 27th day; and (iv) Tac + Sel. Lung tissues, lung homogenate, and bronchoalveolar lavage of the sacrificed animals were investigated biochemically and histopathologically, by immunohistochemistry or by PCR. The Tac group showed significantly lower expression of HO-1. Administration of Sel was associated with increased HO-1 expression. Oxidative (malondialdehyde, reduced glutathione, superoxide dismutase, myeloperoxidase, and glutathione peroxidase activity) and nitrosative stress (nitric oxide) markers and markers of inflammation (interleukin 1β (IL-1β), IL-6, and IL-10) showed changes corresponding to HO-1 levels in rat groups. Tac group showed the highest expression of caspase-3. Sel exerted a protective role against Tac-induced lung toxicity.
- Published
- 2021