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Milk and dairy products are an important part of the human diet for numerous reasons. Goat milk is higher in the content of elements such as K and Ca. Nowadays, consumption of goat milk and goat dairy products increases because of better digestion and therapeutic value. However, goat milk can contain heavy metals and trace elements as well which can harm human health. The content of 22 elements in sixty-nine samples of goat milk, whey, three types of cheese, and yogurt from the ecological farm in region Orava and conventional farm in region Stredné Považie in Slovakia was measured in this study. The highest concentration of four elements Ca, K, Mg, and Na in samples of goat products was found. In our study, significant statistical differences (P < 0.05) were found only in levels of K, Ca, Li and Na when comparing milk samples from organic and conventional farm. Concentrations of K (1260.50 mg/kg), Li (0.02 mg/kg) and Na (293.46 mg/kg) were higher in samples from ecological farm, while concentration of Ca (1344.65 mg/kg) was higher in samples from conventional farm. Toxic elements were present in trace amounts or under the limit of detection. The consumption of goat milk and goat dairy products from monitored farms can be considered as safe and beneficial for human health regardless of the way of farming. According to the highest level of strontium in hard ripening cheese, frequent regular consumption should be considered for children as a preventive measure for development of bone health., Mlieko a mliečne výrobky sú dôležitou zložkou v strave ľudí z viacerých dôvodov. Všetky typy mlieka sa skladajú z tých istých zložiek, ale ich množstvo je rozdielne. Kozie mlieko obsahuje vyššie množstvo draslíka a vápnika. V súčasnosti konzumácia ovčích mliečnych výrobkov stúpa kvôli ich ľahšej stráviteľnosti a terapeutickej hodnote, ktorá im je pripisovaná. Kozie mlieko a výrobky z neho avšak môžu v dôsledku environmentálneho znečistenia predstavovať aj zdroj ťažkých kovov, ktoré následne môžu pôsobiť nepriaznivo na ľudské zdravie. Obsah 22 prvkov v 69 vzorkách kozieho mlieka a výrobkov z neho bol stanovovaný v tejto štúdii. Vzorky pochádzali z ekologickej farmy z regiónu Orava a konvenčnej farmy, ktorá sa nachádza v regióne Stredné Považie. Najvyššie koncentrácie vo vzorkách kozích mliečnych produktov dosahovali prvky vápnik, draslík, horčík a sodík. Pri porovnaní vzoriek kozieho mlieka z ekologickej a konvenčnej farmy boli nájdené štatistické rozdiely (P < 0,05) len pri obsahu prvkov K, Ca, Li a Na. Vyššie koncentrácie prvkov draslíka (1260,50 mg/kg), lítia (0,02 mg/kg) a sodíka (293,46 mg/kg) sa našli vo vzorkách mlieka pochádzajúcich z ekologickej farmy, kým vyššia priemerná koncentrácia vápnika (1344,65 mg/kg) sa nachádzala vo vzorkách mlieka z konvenčnej farmy. Toxické prvky boli stanovené v stopových množstvách, alebo pod limitom detekcie. Na základe výsledkov môžeme považovať kozie mlieko a výrobky z neho z oboch fariem za bezpečné pre ľudskú spotrebu s možným priaznivým účinkom na zdravie bez ohľadu na spôsob hospodárenia. Častá a pravidelná konzumácia tvrdého zrejúceho syra malými deťmi je na zváženie, ako preventívne opatrenie podpory vývoja kostného zdravia, vzhľadom na najvyšší obsah stôp stroncia.

Metabolic syndrome has become a major health hazard of the modern world. Studies investigating the effects of traditional fermented foods on metabolic syndrome are limited. We hypothesized that regular kefir consumption could improve the anthropometrical measurements, glycemic control, lipid profile, blood pressure, and inflammatory status in patients with metabolic syndrome. Sixty-two participants were randomly assigned to receive either 180 mL/d probiotic kefir or unfermented milk for 12 weeks. Dietary intake, anthropometrical measurements, biochemical status, and blood pressure were assessed at baseline and the end of weeks 4, 8, and 12. Serum apolipoprotein A1 concentration increased by 3.4% in the kefir group, whereas it decreased by 2.4% in the milk group in 12 weeks (P = .03). A subgroup analysis for participants with low-density lipoprotein cholesterol (LDL-C) levels >130 mg/dL showed that serum LDL-C and apolipoprotein B concentrations (7.6% and 5.4%, respectively) significantly decreased with kefir consumption compared with the baseline values at the 12th week (P < .05), but not compared with milk consumption (P > .05). Both milk and kefir consumption was associated with lower systolic and diastolic blood pressure compared with the baseline (P < .05). The 12-weeks of kefir administration also decreased serum tumor necrosis factor-?, interleukin 6, interleukin 10, interferon-gamma, and homocysteine concentrations significantly (P < .05). In conclusion, regular dairy consumption as part of a well-balanced diet can provide favorable effects in the management of metabolic syndrome, and probiotic kefir may deserve a special interest among dairy products. This trial was registered at clinicaltrials.gov (NCT03966846). © 2022, This work was supported by the Teaching Staff Training Program of the Turkish Council of Higher Education .

Artisanal food industry from agricultural and livestock crops have major potential on improving local community welfare and even contributing to sustainability if they are processed, produced and marketed properly. Agricultural and dairy products can be processed into a more diverse range of products that can attract wider consumers, particularly burgeoning urban middle-class. However, studies on such industry, particularly those in low and lower-middle income countries, are disproportionally sparse in the literature. In this study, we examine economic viability of product diversification of swamp buffalo milk-based artisanal confectionery product named gulo puan, which is exclusively produced in Pampangan sub-district, Ogan Komering Ilir regency, South Sumatra, Indonesia. To improve its marketability, the diversification of gulo puan into chocolate bar-like products was proposed. Financial feasibility analyses with the investment criteria, namely NPV, Net B/C, IRR, payback period, and sensitivity analysis were conducted and show that the proposed implementation is viable, robust, and realistic. However, declining number of swamp buffaloes in recent years due to farming mechanization, as well as lack of infrastructure for supply chain, remain as challenges in the future that need to be addressed by stakeholders and policymakers.

Milk fat is an important nutrient for young animals and humans, and its composition [fatty acids (FA)] may vary broadly. The objective of this study was to describe the development of global FA models for high-throughput Fourier transform infrared milk analysers. The developed models for major FA, FA grouped by chain length, by degree of unsaturation and by their origin, showed good accuracy, repeatability and correlation to gas chromatography. Moderate performance was obtained for low concentration FA (short chain, polyunsaturated and trans). Possible practical applications could be to enhance dairy products, optimise milk processing and/or optimise dairy cow performance through improved feeding and management.

This study aimed to determine and compare aluminum (Al), arsenic (As), cadmium (Cd), and lead (Pb) levels in conventional and organic milk, white cheese, and butter samples which are sold in the markets of Turkey. Within the scope of the study, totally 72 samples (36 for organic, and 36 for conventional) were collected, and analyzed between March 2010 and February 2011. After the samples were burned by microwave burning, element measurements were performed on atomic absorption spectrophotometer (AAS) device. As a result of the study, on the 49 samples (29 conventional, 20 organic) of the 72 collected product, element levels were determined above the limit of detection (LOD) (0.02 ppm). Pb and Al were the most common elements above the LOD. However, milk samples with a Pb level above the LOD still remained below the limit reported by the Turkish Food Codex Regulation for Contaminants. Since Turkish Food Codex Regulation does not have legal limits for Al, As, Cd and Pb for dairy products (cheese and butter), it was accepted as safe in terms of Al, As, Cd and Pb according to food limits in Turkish Food Codex and ADI values of FAO / WHO.

Coalho cheese is a typical Northeastern Brazilian food whose production and consumption has expanded considerably to other regions of the country. Despite its cultural and socioeconomic relevance, coalho cheese still has a negative reputation due to problems in its physicochemical and microbiological quality standards, especially when it is produced artisanally. For this reason, many researchers have been looking for alternatives to improve the quality and the notoriety of this product, such as the application of probiotic strains to inhibit pathogens. However, in addition to the research related to the desired probiotic properties and the safety of strains for food application, it is important to know the possible changes in the food matrix caused by this intervention. In this context, the aim of this study was to evaluate the effect of the incorporation of probiotic strains of Enterococcus faecium on the physicochemical and sensory characteristics of coalho cheese. The cheeses were made with pasteurized cow milk and probiotic strains of E. faecium were added during production. Cheeses were also made without adding the strains, and considered as control cheeses. The final products were evaluated for moisture, pH, titratable acidity, total carbohydrates, proteins, lipids, ash, color, organic acid profile, and sensory attributes. In addition, viable lactic acid bacteria were also assessed. Coalho cheeses containing E. faecium showed higher titratable acidity and lower pH. In addition, there was a reduction in the carbohydrate content, which was attributed to lactose degradation. The organic acid profile also varied, as highlighted by the increase in the concentration of lactic acid by a factor of 3.5, and by the absence of citric acid as a possible result of the metabolism of E. faecium in the production of aromatic compounds. There was no variation in the color or sensory acceptance of the cheeses. Therefore, the application of probiotic strains of E. faecium in coalho cheese production changes some physicochemical characteristics while maintaining essential properties, such as the color and sensory acceptability of the final product. O queijo coalho é um queijo típico do Nordeste do Brasil, cuja produção e consumo se expandiram consideravelmente para outras regiões do país. Apesar de sua relevância cultural e socioeconômica, o queijo coalho apresenta uma reputação negativa, devido a problemas em seus padrões de qualidade físicoquímicos e microbiológicos, principalmente quando é produzido artesanalmente. Por esse motivo, muitos pesquisadores têm buscado alternativas para melhoria da qualidade e notabilidade desse produto, como a aplicação de cepas probióticas que inibem patógenos. No entanto, além da investigação relacionada às propriedades probióticas desejadas e de segurança das cepas para aplicação em alimentos, é importante conhecer as possíveis alterações na matriz alimentícia em virtude dessa adição. Nesse contexto, o objetivo deste estudo foi avaliar o efeito da incorporação de cepas probióticas de Enterococcus faecium nas características físico-químicas e sensoriais de queijo coalho. Os queijos foram elaborados com leite de vaca pasteurizado e adicionados de cepas probióticas de E. faecium durante a produção. Também foram elaborados queijos sem adição das cepas, considerados queijos controle. Os produtos finais foram avaliados quanto a umidade, pH, acidez titulável, carboidratos totais, proteínas, lipídios, cinzas, cor, perfil de ácidos orgânicos e atributos sensoriais. Além disso, procedeu-se a contagem de bactérias ácido lácticas viáveis. Os queijos coalho adicionados de E. faecium apresentaram maior acidez titulável e menor pH. Além disso, houve uma redução no teor de carboidratos, atribuída à degradação da lactose. O. perfil de ácidos orgânicos também variou, destacando-se pelo aumento da concentração de ácido láctico em um fator de 3,5 e pela ausência de ácido cítrico, como possível resultado do metabolismo de E. faecium na produção de compostos aromáticos. Não houve variação na cor nem na aceitação sensorial dos queijos. Portanto, a aplicação das cepas probióticas de E. faecium deste estudo na produção de queijos coalho, altera algumas características físico-químicas, mantendo propriedades importantes, tais como cor e aceitabilidade sensorial do produto final.

Proving that an endpoint (e.g. URL, telephone number, etc.) is controlled by a subject is crucial in many applications. In the web, this is witnessed by the widespread adoption of HTTPS. In centralized architectures, this task is usually carried out by trusted certification authorities (CAs). In decentralized applications, for example based on blockchains, or for self-sovereign identity management (SSI), it would be desirable to perform these checks in a decentralized way, relying on the collective behavior of a society of individuals rather than on a single trusted entity. In any case, the result should be a widely usable certificate, as in the centralized CA case. In this paper, we show two blockchain-based methods to prove the association between a subject and an endpoint in a decentralized manner. Our methods are compatible with a wide variety of endpoints and contribute to fill the gap of the current SSI approaches with respect to decentralization. We analyze the security of our proposal and provide a proof-of-concept implementation. We also evaluate performances, costs, and compatibility with current standardization efforts about SSI.

The increased interest in foods which improve human health and nutrition has led to the need for academia and food industry to invest in the development of innovative and functional ice cream. Since ice cream is the most popular frozen dairy product, it shows good potential to help people improve their diets by reducing the intake of certain nutrients associated with the increased risk of obesity and other related diseases. Moreover, it supplies beneficial and essential components. However, the reduction or removal of common ice cream ingredients or the addition of uncommon ingredients to the standard formulation must not spoil the sensory characteristics and storage stability of ice cream. Hence, to keep the appeal of ice cream high, the food industry is subjected to constant challenges, since functional ice cream must be tasty as well as beneficial. The review focuses on the healthy aspects and sensory implications of ice cream functionalization. In the first part, different replacing strategies such as the substitution of fat and sugar are discussed. In the second part, ice cream enriched with functional ingredients is debated.

Green soybean (Glycine max L.) pods (GSP) are agro-industrial waste from the production of frozen green soybean and milk. These pods contain natural antioxidants and various bioactive compounds that are still underutilized. Polyphenols and flavonoids in GSP were extracted by ultrasound techniques and used in antioxidant fortification of green soybean milk. The ultrasound extraction that yielded the highest total polyphenol content was 50% amplitude for 10 min, whereas maximum flavonoids content was obtained at 50% amplitude for 15 min. Radical scavenging activity assayed by 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH) and ferric reducing antioxidant power (FRAP) methods did not differ significantly (p>0.05) between the two conditions. Response surface methodology was applied to analyze an optimum ultrasonic-assisted extraction (UAE) condition of these variables. The highest desirability was found to be 50% amplitude with extraction time of 12.5 min. Fortification of the GSP extracts (1-3% v/v) in green soybean milk resulted in higher levels of bioactive compounds and antioxidant activity in a dose-dependent manner. Procyanidins were found to be the main polyphenols in dried GSP which were present at the concentration of 104.1 ± 2.1 mg/g dry sample, respectively. Addition of GSP extracts obtained by using an ultrasound technique to green soybean milk increased its bioactive compound content especially procyanidins as well as its antioxidant activity.

Betacyanins are betalain derivatives showing a red-violet color suitable as a natural food colorant. An aqueous extract rich in betacyanin pigments and phenolic compounds from Alternanthera brasiliana was characterized using a high-performance liquid chromatography system with diode array and electrospray mass spectrometry detection. The major betacyanins (amaranthine, isoamaranthine, betanin and isobetanin, celosianin II and isocelosianin II) were identified and quantified. Eleven peaks were analyzed for phenolic compounds grouped into hydroxycinnamic acids and flavones. Different plant extract concentrations were added to freshly prepared set type yogurts (350, 700, and 1400 μL/80 g yogurt). Color properties in CIELAB space, pH, total phenolic content, and ferric reducing antioxidant power were analyzed on yogurts during storage time. The evolution of pigments and polyphenolic compounds during cold storage was studied by HPLC-ESI-QTOF-MS screening. The extracted color showed to be stable at the yogurt pH range. When added to yogurts, a natural berry-like color was achieved. These results provide a natural plant pigment-based alternative for application in food products, being the first time that A. brasiliana is used as a colorant in a dairy product. Besides color, polyphenols input turns Alternanthera brasiliana extract into a desirable functional food ingredient., The contribution of project AGL2016-77056-R (AEI/FEDER, UE) from the Spanish MINECO, is acknowledged.

Songklanakarin Journal of Science an Technology (SJST), 44, 3, 619-626

This study aimed to highlight the effect of cow milk addition (0, 15, 30, and 45%) on Brazilian semi-hard buffalo cheese. Four formulations with different buffalo and cow milk ratios (v/v) were prepared: CC00 (100:00%); CC15 (85:15%); CC30 (70:30%); and CC45 (55:45%). The CC00 presented the highest moisture, fat in dry matter, and salt contents, while CC45 exhibited the highest protein and lowest pH values. CC45 presented the highest flavor, texture, overall acceptability, and purchase intention. Therefore, cow milk addition at 45% is an alternative to improve Brazilian semi-hard buffalo cheese's acceptability and purchase intention and, consequently, a market opportunity for the diversification of buffalo dairy products.

Background & objectives Food-based dietary guidelines (FBDGs) have been developed to promote healthy diets and prevent chronic diseases. However, the methodological quality of Spanish FBDGs has not been systematically assessed yet. The objective of this review is to identify and assess the methodological quality of Spanish FBDGs, as well as to describe their food guides and key recommendations. Methods We conducted a systematic search to identify Spanish FBDGs targeted at the general population using multiple sources. Two authors independently screened the references, extracted data, and assessed the quality of the FBDGs using the Appraisal of Guidelines for Research and Evaluation (AGREE) II and the Recommendation Excellence (AGREE-REX) instruments. We performed a descriptive analysis of the FBDGs. Results We included 19 FBDGs, published between 2007 and 2019. The median scores for each AGREE II domain were: "scope and purpose" 44% (Q1-Q3: 33-61%); "Stakeholder involvement" 31% (11-44%), "rigor of development" 3% (1-14%); "clarity of presentation" 42% (33-47%), "applicability" 0% (0-6%); and "editorial independence" 0% (0-8%). Six FBDGs (32%; 6/19) were categorized as "recommended with modifications", and the rest (68%; 13/19) as "not recommended". None of the FBDGs scored >= 60% in three or more domains, including the "rigor of development" domain. FBDGs indexed in literature databases scored significantly higher in overall rating than those not indexed (P = 0.023). The majority of FBDGs (74%; 14/19) used the pyramid as a food guide representation with a larger number of food levels (3-7 levels). The majority of FBDGs recommended a daily intake of cereals and grains, vegetables, fruits, olive oil and dairy products; a weekly intake of vegetable and animal proteins; and the occasional and limited intake of other food groups (e.g., ultraprocessed foods). Conclusions Overall, the methodological quality of FBDGs is poor showing that only 32% of FBDGs are "recommended for use with modifications". Our results highlight the need to revise, systematize and improve FBDG development processes in Spain.

Polyglycerol esters (PGEs) are used as emulsifiers in recombined dairy cream (RDC) to improve product quality. In this study, the effects of four PGEs with different polymerization degrees and esterification on the particle size, viscosity, zeta potential, and microrheology of RDC emulsions were investigated, and the whipping time, overrun, serum loss, and firmness of the RDC emulsions were recorded. The results show that the addition of the PGEs reduced the particle size (from 2.75 μm to 1.48–1.73 μm) and increased the viscosity (from 41.92 cP to 73.50–100 cP) and stability (from 0.354 to 0.105–0.128), which were related to the change in interfacial properties and the weakening of Brownian motion, but there were differences in the effect on the whipping behavior of the RDCs. Although the addition of 0.9% triglyceride monolaurate gave the emulsion the best stability, the RDC had a longer whipping time (318 s) and a lower overrun (116.6%). Comparatively, the 0.7–0.9% concentrations of PGE55 and tripolycerol monostearate (TMS) provided RDC with good stability and aeration characteristics, allowing inflation within 100 s and expansion rates of up to 218.24% and 186.88%, respectively. In addition, the higher degree of polymerization of polyglyceryl-10 monstearate (PMS) did not work well at any concentration. These results contribute to understanding the mechanism of action of PGEs and improving the quality of RDC.

In this paper, we reviewed the role of dairy products in dietary zinc absorption. Dairy products can have a reasonable contribution for dietary zinc intake in Western diets, where dairy consumption is high. However, the co-ingestion of dairy products can also improve zinc absorption from other food products. Such improvements have been observed when dairy products (e.g., milk or yoghurt) were ingested together with food such as rice, tortillas or bread products, all of which are considered to be high-phytate foods with low inherent zinc absorption. For foods low in phytate, the co-ingestion of dairy products did not improve zinc absorption. Improved zinc absorption of zinc from high-phytate foods following co-ingestion with dairy products may be related to the beneficial effects of the citrate and phosphopeptides present in dairy products. Considering that the main dietary zinc sources in areas in the world where zinc deficiency is most prevalent are typically high in phytate, the inclusion of dairy products in meals may be a viable dietary strategy to improve zinc absorption.

In adulthood, the ability to digest lactose, the main sugar present in milk of mammals, is a phenotype (lactase persistence) observed in historically herder populations, mainly Northern Europeans, Eastern Africans, and Middle Eastern nomads. As the –13910∗T allele in the MCM6 gene is the most well-characterized allele responsible for the lactase persistence phenotype, the –13910C > T (rs4988235) polymorphism is commonly evaluated in lactase persistence studies. Lactase non-persistent adults may develop symptoms of lactose intolerance when consuming dairy products. In the Americas, there is no evidence of the consumption of these products until the arrival of Europeans. However, several American countries’ dietary guidelines recommend consuming dairy for adequate human nutrition and health promotion. Considering the extensive use of dairy and the complex ancestry of Pan-American admixed populations, we studied the distribution of –13910C > T lactase persistence genotypes and its flanking haplotypes of European origin in 7,428 individuals from several Pan-American admixed populations. We found that the –13910∗T allele frequency in Pan-American admixed populations is directly correlated with allele frequency of the European sources. Moreover, we did not observe any overrepresentation of European haplotypes in the –13910C > T flanking region, suggesting no selective pressure after admixture in the Americas. Finally, considering the dominant effect of the –13910∗T allele, our results indicate that Pan-American admixed populations are likely to have higher frequency of lactose intolerance, suggesting that general dietary guidelines deserve further evaluation across the continent.

The present study determined the consumption and preferences of dairy products, especially fermented products, in respect of Polish and Taiwanese students, through their answers to seven research questions (RQs) as part of the design process. The research for the study was conducted in two countries: in Poland at WULS and in Taiwan at NCHU. Two-hundred students in total (19–25 years old) were selected for the study. Responses to the research questions were collected using Paper & Pen Personal Interview (PAPI). The results indicate that Taiwanese students were less likely to declare consuming dairy products daily compared to Polish students (p < 0.0001). Poles declared consuming dairy products every day, or once or several times a week, while Taiwanese declared several times a week or several times a month. A total of 89% of Polish students indicated milk as their most commonly consumed dairy product, followed by yoghurts (80%) and ripened cheeses (69%). A total of 95% of Taiwanese students also mentioned milk as their most consumed dairy product, then yoghurt (especially drinking yoghurt) (81%), milk desserts (70%) and Yakult (69%). In the case of fat content preference in yoghurt, Poles significantly preferred yoghurts with regular fat content, while Taiwanese significantly preferred fatty yoghurts (p < 0.0001). The most common characteristic of yoghurt that influenced Polish students’ purchase decisions was its composition, followed by its consistency, appearance and its sour taste. However, for Taiwanese, the most important factors were the consistency, price and sweetness of the yoghurt. Polish students were significantly more willing to pay a higher price for a dairy functional product than Taiwanese students (p = 0.0009). The responses obtained show differences and similarities in the preferences and consumption of these products between Polish and Taiwanese students. The results of this study were used to direct the next stage of the project’s development research, the goal of which was to develop a functional dairy product that meets the preferences of consumers.

El estudio EsNuPI fue financiado por el Instituto Puleva de Nutrición (IPN). El patrocinador no tuvo ningún papel en el diseño del estudio, ni en la recopilación, el análisis e interpretación de los datos, la redacción del manuscrito, o la decisión de publicar los resultados Introducción: los hábitos de alimentación y los estilos de vida durante las primeras etapas de la infancia son importantes por su asociación a enfermedades crónicas durante la vida adulta. Objetivos: evaluar la ingesta de energía, el aporte de nutrientes, las principales fuentes alimentarias y los patrones dietéticos de los participantes en el estudio EsNuPI. Métodos: se utilizaron un cuestionario sociodemográfico, un cuestionario de frecuencia de consumo de alimentos, dos recuerdos de 24 h y un cuestionario de actividad física en dos cohortes de niños españoles de 1 a < 10 años, no veganos, residentes en municipios > 50.000 habitantes: una representativa de los consumidores de todo tipo de leches (SRS = 707) y otra consumidora de leches adaptadas (AMS = 741). Resultados: el 84,7 % de la cohorte SRS y el 83,5 % de la AMS mostraron una ingesta energética (IE) plausible y adecuada (1503 y 1404 kcal/ día, respectivamente) en ambas cohortes. El porcentaje de niños con ingesta proteica > 20 % de la IE fue del 12 % en la SRS y el 6 % en la AMS. Ambas cohortes sobrepasaron las recomendaciones de grasa total (36,5 % en la SRS; 35,9 % en la AMS) y de grasas saturadas (13,1 % vs. 12,1 %, respectivamente); la ingesta de DHA fue significativamente menor en la SRS (20 mg/d vs. 90 mg/d). La ingesta de vitamina D fue significativamente menor en la SRS, aunque ambas cohortes no alcanzaron las recomendaciones de los organismos internacionales. Al analizar los patrones alimentarios, destacan uno de alimentos apetitosos e hipercalóricos y dos de tipo mediterráneo. Conclusiones: es recomendable reducir la ingesta de grasas saturadas y proteínas, e incrementar la de calcio, vitamina D y magnesio, esto último especialmente en los niños > 4 años. Estos hallazgos son importantes para el diseño de intervenciones en la población infantil española. Introduction: eating habits and lifestyles during early childhood are important due to their association with chronic diseases in adulthood. Objectives: to evaluate energy and nutrient intake, main food sources, and dietary patterns in the EsNuPI study participants. Methods: a sociodemographic questionnaire, food frequency questionnaire, two 24 h recalls, and a physical activity questionnaire were used in two cohorts of Spanish children aged 1 to < 10 years, who were non-vegans living in municipalities > 50,000 inhabitants — one representative cohort of consumers of all types of milks (SRS = 707) and one other cohort consuming adapted milks (AMS = 741). Results: 84.7 % in SRS and 83.5 % in AMS showed a plausible, adequate energy (EI) (1503 and 1404 kcal/day, respectively). The percentage of children with protein intake > 20 % of EI was 12 % for SRS, and 6 % for AMS. Both cohorts exceeded the recommendations for total fat (36.5 % in SRS, 35.9 % in AMS) and saturated fat (13.1 % vs 12.1 %, respectively); DHA intake was significantly lower in SRS (20 mg vs 90 mg). Vitamin D intake was significantly lower in SRS, although both cohorts did not meet the recommendation. When analyzing dietary patterns, one of palatable energy-dense foods and two of Mediterranean type were highlighted. Conclusions: it is recommended that consumption of saturated fatty acids and protein be reduced, but calcium, vitamin D, and magnesium intakes should be increased, especially in children > 4 years. These findings are important for designing interventions in the Spanish child population. The findings of the EsNuPI study provide useful information for the design and promotion of appropriate interventions for Spanish children.

International audience; Despite a high level of sequence identity between cow's, goat's, and sheep's milk (CM, GM, and SM, respectively) proteins, some patients tolerant to CM are allergic to GM and SM. In most cases, this specificity is due to the presence of IgE antibodies that bind only to caprine and ovine caseins. The patients may then develop severe allergic reactions after ingestion of CM products contaminated with low amounts of GM or SM. We thus aimed to develop an assay able to detect traces of caprine/ovine β-caseins in different food matrices, irrespective of the presence of the bovine homolog. We produced monoclonal antibodies (mAbs) specific to caprine caseins in mice tolerized to the bovine whole casein then sensitized to the caprine whole casein. In order to develop a two-site immunometric assay, we selected mAbs that could discriminate the caprine β-casein from its bovine homolog. Characteristics and performances of two tests were determined with various dairy products. Results were analyzed in relation with the IgE-immunoreactivity of the food matrices, thanks to sera from CM, GM/SM allergic patients. Our two-site immunometric assays demonstrated a high sensitivity with a detection limit of 1.6–3.2 ng/mL of caprine and ovine β-caseins. The tests were able to detect contaminations of GM in CM at the ppm level. Heat-treatment, ripening and coagulation processes, usually applied to dairy products that exhibit a very high IgE-immunoreactivity, did not impair the test sensitivity. These quantitative assays could then be useful for the risk assessment of food products potentially contaminated with GM and SM in order to prevent adverse reactions in patients specifically allergic to these milks.

Background : The prevalence of gout increased based on age, highest at age 70-79 years 9.3%, age 60-69 years 8%, age 50-59 years 3.7%, and age 40-49 years 3.3%. Meanwhile, based on gender, the prevalence of gout diagnosed with health workers was higher in women at 13.4% compared to men 10.3% . Overweight and obesity can trigger an increase in uric acid levels, so it is one of a risk factor for hyperuricemia. Various high protein and purine foods have long been considered a risk factor for gout. Similarly, the possibility that consumption of dairy products especially cow's milk has a role in protecting the risk of gout based on the results of metabolic studies. Objective : To determine d the relationship of sex, body mass index (BMI) , dairy products consumption and uric acid levels of the elderly in Cipondoh Sub-District Tangerang. Method s : This study used a cross-sectional design. Research participants were collected by using purposive sampling. Primary data consisted of respondent characteristics, dairy products consumption, BMI, and uric acid levels. Results : The results of this research have shown there was a correlation between BMI and uric acid level p = 0. 00 7, dairy products consumption and uric acid level p = 0 . 0001, but there was no correlation between sex and uric acid level p = 0 . 204 in the elderly at Cipondoh Sub-District Tangerang. Conclusion : There was a correlation between BMI , dairy products consumption with uric acid levels of the elderly in Cipondoh Sub-District Tangerang.

The paper is focused on the issue of consumption of milk and dairy products as an important part of the rational nutrition of the population of the Slovak Republic. The aim of the paper is to highlight the development of consumption of drinking milk and selected dairy products, including cheese and acid-based products, in the last 20 years in the conditions of the Slovak Republic. Furthermore, the paper focuses on comparing current consumption of milk and dairy products with recommended intakes resulting from the rationalization of diet. Based on the results, it can be stated that the consumption of milk and dairy products is insufficient at the level of 70% of the recommended consumption intakes of the selected food group. In connection with this, it is important to note that the consumption of drinking milk is low. The results obtained by processing the secondary data were supplemented with the primary data obtained from the questionnaire survey. Based on the results, we have conclude that most consumers are trying to maintain a healthy lifestyle and rational diet, which is just the consumption of milk and dairy products. Consumers especially prefer drinking milk, cheese and yoghurt, whose consumption is still low, which the respondents attribute to the high prices of the monitored products as compared to their income. On the other hand, the results showed the main factors determining the consumption of milk and dairy products, among which we can include quality, composition, price, durability and nutrition data. Based on the results obtained by processing secondary data and primary research, we suggest informing and educating consumers about the positive health effects and highlighting the recommended benefits to a greater extent.

The food industry is looking for natural additives to improve acid curd cheese (tvarog), while shrimp by-products are being wasted. The concentrated astaxanthin lipid preparation (ALP) was recovered from shrimp shells and added (0%, 0.25%, 0.5% and 1%) to tvarogs stored up to 4 weeks at 5 ± 1 °C. The addition of ALP increased the lipid content and decreased the moisture in cheese. Water activity, acidity and hardness of tvarogs differed significantly between cheese variants. The cheeses with ALP had more stable and lower pH after 4 weeks of storage, and higher titratable acidity immediately after ALP addition. The 0–0.5% ALP samples had the same level and changes in lipid oxidation, while the 1% ALP cheese had more stable thiobarbituric acid values during storage. This may be due to several times greater antioxidant activity (DPPH assay) in the cheese with the highest ALP addition. The addition of astaxanthin had create popular salmon colour and improved objective colour parameters of the cheeses. The best sensory features had 0.5% ALP sample. A higher addition of astaxanthin preparation caused a foreign aftertaste. The use of astaxanthin from shrimp shells to acid curd cheeses enables the creation of new functional properties that are increasingly popular with consumers.

In dairy processing environments, many bacterial species adhere and form biofilms on surfaces and equipment, leading to foodborne illness and food spoilage. Among them, Listeria monocytogenes and Pseudomonas spp. could be present in mixed-species biofilms. This study aimed to evaluate the interactions between L. monocytogenes and P. fluorescens in biofilms simulating dairy processing conditions, as well as the capability of P. fluorescens in co-culture to produce the blue pigment in a Ricotta-based model system. The biofilm-forming capability of single- and mixed-cultures was evaluated on polystyrene (PS) and stainless steel (SS) surfaces at 12 °C for 168 h. The biofilm biomass was measured, the planktonic and sessile cells and the carbohydrates in biofilms were quantified. The biofilms were also observed through Confocal Laser Scanning Microscopy analysis. Results showed that only P. fluorescens was able to form biofilms on PS. Moreover, in dual-species biofilms at the end of the incubation time (168 h at 12 °C), a lower biomass compared to P. fluorescens mono-species was observed on PS. On SS, the biofilm cell population of L. monocytogenes was higher in the dual-species than in mono-species, particularly after 48 h. Carbohydrates quantity in the dual-species system was higher than in mono-species and was revealed also at 168 h. The production of blue pigment by P. fluorescens was revealed both in single- and co-culture after 72 h of incubation (12 °C). This work highlights the interactions between the two species, under the experimental conditions studied in the present research, which can influence biofilm formation (biomass and sessile cells) but not the capability of P. fluorescens to produce blue pigment.

In dairy processing environments, many bacterial species adhere and form biofilms on surfaces and equipment, leading to foodborne illness and food spoilage. Among them, Listeria monocytogenes and Pseudomonas spp. could be present in mixed-species biofilms. This study aimed to evaluate the interactions between L. monocytogenes and P. fluorescens in biofilms simulating dairy processing conditions, as well as the capability of P. fluorescens in co-culture to produce the blue pigment in a Ricotta-based model system. The biofilm-forming capability of single- and mixed-cultures was evaluated on polystyrene (PS) and stainless steel (SS) surfaces at 12 &deg, C for 168 h. The biofilm biomass was measured, the planktonic and sessile cells and the carbohydrates in biofilms were quantified. The biofilms were also observed through Confocal Laser Scanning Microscopy analysis. Results showed that only P. fluorescens was able to form biofilms on PS. Moreover, in dual-species biofilms at the end of the incubation time (168 h at 12 &deg, C), a lower biomass compared to P. fluorescens mono-species was observed on PS. On SS, the biofilm cell population of L. monocytogenes was higher in the dual-species than in mono-species, particularly after 48 h. Carbohydrates quantity in the dual-species system was higher than in mono-species and was revealed also at 168 h. The production of blue pigment by P. fluorescens was revealed both in single- and co-culture after 72 h of incubation (12 &deg, C). This work highlights the interactions between the two species, under the experimental conditions studied in the present research, which can influence biofilm formation (biomass and sessile cells) but not the capability of P. fluorescens to produce blue pigment.

Evidence has suggested that dairy product consumption lowers the risk of several cancers, but these benefits may not occur with bladder cancer. In a cohort of 101,721 subjects in the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cancer Screening Trial, we analyzed the effects of dairy product intake on bladder cancer risk using Cox proportional hazards regression. After a median of 12.5 years of follow-up, 776 new cases of bladder cancer were identified. We found no statistically significant association between total milk intake and bladder cancer risk. The multivariate-adjusted hazard ratio (HR) of bladder cancer for participants in the highest category of total milk intake compared with those in the lowest category was 1.13 (95% CI: 0.92–1.40; p for trend = 0.436). Among individual dairy foods, no statistically significant association was observed for a broad range of dairy products, including whole milk, 2% milk, 1% milk, skim milk, yogurt, regular butter, low fat butter, regular cheese, low fat cheese, and no fat cheese. These associations were not modified by smoking status (p for interaction > 0.05). In conclusion, findings from this large prospective analysis do not support an inverse association between dairy product consumption and bladder cancer risk.

Bacillus cereus is a common and ubiquitous foodborne pathogen with an increasing prevalence rate in dairy products in China. High and unmet demands for such products, particularly milk, raise the risk of B. cereus associated contamination. The presence of B. cereus and its virulence factors in dairy products may cause food poisoning and other illnesses. Thus, this review first summarizes the epidemiological characteristics and analytical assays of B. cereus from dairy products in China, providing insights into the implementation of intervention strategies. In addition, the recent achievements on the cytotoxicity and mechanisms of B. cereus are also presented to shed light on the therapeutic options for B. cereus associated infections.

Metabolic syndrome (MetS) is a prevalent disorder associated with diabetes and cardiovascular diseases. Lifestyle and occupation can increase the risk of developing MetS. Since dietary pattern is a major component of lifestyle, this study aimed to determine the relationship between consumption of animal source foods (ASFs) and MetS among food suppliers. This cross-sectional study was conducted on 112 male food suppliers. We measured anthropometric indices, body composition, and blood pressure of the participants. Blood biochemistry was determined using 5 mL fasting blood samples. MetS was defined based on the guidelines described by the International Diabetes Federation (IDF). ASF intake, including dairy products, eggs, red meat, poultry, and fish, was assessed using food frequency questionnaires. Overall, 46.4% of participants had MetS. Participants who consumed dairy 3∼5 times/d and more than 5 times/d had lower risk of MetS [odds ratios (OR): 0.18 (confidence interval (CI) 95%: 0.05∼0.62) and OR: 0.20 (CI 95%: 0.06∼0.67), respectively] compared with participants in the lowest tertile. The risk of hypertension was significantly decreased in participants who consumed dairy products >5 times/d [OR: 0.22 (CI 95%: 0.07∼0.67)]. Other ASFs were not associated against the risk of MetS in crude and adjusted models. Our findings indicated that adhering to dairy products can decrease the risk of MetS. Higher adherence to dairy products was also protective against hypertension in these participants.

The objective with this research was to compare milk quality parameters among herds characterized by different levels of milk production. The research involved 1080 bulk milk samples collected from 30 dairy herds, which produce milk for Parmigiano Reggiano cheese, during three years. Each milk sample was analysed for fat, crude protein, titratable acidity, total bacterial count, somatic cells, coliform bacteria, and Clostridia spores. Least mean values were obtained by ANOVA using milk production intervals from 6000 to 7999 (L-group), from 8000 to 9999 (M-group), and from 10000 to 12000 kg/cow/lactation (H-group) as fixed milk production classes. Increased milk production was associated with a reduction in milk fat content (from 3.54 in the L-group milk to 3.29 g/100 g in the H-group milk). An improvement in the hygienic-sanitary quality of milk was observed with increasing milk production. In fact, a progressive decrease in the total bacterial count (from 95 in the L-group milk to 45 103 cfu/mL in the H-group milk), number of coliforms (from 2294 in the L-group milk to 1342 cfu/mL in the H-group milk), and somatic cell number (from 382 in the L-group milk to 209 103 cells/mL in the H-group milk) was observed with the increase in milk production level. Finally, it appears that higher milk yield is connected with significant positive effects on the lactodynamometric properties of milk.

A novel and reliable method to quantify residual levels of N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine in dairy products using ion-pairing reversed-phase liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed and fully validated. Sample extraction was done with salting-out technique using acetonitrile and sodium chloride. For LC–MS/MS, the analyte was detected using positive electrospray ionization (ESI+) and two multiple reaction monitoring (MRM) transitions were monitored. The method was validated in the 5–150 µg kg−1 range using total error approach. Thus, performance criteria of the method were evaluated. Relative standard deviations for trueness and precision were lower than 10%; with the exception of hard pressed cheese at 5 µg kg−1 for precision. The limit of quantification (LOQ) was around 5–7 µg kg−1 depending on the matrix of interest. The method was successfully applied to accurately quantify N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine in 146 various dairy products with a maximum contamination level of 225 µg kg−1 in cheese.

Background Little is known about the interplay among dairy intake, gut microbiota and cardiometabolic health in human prospective cohort studies. Methods The present study included 1780 participants from the Guangzhou Nutrition and Health Study. We examined the prospective association between habitual dairy consumption (total dairy, milk, yogurt) and gut microbial composition using linear regression after adjusting for socio-demographic, lifestyle and dietary factors. The cross-sectional association of dairy-associated microbial features with cardiometabolic risk factors was examined with a linear regression model, adjusting for potential confounders. Serum metabolomic profiles were analyzed by partial correlation analysis. Findings There was a significant overall difference in gut microbial community structure (β-diversity) comparing the highest with the lowest category for each of total dairy, milk and yogurt (P < 0.05). We observed that dairy-associated microbes and α-diversity indices were inversely associated with blood triglycerides, while positively associated with high-density lipoprotein cholesterol. A follow-up metabolomics analysis revealed the association of targeted serum metabolites with dairy-microbial features and cardiometabolic traits. Specifically, 2-hydroxy-3-methylbutyric acid, 2-hydroxybutyric acid and L-alanine were inversely associated with dairy-microbial score, while positively associated with triglycerides (FDR-corrected P < 0.1). Interpretation Dairy consumption is associated with the gut microbial composition and a higher α-diversity, which provides new insights into the understanding of dairy-gut microbiota interactions and their relationship with cardiometabolic health. Funding This work was supported by the National Natural Science Foundation of China, Zhejiang Ten-thousand Talents Program, Westlake University and the 5010 Program for Clinical Researches of the Sun Yat-sen University.

Improving the nutritional and health properties of food products, e.g. infant milk formula, by the addition of functional ingredients is of primary importance. This study focused on bioactive milk polar lipids (PLs) recovered from dietary sources that are of increasing interest. The chemical compositions of buttermilks and butter serums were determined and the modulation of the fatty acid composition of milk PLs was investigated. Butter serums contain a higher amount of milk PLs than buttermilks (88 vs. 13-18g/kg dry matter), with a higher proportion of sphingomyelin (34 vs. 19% of PLs, respectively) interestingly close to human milk PL profile. Butter serums are also interesting sources of choline, an important nutrient for infant brain development. We demonstrated that the unsaturated fatty acid content of milk PLs recovered in the buttermilks and the butter serums, mainly the amount of C18:3n-3 and C22:6n-3 (DHA) that are of nutritional interest, can be increased by dietary strategies. This work opens perspectives for a better valorization of milk PLs in human nutrition (both infants and adults) to benefit their functional, nutritional and health properties.

In this research two-dimensional GC was used to analyse, for the first time, the detailed fatty acid (FA) profiles of 11 dairy matrices: raw milk (evening whole, evening partially skimmed, morning whole, and vat milk), cream, fresh cheese, whey, ricotta, scotta, 6- and 12-month-ripened cheeses, obtained across artisanal cheese- and ricotta-making trials carried out during the summer period while cows were on highland pastures. Samples were collected during 7 cheese- and ricotta-making procedures carried out at 2-week intervals from bulk milk to study possible differences in the transfer and modification of FA. Compared with morning milk, evening milk had fewer de novo synthetised FA. The detailed FA profile of partially skimmed milk differed little from that of evening whole milk before skimming, but the cream obtained differed from partially skimmed milk and from fresh cheese in about half the FA, due mainly to higher contents of all de novo FA, and lower contents of n-3 and n-6 FA. Fresh cheese and whey had similar FA profiles. The ricotta manufacturing process affected the partition of FA between ricotta and scotta, the FA profile of the latter differing in terms of groups and individual FA from the former, whereas ricotta and fresh cheese had similar composition of FA. In general, there was an increase in medium-chain saturated FA, and a decrease in many polyunsaturated FA during the first 6 months of ripening, but not during the second 6 months. Two-dimensional GC yielded a very detailed and informative FA profile on all the 11 dairy products and by-products analysed.

Understanding the behavior and structure of food products requires knowledge of the spatial arrangement of the various components and their interactions. Among these components, proteins, beyond their roles in nutrition and health, exhibit specific functional properties inherited from both their molecular flexibility and the plasticity of their diversified assemblies. These structural and functional properties make food proteins versatile and useful ingredients in a wide range of product development. This chapter reviews the role of proteins in the development of food structure with a focus on milk and egg proteins. We first describe the main proteins, their physicochemical characteristics, and the diversity of assembled structures obtained during processing. Through specific examples, including proteins gels, yogurt, and various cheeses, we illustrate how the properties of proteins are explored in the manufacture of food.

The use of probiotic bacteria derived from fermented foods has been explored by the scientific community as alternative strategies for the treatment of several diseases, mainly regarding intestinal dysfunction. One of the most relevant inflammatory diseases affecting the alimentary tract, for which no current intervention is entirely successful, is mucositis. In this review article, we summarize the most recent proof-of-concept studies dealing with the therapeutic use of dairy origin probiotics, for the treatment of gastrointestinal mucositis. Furthermore, we discuss several approaches for the improvement of the classical therapeutic rationale, such as supplementation with prebiotics and genetic engineering along with the respective translational issues, which may be crucial for the successful transposition of these therapeutic strategies for clinical use.

Aim: To characterize four novel autochthonous bifidobacteria isolated from monkey faeces and a Bifidobacterium lactis strain isolated from chicken faeces by evaluating their technological and biological/functional potential to be used as probiotics. Different stressors, including food process parameters and storage, can affect their viability and functionality. Methods and Results: The resistance to frozen storage, tolerance to lyophilization and viability during storage, thermal, acidic and simulated gastric resistance, surface hydrophobicity and antimicrobial activity against pathogens were studied. Bifidobacterium lactis Bb12 and INL1 were used as reference strains. The results obtained demonstrated that the new isolates presented strain-dependent behaviour. Good results were obtained for thermal resistance, frozen storage at −80°C and lyophilized powders maintained at 5°C. Cell viability during refrigerated storage was higher when the strains were resuspended in milk at pH 5·0 than at 4·5. The surface hydrophobicity ranged between 7 and 98% depending on the strain. The simulated gastric resistance was improved for the strains incorporated in cheese. Regarding antimicrobial activity, bifidobacteria isolated from monkey presented higher inhibitory capacity than the reference strains. Conclusion: This research provides a deeper insight into new strains of bifidobacteria isolated from primates and chicken that have not been previously characterized for their potential use in dairy products and confirm the most robust stress tolerance of B. lactis. Significance and Impact of the Study: The possibility of expanding the available bifidobacteria with the potential to be added to a probiotic food necessarily implies characterizing them from different points of view, especially when considering unknown species. For monkey isolates (which showed higher antimicrobial activity against pathogens), more in-depth knowledge is needed before applying strategies to improve their performance. On the contrary, the chicken isolate B. lactis P32/1 showed similar behaviour to the references B. lactis strains; therefore, it could be considered as a potential probiotic candidate. Fil: Sarquis, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Siroli, Lorenzo. Dipartimento Di Scienze E Tecnologie Agro-alimentari; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Modesto, Mónica. Universidad de Bologna; Italia Fil: Patrignani, Francesca. Dipartimento Di Scienze E Tecnologie Agro-alimentari; Italia Fil: Lanciotti, Rosalba. Dipartimento Di Scienze E Tecnologie Agro-alimentari; Italia Fil: Mattarelli, Paola. Universidad de Bologna; Italia Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Burns, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina

The aim of the present study was to determine if space allocation influenced the concentration of biomolecules in buffalo milk and dairy products. Intensively housed buffaloes (n = 96) were randomly assigned to 2 groups according to days in milk, parity, and milk yield: group S10 had a space allocation of 10 m2 per buffalo and group S15 had a space allocation of 15 m2 per buffalo. Individual milk yield was recorded daily. Twice a month, a bulk milk sample was collected for each group, as well as whey, ricotta, and mozzarella cheese, to assess cheese yield and to conduct HPLC-electrospray ionization-tandem mass spectrometry, milk antioxidant activity, and cell viability analyses. We tested milk extracts from the 2 groups in vitro to evaluate their efficacy in counteracting endothelial oxidative damage induced by high glucose. We evaluated reproductive function in 28 buffaloes from each group using the Ovsynch-timed artificial insemination program. We observed no differences in milk quantity or quality in terms of fat, protein, or lactose, and reproductive function did not differ between the 2 groups. Compared with group S10, group S15 had higher concentrations of carnitine (56.7 ± 1.1 vs. 39.8 ± 0.7 mg/L in milk and 40.9 ± 0.8 vs. 31.7 ± 0.7 mg/L in whey), acetyl- l -carnitine (51.9 ± 0.3 vs. 39.7 ± 0.7 mg/L in milk and 41.1 ± 1.7 vs. 28.7 ± 2.6 mg/L in whey), propionyl- l -carnitine (34.8 ± 1.0 vs. 21.0 ± 0.9 mg/L in milk and 26.9 ± 0.8 vs. 17.6 ± 1.2 mg/L in whey), glycine betaine (23.1 ± 1.9 vs. 13.5 ± 1.6 mg/L in milk and 10.7 ± 0.4 vs. 7.9 ± 0.5 mg/L in whey), and δ-valerobetaine (24.2 ± 0.5 vs. 16.7 ± 0.5 mg/L in milk and 22.0 ± 0.9 vs. 15.5 ± 0.7 mg/L in whey). Group S15 also had higher total antioxidant activity than group S10 (56.7 ± 1.9 vs. 46.4 ± 1.13 mM Trolox equivalents). Co-incubation of high-glucose-treated endothelial cells with milk extracts from group S15 improved cell viability compared with cells treated with high glucose only; it also reduced intracellular lipid peroxidation (144.3 ± 0.4 vs. 177.5 ± 1.9%), reactive oxygen species (141.3 ± 0.9 vs. 189.3 ± 4.7 optical density units), and cytokine release (tumor necrosis factor-α, IL-1β, IL-6). Greater space allocation was associated with higher levels of biomolecules in buffalo milk. This could have been the result of improved welfare in buffaloes that were allocated more space.

Evidence on consumption of dairy foods and human health is contradictory. This study aimed to summarize the level of evidence of dairy consumption on various health outcomes. A systematic search for meta-analyses was performed: study design, dose–response relationship, heterogeneity and agreement of results over time, and identification of potential confounding factors were considered to assess the level of evidence. Convincing and probable evidence of decreased risk of colorectal cancer, hypertension and cardiovascular disease, elevated blood pressure and fatal stroke, respectively, was found for total dairy consumption; possible decreased risk of breast cancer, metabolic syndrome, stroke and type-2 diabetes, and increased risk of prostate cancer and Parkinson’s disease was also found. Similar, yet not entirely consistent evidence for individual dairy products was reported. Among potential confounding factors, geographical localisation and fat content of dairy have been detected. In conclusions, dairy may be part of a healthy diet; however, additional studies exploring confounding factors are needed to ascertain the potential detrimental effects.

Dry heating (DH) at 100 °C for 36 h of a whey protein isolate powder conditioned at pH 9.5 leads to the formation of stable, large and porous whey protein microparticles (PMs), resulting from the crosslinking of proteins inside the powder. These PMs could be used as high-viscosity food ingredients. Casein, present as a contaminant in whey protein powders, has been shown to become incorporated into the PMs. In this study, we investigated the effect of adding increasing amounts of sodium caseinate to whey protein powders on the formation of PMs during DH at 100 °C for 36 h. In addition, we studied PM formation during DH of a micellar casein-enriched milk protein powder (Casmic). The browning index of the dry-heated powders, and the size and water content of the microparticles were also characterized. We confirmed that sodium caseinate was incorporated into the PMs. The highest PM D[4,3] values (270 μm) were observed for powders with around 40% caseinate. Powders without added caseinate displayed D[4,3] values of 150 μm. The yield of conversion of proteins into PMs increased from 0.6 to 0.8 g/g with caseinate addition, whereas the amount of water entrapped in the PMs decreased from around 30 to 20 g/g. PMs were also formed by DH of the Casmic powder, but these particles were smaller, with sizes of around 80 μm. In conclusion, our study shows that the process of DH at pH 9.5 could be applied to all milk proteins to obtain PMs with functional properties that could be used in the food industry.

Modulation of the microbiota of patients suffering from inflammatory bowel disease (IBD) with probiotics was suggested to avoid active disease and/or to lower the intensity of the active phase. Considering that the ecological niche of Bifidobacterium spp and Lactobacillus spp includes fermented dairy products, and that countries with highest dairy product consumption were those with lowest prevalence of IBD, we aimed at determining if consumption of dairy products could modulate the prevalence of IBD. Linear regression was performed on data from the Global Burden of Diseases, Injuries, and Risk Factors Study reporting the prevalence of IBD and from the European Association of Dairy Trade reporting national consumptions of dairy products. Consumption of milk, cheese or yoghourt per capita did not correlate with the prevalence of IBD at the national level in Western countries. Considering the limitations of this type of analysis, we recommend to continue investigating the effect of dairy product consumption on IBD by cross-sectional, longitudinal or interventional studies.

Perishable products require special handling measures that may have social and environmental impacts along with their well-known economic aspects. Therefore, the sustainability of food supply chains has gained ground; however, the sustainability of perishable food supply chains, is still not a fully explored field of study. Therefore, in this research, a multi-objective mathematical programming model is developed to optimize the cost, energy consumption, and the traffic congestion associated with such supply chain operations. In this study, product lifetime uncertainty is explicitly modeled as a Weibull random variable, and food perishability is assumed to be affected by vehicle refrigerator utilization, which is considered as a decision variable. In addition, multiple vehicle types and multiple product types are considered. A dairy supply chain case is investigated, and the interrelations and interactions of all three aspects of sustainability, also known as the three triple bottom lines (TBL) of sustainability, are studied. The results indicate that emphasizing the economic aspect, for highly perishable products, the environmental impact of the chain may increase by 120%, and for the highly congested road networks, the social impact may rise by 51%. However, a 15% economic compromise can improve the sustainability of the supply chain network design by 150%. It is also shown that road congestion and the uncertain perishability of the products are critical factors that can, although differently, affect the operation and the design of the supply chain. This study contributes to the sustainable development goals (SDG’s) such as Zero Hunger (SDG 2); Affordable and Clean Energy (SDG 7); Decent Work and Economic Growth (SDG 8); Industry, Innovation, and Infrastructure (SDG 9); Responsible Consumption and Production (SDG 12); Climate Action (SDG 13) and Peace, Justice, and Strong Institutions (SDG 16). The results suggest that decision-makers can significantly reduce the environmental and social influences of the supply chain even without drastically compromising the economic aspect.

Several studies have assessed the effects of milk and dairy product intake on sleep quality and duration. Such investigations have varied in terms of their geographic locations, amounts of milk and dairy products, study participants (age, sex, race), and study designs. The present study aimed to summarize this literature and provide a unified view on whether the intake of milk and dairy products affects sleep quality. This systematic review was conducted according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. The following keywords were chosen as electronic database search items from MeSH (medical subject headings) terms and descriptors in health sciences (DeHS) lists: milk, yogurt, dairy product, cheese, sleep, human, observational study, and interventional study. As a result, a total of 14 studies published between 1972 and 2019 were included in this review, including eight randomized controlled trials, two experimental studies with cross-over designs, one longitudinal study, and three cross-sectional studies. Four studies targeted older adults, three included toddlers, two targeted children, and six enrolled adults inclusive of university students. Overall, these studies indicated that a well-balanced diet that includes milk and dairy products is effective in improving sleep quality, despite mixed results across studies attributable to differences in study populations and methods.

Background and Purpose :Aflatoxin M1, in milk and dairy products, is classified by the International Agency for Research on Cancer as human carcinogens (class 2B). The aim of this study was to evaluate aflatoxin M1 contamination level in dairy products from Iranshahr city, Iran, using ELISA technique. Materials and Methods: In our study, 87 samples of milk, white cheese, yoghurt, and butter were collected in different seasons and after preparation, were analyzed by ELISA technique. Results: The contamination level of aflatoxin M1 in pasteurized milk (n=40) yoghurt (n=15), white cheese (n=6), and butter (n=10) samples were determined to be 81.6%, 83.3%, 60%, and 100%, respectively. However, in 8.1% of the pasteurized milk samples, aflatoxin M1 concentration was above the legal accepted limit (>50 ng). The level of aflatoxin M1 was significantly higher in winter compared to summer (P

The possibility to apply UV-C light technology to control surface contamination and extend the shelf-life of Fiordilatte was investigated. First, cheese was inoculated with Pseudomonas spp., exposed to UV-C light for increasing time up to 750 s to estimate the antimicrobial efficacy of the treatments. UV-C light penetration depth in Fiordilatte was also evaluated. Then, a shelf-life test was carried out on samples exposed to 0.1, 0.6, 1.2 and 6.0 kJ/m 2 UV-C light, compared to untreated control cheese. The samples were packaged with brine, stored at 9 °C and analyzed for microbial growth, sensory quality and pH. A germicidal effect of about 1–2 log cycles on Pseudomonas spp. and Enterobacteriaceae was observed during storage. UV-C light did not promote changes in terms of color, texture and surface appearance. With a minimum transmittance inside the product, this treatment showed an interesting surface microbial decontamination that prolonged cheese shelf-life. Industrial relevance Considering that dairy industry represents one of the most important components of the Italian food system, the present work focused on the utilization of UV-C light to preserve one of the most important milk-derived Italian fresh cheese, Fiordilatte, which totalized a consumption of about 20 kg per capita per year in the world. Interesting results were recorded on treated samples, above all at specific UV-C light fluence values (6 kJ/m 2 ). The control of microbial proliferation in these treated samples allowed prolonging shelf-life by 80% compared to untreated cheese. The technique is very rapid and simple to be scaled up; after proper optimization of light parameters, it could be applied at industrial level to prevent surface post-process contamination of Fiordilatte that generally represents the main factor responsible for product deterioration and its short shelf-life.

Bacillus cereus is a common and ubiquitous foodborne pathogen with an increasing prevalence rate in dairy products in China. High and unmet demands for such products, particularly milk, raise the risk of B. cereus associated contamination. The presence of B. cereus and its virulence factors in dairy products may cause food poisoning and other illnesses. Thus, this review first summarizes the epidemiological characteristics and analytical assays of B. cereus from dairy products in China, providing insights into the implementation of intervention strategies. In addition, the recent achievements on the cytotoxicity and mechanisms of B. cereus are also presented to shed light on the therapeutic options for B. cereus associated infections.

The objective of this study was to evaluate milk quality for somatic cell count (SCC) analysis and total bacterial count (TBC) in agroecological production systems and conventional systems, relating them to the frequency of milking practices and types of facilities. The study was conducted in 2013 and 2014, in the western region of Paraná State, Brazil. We used a semi-structured questionnaire to identify management practices of producers in the 17 dairy systems (DS) in the city of Diamante d'Oeste and the 27 DS in Toledo. Milk sampling was performed monthly. Data were analyzed using two statistical methods. These were then used to create cross tabulation of groups with production systems and practices that can influence product quality. Cluster classification analysis allowed grouping of the initial 44 production systems into three homogeneous groups: Group 1, with better quality regarding the normative instruction 62 (NI62), with average values of SCC 407.37 (cells × 1000/mL) and TBC 210.50 (cfu × 1000/mL); Group 2, with SCC 328.50 (cells × 1000/mL) and TBC 699.75 (cfu × 1000/mL), both outside the NI62 parameters; and Group 3, with SCC 680.25 (cells × 1000/mL), considered low- and high-value TBC 1775.25 (cfu × 1000/mL), out of NI62. The results indicate that management practices and some other preventions adopted during milking are the determining factors of milk quality. Therefore, SCC and TBC levels are not determined by agroecological or conventional production systems, but rather, these levels are determined by the practices differentially adopted by the production systems.

There is a physiological basis for the roles of selected nutrients, especially proteins, calcium, and vitamin D, in growth and development, which are at a maximum during the pediatric period. Milk and dairy products are particularly rich in this group of nutrients. The present systematic review summarizes the available evidence relating dairy product intake with linear growth and bone mineral content in childhood and adolescence. A search was conducted in the MEDLINE (via PubMed) and SCOPUS databases following Preferred Reporting Items for Systematic Reviews and Meta- Analyses (PRISMA) guidelines and included intervention-controlled clinical trials with dairy products in children from 1 January, 1926 to 30 June, 2018. The risk of bias for each study was assessed using the Cochrane methodology. The number of study participants, the type of study and doses, the major outcomes, and the key results of the 13 articles included in the review are reported. The present systematic review shows that supplementing the usual diet with dairy products significantly increases bone mineral content during childhood. However, the results regarding a possible relation between dairy product consumption and linear growth are inconclusive., This study was partially funded by the University of Granada Plan Propio de Investigación 2016, Excellence actions: Unit of Excellence on Exercise and Health (UCEES), Plan Propio de Investigación 2018, Programa Contratos-Puente, the Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades, and European Regional Development Funds (ref. SOMM17/6107/UGR).

Brucellosis is among the most prevalent zoonoses caused by Brucella, transmitted to humans through the consumption of milk and dairy products. Currently, the Rev1 (sheep) and IRIBA (cow) vaccines are in use to protect the livestock from Brucella in Iran, while the diagnostic methods include the culturing and serologic methods. In this regard, the molecular polymerase chain reaction (PCR) diagnostic methods are quicker, more precise, and more sensitive than cultures, offering higher specificity than serology in the diagnosis of brucellosis. This research was an attempt at the PCR assay of the contamination of the milk from the livestock in Jahrom County, which had been vaccinated against the Brucella bacterium with the IRIBA and Rev1 vaccines. This research was a cross-sectional descriptive study carried out on 941 milk samples which were collected using the cluster random sampling technique. The Bioneer PCR Premix, Korea, was used to carry out the PCR. The overall contamination of the milk from the livestock vaccinated against the Brucella bacterium was 19%. The contamination was also 18% and 20% in the livestock vaccinated with the Rev1 and IRIBA vaccines, respectively. The findings from this research are reflective of the presence of the Brucella species in the milk samples of the vaccinated livestock.

Alongside the rapid growth in aging populations, prevention of age-related memory decline and dementia has become a high priority. Several epidemiological and clinical studies have concluded that fermented dairy products can help to prevent cognitive decline; furthermore, intake of Camembert cheese prevents Alzheimer's pathology in model mice. To elucidate molecular mechanisms underlying the preventive effects of fermented dairy products, here we screened peptides from digested fermented dairy products for ability to improve memory function in a scopolamine-induced amnesia mouse model. We found that Trp-Tyr (WY)-containing peptides from whey protein improved memory function in the mice, and the effects were confirmed in aged mice. The WY-containing peptides directly inhibited monoamine oxidase-B activity and increased dopamine levels in brain tissue. Pretreatment with dopamine receptor antagonist abolished the improvement in memory function due to WY-containing peptides. These results suggest that WY-containing peptides in fermented dairy products increase monoamine levels by inhibiting monoamine oxidase-B activity, helping to prevent age-related cognitive decline.

In this study, the presence of genes responsible for the pathogenicity and antibiotic resistance profile of enterococci isolated from various foodstuffs of animal origin was investigated. The percentage prevalence of enterococci was 54.1% (203/375) and the average count was found to be 3.81 log cfu/ml-g. Species-specific primers revealed Enterococcus faecalis as the predominant species carrying one or more virulence-associated traits of efa, gelE, ace, esp and agg genetic markers. Only one E. faecium isolate (from milk) was positive for the esp gene. Regarding antibiotic resistance, the highest frequency of resistance was observed for tetracycline (21.7%), followed by quinupristin/dalfopristin (13.3%), ciprofloxacin (2.0%), penicillin (2.0%), linezolid (1.0%), ampicillin (1.0%), streptomycin (1.0%), and gentamicin (0.5%). Enterococcus faecalis showed a higher prevalence of antibiotic resistance than other enterococci. The percentage of multidrug resistance among the isolates was 3.4%. Twenty-nine E. faecalis isolates (26.6%) carrying one of the virulence-associated traits were at the same time resistant to at least one antibiotic. Our results show that foods of animal origin, including ready-to-eat products, may be reservoirs of antibiotic-resistant and potentially virulent enterococci.