Search

SOX9 is an essential transcriptional regulator of cartilage development and homeostasis. In humans, dysregulation of SOX9 is associated with a wide spectrum of skeletal disorders, including campomelic and acampomelic dysplasia, and scoliosis. The mechanism of how SOX9 variants contribute to the spectrum of axial skeletal disorders is not well understood. Here, we report four novel pathogenic variants of SOX9 identified in a large cohort of patients with congenital vertebral malformations. Three of these heterozygous variants are in the HMG and DIM domains, and for the first time, we report a pathogenic variant within the transactivation middle (TAM) domain of SOX9. Probands with these variants exhibit variable skeletal dysplasia, ranging from isolated vertebral malformation to acampomelic dysplasia. We also generated a Sox9 hypomorphic mutant mouse model bearing a microdeletion within the TAM domain (Sox9Asp272del). We demonstrated that disturbance of the TAM domain with missense mutation or microdeletion results in reduced protein stability but does not affect the transcriptional activity of SOX9. Homozygous Sox9Asp272del mice exhibited axial skeletal dysplasia including kinked tails, ribcage anomalies, and scoliosis, recapitulating phenotypes observed in human, while heterozygous mutants display a milder phenotype. Analysis of primary chondrocytes and the intervertebral discs in Sox9Asp272del mutant mice revealed dysregulation of a panel of genes with major contributions of the extracellular matrix, angiogenesis, and ossification-related processes. In summary, our work identified the first pathologic variant of SOX9 within the TAM domain and demonstrated that this variant is associated with reduced SOX9 protein stability. Our finding suggests that reduced SOX9 stability caused by variants in the TAM domain may be responsible for the milder forms of axial skeleton dysplasia in humans.

ObjectiveTo analyze the application value of two postural fixation techniques.(styrofoam combined with head mask and fixed headrest combined with head mask) in intracranial tumor radiotherapy via cone beam computed tomography (CBCT).MethodsThis study included 104 patients with intracranial tumors undergoing radiotherapy. The patients were divided into two groups: Group A (54 cases with styrofoam fixation) and Group B (50 cases with fixed headrest fixation). The positional deviation in 3D space between the two groups was compared using CBCT. The set-up errors were expressed as median (25th percentile, 75th percentile)or M(p25, p75) since the set-up errors in all directions were not normally distributed,The Mann-Whitney U test was performed.ResultsThe age and gender of patients in the two groups were not significantly different. The set-up errors of A in lateral (X), longitudinal (Y), vertical (Z), and yaw(Rtn) axes were 1.0 (0,1) mm, 1.0 (0,1) mm, 1.0 (0,2) mm, and 0.4 (0.1, 0.8) degrees, respectively while the set-up errors of B were 1.0 (0,1) mm, 1.0 (1,2) mm, 1.0 (0,2) mm, and 0.5 (0.15,0.9) degrees, respectively. Moreover, patients in the styrofoam group had significantly smaller set-up errors in the Y-axis than patients in the headrest group (p=0.001). However, set-up errors in the X, Z, and Rtn axes were not significantly different between the two groups. The expansion boundaries of the target area in the X, Y, and Z directions were 1.77 mm, 2.45 mm, and 2.47 mm, respectively. The outer expansion boundaries of the headrest group were 2.03 mm, 3.88 mm, and 2.57 mm in X, Y, and Z directions, respectively. The set-up times of groups A and B were (32.71 ± 5.21) seconds and (46.57 ± 6.68) seconds, respectively (p=0.014). Patients in group A had significantly better comfort satisfaction than patients in group B (p=0.001).ConclusionStyrofoam plus head thermoplastic mask body fixation technique has a higher positional accuracy in intracranial tumor radiotherapy than headrest plus head thermoplastic mask fixation. Besides, styrofoam plus head thermoplastic mask body fixation technique is associated with improved positioning efficiency, and better comfort than headrest plus head thermoplastic mask fixation, and thus can be effectively applied for intracranial tumor radiotherapy positioning.

With the rapid expansion of power system scale, demand response business is promoted to develop. More and more demand response terminals are connected to the smart grid, smart grid is an intelligent system that allows the grid to effectively perform its functions. Its data can be used in intelligent decision-making during grid operation, which may be attacked by hackers in practical applications, causing security problems of demand response terminals of the power network. The security feedback trust model establishes trust relationship through trust mechanism, which can effectively ensure the security of interaction between nodes and demand response terminals of the smart grid. Therefore, a security feedback trust model of power network demand response terminal triggered by hacker attacks is proposed. Analyze the role of smart grid in power grid, and use convolutional neural network in artificial intelligence technology to enhance the flexibility of smart grid. Aiming at the security problem of the demand response terminal of the power network being attacked by hackers, based on the trust theory, the security feedback trust model of the demand response terminal of the power network is designed through the main services provided by the security feedback trust model, the trust information storage of the power network nodes and the summary of the main work. Establish the identity trust relationship, adopt the distributed verifiable signature scheme, update the power grid node certificate, update the identity trust relationship, and revoke the identity trust relationship based on the trust evaluation and threshold value to prevent hackers from attacking the power grid demand response terminal. Based on information theory, trust is established and measured. Entropy is used to represent the trust value. Behavior trust evaluation and composition mechanism are introduced into the security feedback trust model of power network demand response terminals to achieve the credibility of identity and behavior among power network nodes. The experimental results show that the proposed method can judge the hacker attacks, reduce the impact of hacker attacks on the trust of power grid nodes, and improve the interaction security between power grid demand response terminals and power grid nodes.

detailed theoretical calculations and simulations

The chest X-ray is often utilized for diagnosing common thoracic diseases. In recent years, many approaches have been proposed to handle the problem of automatic diagnosis based on chest X-rays. However, the scarcity of labeled data for related diseases still poses a huge challenge to an accurate diagnosis. In this paper, we focus on the thorax disease diagnostic problem and propose a novel deep reinforcement learning framework, which introduces prior knowledge to direct the learning of diagnostic agents and the model parameters can also be continuously updated as the data increases, like a person's learning process. Especially, 1) prior knowledge can be learned from the pre-trained model based on old data or other domains' similar data, which can effectively reduce the dependence on target domain data, and 2) the framework of reinforcement learning can make the diagnostic agent as exploratory as a human being and improve the accuracy of diagnosis through continuous exploration. The method can also effectively solve the model learning problem in the case of few-shot data and improve the generalization ability of the model. Finally, our approach's performance was demonstrated using the well-known NIH ChestX-ray 14 and CheXpert datasets, and we achieved competitive results. The source code can be found here: \url{https://github.com/NeaseZ/MARL}.

Additional File 1: Supplementary Table S1-S4

Hypoxia alters eating behavior in different animals. In C. elegans, hypoxia induces a strong food leaving response. We found that this behavior was independent of the known O2 response mechanisms including acute O2 sensation and HIF-1 signaling of chronic hypoxia response. Mutating egl-3 and egl-21, encoding the neuropeptide pro-protein convertase and carboxypeptidase, led to defects in hypoxia induced food leaving, suggesting that neuropeptidergic signaling was required for this response. However, we failed to identify any neuropeptide mutants that were severely defective in hypoxia induced food leaving, suggesting that multiple neuropeptides act redundantly to modulate this behavior.

This paper presents and analyses a Constraint Energy Minimization Generalized Multiscale Finite Element Method (CEM-GMsFEM) for solving single-phase non-linear compressible flows in highly heterogeneous media. The construction of CEM-GMsFEM hinges on two crucial steps: First, the auxiliary space is constructed by solving local spectral problems, where the basis functions corresponding to small eigenvalues are captured. Then the basis functions are obtained by solving local energy minimization problems over the oversampling domains using the auxiliary space. The basis functions have exponential decay outside the corresponding local oversampling regions. The convergence of the proposed method is provided, and we show that this convergence only depends on the coarse grid size and is independent of the heterogeneities. An online enrichment guided by \emph{a posteriori} error estimator is developed to enhance computational efficiency. Several numerical experiments on a three-dimensional case to confirm the theoretical findings are presented, illustrating the performance of the method and giving efficient and accurate numerical.

ObjectiveSepsis Associated Encephalopathy (SAE) is a common complication in critically ill patients and perioperative period, but its pathogenesis is still unclear. This study aimed to explore the effect of the HIF-1α (hypoxia-inducible factor-1α)/BNIP3L (Bcl-2/adenovirus E1B 19-kDa interaction protein) signaling pathway on SAE.MethodsC57BL/6J male mice were divided into four groups, using a random number table method: control group, sham group, sepsis group, sepsis+HIF-1α activity inhibitor (echinomycin) group. Sepsis was induced by cecal ligation and puncture (CLP). At 24 h after surgery, brain tissue was sampled. HE was staining to observe changes in the hippocampus structure. Fluoroscopy observes changes in mitochondrial structure. Western blot, QT-PCR, and immunofluorescence were used to assess the amount of expression of HIF-1α and BNIP3L in the hippocampus and mitochondrion of hippocampus neurons. Observation of neuronal apoptosis by TUNEL staining. Seven days after surgery, mice were tested in a Morris water maze test to assess cognitive function after CLP.ResultsOur results show that CLP-induced hippocampus-dependent cognitive deficits were accompanied with increased HIF 1a and decreased BNIP3L, increased protein levels of TNF-α, IL-6, and IL-β, and damage to mitochondrial structures and neuronal apoptosis in the hippocampus. In addition, administration of echinomycin rescues cognitive deficits, ameliorates HIF-1α and BNIP3L-mediated neuronal pyroptosis and damaged mitochondrial structures, and decreases the expression of TNF-α and IL-6 in the hippocampus.ConclusionsHIF-1α and the BNIP3L promote mitochondrial damage, and neuronal apoptosis and the expression of inflammatory factors may be the mechanism of SAE in critically ill patients and perioperative period

Background: Grifola frondosa is a type of edible medicinal mushroom with abundant proteins. Selenium (Se) is an essential micronutrient for human. Many animal experiments and clinical studies had indicated that Se plays an important role in diverse physiologic actions. Most inorganic selenium compounds are toxic, and the lowest lethal dose is relatively small. Peptide-Se chelate can probably be dietary supplements in functional foods for humans with Se deficiency. Methods: In this study, a specific tripeptide Arg-Leu-Ala (RLA) with strong Se-chelating capacity was purified from Grifola frondosa through ultrafiltration, reversed-phase HPLC and gel filtration chromatography. The UV, SEM, XRD, 1H NMR spectra are shown to provide more information about characterization of RLA-Se chelates. The bioavailability of RLA-Se chelate in Caco-2 cell line was investigated by using human colon cancer Caco-2 cells as model. iTRAQ comparative proteomics approach were used to identify the differentially expressed proteins. Results: The Se binding capacity of RLA was 84.47 ± 1.21 mg g−1. The results of UV, X-ray diffraction (XRD), 1H NMR and SEM structure analysis showed that the binding of selenium in the hydrolysate of Grifola frondosa protein was successful, and the amino and carboxyl groups of RLA were involved in the coordination of Se, which was the main site of chelation. The results of absorption of RLA-Se chelate in Caco-2 cells showed that RLA-Se chelate could be used as selenium supplement source. Using iTRAQ comparative proteomics approach, 40 proteins found significant. RLA-Se treatment had been demonstrated to present a higher accumulation of Se compared with control treatment and show an effective absorption by Caco-2 with the result that E3 protein performed up regulation. RLA-Se may play roles in cell cycle and apoptosis as an essential micronutrient. To sum up, our research results show that Grifola polypeptide-Se chelate is a promising multifunctional organic selenium product, which can be used as a new functional supplement for selenium deficiency.

Multicolour photoluminescence has attracted extensive interest in the past decades due to its potential applications in anticounterfeiting and colour barcoding. However, it remains a great challenge to heterogeneously tune the luminescence colour in a single metal–organic framework (MOF) crystal/particle at bulk scale. Here, macroscopic colour multiplexing showing discernable emissions to naked eyes, has been first achieved on a new non-luminescent Ce3+-MOF crystal via efficient step-by-step postsynthetic transmetalation. The presence of a clear demarcation line between dual emissions has been validated by the luminescence lifetimes. Furthermore, multiplex emission can be selectively and rapidly turned-off via an easily performed post synthetic chemical treatment.

In light of growing concerns about the formation of nitrogen-based disinfection byproducts (N-DBP) and the possible contribution from the use of quaternary-ammonium-containing flocculants, there is growing interest in the alternative use of quaternary phosphonium salts, which have been reported to have a lower DBP formation potential, stronger cationic properties, lower cytotoxicity, and greater stability. In this study, the performance of N-free quaternary-phosphonium-modified starch flocculants (S-BTP), synthesized through a facile one-step method using commercially available raw materials, in the treatment of bacteria-laden waters (E. coli as the model bacteria) was assessed in both jar tests and a bench-scale continuous-flow flocculation–sedimentation–ultrafiltration process. In jar tests, the effects of the cationic degree of substitution (DS) and dosage of the flocculant, solution pH, and presence of model contaminants on treatment performance were studied. One particular flocculant (S-BTP3), with a DS of 19.3%, displayed high removal efficiencies of E. coli, turbidity, and UV254 from water, comparable with those of ammonium-based analogues and conventional alum, via a combination of charge attraction, polymer bridging, and antibacterial effects. S-BTP3 also possessed better bactericidal properties (99.4% of E. coli killed) than alum (41.4% killed) and did not cause the release of intracellular substances into the treated water. In the continuous-flow flocculation–sedimentation–UF tests, S-BTP3 was superior to alum in the flocculation and antibacterial performance, and in mitigating UF membrane fouling. The results have clearly demonstrated the multiple benefits of the use of N-free cationic starch flocculants in water treatment as an alternative to conventional chemicals.

Asymmetric mode transformation in waveguide is of great significance for on-chip integrated devices with one-way effect, while it is challenging to achieve asymmetric nonlinear-mode-conversion (NMC) due to the limitations imposed by phase-matching. In this letter, we theoretically proposed a new scheme for realizing asymmetric NMC by combining frequency-doubling process and periodic PT symmetric modulation in an optical waveguide. By engineering the one-way momentum from PT symmetric modulation, we have demonstrated the unidirectional conversion from pump to second harmonic with desired guided modes. Our findings offer new opportunities for manipulating nonlinear optical fields with PT symmetry, which could further boost more exploration on on-chip nonlinear devices assisted by non-Hermitian optics., Comment: to be published in Frontiers of Physics

Detailed theoretical calculations and analyses

Additional file 1: Fig. S1. Flow cytometry estimation of the genome size for A. rhodopa female and male. Fig. S2. TE subclass landscapes of two species. Fig. S3. Repeat profiles of the remaining 31 shared TEs between two species. Fig. S4. Low-level piRNA silencing in A.rhodopa ovary. Fig. S5. Correlation analysis of K2P distance of TE with TE abundance and piRNA abundance.

Lake sediment and algal sludge with large output posed significant environmental risks. In this work, an idea of co-utilization of both solid wastes for the production of ceramsite (a sort of porous lightweight aggregates as building materials) was proposed and validated for the first time. The treatment process contained a dewatering step by a flocculation-pressure filtration method, and a sintered ceramsite preparation step. Effects of flocculant type and dosage on the dewatering performance were studied in the first step. An environmental-friendly amphoteric starch flocculant with a dosage of 12 mg/(g dried sample) was found to achieve the best dewatering performance. Effects of raw material mass ratio, sintering temperature and time in the second step were investigated. Under the optimal conditions (60 wt% of dewatered sediment; 20 wt% of dewatered algal sludge; 20 wt% of additives (fly ash: calcium oxide: kaolin = 2:1:2); sintering temperature: 1100 °C; time: 35 min), the obtained ceramsite met the Chinese National Standard as a qualified building material, with reliable environmental safety according to the leaching results for both heavy metals and microcystins. Both environmental and economic benefits of the proposed treatment were assessed. The process completely followed the rules of “reduction, harmlessness and resource utilization” for solid waste treatment and disposal; Meanwhile, the profit of the proposed ceramsite production could be more than 2.3 US dollar/m3. The co-utilization method in this work acted as a good example for the comprehensive management of solid wastes in water-rich areas.

Background: The gene-based real-time PCR method for identification of Campylobacter jejuni is more simple, rapid and accurate than the traditional biochemical method. Objective: A performance validation of the TadpoleTMCampylobacter jejuni Real-Time PCR Identification Kit was performed. Method: The assay uses TaqMan Real-time PCR technology to amplify target genes from isolated colonies. Bacterial deoxyribonucleic acid (DNA) from inclusivity and exclusivity organisms cultured on Columbia Blood Agar, Campy-Cefex agar and modified Charcoal Cefoperazone Deoxycholate was extracted and analyzed on three instruments: Applied Biosystems (ABI) 7500 Fast, ABI StepOne Plus and Bio-Rad CFX96. Results: When 57 distinct strains of C. jejuni were tested for inclusivity, all 57 strains produced positive results on the three instruments. In exclusivity testing, all 35 strains of related organisms, including 7 non-target Campylobacter strains and other common species, produced negative results on the three instruments. The Independent Laboratory validation consisting of an inclusivity and exclusivity evaluation for 10 C. jejuni isolates and 10 nontarget Campylobacter isolates also showed 100% expected results on the three instruments. In addition, in robustness testing, small, deliberate changes to the assay parameters, including cell suspension turbidity, heat lysis time, and DNA template volume in the PCR reaction, did not affect the kit performance. Finally, the combined lot-to-lot and stability study on both the ABI 7500 Fast and the ABI StepOne Plus showed that the 11 C. jejuni strains and 5 nontarget Campylobacter strains can be correctly identified by the three independently manufactured, lots and it supported a shelf life of 9 months when stored at –20°C. Conclusions: The Tadpole method offers a rapid, accurate, and robust alternative for C. jejuni identification. Highlights: Rapid and accurate method to identify C. jejuni, which has a good robustness and high stability. It is flexible and offers the advantages of reduced labor and time saving.

In this study, we aimed to evaluate the effect of Ganoderma lucidum protease hydrolysate (GLPH) on lipid metabolism and intestinal flora in rats fed on a high-fat diet. Our research shows that GLPH reduces triglycerides, total cholesterol, low-density lipoprotein cholesterol, alanine aminotransferase and aspartate aminotransferase levels, but increases high-density lipoprotein cholesterol in the serum and liver. Besides, GLPH has hypolipidemic effects, such as sterol regulatory element-binding transcription factor 1C, acetyl-coenzyme A carboxylase at the liver mRNA level were down-regulated, 5′-adenosine monophosphate (AMP)-activated protein kinase, acyl-CoA oxidase, farnesoid X receptor and peroxisome proliferator-activated receptor α at the liver mRNA level was upregulated. GLPH treatment changes the composition of the intestinal flora and reduces the relative abundance of potentially harmful bacteria, such as Alistipes, Clostridium. Spearman's correlation analysis showed that changes in faecal microbiota are related to biochemical indicators. In summary, our research indicates that GLPH can improve lipid metabolism disorders, regulate the structure of intestinal microbes, and can be used as a lipid-lowering functional food.

Objective The aim of this study was, first, to ascertain the efficacy of radioiodine therapy (RIT) for Graves’ disease (GD) based on a calculated-dose regime and, second, to determine the value of 99mTc-pertechnetate thyroid scintigraphy in predicting the clinical outcomes of RIT. Methods One hundred and thirty nine consecutive GD patients who underwent RIT using a calculated-dose method in our hospital from January 2015 to September 2015 were retrospectively evaluated. Radioiodine dose was calculated based on the Marinelli's formula. 99mTc-pertechnetate uptake, age, gender, thyroid mass, duration of the disease, previous antithyroid drugs treatment, serum levels of TSH, FT3 and FT4, a positive rate of TPOAb and Anti-TRAb, radioiodine dose and follow-up were evaluated as potential interference factors for RIT success. Results One hundred and eight (77.7%) GD patients including 71 (51.1%) euthyroid and 37 (26.6%) hypothyroid were successful, but 31 (22.3%) remained hyperthyroid (treatment failure). Significant differences were found between the treatment success group and the failure group in 99mTc-pertechnetate uptake (p Conclusion Our study has shown that a calculated radioiodine dose is effective in treating GD patients with a consequent low rate of hypothyroid. A 99mTc-pertechnetate uptake above 18.4% is a significant predictor of treatment failure and these patients should receive a higher radioiodine dose in this scenario.

Supplemental material, sj-docx-6-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-3-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-2-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-6-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-4-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-7-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-3-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-5-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-4-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-5-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-7-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-1-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-2-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

Supplemental material, sj-docx-1-hkj-10.1177_10249079211040971 for Current situation and expectation of emergency medicine education for 8-year Doctor of Medicine degree program in China by Zengzheng Ge, Shi Feng, Xiangning Liu, Shigong Guo, Yanxia Gao, Xin Lu, Shiyuan Yu, Lina Zhao, Di Shi, Jihai Liu, Huadong Zhu and Yi Li in Hong Kong Journal of Emergency Medicine

The system has the uncertainty of electromagnetic power fluctuation or the difficulty of detection. It will affect the stability of the system. In this paper, a new sliding mode surface and sliding mode controller are designed. It will solve the external disturbance of interconnected power system problem. Firstly, a network system model is introduced. Then a fast terminal sliding surface control algorithm is designed according to the structural characteristics of the system. The uncertain parameters in the network system are estimated in advance. Based on the Lyapunov method, the stability and fast convergence of the network system are proved. Finally, the method is applied to the stability control of second-order interconnected power systems. Numerical simulation results are given to illustrate correctness and effectiveness of the introduced sliding mode controller, and are given to make the interconnected systems reach a stable state quickly and run smoothly.

Modeling flows in fractured porous media is important in applications. One main challenge in numerical simulation is that the flow is strongly influenced by the fractures, so that the solutions typically contain complex features, which require high computational grid resolutions. Instead of using uniformly fine mesh, a more computationally efficient adaptively refined mesh is desirable. In this paper we design and analyze a novel residual-type a posteriori error estimator for staggered DG methods on general polygonal meshes for Darcy flows in fractured porous media. The method can handle fairly general meshes and hanging nodes can be simply incorporated into the construction of the method, which is highly appreciated for adaptive mesh refinement. The reliability and efficiency of the error estmator are proved. The derivation of the reliability hinges on the stability of the continuous setting in the primal formulation. A conforming counterpart that is continuous within each bulk domain for the discrete bulk pressure is defined to facilitate the derivation of the reliability. Finally, several numerical experiments including multiple non-intersecting fractures are carried out to confirm the proposed theories., Comment: 20 pages, 16 figures. arXiv admin note: text overlap with arXiv:2005.10955

In 2018, the United States Department of Agriculture (USDA-ARS), the Clemson University, and the University of California jointly announced 40 new varieties of blueberry, including 12 varieties of northern highbush blueberry, 21 varieties of southern highbush blueberry, and 7 varieties of ornamental blueberry. Based on the analysis of the comprehensive characteristics of the announced blueberry varieties, this paper summarizes the current development trend of global blueberry breeding. The results have been shown that: 1) the cultivation of southern highbush blueberry is still the main direction of blueberry breeding, and the number of new ornamental blueberry varieties has increased. 2) The main breeding direction for northern highbush blueberry is to cultivate new varieties with early maturity, large fruit, hard texture, and good storability. 3) The breeding trend of blueberries in the southern highbush blueberry is mainly focused on cultivating new cultivars with the low chilling requirement and have comprehensive characteristics such as early maturity, large fruit, and good fruit quality. 4) The main direction of ornamental blueberry breeding is to pay attention to the diversification of fruit color and the leaf color that changes with the season for use in Garden potted plants and landscaping. 5) In recent years, China has made rapid progress in blueberry breeding except for the traditional breeding countries such as Europe and America. The breeding trend described in this paper will point out the direction for blueberry breeding in China in the future and have important practical reference value.

A novel heteropolysaccharide from Grifola frondosa named GFP-W has been isolated and purified by DEAE Sephadex A-52 chromatography. Gas chromatography, fourier transform infrared spectroscopy, one-dimensional (1H- and 13C-) and two-dimensional (1H-1H COSY, 1H-13C HSQC, and 1H-13C HMBC) nuclear magnetic resonance spectroscopy were used to characterize its structure. The average molecular weight of GFP-W was 66.1 kDa. GFP-W mainly contained four kinds of linkage type units as β-D-GlcpA→, 1,2,6-α-Gal, →2)-α-Manp→, and →3)-α-L-Fucp-(1→. It could significantly increase the uptake of glucose in dexamethasone induced insulin resistant HepG2 cells by improving the mRNA and protein expression of insulin receptor substrate 1, phosphatidylinositol-3-kinase, and glucose transporter 4 upregulation and c-Jun N-terminal Kinase 1 downregulation. Moreover, antidiabetic activities of GFP-W were associated with significant changes in the extent of protein lysine acetylation, crotonylation, and succinylation levels. Our results provide a new hypoglycemic therapeutic role and an in-depth analysis on molecular mechanisms upon polysaccharides from G. frondosa.

The physicochemical characteristics and in vitro antioxidant and in vivo anti-ageing activities of partial purified Chlorella pyrenoidosa polysaccharides (PCPPs) were investigated. The building blocks of PCPPs were mainly composed of D-glucose, D-galactose and D-mannose. The average molecular weight of PCPPs was 9,950 Da. In vitro antioxidant activity assays showed that PCPPs could effectively scavenge hydroxyl, 1,1-diphenyl-2-picrylhydrazyl, and superoxide radicals, with stronger effect on hydroxyl radicals. Furthermore, the mean lifespan of the male and female Drosophila melanogaster was extended by 11.5% and 10.6%, respectively. This was accompanied by an increase in the total activity of the endogenous antioxidant enzymes, superoxide dismutase, glutathione peroxidase, and catalase in young or old D. melanogaster administered with PCPPs. Moreover, a gender-dependent difference was observed both in lifespan and antioxidant enzyme activities in D. melanogaster. The results indicated that C. pyrenoidosa polysaccharides are potential natural antioxidants in extending lifespan.

We reported a nano-Ag/liquid crystalline elastomer(LCE) nanocomposite by incorporating silver nanoparticles into a monodomain polysiloxane-based LCE matrix via a novel experimental protocol. The photo-thermo-mechanical actuation of the LCE matrix was realized via the surface plasmon resonance of silver nanoparticles while converting light into heat. The photoresponsive properties of nano-Ag/LCE nanocomposites were investigated with varying illumination intensities and silver nanoparticle doping concentrations(0.04% to 0.2%, mass fraction). The nano-Ag/LCE nanocomposites show sensitive deformation under irradiation due to their excellent photo-thermal conversion efficiency, and this photostimulated muscle-like actuation is fully reversible via the on-off behavior of light. Incorporating silver nanoparticles into the LCE matrix also improves the mechanical properties and enhances the load-actuation capability of the material.

High-fat diet (HFD) and sucrose intake can lead to hyperlipidemia, hypercholesterolemia, and nonalcoholic fatty liver disease (NAFLD) as well as disturbed gastrointestinal microbiota and dysfunctional intestinal barrier. In the present study, we showed that Ganoderma lucidum polysaccharide and chitosan (PC) significantly mitigated the hyperlipidemia in HFD-fed hamsters via lowering the contents of serum total triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and aspartate aminotransferase (AST). Furthermore, PC changed the composition of gastrointestinal microbiota and elevated the relative abundances of beneficial bacteria, such as Prevotella, Oscillibacter, and SCFA-producers. Interestingly, we also found that the abundances of Prevotella, Alloprevotella, Bifidobacterium, and Alistipes were negatively associated with serum lipid profiles. Collectively, the above-mentioned findings indicated that PC could improve lipid metabolic disorders, at least in part, by modulating gastrointestinal microbiota, suggesting that PC could be used as a potential lipid-lowering ingredient in functional foods. PRACTICAL APPLICATIONS: PC could ameliorate lipid metabolism disorder, at least in part, by regulating specific gut microbiota, suggesting its potential as a novel lipid-lowering ingredient in functional foods. We believed that our findings could be of interest to the readers because they help others further understand the gut microbiota alterations that occurred after PC supplementation in the context of metabolic syndrome (MetS).

In this paper we investigate staggered discontinuous Galerkin method for the Helmholtz equation with large wave number on general quadrilateral and polygonal meshes. The method is highly flexible by allowing rough grids such as the trapezoidal grids and highly distorted grids, and at the same time, is numerical flux free. Furthermore, it allows hanging nodes, which can be simply treated as additional vertices. By exploiting a modified duality argument, the stability and convergence can be proved under the condition that $\kappa h$ is sufficiently small, where $\kappa$ is the wave number and $h$ is the mesh size. Error estimates for both the scalar and vector variables in $L^2$ norm are established. Several numerical experiments are tested to verify our theoretical results and to present the capability of our method for capturing singular solutions., Comment: 17 pages, 27 figures

Additional file 3: Table S1. Amino acid sequences of 121 OBPs of Ceracris nigricornis and other insect species used to construct phylogenetic tree. Table S2. Amino acid sequences of 87 CSPs of Ceracris nigricornis and other insect species used to construct phylogenetic tree. Table S3. Amino acid sequences of 293 ORs of Ceracris nigricornis and other insect species used to construct phylogenetic tree. Table S4. Amino acid sequences of 115 IRs of Ceracris nigricornis and other insect species used to construct phylogenetic tree. Table S5. Amino acid sequences of 24 SNMPs of Ceracris nigricornis and other insect species used to construct phylogenetic tree.

Additional file 1: Table S1. The summary of the Illumina sequencing data. Note: FA: female antennae; MA: male antennae; FH: female head (antennae were cut off); MH: male head (antennae were cut off); FL: female leg; ML: male leg; FW: female wing; MW: male wing; FT: female abdomen-thorax; MT: male abdomen-thorax. Representation of three biological repeats with Arabic numbers 1, 2 and 3. Table S2. Length distribution and quality metrics of C. nigricornis transcripts and unigenes. Table S3. BUSCO analyzed the assembly completeness of C. nigricornis. Table S4. The summary of functional annotation of C. nigricornis transcriptomes. Table S5. Conserved domains of odorant binding proteins (OBPs) in C. nigricornis. Table S6. Conserved domains of chemosensory proteins (CSPs) in C. nigricornis. Table S7. Primers used for qRT-PCR.