Search

Item does not contain fulltext Dendritic cells (DC) are unique in their ability to present antigen to naive T cells, and therefore play a central role in the initiation of immune responses. Characterization of DC-specific genes may help to unravel the mechanism underlying their potent antigen presenting capacity. Here we describe the identification of a novel transcript, isolated by random sequencing of a cDNA library prepared from monocyte-derived DC, which we termed DC-specific transmembrane protein (DC-STAMP). DC-STAMP is specifically expressed by DC, and not in a panel of other leukocytes or non-hematopoietic cells. Interestingly, DC-STAMP was also detected in activated but not resting blood DC. The DC-STAMP transcript encodes a 470-amino acid protein containing seven putative transmembrane domains. Expression of a DC-STAMP-GFP fusion protein in 293 cells indicates that DC-STAMP is expressed at the cell surface, and has an intracellular C terminus. Surprisingly, no sequence homology was found with any other protein or multimembrane-spanning receptor. Therefore, we propose that DC-STAMP is a novel DC-specific multimembrane-spanning protein, representing a new group of transmembrane proteins.

Contains fulltext : 24093___.PDF (Publisher’s version ) (Open Access)

Human mesenchymal stem cells have a high potential in regenerative medicine. They can be isolated from a variety of adult tissues, including bone marrow, and can be differentiated into multiple cell types of the mesodermal lineage, including adipocytes, osteocytes, and chondrocytes. Stem cell differentiation is controlled by a process of interacting lineage-specific and multipotent genes. In this chapter, the authors use full genome microarrays to explore gene expression profiles in the process of Osteo-, Adipo-, and Chondro-Genic lineage commitment of human mesenchymal stem cells.

Item does not contain fulltext

Item does not contain fulltext

Contains fulltext : 250022.pdf (Publisher’s version ) (Open Access)

Contains fulltext : 249803.pdf (Publisher’s version ) (Open Access)

Aberrant expression of the platelet-derived growth factor a-receptor (PDGFRA) gene has been associated with various diseases, including neural tube defects and gliomas. We have previously identified 5 distinct haplotypes for the PDGFRA promoter region, designated H1, H2 alpha, H2 beta, H2 gamma and H2 delta. Of these haplotypes H1 and H2 alpha: are the most common, whereby H1 drives low and H2 alpha high transcriptional activity in transient transfection assays. Here we have investigated the role of these PDGFRA promoter haplotypes in gliomagenesis at both the genetic and cellular level. In a case-control study on 71 glioblastoma patients, we observed a clear underrepresentation of H1 alleles, with pH1 = 0.141 in patients and pH1 = 0.211 in a combined Western European control group (n = 998, p < 0.05). Furthermore, in 3 out of 4 available H1/H2 alpha heterozygous human glioblastoma cell lines, H1-derived mRNA levels were more than 10-fold lower than from H2 alpha, resulting at least in part from haplotype-specific epigenetic differences such as DNA methylation and histone acetylation. Together, these results indicate that PDGFRA promoter haplotypes may predispose to gliomas. We propose a model in which PDGFRA is upregulated in a haplotype-specific manner during neural stem cell differentiation, which affects the pool size of cells that can later undergo gliomagenesis. (C) 2008 Wiley-Liss, Inc.

Item does not contain fulltext 44th Annual meeting of The American Society for Cell Biology, 04 december 2004

Item does not contain fulltext Aio meeting

Item does not contain fulltext NOW meeting

Item does not contain fulltext

Item does not contain fulltext

Contains fulltext : 129298.pdf (Publisher’s version ) (Open Access)

Contains fulltext : 129324.pdf (Publisher’s version ) (Closed access)

Item does not contain fulltext

Contains fulltext : 129340.pdf (Publisher’s version ) (Closed access)

Item does not contain fulltext

Contains fulltext : 129339.pdf (Publisher’s version ) (Closed access)

Item does not contain fulltext

Item does not contain fulltext

Contains fulltext : 120796.pdf (Publisher’s version ) (Closed access) 5 p.

Item does not contain fulltext

Item does not contain fulltext

Item does not contain fulltext

Item does not contain fulltext

Item does not contain fulltext

Item does not contain fulltext

Item does not contain fulltext

Contains fulltext : 249386.pdf (Publisher’s version ) (Open Access)

Item does not contain fulltext

Contains fulltext : 100592.pdf (Publisher’s version ) (Open Access) Radboud Universiteit Nijmegen, 13 november 2012 Promotores : Zoelen, E.J.J. van, Gielen, C.C.A.M. Co-promotor : Theuvenet, A.P.R. 190 p.

Contains fulltext : 74880.pdf (Publisher’s version ) (Open Access) RU Radboud Universiteit Nijmegen, 12 januari 2010 Promotor : Zoelen, E.J.J. van 243 p.

Contains fulltext : 32053_humaexani.pdf (Publisher’s version ) (Open Access) Contains fulltext : 72481.pdf (Publisher’s version ) (Open Access) Radboud Universiteit Nijmegen, 17 januari 2008 Promotor : Pruijn, G.J.M. Co-promotores : Zoelen, E.J.J. van, Heck, A.J.R. van, Hendriks, W.J.A.J. 140 p.

Contains fulltext : 30023.pdf (Publisher’s version ) (Open Access) The frequently occurring bone disorder osteoporosis is characterized by a strong increase in bone fracture risk, caused by a dramatically disturbed balance in the activity of the cells that degrade bone (osteoclasts) and cells that synthesize new bone (osteoblasts). Therapies against osteoporosis are currently based on inhibition of osteoclast activity in order to reduce the amount of bone resorption. An alternative strategy would be to stimulate new bone formation by enhancing the activity of osteoblasts. The main objective of this thesis was to identify genes of which expression and function are specifically associated with the differentiation of mesenchymal stem cells into osteoblasts. Therefore, we used microarray-based gene expression profiling to study the transcriptome of differentiating osteoblasts during in vitro cell culture as well as in their physiological context during skeletogenesis. The analyses revealed many genes of which expression in osteoblasts had not previously been detected. Novel markers of osteoblast differentiation and bone development were identified as well as putative in vivo target genes of the transcription factor Runx2 which is a key regulator of skeletal development. One of the markers identified encodes the protein Wif1 (Wnt Inhibitory Factor 1), which was shown to be specifically expressed in osteoblasts during embryonic development. Typically, genes encoding antagonists of the Wnt signalling pathway were strongly expressed during late stages of osteoblast differentiation and implicate an important role of Wnt antagonism in the maturation of osteoblasts. Given the role of Wnt signalling in the maintenance of bone mass, this class of proteins may be potential drug targets to treat bone disorders. This thesis shows that the combination of microarray technology and bioinformatic analyses has provided new possibilities to the understanding of bone biology. Consequently, our studies have contributed to a more detailed knowledge on osteoblast differentiation and provide focus points to develop strategies that will prevent and treat bone disorders. Radboud University, Applied Biology, 04 juni 2007 Promotores : Olijve, W., Zoelen, E.J.J. van Co-promotor : Dechering, K.J. 264 p.

Contains fulltext : 30196.pdf (Publisher’s version ) (Open Access) Radboud university, Cell Biology, 11 juni 2007 Promotor : Zoelen, E.J.J. van 113 p.

Contains fulltext : 19562.pdf (Publisher’s version ) (Open Access) The DNA sequences of many genomes have been elucidated in the last years. The challenge now is to predict first the genotype and then the phenotype from the genomic sequences. To predict expression patterns of the genes located on the genome, not only regulation at the level of transcription, but also at the level of translation is important. To obtain more information about regulation of transcription, the promoter and its regulatory elements need to be mapped. In this thesis a conventional search for known and active regulatory elements in the B2-crystallin promoter was performed. For the intergenic region between aB-crystallin and HspB2, an alternative approach was taken to determine which elements are likely to be important for gene regulation, and the evolutionary conservation of sequence elements in this region was studied. Alignments of this region of many species lead to the confirmation of importance of known regulatory elements and the finding of new sites. A good example of regulation of gene expression mediated by regulation of translation, is translation after stress. During and after heat shock, cap-dependent translation is inhibited, while cap-independent translation can still take place. After heat shock translation of (s)Hsps is increased, these (s)Hsps are thought to protect cells from stress. We show that two of the sHsps, aB-crystallin and Hsp27 protect cap-dependent, but not cap-independent translation after heat shock. Inhibition of translation is thought to be mediated by a decrease in activity of the translation factors. However, the protection of translation mediated by sHsps does not seem to affect translation factors directly, but overexpression of aB-crystallin seems to increase the dispersal of stress granules that contain the stalled translation initiation complexes. We propose that aB-crystallin, probably together with other Hsps, resolubilizes the stress granule components and that translational thermotolerance is a cooperative effect of different Hsps. RU, Biochemistry, 07 januari 2005 Promotores : Jong, W.W.W. de, Zoelen, E.J.J. van Co-promotor : Lubsen, N.H. 142 p.

Contains fulltext : 32614.pdf (Publisher’s version ) (Open Access) RU Radboud Universiteit Nijmegen, 26 oktober 2005 Promotores : Olijve, W., Zoelen, E.J.J. van 156 p.