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Additional file 1: Fig. S1. Regional distribution, density distribution and principal component analysis of gene expression. Fig. S2. Hierarchical clustering DEGs in CS (LTvsNT). Each column represents a comparison group, and each row represents a gene. Fig. S3. qRT-PCR validation of expression profiles obtained by RNA-Seq in CT and CS under cold stress. Table S1. Summary of sequence assembly after illumine sequencing. Table S2. Number of reads sequenced and mapped to the Brassica rapa genome. Table S3. List of the core genes set involved in CS response to cold stress. Table S4. Top30 up GO enrichment analysis in CT(LTvsNT). Table S5. Top30 up GO enrichment analysis in CS(LTvsNT). Table S6. Primer pairs used to detect the expression of selected genes.

To improve the industrial application of wheat bran insoluble dietary fiber (W-IDF), three modification methods (carboxymethylation, complex enzymatic hydrolysis, and ultrafine comminution) were compared on the basis of structural, physicochemical, functional, and antioxidant properties of W-IDF. FT-IR, DSC and SEM analysis showed that modifications contributed to alteration in morphology and arrangement of chemical bonds in W-IDF. Carboxymethylation effectively improved the water retention (WRC), water swelling (WSC), and glucose adsorption capacities (GAC); complex enzymatic hydrolysis greatly improved the oil retention (ORC), GAC, and nitrite ion adsorption capacities (NIAC). Although ultrafine comminution reduced the WRC and ORC, while positively influenced the GAC and NIAC. Moreover, total phenol content, total antioxidant capacity, DPPH radical scavenging capacity, Fe2+ chelating capacity and total reducing power were improved in modified W-IDF. Our results confirmed that carboxymethylation can improve the nutritive quality and sensory properties of W-IDF (nutritive ingredient) in food products.

A reaction-diffusion-advection model is proposed and investigated to understand the invasive dynamics of Aedes aegypti mosquitoes. The free boundary is introduced to model the expanding front of the invasive mosquitoes in a heterogenous environment. The threshold $R^D_0$ for the model with Dirichlet boundary condition is defined and the threshold $R^F_0(t)$ for the free boundary problem is introduced, and the long-time behavior of positive solutions to the reaction-diffusion-advection system is discussed. Sufficient conditions for the mosquitoes to be eradicated or to spread are given. We show that, if $R^F_0(\infty)\leq 1$, the mosquitoes always vanish, and if $R^F_0(t_0)\geq 1$ for some $t_0\geq 0$, the mosquitoes must spread, while if $R^F_0(0), Comment: 25 pages