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The effect of planting time (September, October and November) and the size of flower bulbs (12.1-14, 10.1-12 and 8.1-10 cm) on flower quality and flowering period of natural Narcissus tazetta naturally grown in Ordu in Black Sea region were investigated. Tepal width, tepal length, corona diameter, corona length, perianth tube length, flower size, flower stem length, flower stem diameter, the pedicel length, ovary length, the number of flowers per stem, flower diameter, number of leaves, leaf width, leaf length, vase life, yield and flowering period were determined. The best quality of flowers as well as the highest flowering rate was obtained from the flower bulbs with a circumference of 12-14 cm. The smaller bulbs with a circumference of 10-12 cm gave only a few flowers whereas the smallest ones did not give any flowers. Therefore, it is suggested that the minimum circumference of the bulbs should be at least 12-14 cm for cut flower production of Narcissus tazetta. September was the best planting time for Narcissus tazetta cut flower production as to the flower quality and harvest period. The flowering period was extended to about 3 months from January 15 to April 11 by planting the bulbs at 3 different times. © 2019 International Society for Horticultural Science. All rights reserved. This research project (PYO.ZRT. ? ? ? ?. ? ?. ? ? ?) was supported by Project Management Office of Ondokuz Mayis University in Samsun, Turkey.

Lilium longiflorum is a wild Lilium, and its flowering transition requires a long period of cold exposure to meet the demand of vernalization. The responses of different sized bulbs to cold exposure and photoperiod are different, and the floral transition pathways of small and large bulbs are different. In this study, small and large bulbs were placed in cold storage for different weeks and then cultured at a constant ambient temperature of 25 °C under long day (LD) and short day (SD) conditions. Then, the flowering characteristics and expression patterns of key genes related to the vernalization and photoperiod pathways in different groups were calculated and analyzed. The results showed that the floral transition of Lilium longiflorum was influenced by both vernalization and photoperiod, that vernalization and LD conditions can significantly improve the flowering rate of Lilium longiflorum, and that the time from planting to visible flowering buds’ appearance was decreased. The flowering time and rate of large bulbs were greatly influenced by cold exposure, and the vernalization pathway acted more actively at the floral transition stage. The floral transition of small bulbs was affected more by the photoperiod pathway. Moreover, it was speculated that cold exposure may promote greater sensitivity of the small bulbs to LD conditions. In addition, the expression of LlVRN1, LlFKF1, LlGI, LlCO5, LlCO7, LlCO16, LlFT1, LlFT3 and LlSOC1 was high during the process of floral transition, and LlCO13, LlCO14 and LlCO15 were highly expressed in the vegetative stage. The expression of LlCO13 and LlCO14 was different under different lighting conditions, and the flowering induction function of LlCO9 and LlFT3 was related to vernalization. Moreover, LlFKF1, LlGI, LlCO5, LlCO16, LlSOC1 and LlFT2 were involved in the entire growth process of plants, while LlCO6, LlCO16 and LlFT1 are involved in the differentiation and formation of small bulblets of plants after the inflorescence stage, and this process is also closely related to LD conditions. This study has great significance for understanding the molecular mechanisms of the vernalization and photoperiod flowering pathways of Lilium longiflorum.

Khon Kaen Agriculture Journal, 49, 6, 1563-15571

Constraints are reported for the low productivity and quality of onion seed production in Ethiopia. Therefore, a field experiment was conducted at Kobo sub-center research station and Libso investment farm to study the influence of planting date and onion bulb size on yield and quality of onion seed. The experiment was laid out in Randomized Complete Block Design with three replications and involved three levels of onion bulb sizes and four levels of planting dates. The results of the study revealed that significant interactions between planting date and mother bulb size on seed yield ton ha-1, germination percentage, seed vigor index I and seed vigor index II. The highest seed yield (2.8 tons ha−1) was recorded from large onion bulbs of a size planted at Kobo early—October. Planted onion bulbs early-October increased seed yield (ton ha−1 by 21.7%) than planted early-November. Regarding onion bulb size, large bulbs increased seed yield (ton ha−1 by 40%) than the small bulbs. The highest (97.3) germination percentage was recorded from large onion bulbs planted at Kobo in early-October. Therefore, for the production of better yield and quality of onion seeds, early-October planting and large onion bulbs (4.1–5 cm) can be recommended in the study areas and other similar agro-ecology areas of the country.

The combined application of humic substances and mycorrhizal inocula under increased CO in the air is a promising horticultural technique for improving the quality of greenhouse-grown onion seedlings. The objective of this study was to evaluate if the development and qualitative characteristics of bulbs from the yellow onion Allium cepa L. Alfa São Francisco improved with the application of the abovementioned agronomic, biotic, and environmental factors. The lowest dry matter was achieved in bulbs of onions not inoculated with mycorrhizal fungi, non-amended with humic acids (HA), and grown at ambient CO. Mycorrhizal inoculation significantly increased bulb biomass in plants cultivated at ambient CO, and not fertilized with HA, which may be a consequence of the enhanced acid phosphatase activity in roots. The application of HA always increased bulb dry matter, which were more evident at elevated rather than ambient CO conditions and correlated with improved acid phosphatase activity in the plants at elevated CO. Mycorrhizal inoculation, HA supplementation, and elevated CO in the air had an additive effect of increasing the content of soluble sugars, proteins, and phenolics in bulbs. While high levels of sugars and proteins enhanced the energetic value of onions, the increase in phenolics improved their antioxidant properties. The triple interaction between mycorrhization, HA fertilization, and elevated CO also increased the ratio between soluble solids and total titratable acidity, which may enhance the perception of sweetness and make onions more pleasant for consumption., This study has been supported by the ‘Ministerio de Ciencia e Innovación’ (Spain) (BFU2011-26989). Marcelle M. Bettoni received a grant from ‘Los CAPES y Coordenação do Programa de Pós-graduação em Agronomia - Produção Vegetal’ from the Brazilian Government.

Two field experiments were carried out. In the first experiment, 13 variables were studied for 3 inbred groups as influenced by genetic differences, source of pollen, soil moisture, fertilizers , and size of bulbs. Path-coefficient analysis was made of var ious insect pollinators influencing seed yield in different inbreds, and of components of seed production in all inbreds together. In the second experiment, 4 variables were studied for one male-sterile inbred and one male-fertile inbred as influenced by soil moisture and fertilizers. Genetic differences among inbreds were the most important source of variation in seed yields. This points out that a major portion of the onion seed problem could be solved through plant breeding. The inbreds differed very significantly in t heir attractiveness to pollinating insects, probably as a result of varying genetic vigor which influenced nectar formation. Most of the inbreds had negative correlation or no correlation between the number of honey bee nectar collectors and seed yield. The separation of correlation with honey bee nectar and pollen collectors into components of direct and indirect effects indicates that the number of pollinating insects was excessive for all inbreds in the experiment. Negative direct effects on seed yield and negative indirect effects through percent fertilized flowers and seeds per fertilized flower disclose some important aspects as possible causes for low onion seed yields: 1. Depletion of assimilated food needed for the development of fertilized ovules through an overdraw on nectar by nectar collectors. 2. Water deficit affecting fertilized developing ovules due to increased transpiration resulting from overhandling of flowers by honey bees, especially by pollen collectors, which through nervous search for pollen on male- sterile lines might cause enough physical damage to increase transpiration even at low visitation levels. At high visitation levels, pollen collectors might also harm male-fertile lines. 3 . Removal of pollen from the stigmas of male-sterile flowers by high populations of pollen collecting honey bees. Compatibility of the pollen parents with the male-sterile inbreds was influenced by soil moisture levels. At high moisture, M611C increased seed yields while B12115-2C decreased them. At low moisture the reverse was true. In the first experiment there was no significant yield response to varying soil moisture levels due to contrasting effects of high soil moisture on compatibility with pollen parents. In the second experiment, the pollen parent B2215C was used and a significant favorable response to high soil moisture was obtained. Salinity aspects and the effects of indiscriminate use of fertilizers were tested through the method of fertilizer placement. While fertilizers reduced seed yield , high soil moisture tended to reduce the negative effects of nitrogen and to produce favorable response to phosphorus. Seed yield followed the trend in plant survival as affected by the induced salinity x moisture relationships. The size of bulbs had a great influence on seed yields, mainly through an increased number of umbels per plant. It seems that the number of umbels per plant had more influence on the number of flowers per row than did the number of flowers per umbel. Path-coefficient analysis of components of onion seed production indicates that the percentage of fertilized flowers is the most important criterion for selection to improve seed yield. It would be ideal to combine both high percentage of fertilized flowers and large number of flowers per umbel, but if compromise has to be made, selection would best be considered on the basis of greater percentage of fertilized flowers in the umbel. Plants which have a large percentage of fertilized flowers can be easily detected at the pre-seed harvest stage.