1. Physiological and Molecular Characterization of Cephaleuros virescens Occurring in Mango Trees
- Author
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Thiago Alves Santos de Oliveira, Daniel Diego Costa Carvalho, Fabíola Teodoro Pereira, Nei Peixoto, Camila Vilela Vasconcelos, and Elizabeth Amélia Alves Duarte
- Subjects
0301 basic medicine ,Trebouxia ,biology ,Petri dish ,Mangifera indica ,macromolecular substances ,algal spot disease ,lcsh:Plant culture ,biology.organism_classification ,Isolation (microbiology) ,law.invention ,Agar plate ,03 medical and health sciences ,030104 developmental biology ,Algae ,law ,algal isolation ,Botany ,lcsh:SB1-1110 ,Agronomy and Crop Science ,Cephaleuros virescens ,Pathogen ,Mycelium - Abstract
The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.
- Published
- 2018
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