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IntroductionThe aim of the study was to explore the clinical efficacy and safety of intravenous thrombolysis and bridging artery thrombectomy for hyperacute ischemic stroke with unknown onset time.Material and methodsOne hundred and twenty-eight patients with hyperacute cerebral infarction and without a clear time of onset were randomly divided into intravenous thrombolysis (n = 66) and bridging artery thrombectomy groups (n = 62).ResultsIn the intravenous thrombolysis group, 37 patients’ vessels had recanalization, 32 patients’ 24-hour National Institute of Health Stroke Scale (NIHSS) score improved, and 42 patients’ 90-day modified Rankin Scale (mRS) score was good. In the bridging artery thrombectomy group, 62 patients’ vessels had recanalization, 28 patients’ 24-hour NIHSS score improved, and 38 patients’ 90-day mRS score was good.ConclusionsThe benefits and adverse events between intravenous thrombolysis and bridging artery thrombectomy for ischemic stroke with unknown time of onset are similar.

The research on the temperature field of the transformer winding under dynamic variable load is of great significance for ensuring the safe operation of power systems. This paper takes an SSP-360000/500 transformer as the research object, establishes a 2-D simulation model, and uses the finite volume method to analyze the high voltage winding and low voltage winding temperature distribution of the transformer under dynamic variable load. The simulation calculation results have been fully verified by the experimental data to make the successful prediction of the overall temperature and hot spot temperature position of forced oil circulation transformers with a guided structure. The results show that the most significant temperature raise occurs at the secondary end of the winding. In the case of dynamic variable load. The temperature raise of the winding becomes larger as the load increases, but before the rated load is in the stable temperature rise range, it can run safely for a long time. However, during overload operation, the average temperature raise of the high voltage winding may exceed its limit, the insulation material is damaged.

Additional file 2. Summary of patients’ demographic data.

Additional file 1. CBCT measurement of root length, apical foramen size and root thickness preoperatively and at the one year follow-up.

Additional file 3. Preoperative measurement of root morphology by CBCT.

Additional file 4. Postoperative measurement of root morphology by CBCT at one year follow-up.

Additional file 4. Postoperative measurement of root morphology by CBCT at one year follow-up.

Additional file 1: Table S1. The clinicopathologic characteristics in 108 ESCC patients.

Additional file 3. Preoperative measurement of root morphology by CBCT.

Additional file 2. Summary of patients’ demographic data.

Additional file 3. The uncropped images of western blotting strips of 20 sera in ELISA and a positive control.

Background The genetic variation of gastric cancer has not been fully identified. We aimed to screen and identify common variant single nucleotide polymorphisms (SNPs) and long noncoding RNA (lncRNA) related SNPs associated with the risk of gastric cancer, and construct and evaluate prediction models based on polygenic risk score (PRS). Methods Non-genetic factors such as H.pylori infection, environment, and genetic factors associated with gastric cancer were screened following meta-analysis and bioinformatics,verified by frequency matched case-control study. PRS and weighted genetic risk scores (wGRS) were derived from estimation of effect size. Net reclassification improvement (NRI), integrated discrimination improvement (IDI), akaike information criterion (AIC) and bayesian information criterion (BIC) were used to evaluate model. Results A risk gradient was observed across quantile of the PRS, the results showed that the risk of gastric cancer in the highest 10 quantile of PRS was 3.24 folds higher than that of the general population (OR=3.24,95%CI: 2.07, 5.06). The PRS with one or more risk factors (smoking, drinking and H. pylori infection) was superior to the single genetic risk model. For NRI and IDI, the PRS combinations were significantly improved compared to wGRS model combinations (PH. pylori infection was the best fitting model (AIC=117.23, BIC=122.31). Conclusion Our findings indicated that the model based on PRS combined with lncRNA SNPs, smoking, drinking, and H. pylori infection had the optimal predictive ability on the risk of gastric cancer, contributing to distinguish high-risk groups from population.

Additional file 1: Table S1. The list of genes in DNA-based NGS panel.

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生态旅游服务价值作为生态系统生产总值（Gross Ecosystem Product，GEP）的重要组成部分，是生态产品价值实现的重要途径。以衢州市柯城区'一村万树'实施区为研究区域，采用国际上普遍认可的旅行费用区间法（Travel Cost Interval Analysis，TCIA）进行了问卷调查，开展了生态旅游服务的价值核算；同时基于计量经济学原理构建了游客随机效用模型（Random Utility Model，RUM），探究了不同自然地理属性对游客生态旅游选址决策的影响。结果表明：（1）2018年'一村万树'实施区的生态旅游服务总价值约19.29亿元，单位面积生态旅游价值量为8.37万元/hm2；（2）2018年'一村万树'实施区的游客人均游憩价值为713.89元，只占整个柯城区人均游憩价值（1204.58元）的59.3%，说明其还有较大的生态产品价值实现提升空间；（3）柯城区游客人均游憩价值远高于当地文化旅游局提供的621.80元的旅游人均消费支出，说明游客时间成本和消费者剩余蕴含了丰富的潜在价值，需要在评估时予以考虑；（4）随机效用模型结果显示，目的地旅行费用与游客访问呈负相关，森林和湿地面积均与游客访问呈正相关，且湿地的相对贡献远高于森林。研究结果对'一村万树'实施区有针对性地进行生态保护、管理和开发具有指导意义，对提高GEP核算的准确性和探索生态产品价值实现的机制具有借鉴意义。

More than 60% of oil-immersed current transformers are the vertical type in the Inner Mongolia Power Grid, which affects the operation stability of power system directly. This paper presents a diagnosing case of partial discharge defects of a 110kV vertical oil-immersed current transformer (VOCT). It is found that the Dissolved Gas Analysis (DGA) and dielectric loss of the VOCT exceeded warming thresholds. To explain the abnormal experimental results, byproducts in oil were examined and dielectric loss were re-measured under different voltage levels. In addition, the results of dielectric loss and grounding capacitance measurements indicated that the insulation of cable-paper tapes was good and the discharge was not attributed to moisture. Scanning Electron Microscope (SEM) and Energy Dispersive Spectrometer (EDS) showed that the copper belts and cable tapes are neither oxidized nor aged. Based on the diagnosis and analysis, the causes of defects were cleared, and technical suggestions for instrument transformer were proposed.

Canine parvovirus (CPV) has been used in cancer control as a drug delivery vehicle or anti-tumor reagent due to its multiple natural advantages. However, potential host cell cycle arrest induced by virus infection may impose a big challenge to CPV associated cancer control as it could prevent host cancer cells from undergoing cell lysis and foster them regain viability once the virotherapy was ceased. To explore CPV-induced cell cycle arrest and the underlying mechanism toward improved virotherapeutic design, we focus on epidermal growth factor receptor (EGFR), a cellular receptor interacting with TfR that mediates CPV-host interactions, and alterations on its tyrosine phosphorylation sites in response to CPV infection. We found that CPV could trigger host G1/S cell cycle arrest via the EGFR (Y1086)/p27 and EGFR (Y1068)/STAT3/cyclin D1 axes, and EGFR inhibitor could not reverse this process. Our results contribute to our understandings on the mechanism of CPV-induced host cellular response and can be used in the onco-therapeutic design utilizing CPV by preventing host cancer cells from entering cell cycle arrest.

5-aminolevulinic acid (5-ALA)-based photodynamic therapy (PDT) has been successfully used in the treatment of cancers. However, the mechanism of 5-ALA transportation into cancer cells is still not fully elucidated. Previous studies have confirmed that the efficiency of 5-ALA-PDT could be affected by the membrane skeleton protein 4.1R. In this study, we investigated the role of 4.1R in the transport of 5-ALA into cells. Wild-type (4.1R+/+ ) and 4.1R gene knockout (4.1R-/- ) mouse embryonic fibroblast (MEF) cells were incubated with 1 mm 5-ALA and different concentrations of specific inhibitors of GABA transporters GAT (1-3). Our results showed that the inhibition of GAT1 and GAT2 in particular markedly attenuated the intracellular PpIX production, reactive oxygen species (ROS) level and 5-ALA-induced photodamage. However, the inhibition of GAT3 did not show such effects. Further research showed that 4.1R-/- MEF cells had a lower expression of GAT1 and GAT2 than 4.1R+/+ MEF cells. Additionally, 4.1R directly bound to GAT1 and GAT2. Taken together, GAT1 and GAT2 transporters are involved in the uptake of 5-ALA in MEF cells. 4.1R plays an important role in transporting 5-ALA into cells via at least partly interaction with GAT1 and GAT2 transporters in 5-ALA-PDT.

A growing body of evidence suggests that long non-coding RNA (lncRNA) is aberrantly expressed in human cancer and linked to cancer initiation and development. We previously identified Homo sapiens PGM5 antisense RNA 1 (PGM5-AS1) as a novel esophageal squamous cell carcinoma (ESCC)-related lncRNA by performing high-throughput RNA sequencing. However, its clinical implication and biological function in ESCC are still uncharacterized. In the present study, we found that PGM5-AS1 was frequently downregulated in ESCC tissues, plasma, and cell lines, and low PGM5-AS1 expression was positively correlated with poor differentiation, advanced tumor node metastasis (TNM) stage, and lymph node metastasis. Importantly, PGM5-AS1 was identified to be an effective diagnostic and prognostic biomarker for ESCC patients. Functional experiments revealed that exogenous expression of PGM5-AS1 significantly suppressed the proliferation, migration, and invasion of ESCC cells in vitro as well as tumor growth in vivo. Mechanistically, PGM5-AS1 was transcriptionally activated by p53 and it could directly interact with and sequester miR-466 to elevate PTEN expression, thereby inhibiting ESCC progression. Overall, our data indicate that PGM5-AS1 is a novel tumor suppressor in ESCC and restoration of PGM5-AS1 may be a promising avenue for treatment of ESCC patient.

Main text The CCQM-K152 key comparison in parallel with CCQM-P192 pilot comparison were organised jointly by the Inorganic Analysis (IAWG) and Electrochemical Analysis and Classical Chemical methods (EAWG) working groups of CCQM to test the abilities of the metrology institutes to measure the amount content of the non-metallic elements in high purity salts as well as assaying high purity salts to be used as primary standards. Eight NMIs participated in the key comparison CCQM-K152. Ural Research Institute for Metrology (UNIIM) (since 2020 it has been called Affiliated Branch of D.I.Mendeleev Institute for Metrology (VNIIM-UNIIM)), Russian Federation, acted as the coordinating laboratory of the comparison. There were two measurands: the amount content of oxidants expressed as potassium iodate, mol/kg, and the mass fraction of iodine, kg/kg. The measurement methods used by participants for the amount content of oxidants expressed as potassium iodate were constant current coulometry and titrimetry. For the mass fraction of iodine results were provided by 5 NMIs, 3 of them used the direct approach (recalculation from coulometric or titrimetric results) and 2 NMIs implemented the indirect approach based on impurities assessment using combination of the instrumental methods (ion chromatography, ICP OES, ICP MS). In general, good overlap of results was observed for both measurands. Direct and indirect approaches for purity determination of pure salt provide overlapping results when impurities are summarized in ionic form. The suitability of coulometry for assay of high purity materials was demonstrated again, and some possible technical problems were highlighted. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCQM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

Esophageal Cancer estimated leading deaths are more than 400,000 every year in worldwide, while 80% due to esophageal squamous cell carcinoma. In worldwide, esophageal cancer leading death ranks in sixth among cancer leading causes of death, while the 5-year survival rate of esophageal cancer is 19%. Oridonin, a tetracyclic diterpenes compound, has been indicated the anti-tumor activity for digestive system tumors. To assess the anticancer activity of oridonin on esophageal cancer, we performed cell proliferation experiment of oridonin on esophageal squamous cell carcinoma cell line TE-8 using SRB assay. We found that IC50 of oridonin on TE-8 treated for 72h is 3.0μM, which is better than positive control Gemcitabine treatment on TE-8 for 72h (5.7 μM), as well as TE-8 cells shrunk and floated with increasing concentration on oridonin. At the same time, oridonin induced a dose-dependent inhibition of cell colony formation of esophageal cancer cell line. Colony numbers of TE-8 cell line treated with 1μM and 2μM oridonin are 111 and 54, respectively, which are significantly different (p Citation Format: Xiaojun Zhang, Mengtao Xing, Yangcheng Ma, Xiao Wang, Jianying Zhang. Oridonin inhibits cell growth of esophageal carcinoma cells via induction of apoptosis [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 1854.

Women with intellectual and developmental disabilities, or IDD, face significant health, and socioeconomic disparities during pregnancy and childbirth. Pregnant women with, IDD tend to be younger and less educated than their counterparts without disabilities, and are less likely to be married.1,2 They are also more likely to use public health, insurance and to smoke during pregnancy. 1,2 Furthermore, mothers with IDD are at an, increased risk for complications during pregnancy, including pre-eclampsia, hypertension, premature labor, venous thromboembolism, and peripartum, hemorrhage. 1,2,3,4,5 To identify some of these disparities between the pregnancy, experiences of women with and without intellectual disabilities, we conducted a study, of pre-childbirth hospitalizations among women with and without IDD using the, Massachusetts Pregnancy to Early Life Longitudinal Data System (PELL).

This present study attempts to conduct a comparison of the tripartite model of L2 Motivational Self System in two different learning contexts, Chinese EFL and ESL learning settings. It was designed using a mixed methods approach, with a primary questionnaire instrument being supported by secondary interview data. The quantitative data was submitted to Amos version 18.0 to evaluate proposed models. It was found that L2 learning experience was the most important predictive dimension of intended learning effort for both samples. However, the roles of the other two components, the ideal L2 self and the ought-to L2 self, appeared to be unstable, which concludes that Dörnyei’s tripartite model obtained partial support in both EFL and ESL contexts. The analysis of qualitative data offers strong evidence for the claim that the future self-guides do not necessarily result in intended effort unless some essential conditions are met.

To explore the technique principle of PCR with confronting two-pair primers (PCR-CTPP) and improve the accuracy of SNP genotyping by taking the 1298 locus of human gene MTHFR as an example, the reliability between conventional PCR-CTPP and improved PCR-CTPP was compared using reconstructed PCR-CTPP detecting system in terms of designing appropriate primers and optimizing annealing temperature and the final concentration of primers. The improved PCR-CTPP detection system proved to be more accurate, which supported the viewpoint on the theoretical defects of conventional PCR-CTPP. The large-scale study verified the reliability of the improved method. It is expected that this improved technique would be widely used in the field of medicine and molecular biology.

Although the incidence rate of lung cancer has declined over the past few years, it remains the leading cause of cancer death worldwide. The Cancer Genome Atlas has provided invaluable resources for cancer research. The development of next generation sequencing technology and evolution of data analysis tools make it possible to interpret the underlying mechanism of oncogenesis. In current study, normalized FPKM data from 572 samples derived from patients with lung adenocarcinoma (LUAD) from TCGA was used to do weighted gene co-expression network analysis (WGCNA) to identify gene modules associated with lung adenocarcinoma. Raw counts data was used to identify differentially expressed genes in LUAD by using DESeq2 in R (version 3.4.2). WGCNA results shows that magenta (r = 0.47, p Keywords: lung adenocarcinoma; TCGA; gene expression analysis; bioinformatics; cancer biomarker Citation Format: Jianxiang Shi, Mengtao Xing, Chenglin Luo, Xiao Wang, Liping Dai, Jianying Zhang. Revisiting TCGA data identifies key genes in lung adenocarcinoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3292.

The South Lake Taihu region is increasingly threatened by water eutrophication,so it is important to understand phosphorus status in sediments and water of the riparian wetlands in this region and develop sound technologies for optimizing wetlands'ecological values.29 typical sampling sites in this region were chosen for the investigation,and the sites located at Hangzhou West Lake,Deqing Xiazhuohu Wetland,and Changxing Baoyanghe River were selected for static column simulation experiments and steady-flow flume simulation experiments.According to the investigation,total phosphorus(TP)and NaHCO_3 (pH=8.5)extractable phosphorus(Olsen-P)in sediments of the region reached 0.169-1.200g/kg and 7.08-67.08mg/kg,respecially, and the adsorption capacity(Q_(max))of sediments varied from 269.5 to 824.Smg/kg.NaOH+EDTA extractable phosphorus (NaOH+EDTA-P)constituted 52.0%±11.6% of TP,while Olsen-P was increased as organic phosphorus accumulated.TP in overlying water was 0.036-0.944mg/L,while dissolved reactive phosphorus(DRP)was relatively and 34.5% of the total sites was below detective limits.Total particle phosphorus(TPP)contributed 70.9%±13.2% of TP in water samples.There was no significant relationship between TP,DRP in overlying water and TP,NaOH+EDTA-P,Olsen-P in sediments,implying that phosphorus status in sediments was not the only factor restricting phosphorus in overlying water.In the static column simulation experiments,DRP in overlying water decreased slowly at the beginning,then decreased fastly later on.A quasi U-pattern curve was found in the steady-flow flume simulation experiments,i.e.DRP in the overlying water decreased at first and then turned to be increasing.It indicated that the role of the sediments might be changed from phosphorus"sink"to"source"when high phosphorus water flow over a wetland in rain season.It was also proved in the simulation experiments that organic matter accelerated the adsorption rates of phosphorus by sediments.

This paper reports two new species of Euryhaliotrema Kritsky & Boeger, 2002 collected from the gills of Lutjanus argentimaculatus (Forsskal). E. xinyingense n. sp. is similar to Euryhaliotrema atlanticum Kritsky & Boeger, 2002 in the structure of copulatory apparatus, but differs from the latter in the shape of anchors and the structure of vagina. E. hainanense n. sp. also closely resembles E. atlanticum Kritsky & Boeger, 2002, but differs from the latter in the structure of haptor and the shape of accessory piece.

Sinomazocraes changjiangensis n. g., n. sp. (Monogenea: Mazocraeidae) is described from the gills of the shortjaw tapertail anchovy Coilia brachygnathus collected from Jiayu and Huanggang Counties (Hubei Province), which are located in the middle reaches of the Yangtze River. The structure of the clamps and copulatory organ are similar to those of Heteromazocraes Mamaev, 1981. However, although Sinomazocraes also has four pairs of clamps, it is distinguished from other genera in the family by the fact that the three anterior clamps on one side of the body are significantly larger than the others. Variations in the form of the clamps in different species of the Mazocraeidae are discussed.

This paper contains a brief history of studies on monogeneans of Chinese marine fishes and a list of 337 monogenean species, together with their marine hosts and notes on the sea in which they were found. An additional 139 species have been added to the 198 species recorded in 'Monogeneans of Chinese Marine Fishes' published by Zhang, Yang and Liu in 2001.

The evaporation of a small liquid drop on a solid substrate is not only a fundamental phenomenon in our daily life, but also of particular importance in a number of applications, such as particle synthesis, DNA/RNA arrangement and disease diagnosis. This phenomenon has been extensively discussed by many researchers, however, most of these studies considered only drops that are smaller than the capillary length (e.g., 2.7 mm for water droplets), and the gravity effects were thus neglected. So we attempt to make a detailed investigation of the gravity effects on the liquid drop evaporation on a horizontal substrate using the lattice Boltzmann method. In order to account for the variation of evaporation flux along the drop interface, an evaporation scheme is introduced into the framework of the multiphase lattice Boltzmann method. Our code is validated by some simple cases, and the simulated values agree well with analytical values. Then the drop shape changing rules and inside flow patterns of drops with or without gravity during evaporation at different contact angles are studied. The results show that the gravity impact decreases as the drop size decreases. When the drop size is lower than a critical value, the gravity impact is negligible, which is in accordance with our common sense. However, these critical values for water droplets with different wetting properties are found at least 25% lower than the value given by the classical theory of capillary. Therefore, many previous studies may need to be reconsidered.Copyright © 2014 by ASME

The genera Sinineobucephalopsis and Sinogastromyzontrema are diagnosed and placed in the family Urotrematidae. Sinineobucephalopsis macrocirrus is redescribed from Glyptothorax fokiensis in Guangdong and Hunan provinces. Urotrema glyptothoraci is considered synonymous with Sinineobucephalopsis macrocirrus. The new combinations Sinineobucephalopsis postlecitha, Sinineobucephalopsis proeilecitha and Sinineobucephalopsis sinipercae are formed for species originally placed in Urotrema and Urotrematulum. Sinogastromyzontrema guangxiensis is redescribed from Sinogastromyzon wui in Guangxi Province. Urotrema parallelorchis is considered a synonym of Sinogastromyzontrema guangxiensis. A key to the species or species-complexes in the family Urotrematidae is given.

This paper reports two new species of the family Mazocraeidae from the Pearl River Estuary and the Xijiang River, China. Mazocraeoides xijiangensis n. sp. is found on the gills of Macrura reevesi (Richardson) and Heteromazocraes hexacanthus n. sp. on the gills of Coilia grayi Richardson. A new species name, Heteromazocraes lingmueni, is given to replace Paramazocraes sp. of Ling (1973).

This paper reports a new species and two new records of the Tetraonchoididae collected from fishes of the South China and East China Seas. Pseudotetraonchoides halichoeres n. sp. was obtained from the gills of Halichoeres poecilopterus collected at Dongshan (23°42' N, 117°24' E). The new species is similar to P. bleekeriae Bychowsky, Gussev & Nagibina, 1965 in the structure of the haptor, but differs in the sizes of the hamuli, the transverse bar and the supplementary plate, and also in details of the supplementary plate. Both Heteropavlovskioides synodontis Machida, 1978 from Trachinocephalus myops and Tetraonchoides japonicus Bychowsky, 1951 from Uranoscopus japonicus are recorded for the first time off China.

The rural sci-tech supermarket in Jiangsu Province is an innovative model of rural sci-tech service. With reference to the concept of modern commodity supermarket, this model integrates variety, technology and achievement information into the sci-tech supermarket platform, provides various agricultural sci-tech services, and improves farmers’ recognition and acceptance of science and technology, so as to accelerate conversion of scientific and technological achievements and demonstration and popularization of new varieties, new technologies and new models. This paper analyzes practice of Jurong economic forest and fruit industrial branch and presents recommendations for building Jiangsu rural sci-tech service supermarket.

Natural killer (NK) cells are innate lymphocytes that can rapidly eradicate tumor cells, especially those lacking MHC Class I molecules. NK cells can also rapidly eradicate herpes virus-infected cells. We designed an oncolytic herpes virus (oHSV) to selectively infect, replicate within, and lyse glioblastoma (GBM), a devastating brain tumor with a median survival of only 15 months following diagnosis. We have shown that the rapid influx of NK cells limits oHSV efficacy in GBM as they impede oHSV replication and spread [Alvarez-Breckenridge et al., Nat Med, 2012, 18(12):1827-34]. In the current study, we developed NK cell-based novel GBM therapies by decreasing the brain influx of NK cells to enhance the efficacy of oHSV, while arming NK cells in the brain with a chimeric antigen receptor (CAR) that targets both the wild-type EGFR and its mutant form EGFRvIII, two GBM tumor-associated antigens. We then investigated the synergistic effects between EGFR-CAR NK cells and oHSV. Transforming growth factor (TGF)-β is a potent immunosuppressive cytokine of NK cells [Yu et al, Immunity, 2006, 24(5):575-90]. We first determined if oHSV efficacy for treatment of GBM would be augmented by inhibiting anti-oHSV activity of NK cells with TGF-β pre-treatment. In vitro, NK cells pre-treated with TGF-β displayed less cytolytic capacity against oHSV-infected GBM cell lines and patient-derived GBM stem-like cells. In viral replication assays, co-culturing oHSV-infected GBM cells with NK cells pre-treated with TGF-β significantly increased virus titers. In an immunocompetent syngeneic GBM mouse model,administration of TGF-β to GBM-bearing mice prior to oHSV injection significantly inhibited intracranial infiltration and activation of NK cells (P < 0.05). In orthotopic human GBM xenograft mouse models and in syngeneic GBM mouse models, TGF-β treatment in vivo prior to oHSV therapy resulted in inhibition of NK cell infiltration, suppression of tumor growth and significantly prolonged survival of GBM-bearing mice (P < 0.05). Furthermore, depletion of NK cells incompletely blocked the positive effects of in vivo treatment of GBM with TGF-β on survival, suggesting that TGF-β may also directly act on other innate immune cells such as macrophages/microglia. These data demonstrate a single dose of TGF-β prior to oHSV administration enhances anti-tumor efficacy for GBM at least in part through the transient inhibition of the innate immune responses to oHSV infection. We next investigated whether NK cell activity could be enhanced to more directly target brain tumors while sparing eradication of oHSV. We therefore infected both human NK-92 cells and primary human NK cells to express the second generation CAR targeting both EGFR and EGFRvIII that we designed. Further, we asked if the treatment with EGFR-CAR NK cells plus oHSV could create a therapeutic synergy for the treatment to brain tumors. In vitro, compared with mock-transduced CAR-NK-cells, EGFR-CAR NK cells exhibited significantly higher cytotoxicity and IFN-γ production when co-cultured with tumor cells, for both NK-92 and primary NK cells (P < 0.01). Further, significantly higher cytolytic activity against tumor cells was obtained when CAR NK cells were combined with oHSV-1 infection of tumor cells, compared to either of the monotherapies alone (P < 0.05). In mice, to avoid oHSV clearance by the EGFR-CAR NK cells following the inoculation of the mouse with tumor cells, we administered these two agents sequentially; administering EGFR-CAR NK cells directly into the tumor first as a single injection of 2 × 106 cells, followed by intracranial infection with 2 × 105 plaque-forming units oHSV five days later, presumably after EGFR-CAR NK survival has diminished. Compared to vehicle controls, intracranial administration of either EGFR-CAR NK cells or oHSV blunted tumor growth. However, the combination of EGFR-CAR NK cells followed by oHSV infection resulted in significantly more efficient killing of tumor cells (P < 0.05) and significantly longer survival for tumor-bearing mice when compared to either monotherapy alone. Collectively, our studies demonstrate that in animal tumor models, we can combine novel NK cell and oHSV therapies to significantly improve survival. Disclosures No relevant conflicts of interest to declare.

Liquid chromatography-atmospheric pressure chemical-ionization mass spectrometry (LC-APCI-MS) was used for the analysis of N-acetylamino acids that could not be determined with an amino acid analyzer. LC-APCI-MS could directly detect the protonated molecular ions of various synthetic N-acetylamino acids, distinguishing N-acetylserine from O-acetylserine and N(alpha)-acetyllysine from N(epsilon)- acetyllysine. Furthermore, N-acetylasparagine, N-acetylaspartic acid, N-acetylglutamine and N-acetylglutamic acid were identified in normal human urine. The assay data for N-acetylaspartic acid agree with a previous report using gas chromatography-mass spectrometry. These results demonstrate the usefulness of the apparatus described above for the analysis of N-acetylamino acids in biological samples.

The vibration of flexible systems can be restrained by using of the component synthesis active vibration suppression method (CSVS). While the number of flexible vibrations increases, the number of the components increases, rapidly. In this paper on the basis of CSVC method, a zero-placement technique is applied for construction of component forces to suppress multi-harmonics, simultaneously. The nature of zero-placement method is to put enough number of zeros to cancel system poles at necessary points. The designed synthetical force has equal time intervals between each component. Using this method the number of components increase with increasing of number suppressed harmonics, linearly. Simulation verified the availability and superiority of zero placement method.

The structural vibration of flexible systems can be reduced using the component synthesis vibration suppression method (CSVS), but the vibration caused by exterior disturb can not be reduced. This paper studies the problem of concurrent design of the CSVS method and feedback control based on proportion and velocity feedback controller for optimizing system performances. For a flexible single beam model, the numerical simulation indicates that settle time was reduced obviously against the sequence design method, without increase of system overshoot. These results validate that the concurrent design method can be used to achieve optimal performance.

As the foundation of motion control, driving dynamics equations with velocity and geometric constrains are given firstly. A method to increase tractive ability and the concept of the corresponding lunar rover chassis are proposed. The simulation results are given to express the effectiveness of increasing tractive ability. A control method was suggested which combined drive and guidance system to form a closed control system and an observer will work out the skid values and wheel-terrain contact parameters by using the measured data of passed route of lunar rover's mass center. This method ensures more accurate and safely movement and increase driving control quality.

For long range targeting applications with significant ballistic coasting after burnout, determination of the zero effort miss must account for the different gravitational accelerations experienced by each vehicle. A new expression of relative motion is derived using the Newton's binomial theorem and modern control theory, then the final time at miss is calculated by Newton iteration, and a new predicted method of zero effort miss is obtained. In order to validate the fidelity of proposed zero effort miss, several methods of determination of the zero effort miss are compared with proposed method by simulation. Simulation results show that zero effort miss formulation better than other methods and has obvious advantages of the application.

Four species of the Monogenoidea, Laticola lingaoensis n. sp., L. latesi (Tripathi, 1957) n. comb. [previously Pseudorhabdosynochus latesi (Tripathi, 1957) Kritsky & Beverley-Burton, 1986], L. paralatesi (Nagibina, 1976) n. comb. [previously Diplectanum paralatesi Nagibina, 1976] and Diplectanum penangi Liang & Leong, 1991, are reported from the gills of Lates calcarifer (Centropomidae) from the South China Sea (new geographical records for L. latesi and D. penangi). Collections from off Bathurst Island, Northern Territory, Australia, represent a new geographic record for L. paralatesi; Chilka Lake, Orissa, India, is established as the type-locality for L. latesi. Laticola n. g. (Diplectanidae) is proposed for species with a spoon-shaped copulatory organ with two to four concentric incomplete ridges in the base. Laticola lingaoensis, the type-species of Laticola, is described, and L. latesi and L. paralatesi are redescribed based on specimens from the South China Sea. Pseudorhabdosynochus monosquamodiscusi Balasuriya & Leong, 1995 and Pseudorhabdosynochus yangjiangenesis Wu & Li, 2005 are considered junior subjective synonyms of L. latesi and L. paralatesi, respectively.

An experimental animal model with cystathioninuria was obtained by the injection of D,L-propargylglycine into rats. The concentrations of D,L-propargylglycine in urine, several tissues and serum at different times after the injection were measured by liquid chromatography-mass spectrometry. The propargylglycine accumulated rapidly in several tissues and serum of the rats, and reached its maximum level at about 2 h after the injection. Approximately 21.2% of the administered propargylglycine was excreted in urine. N-Acetylpropargylglycine was identified as a new metabolite of propargylglycine in urine. The concentration of propargylglycine was 100 times that of N-acetylpropargylglycine in urine.

Iminodipeptides containing C-terminal proline or hydroxyproline were determined in sera from patients with prolidase deficiency, in their mother's serum, and in the sera of unrelated controls, using liquid chromatography-mass spectrometry with an atmospheric pressure ionization interface system. The separation was carried out on a reversed phase column using 1 g/l aqueous trifluoroacetic acid-methanol (75 + 25, by vol.). The quasi-molecular ions ([M + H])+ of various iminodipeptides containing C-terminal proline and hydroxyproline were observed in the sera of patients with prolidase deficiency, using selected ion monitoring. The quasi-molecular ions ([M + H])+ of iminodipeptides containing C-terminal proline were not observed in the sera of normal subjects or the patients' mother, but the latter did contain various iminodipeptides with C-terminal hydroxyproline. This method proved useful for the determination of iminodipeptides in the sera of patients with prolidase deficiency.