Search

Based on the potassium channel "origami windmill" model, and the conservation law of cell membrane area and ion inequality equation of based on the potassium channel "origami windmill" model, and Maxwell's electromagnetic theory, it is theoretically proved that neurons can generate electromagnetic waves. The electromagnetic wave is an energy wave, never disappear. Neurons are equivalent to engineering antennas, and information between neurons can be transmitted through electromagnetic waves. The material basis for neurons to generate electromagnetic waves is the result of the exchange of cations on the inner surface of the cell membrane, especially Na+ and K+; The essence of consciousness should be electromagnetic wave. The conclusion that "neurons can generate electromagnetic waves" provides theoretical support for human beings to finally solve the mystery of the brain. At the same time, the author gives seven falsification schemes. The brain is a huge gold mine, and it is too important to crack the mystery of the brain. It should be a joint operation of "multiple arms". It should not only be the work of brain scientists, but also the participation of physicists, chemists and mathematicians.

The author made corrigendum to the article Conservation Law of Cell Bioelectricity Membrane Area and Ion Inequality Equation Based on Potassium Channel "Origami Windmill" Model of two clerical errors:Original content:The third paragraph of "1.2 Physical phenomena of cell membrane ion exchange", "Action potential, relative to K+, rises from −40mV to +40mV, which is the process of intracellular K+ from the L channel uniform deceleration flowing to the extracellular;" "the decrease from +40mV to −60mV, is a process of uniform deceleration of intracellular Na+ from the L channel to the extracellular."The "uniform deceleration" mentioned twice is changed to "uniform acceleration".Content after change:"Action potential, relative to K+, rises from −40mV to +40mV, which is the process of intracellular the amount of K+ from the L channel uniform acceleration flowing to the extracellular;" "the decrease from +40mV to −60mV, is a process of uniform acceleration of intracellular the amount ofNa+ from the L channel to the extracellular." Among them, the K+ and Na+ uniform motion and uniform acceleration motion mentioned in the article mean that the number of K+ and Na+ or the number of charges changes at a uniform speed or uniform acceleration, not the real motion mode of K+ and Na+ or charge entering and leaving neurons.By taking the opportunity of the official publication of this paper to corrigendum and declare that it will not affect the conclusions of the relevant papers.

The author made corrigendum to the article Conservation Law of Cell Bioelectricity Membrane Area and Ion Inequality Equation Based on Potassium Channel "Origami Windmill" Model of two clerical errors: Original content: The third paragraph of "1.2 Physical phenomena of cell membrane ion exchange", "Action potential, relative to K+, rises from −40mV to +40mV, which is the process of intracellular K+ from the L channel uniform deceleration flowing to the extracellular;" "the decrease from +40mV to −60mV, is a process of uniform deceleration of intracellular Na+ from the L channel to the extracellular." The "uniform deceleration" mentioned twice is changed to "uniform acceleration". Content after change: "Action potential, relative to K+, rises from −40mV to +40mV, which is the process of intracellular the amount of K+ from the L channel uniform acceleration flowing to the extracellular;" "the decrease from +40mV to −60mV, is a process of uniform acceleration of intracellular the amount ofNa+ from the L channel to the extracellular." Among them, the K+ and Na+ uniform motion and uniform acceleration motion mentioned in the article mean that the number of K+ and Na+ or the number of charges changes at a uniform speed or uniform acceleration, not the real motion mode of K+ and Na+ or charge entering and leaving neurons. By taking the opportunity of the official publication of this paper to corrigendum and declare that it will not affect the conclusions of the relevant papers.

Bioelectricity is the basic feature of life activities. Our brain produces brain waves all the time. Theoretically, brain waves should never disappear, and will always "float" in the universe. Brain wave is a kind of biological electromagnetic wave, which should be the carrier of mind, consciousness or soul. Its material basis is human body, brain, brain cells and ions inside and outside cells. It can be seen that the ancients said that there are gods three feet above the ground, as well as the so-called reincarnation, reincarnation, telepathy, and karma, there is a certain scientific reason.

Pericyclases, enzymes that catalyze pericyclic reactions, form an expanding family of enzymes that have biocatalytic utility. Despite the increasing number of pericyclases discovered, the Diels-Alder cyclization between a cyclopentadiene and an olefinic dienophile to form norbornene, which is among the best-studied cycloadditions in synthetic chemistry, has surprisingly no enzymatic counterpart to date. Here we report the discovery of a pathway featuring a norbornene synthase SdnG for the biosynthesis of sordaricin-the terpene precursor of antifungal natural product sordarin. Full reconstitution of sordaricin biosynthesis reveals a concise oxidative strategy used by Nature to transform an entirely hydrocarbon precursor into the highly functionalized substrate of SdnG for intramolecular Diels-Alder cycloaddition. SdnG generates the norbornene core of sordaricin and accelerates this reaction to suppress host-mediated redox modifications of the activated dienophile. Findings from this work expand the scopes of pericyclase-catalyzed reactions and P450-mediated terpene maturation.

The nitroreductase superfamily of enzymes encompasses many flavin mononucleotide (FMN)-dependent catalysts promoting a wide range of reactions. All share a common core consisting of an FMN-binding domain, and individual subgroups additionally contain one to three sequence extensions radiating from defined positions within this core to support their unique catalytic properties. To identify the minimum structure required for activity in the iodotyrosine deiodinase subgroup of this superfamily, attention was directed to a representative from the thermophilic organism Thermotoga neapolitana (TnIYD). This representative was selected based on its status as an outlier of the subgroup arising from its deficiency in certain standard motifs evident in all homologues from mesophiles. We found that TnIYD lacked a typical N-terminal sequence and one of its two characteristic sequence extensions, neither of which was found to be necessary for activity. We also show that TnIYD efficiently promotes dehalogenation of iodo-, bromo-, and chlorotyrosine, analogous to related deiodinases (IYDs) from humans and other mesophiles. In addition, 2-iodophenol is a weak substrate for TnIYD as it was for all other IYDs characterized to date. Consistent with enzymes from thermophilic organisms, we observed that TnIYD adopts a compact fold and low surface area compared with IYDs from mesophilic organisms. The insights gained from our investigations on TnIYD demonstrate the advantages of focusing on sequences that diverge from conventional standards to uncover the minimum essentials for activity. We conclude that TnIYD now represents a superior starting structure for future efforts to engineer a stable dehalogenase targeting halophenols of environmental concern.

In order to reasonably explain the phenomenon of cell bioelectricity, we proposed the conservation law of cell membrane area, established the ion inequality equation, and therefore paid attention to the mystery of "θ-τ". We researched and analyzed the "θ-τ" mystery, discussed the parity non-conservation in weak interactions, suggested possible experiments to test the parity non-conservation in weak interactions, and gave our research and analysis conclusions: The parity non-conservation in weak interactions, which is still a "conjecture"; The experimental scheme suggested in the papers by C. N. Yang et al. cannot determine whether the weak interaction can separate left and right, and it is impossible to directly answer whether θ and τ in the "θ-τ" mystery are the same particle; The Co60 β decay experiment such as C. S. Wu is a pseudo-mirror experiment, whether the experimental result violates parity conservation is only based on the assumption of C. N. Yang et al. In fact, experiments such as polarized Co60 did not overturn the so-called "law of parity conservation". The mirror image principle does not have any physical meaning, does not correspond to any physical conservation quantity, and cannot be destroyed by any physical experiment. In the process of turning "mirror symmetry" and "mirror asymmetry" into so-called physical "common sense" and scientific "facts" respectively, the methods of transformation are "stealing concepts" and "circular argumentation". The "θ-τ" mystery is a "man-made" mystery. θ and τ are two different particles, which may be the result of the same precursor particle being divided into two. The work of C. N. Yang, T. D. Lee, C. S. Wu et al. have brought quantum physicists from the "small black room" to the "bigger black room" or "smaller black room". The right and wise choice is to go back through the door that came in. With the development of science today, it is time for some contents to reform from the bottom.

Applying the K+ channel origami windmill model principle, the whole process of action potential generation of nerve fiber cells and cardiomyocytes is explained reasonably. Its core view is: cell action potential decline phase, dominated by influx of potassium ions. This is contrary to the core idea of the traditional theory—ionic theory. According to ionic theory, cell action potential decline phase, dominated by outflow of K+ ions. In the face of such two opposing views, the author traces back to the source, combing and commenting on the basic theoretical research on the production mechanism of cell bioelectricity generation and the results of classical basic experiments. And a series of enlightening viewpoints are produced:1. Ionic theory has its preexistence deficiency; 2. There are principle defects in GHK equation and H-H equation; 3. Ion channels may “same direction sharing”; 4. Sodium-potassium pumps, calcium pumps and so on May not exist. The thesis's Point is falsifiable. If “cell action potential decline stage, dominated by outflow of K+ ions” can be proved, all the points in this paper will not be true. On the contrary, the series of enlightening views in this paper will inevitably lead to a revolution in the field of life science. The viewpoint of this paper is not only closely related to the causes of human major diseases, treatment principles and methods, but also may have a profound impact on the future research direction of life science researchers. So the author suggests that scholars in the field of life science should re sort out the existing bioelectricity knowledge system—eliminate the false, save the true and clear the source, especially focusing on the papers published by Hodgkin and Huxley in 1952; At the same time, it is necessary to reevaluate the scientificity and scientific value of membrane theory, ionic theory, GHK equation and H-H equation, and put forward new theories based on Bernstein membrane theory, and establishing a new mathematical model of cell action potential.

The study of the action potential of cardiomyocytes is as important as the study of nerve fiber cells. The theoretical basis of the action potential of cardiomyocytes is the ionic theory formed by nerve fiber cells. Its core point of view: the decline phase of the action potential of cells is dominated by K+ outflow. Applying the principle of the K+ channel “origami windmill” model, the mechanism of action potential generation of nerve fiber cells has been reasonably explained, and a point contrary to the ion theory has been drawn: the phase of the action potential decline is dominated by K+ influx. Similarly, applying the principle of the K+ channel “origami windmill” model can also give a reasonable explanation for the mechanism of action potential generation of cardiomyocytes. The difference between cardiomyocyte action potential and nerve fiber cell: In addition to K+, Cl-, Na+, the process of generating cardiomyocyte action potential also involves the participation of Ca2+. Ca2+ inflow in the rising phase will shorten the depolarization time; Ca2+ inflow in the falling phase will prolong the repolarization time. K+, Ca2+ space substitution proportion: rotate into 2 K+, squeeze out 3 Ca2+, and 4 extra negative charges in the cell, the proportion is 2: 3: 4; Conversely, squeeze out 2 K+, rotate into 3 Ca2+, 4 extra positive charges the cells, the proportion is 3: 2: 4. Na+, Ca2+ space substitution proportion: rotate into 1 Na+, squeeze out 1 Ca2+, and 1 extra negative charges in the cell, the proportion is 1: 1:1; Conversely, rotate into 1 Ca2+, squeeze out 1 Na+, there is 1 more positive charge in the cell, and the proportion is 1: 1: 1. Application of “origami windmill” model principle, can reasonably explain the cardiomyocytes action potential generation mechanism, further proveding “origami windmill” the model theory, “the theory of dove-like particles”“the theory of braincell activation” of science, also proved that the viewpoint of “action potential descending phase, dominated by K+ internal current” is correct. The scientific and rational interpretation of the action potential of cardiomyocytes has far-reaching impact and great significance to the basic theoretical research and clinical practice of the heart from now on.

Reductive dehalogenation offers an attractive approach for removing halogenated pollutants from the environment, and iodotyrosine deiodinase (IYD) may contribute to this process after it can be eng...

Plasma electrolytic oxidation (PEO) coatings fabricated on 6061 aluminum alloy under different applied voltages (400 V, 500 V and 600 V) were used to investigate the plasma discharge induced ‘local over-growth’ characteristics across substrate/coating interface. The instant microdischarges across the local plasma channels induce the ‘micro molten pool’ products and then solidify rapidly to form ‘over-growth’ oxides region extending into substrate. A thin bonding layer composed of nanocrystalline and amorphous Al2O3 with a constant thickness of ~600 nm is always forming on the interface. The intact interface structure of the “local over-growth” characteristic patterns was presented perfectly by both microdischarge-sculpted substrate side ‘pits’ and electrochemically detached coating side ‘protrusions’. The patterns demonstrate that the over-growth ‘pits’ and ‘protrusions’ gradually grow up with increasing applied voltage, which is attributed to the larger discharge channels and molten pools caused by the increased size and intensity of plasma discharges. The speculative ‘over-growth’ mechanism of discharge event during plasma electrolytic oxidation process is described. This work provides insights to control ‘over-growth’ characteristics of substrate/coating interface by tailoring electrical parameters or electrolytes composition. And this strategy has a potential application to fabricate micro-nano structure or optimize outstanding performance of PEO coatings.

Iodotyrosine deiodinase (IYD) is unusual for its reliance on flavin and ability to promote reductive dehalogenation under aerobic conditions. As implied by the name, this enzyme was first discovered to catalyze iodide elimination from iodotyrosine for recycling iodide during synthesis of tetra- and triiodothyronine collectively known as thyroid hormone. However, IYD likely supports many more functions and has been shown to debrominate and dechlorinate bromo- and chlorotyrosines. A specificity for halotyrosines versus halophenols is well preserved from humans to bacteria. In all examples to date, the substrate zwitterion establishes polar contacts with both the protein and the isoalloxazine ring of flavin. Mechanistic data suggest dehalogenation is catalyzed by sequential one electron transfer steps from reduced flavin to substrate despite the initial expectations for a single two electron transfer mechanism. A purported flavin semiquinone intermediate is stabilized by hydrogen bonding between its N5 position and the side chain of a Thr. Mutation of this residue to Ala suppresses dehalogenation and enhances a nitroreductase activity that is reminiscent of other enzymes within the same structural superfamily.

[Objective] Verification and application of the theory of brain cell activation. [Methods] Applying Transcranial magnetoelectric encephalopathy treatment instrument to the treatment of Neurodegenerative disease. including but not limited to: Parkinson's disease and Alzheimer's disease. [Results] Transcranial magnetoelectric encephalopathy treatment instrument，its double - center, randomized, double - blind clinical trial has been carried out at the national clinical trial base，it proves that the instrument is safe and effective. This instrument is especially suitable for the treatment of the following diseases: 1. It is suitable for the treatment of mild to moderate Parkinson's disease and can significantly improve resting tremor, rigidity, bradykinesia in patients with Parkinson’s disease and other symptoms. 2. It is suitable for the treatment of mild and moderate Alzheimer's disease and vascular dementia and can improve the mental state, cognitive behavior and self-care ability of daily life. 3. It is suitable for the treatment of mild to moderate depression and can obviously improve the main symptoms of depression, sleep disorder, anxiety and so on. 4. It is suitable for the treatment of cerebral apoplexy sequelae, cerebrovascular dementia and brain atrophy, it can activate the brain cells in the state of inhibition and mobilize the potential energy of the brain. [Conclusions] The theory of brain cell activation，It is applicable to encephalopathy and not limited to encephalopathy,according to this theory, the subject of "physical disease science" can be created.

The theory of dove-like particles is a discussion on the reasons and mechanism of sporadic Alzheimer's disease, which was officially published on March 15, 2019. In this paper, the "basic content" of the theory of dove-like particles and the mechanism of action potential generation of Alan Hodgkin and Andrew Huxley are revised, the related contents of ion pump and sodium-potassium pump are deleted, and the discussion of " organic ions and acidity-alkalinity " is added, and at the end of the conclusion, the following contents are added: The Aβ hypothesis misleads the research direction of the world's mainstream brain scientists. All the work based on the beta hypothesis has been unsuccessful. It may even become the confirmed habits are hard to get rid of "soft underbelly" of some scientists and "stumbling block" to conquered Alzheimer's disease. Its little results which seeming to be right but not so in fact is completely negligible compared with the enormous cost of human beings. Therefore, we must completely deny and abandon the Aβ hypothesis from the strategic height and academic level. Trying to revision and supplement it to keep it reluctantly, it is not scientific and has no practical significance, it confuses the gap between theory and practice.

The mechanism of cell action potential was explained by using the principle of potassium channel "origami windmill" model. It is inferred that ion channels should include at least two categories: One kind of channel is "special ion channel", its structure is like an origami windmill model. All cations passing through this channel rotate into the interior from one-way, only in and no out. Compared with K+, they have two states of "open" and "closed", When they are "open", their aperture is not less than K+ diameter. When "closed", their aperture is smaller than K+ diameter, but not smaller than Na+ diameter. The other channel is the "universal ion channel". All Ions passing through this channel unidirectional flow too, only out and no in. Compared with K+, they have two states of "open" and "closed", When they are "open", their aperture is not less than K+ diameter. When "closed", their aperture is smaller than K+ diameter, but not smaller than Na+ diameter. This model reasonably explains the whole process of action potential occurrence, and supports Hodgkin, Huxley 's experimental the results of action potential. This model does not support their explanation of the mechanism of action potential generation in cells and the core ideas of "membrane theory" and "ion theory". It negates the selective filter atomic model and the propeller model established by MacKinnon et al. It is tiped that the main role of "sodium-potassium pump" or "ATPase" is not responsible for the transport of Na+ and K+ from the inside and outside of the cell and maintaining cell membrane potential. The channels through which ions enter and escape cells are independent. This suggests that most channels may be sharing in the same direction by other inorganic ions and organic molecules.

An enlightening viewpoint based on the theory of brain cell activation is to explore the pathogenesis and mechanism of sporadic Alzheimer's disease (AD) at molecular level by applying the principles of cell physics and biology. Its purpose is to coordinate and unify the existing theories of AD etiology and to solve the problems that have puzzled the research field of neurodegenerative diseases for a long time. Basic Contents: Excessive cations are transferred from extracellular to intracellular, and compete with potassium ions on the inner surface of the cell membrane. As a result, the action potential produced can not activate calcium channels normally, leading to abnormal apoptosis of brain cells. Amyloid plaques are the remains of abnormal apoptotic brain cells. Amyloid plaques are aggregated by van der Waals force and electrostatic attraction between amyloid plaques. The interstitium is amyloid protein. Brain cells consist of neurons, microglia and astrocytes in turn. Most of the spotted nuclei in the remains are cations. Applicable but not limited to neurodegenerative diseases such as Alzheimer's disease, physical means should be preferred to solve such diseases.

This is a new idea that based on effective treatment of Parkinson's disease and Alzheimer's disease with transcranial magnetoelectric stimulation technology, it can understand a hypothesis about voltage-gated Ca2+ channels is the best target for activation by physical means, basic content：Parkinson's disease , Alzheimer's disease etc. neuronal degeneration diseases, that closely related to physical-gated ion channels, which can be treated with physical means, activating neurotransmitters-energic neurons plays key roles in the treatment, and voltage-gated Ca2+ channels is the best target for physical means, the purpose is to induce Ca2+ inflowing and triggers neuronal axon terminals synaptic vesicles releasing neurotransmitters. The theory of brain cell activation sets forth the principle, method and purpose of treatment of the physical gated ion channel diseases such as Alzheimer's disease, Parkinson's disease and other neural degeneration diseases, and indicates that the attempt to treat these diseases using pharmaceutical and chemical approaches could shake our confidence in conquering the diseases, and the application of physical approaches or combined application of physical and chemical approaches in the treatment of some major encephalopathy may be our main research direction in the future.

China has been instituting national basic education curriculum reforms since 2001. This study provides an updated understanding of present-day, rural primary school music education in Northeastern China’s Tonghua region. A total of 126 rural primary music teachers and 674 students from 28 primary schools in the region were surveyed using a questionnaire. In addition, teachers were interviewed about the factors responsible for the current situation. The findings reveal that primary music education in the rural areas of North-east China is suffering from a number of problems including the inadequate provision of resources by local government, teachers’ lack of professionalism in terms of music education, and an inadequate understanding of the new curriculum reforms and rationales. The measures recommended to improve the current situation in the region may also provide insights that would prove useful for global consideration.