1. Six weeks of high-load resistance and low-load blood flow restricted training increase Na/K-ATPase sub-units α2 and β1 equally, but does not alter ClC-1 abundance in untrained human skeletal muscle
- Author
-
Wang, Jakob, Rindom, Emil, Groennebaek, Thomas, Sieljacks, Peter, Jakobsgaard, Jesper Emil, Farup, Jean, Vissing, Kristian, Pedersen, Thomas Holm, and de Paoli, Frank Vincenzo
- Subjects
Muscle excitability ,Physiology ,Cell Biology ,Function ,Cl conductance ,Exercise ,Biochemistry - Abstract
Contractile function of skeletal muscle relies on the ability of muscle fibers to trigger and propagate action potentials (APs). These electrical signals are created by transmembrane ion transport through ion channels and membrane transporter systems. In this regard, the Cl - ion channel 1 (ClC-1) and the Na +/K --ATPase (NKA) are central for maintaining ion homeostasis across the sarcolemma during intense contractile activity. Therefore, this randomized controlled trial aimed to investigate the changes in ClC-1 and specific NKA subunit isoform expression in response to six weeks (18 training sessions) of high-load resistance exercise (HLRE) and low-load blood flow restricted resistance exercise (BFRRE), respectively. HLRE was conducted as 4 sets of 12 repetitions of knee extensions performed at 70% of 1 repetition maximum (RM), while BFRRE was conducted as 4 sets of knee extensions at 30% of 1RM performed to volitional fatigue. Furthermore, the potential associations between protein expression and contractile performance were investigated. We show that muscle ClC-1 abundance was not affected by either exercise modality, whereas NKA subunit isoforms [Formula: see text] 2 and [Formula: see text] 1 increased equally by appx. 80-90% with BFRRE (p
- Published
- 2023
- Full Text
- View/download PDF